PerkinElmer LAMBDA 265 User manual

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PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
Lambda 265 Peltier Controlled Single Cell,
With Peltier Controller Installation Instructions
This instruction sheet describes the installation of this accessory which is used with the Lambda 265
Spectrophotometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
•Easy to install
•Full software control
•Liquid cooling system
•N2 gas available
Figure 1. Lambda 265 Peltier control Single Cell [P/N: N4103005] and peltier
temperature controller [P/N: 4100040]

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gs
Dimensions and Specifications
Dimensions of Peltier Controller
Physical Characteristic Specification
Power
100-240 VAC, 50/60 Hz, 300W, Free Voltage
Temperature Range
-5 to 100°C (Maximum Internal Temperature)
Maximum Ambient Operating Temperature 40°C
Dimensions
310(W) x 410(D) x 275(H) mm
Weight
20.0 Kg
Coolant Volume
2 L
N
2Gas Pressure Limit
100 psi (6
.9 bar)
Liquid Cooling System
Magnetic Stirrer Control Available
Emer
gency Power Off Switch Available
Specifications of Peltier Controlled Single Cell
Physical Characteristic Specification
Dimensions 65(W)x240(D)x114(H)mm
Weight 1.4 Kg
Temperature Accuracy ±0.15°C
Temperature Precision ±0.1°C
Temperature Stability ±0.1°C
Ramping Time (from 25 to 100°C)4 minutes

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Safety Warnings
WARNING
When this label is attached to an instrument it means refer to the
manual.
Lorsque cetteétiquette est attachéeà un instrument, il est nécessaire de
voir le manuel.
See the following figure for the location of warning labels on the back of the instrument:
Warning
To prevent electrical shock,
do not open cover.
Refer all servicing to qualified
personnel.
Avertissement
Afin d’éviter tout risque
d’électrocution ne pas enlever
ce couvercle.
Faire appel au service Après-
Vente.
Warning
For continued protection against electrical
shock, replace with same type and ratings of
fuse
Avertissement
Pour assurer la protection contre le risque
de choc électrique, remplacez le fusible
seulement avec le même type et la même
valeur nominale.
Warning
FUSE
Avertissement
FUSE

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Description
Peltier Controlled Single Cell
Figure 1 Lambda 265 Peltier Controlled Single Cell
a. Interface connector between Peltier Controlled Single Cell and Peltier Controller
b. Coolant Inlet/Outlet Quick Coupler
c. N2Gas fitting
d. Interface slot for Temperature Probe
e. Cell Lifting knob
f. Temperature Probe
Figure 2 Temperature probe Accessory

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Front View of Peltier Controller
Figure 3 Front view of Peltier Controller
a. Manual touch pad with Display:Temperature Control Unit (Heating/Cooling Block)
b. LED Indicators
Display the status of the operation of Coolant Circulation, Fan, Pump, etc. (If there is any
problem in the components, Red LED will flash with a beep alarm.).
c. Manual ON/OFF button
As the Manual ON/OFF button is ON, the temperature can be controlled manually by a)
Manual touch pad with Display.
NOTE:
Manual operation is used only for the manufacturing process so it should be turned off during the
measurement.
d. N2gas ON/OFF button:It is used for using N2gas to remove the frost from the heating
and cooling.
e. Stirrer ON/OFF button: It is used for controlling of the stirring.
f. Stirrer Speed Controlling Knob: Control the stirring speed (rpm).
g. Emergency switch: Stop all operations in the emergent situation.
NOTE:
During the operation, Emergency switch should be turned off.
h. Coolant Inlet

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Rear View of Peltier Controller
Figure 4 Rear view of the Peltier Controller
a. RS-232 port
b. AC POWER: Main Power ON/OFF
c. Fuse: AC socket + Fuse Holder
d. Interface: Interface cable for connecting with Peltier controlled Single Cell
e. Air Vent Manual button: Used for removing the air in the coolant hose.
f. Buzzer: It makes an alarm sound. If there is any problem before the measurement, the
buzzer beeps 6 times at every 10 sec. If the malfunction occurs during the operation, the
buzzer beeps twice at every 10 sec.
g. Quick Coupler of Coolant lnlet/Outlet
h. Quick Coupler of Coolant Drain
i. N2Gas Inlet/Outlet ports
NOTE:
N2gas is not always required [Tube for N2 gas is not supplied with the accessory].
j. Flow Gauge: Indicator of the flow rate of N2gas.
Configuration of the equipment
Peltier controlled Single Cell
Peltier Controller
Power Cord, 3ea
Interface Cable (RS-232 and USB to RS-232), 1each
Coolant, 2ea
Coolant Hose, 1ea
Magnetic Stirrer, 1ea

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Spare Fuse (AC 250V T5AL), 2ea
Temperature Probe, 1ea
Waste Basket for Coolant, 1ea
Waste Hose for Coolant, 1ea
Installation
1. Place the Lambda 265, Peltier controller and Peltier controlled single cell in a location that is
compatible with the required environmental conditions for the operation.
2. Open the lid of the coolant inlet on top of the Peltier controller.
Figure 5 Lid of coolant inlet
3. Fill up the liquid coolant using the funnel.
CAUTION
ATTENTION
The liquid coolant must be used as refrigerant.
Do not use any water (tab
water, DI, etc).
In case that the water is used as refrigerant, Peltier
Controller’s components might be corroded and its performance may be
deteriorated.
Use any kind of liquid coolant in normal grade as is available (e.g. liquid coolant
including Distilled Water 70 wt%, Propylene Glycol 27 wt%, Additives 3 wt%).
Le liquide de refroidissement doit être utilisé comme réfrigérant.
N'utilisez
jamais d'eau (eau du robinet, eau déminéralisée, etc.)
L'eau risque en
effet de corroder les composants du contrôleur Peltier et de réduire les
performances de ce dernier.
Utilisez un liquide de refroidissement du commerce de qualité normale (par
exemple, un liquide de refroidissement à base d'eau distillée à 70 % M/V, de
propylène glycol à 27 % M/V et d'additifs à 3 % M/V).
CAUTION
ATTENTION
Make sure the instrument is turned off while installing this accessory.
Assurez-vous que l’instrument est é
teint lors de l’installation de cet
accessoire.

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NOTE: Keep the amount of liquid coolant to the level where the indicator is located between the L (low) and
H (high) mark in the scale on the left side of the Peltier Controller. When the coolant is running short for
the operation, LED of low water level is changed to red with an alarm sounding.
4. Disassemble the existing cell holder.
Figure 6 Removing the existing cell holder
5. Install the Peltier controlled single cell.
Figure 7 Installing the Peltier Controlled Single Cell

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6. Connect the accessory interface cable of the Peltier controller to the interface connector of the
Peltier controlled single cell.
Figure 8 Connecting the accessory interface cable of the Peltier Controller to the
interface connector
7. Connect the coolant inlet/outlet and N2gas tube between the Peltier controller and the Peltier
controlled single cell.
Note:
N2gas is not always required [Tube for N2 gas is not supplied].
Figure 9 Connecting the coolant inlet/outlet and N2 gas tube
8. Connect the power cord and communication cable to the ports located at the rear of the Lambda
265.

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9. Connect the temperature probe to the interface slot of the Peltier controlled single cell.
Figure 10 Connecting the temperature Probe to the interface slot of the Peltier
Controlled Single Cell
10. Connect the Peltier controller with the PC via the RS-232 or USB to RS-232 cable. See
Setting the
USB to RS-232 Port
on page 15 for port settings when you use the USB to RS-232.
Figure 11 Connecting Peltier controller with the PC
11. Connect the power cord to the Peltier Controller.
Figure 12 Connecting the power cord to the Peltier controller

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12. Turn on the power of the Lambda 265 and the Peltier Controller.
Figure 13 Back of the instrument connections
CAUTION
ATTENTION
The Air vent manual button should be off before turning on the main power of the
Peltier Controller and also during the operation since it could make a trouble for the
communication between Lambda 265 and Peltier Controller.
Le bouton d'actionnement manuel de la ventilation doit être désactivé avant que le
contrôleur Peltier ne soit mis sous tension et pendant le fonctionnement de ce
dernier afin d'éviter qu'une erreur de communication entre le Lambda 265 et le
contrôleur Peltier ne se produise.
13. Check that the Power LED is on as a blue light.
Figure 14 Power LED is on
Peltier controller auto tuning setup
Peltier controller can be compatible with various peltier cell holders (Single or Multi) so whenever you
exchange the existing peltier cell holder to the other one, you should proceed “AUTO TUNING” set up to
minimize the temperature fluctuation at the target temperature

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1. Push the Manual button on the control panel of the peltier controller then button light is on.
2. Set any SP temperature between 30~37°C using the button.
3. Press and hold the SET/ENT button until shown “G.AT”.
4. Push the SET/ENT button again.

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5. Push the button (OFFON).
6. Press the SET/ENT button, MAN LED will blink.
7. MAN LED will be off after few minutes.
8. Push Manual Button (Stop) and then light is off.

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CAUTION
ATTENTION
If you hear the alarm sound during the operation, push the emergency switch to
stop all functions immediately and check the status of Peltier Controller carefully.
Then, turn the switch to the right again when everything is solved properly and
the functions will be on again.
Si l'alarme se déclenche en cours de fonctionnement, appuyez sur l'interrupteur
d'urgence pour arrêter immédiatement toutes les fonctions et contrôlez
soigneusement l'état du contrôleur Peltier. Ensuite, tournez à nouveau
l'interrupteur vers la droite une fois la situation rétablie afin de restaurer les
fonctions.
Figure 15 Emergency switch
14. Turn off the power after the experiment.
How to Drain the Coolantfrom the Peltier Controller
a. Push the Air Vent Manual button to be switched on the rear panel of Peltier Controller before
operation.
b. Connect the wastehose to the Coolant Drain port on the rear panel and prepare a waste bucket.
c. Turn on the power of Peltier Controller, and then coolant will be drained automatically.
d. Once draining is complete, turn off the power of Peltier Controller.

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Setting USB to RS-232 Port
Use the following procedure to change the port setting when communication is not connected by using
USB to RS-232 convert.
1. Select My Computer -> Properties.
2. Select Device Manager.

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3. Select Ports (COM & LPT) to expand the listing. These are the devices currently connected to
the COM ports. USB Serial Port (COMx) is visible when the driver installation is completed
successfully.
4. Double click on USB Serial Port (COMx) of the Ports (COM & LPT) section.

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5. Select the Port Settings tab and click on the Advanced… button.
6. Change the parameter values as shown below.

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7. Select OK after checking the changed parameter values.
8. If the Peltier controller fails to communicate with the PC, change the COM Port Number in the
following steps.
9. Open Advanced Setting for COMx window following steps 1 to 6 in this procedure.
10. Select the COM Port number list to expand it and change the COM port number another one
which is not in use from COM 1 to COM 10.

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11. Make sure that the changed COM Port Number is applied, and select OK.
12. After the port setting is changed, restart the computer.
Measurement
This accessory can be operated in the Temperature Based Kinetics in the Kinetics mode and Thermal
Denaturation in the Bio Analysis mode.
Temperature Based Kinetics
1. Launch the UV Lab software and then, select Temperature Based Kinetics in the Experiment
Type.

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2. The following dialog box will be displayed.
3. Setup test parameters as follows;
a. Edit Heating (Cooling) Rate (°C/min): Set the heating (cooling) temperature rate in each
temperature range. Sample is measured whenever it reaches the set temperature by the Rate
(°C/min), and the sample is also measured at the Start Temperature and the End Temperature.
Click on Insert and Delete to edit the temperature range and the rate.
- Start Temperature: Enter the start temperature for the measurement.
- End Temperature: Enter the end temperature for the measurement.
- Rate (°C/min): Enter the heating (cooling) rate.
b. Holding Time: Enter the holding time before sample measurement after it reaches the each
set temperature.
c. Rate calculation Type: Select the rate calculation type. See V-3. Kinetics Mode of Lambda
265 UV Lab Software Users Guide for more information.
d. Calculation Start Temperature: Enter the start temperature for the calculation.
e. Calculation End Temperature: Enter the end temperature for the calculation.