PerkinElmer Lambda 465 User manual

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PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
Lambda 465 Fiber Optic Probe Installation Instructions
This instruction sheet describes the installation of this accessory which is used with the Lambda 465
Spectrophotometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Introduction
The probes provide a convenient way of performing absorption or transmission measurements by dipping
or inserting the probe end into the medium or environment. This accessory is especially useful for
embedding into process streams for real-time, on-line sample monitoring.
Features
•Easy to install
•Dip Probe for measuring liquid samples without using a cell
Figure 1 Lambda 465 Fiber Optic Probe [P/N: N4104012]
with Fiber Optic Probe [Part No: N4101048]

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Dimensions and Specifications
Physical Characteristic Specification
Fiber Optic Probe
A: Fiber Type Fused Silica
B: Fiber Size 600 µm
C: Connector SMA 905
D: PVC Jacket ∅0.19’’ OD
E: Path Length 10 mm
Spectral Range 200 nm ~ 1100 nm
Overall Length (OAL) 100 ~ 120 cm
Breakout Length (BOL) 18 ~ 23cm
Probe Length (PL) 15.2 cm
Fiber Coupling Mount Dimension (mm) 150(W) X 100(H) X 84(L)
Weight (Kg) 0.75
Description
Configuration of the Fiber Optic Probe
Figure 2 Lambda 465 Fiber Optic Probe include Fiber Coupling mount

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Installation
1. Disassemble the existing cell holder.
Figure 3 Location of the two knob bolts
2. Install the fiber optic probe by fastening the knob bolts.
Figure 4 Installing the fiber Optic probe
3. Connect the communication cable and the power cord, and then turn on the power of the Lambda
465.

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4. Place the probe into the liquid sample.
Figure 5 Placing the probe in the liquid
Measurement
NOTE: Start the sample measurement after warming up at least 20 minutes.
1. Launch the UV Lab software.
2. Choose the Fiber Module in the Accessory Type.
3. After dipping the probe into the blank solution, select the intensity mode, and then click Blank. By
changing the Integration no. up to 30000, adjust the D2 peak intensity not to be saturated in the
spectrum as shown in the picture below.

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4. Select the Absorbance mode and measure blank and sample.
Before measurement, make sure that bubble does not exist at the mirror side of the probe.
5. Clean the probe with D.I water after the measurement.
Troubleshooting
When the Intensity value is low
1. Make sure that bubble does not exist at the mirror side of the probe.
If bubbles existed, the intensity of the light is low due to scattering of bubbles. You should remove
the bubbles completely and perform measuring.
2. Increase the Integration Number in the UV Lab Software
You should increase the Integration Number since the intensity of the light which passes through the
fiber is somewhat weak.
3. Check the condition of the fiber optic probe
Check if the lens placed inside the fiber optic probe is contaminated.
Remove the contaminant with alcohol.

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