Phenomenex Presston 1000 AH1-7033 User manual
Expires: 15 July 2011
Mention offer code: ZEB8400
www.phenomenex.com/Presston
Positive Pressure Manifold
User Guide
Part Number: AH1-7033
2Phenomenex
l
WEB: www.phenomenex.com
Table of Contents for Presston™1000
Specifications..............................................................3
Safety Precautions ....................................................3
Overview ..................................................................4
Overview of Front ....................................................4
Overview of Back ....................................................5
Accessories ...............................................................6
Set-Up ....................................................................7
Unpacking ..........................................................7
Gas Regulator Installation...............................................7
Gas Flow Control .....................................................8
Operation .................................................................9
Getting Started.......................................................9
Maintenance ..............................................................11
Gas Purification .....................................................11
Cleaning...........................................................11
Gasket Replacement .................................................11
Manifold Shield Positioning.............................................11
Warranty Information .................................................11
Tips .....................................................................12
FAQs ....................................................................13
If you have any questions while working with your Presston 1000, please email
© 2019 Phenomenex. All rights reserved.
3
Phenomenex
l
WEB: www.phenomenex.com
Safety Precautions
Before installation and operation of the device, please read the safety precautions carefully. Presston 1000 is
designed for laboratory use only and for trained personnel.
• IMPORTANT - do not insert hand between the manifold shield and the
manifold spacers or 96-well plate product.
• Always handle corrosives in a well-ventilated environment.
• Always wear personal protective equipment.
96-Well Plate Positive Pressure Manifold
Part No.: AH1-7033
Weight: 15 kg (33.1 lbs)
Number of Samples Processed: 96 Samples
Dimensions: 301 x 382 x 377 mm
11.8 x 15 x 14.8 in.
Flow Control: Adjustable flow control (total flow and
individual flow)
Gas: Nitrogen or compressed air (43-145 psi)
Gas Supply Pressure: Dependent on gas system
Gas Regulator Pressure: 0.3-1 MegaPascal (MPa)
3-10 Bar
43 -145 psi
Low Pressure Regulator: 0-0.1 MPa
0-1 Bar
0-14.5 psi
High Pressure Regulator: 0-0.4 MPa
0-4 Bar
0-60 psi
Gas Flow Meter: 3-12 L/min
Operating Temperature: Room Temperature
Specifications of Presston 1000
4
Instructions
Phenomenex
l
WEB: www.phenomenex.com
Overview
Easily streamline your 96-sample processing using Presston™1000 Positive Pressure Manifold.
Fig. 1 Overview of Front
Locator Plate
Holds manifold spacers,
96-well plate products
and is moveable,
for ease of tube or plate
preparation and cleaning
Locator Plate Holder
Allows movement of locator
plate to convenient plate
preparation and cleaning
High Pressure Gauge
Displays the high pressure
flow
SPE Procedure Indicator
Track the SPE steps to
stay on track
High Pressure Regulator
Used for very viscous sample
processing or to dry the
SPE plate
Flow Path Switcher
Move between low and
high flow
Manifold Shield
Holds the manifold in place and
pneumatically moves up and down
Low Pressure Gauge
Displays the low pressure
flow
Gas Flow Meter
Fine tune low pressure
adjustment
Low Pressure Regulator
Used for load, condition,
equilibrate, wash, elution
steps of SPE
Manifold Positioning
Up and Down Switches
Place both hands on the switches to move
the manifold shield up and down
Presston 1000 is compatible with standard 96-well plates and collection plates, including
microelution plates and can even be used with a tabless tube holder (Phenomenex Part
No.: AH0-9055) for tabless 1 mL tubes. Simply change the manifold spacers to fit correctly.
5
Phenomenex
l
WEB: www.phenomenex.com
Gas Regulator with
Filter (equipped with
gas regulating valve
and pressure gauge)
Ensures that the maximum
pressure capacity is not met
Fig. 2 Overview of Back
Inlet from Gas Source
Gas Out to
Manifold Shield
Manifold Spacer
Manifold Shield
Gas In from
Gas Regulator
6
Instructions
Phenomenex
l
WEB: www.phenomenex.com
Need additional accessories?
Contact your technical consultant for any requests.
Accessories
Screwdriver, Screws, and Gas Regulator
Waste Collection Trough
Manifold Spacer Small (2x)
Manifold Spacer Large (1x)
Tubing (6 mm)
Converter Kits
to accommodate different sizes of tubing.
Straight Diameter Joint (6 mm - 4 mm)
Straight Diameter Joint (8 mm - 6 mm)
Straight Diameter Joint (10 mm - 6 mm)
Bent Diameter Joint Regulator Valve
Quick-Fitting Straight Joint Plug C
Quick-Fitting Straight Joint Plug D
Barbed to Male Tube Fitting (¼ in.)
Tubing (10 mm)
All items below are required for set-up.
Starter Kit includes:
2nd
1st 3rd
7
Phenomenex
l
WEB: www.phenomenex.com
Set-Up
Unpacking
1. Open the instrument package carefully by removing the molded foam around the unit and removing
the accessories and converter kits.
2. Remove the manifold by lifting from the bottom and place on flat horizontal surface. Do not lift the
instrument by the MANIFOLD SHIELD.
Gas Regulator Installation
1. Take out GAS REGULATOR from the accessories kit and carefully unpack. Turn the Presston 1000
around (Fig. 2).
2. Position the GAS REGULATOR so the clear part is on the bottom and the black knob is facing upwards
and screw into this position (Fig. 3).
3. Take the tubing from the accessories kit which will be cut into 3 pieces to be attached to the GAS
SOURCE, GAS REGULATOR, and to the back of the MANIFOLD SHIELD (Fig. 4).
4. For the 1st piece (longest piece of tubing), insert one side into GAS SOURCE and the other end into
the left side of the GAS REGULATOR. If the gas source inlet tubing size is different than 6 mm, use
converter kit to connect or contact your technical consultant with questions.
5. Proceed to insert the 2nd piece into the right side of the GAS REGULATOR and then proceed to
insert the other end into the “GAS IN” port.
6. For the 3rd piece of tubing, insert into “GAS OUT” port and insert the other end into the
MANIFOLD SHIELD.
7. Additional tubing and converter inlets are provided in the accessories kit for diverse sizes of tubing.
Fig. 3 Gas Regulator Position on back side Fig. 4 Gas Flow Path Set-Up/Tubing
Back Side
Insert screws here
gas source
Gas
Regulator gas in
gas out
23
1
View a Presston 1000 Set-Up Video at www.phenomenex.com/Presstonsetup
8
Instructions
Phenomenex
l
WEB: www.phenomenex.com
Gas Flow Control
1. HIGH PRESSURE REGULATOR is used to dry the SPE well or for very viscous samples and maxes
out at 0.4 MPa (60 psi). Rotating clockwise increases pressure and rotating counterclockwise decreases
pressure. The HIGH PRESSURE GAUGE can be read in MPa, Bar, or psi.
2. LOW PRESSURE REGULATOR adjusts the flow rate of gas during normal sample preparation
steps, such as load, condition, equilibrate, etc. The max pressure output is 0.1 MPa (14.5 psi). Rotating
clockwise increases pressure and rotating counterclockwise decreases pressure. The LOW PRESSURE
GAUGE can be read in MPa, Bar, or psi.
3. The GAS FLOW METER is used as an additional feature in conjunction with the Low Pressure Regulator
to ensure accurate and precise low pressure monitoring. The pressure should be adjusted slowly to
avoid a surge of high pressure. The range of the GAS FLOW METER is from 3-12 L/min and the pressure
increases clockwise and decrease counterclockwise.
Note: Adjust the pressure knobs and flow meter slowly to ensure steady increase or decrease in flow rate. Do not rotate
the regulating knob too fast because this may cause harm to the regulating valve.
LOW FLOW HIGH FLOW
Condition
Load
Wash
Dry
Elute
OFF
UP
DOWN
UP
DOWN
Down Status
377
[14,84]
220,74
[8,69]
301,16
[11,86]
LOW FLOW HIGH FLOW
Condition
Load
Wash
Dry
Elute
OFF
UP
DOWN
UP
DOWN
t
o
i
n
c
r
e
a
s
e
g
a
s
f
l
o
w
,
r
o
t
a
t
e
t
o
t
h
e
r
i
g
h
t
Fig. 5 Gas Control
Regulator Knobs
Set-Up (continued)
To depressurize the system after use,
rotate to the left
9
Phenomenex
l
WEB: www.phenomenex.com
Getting Started
Refer to Figure 6 for images to help with the operation of Presston 1000
1. Ensure that the FLOW PATH SWITCHER is in the off position.
2. Turn on the gas and adjust the pressure from the GAS SOURCE to 0.5 MPa (72 psi) and adjust the black
knob on top of the GAS REGULATOR so that the pressure is between 0.3-0.5 MPa (43-72 psi). If the GAS
REGULATOR shows no change in pressure, continue to turn the black knob until the regulator pathway is
opened. The GAS REGULATOR ensures that the pressure inside the Presston 1000 does not exceed the
pressure capacity of the manifold. Note: If the sample is viscous and needs more pressure, adjust the gas
supply pressure to 0.7 MPa (101 psi) and adjust the GAS REGULATOR to 0.5-0.7 MPa (72-101 psi).
3. Place both hands on the MANIFOLD POSITIONING SWITCHES and move into the UP position. The
MANIFOLD SHIELD will move up. Note: both switches must be flipped up at the same time and the gas
source must be flowing in order to move the manifold shield (Figure 6.1).
4. Once the MANIFOLD SHIELD has stopped moving, pull out the LOCATOR PLATE and set the
appropriate number of manifold spacers (1 or 2 depending on the height of 96-well plates and waste
trough) onto the locator plate.
Set the waste container trough on top of the manifold spacer(s) and then
proceed to place the 96-well plate that will be used for the extraction on top of the waste collection trough
(Figure 6.2).
5. Add samples or solvents into the wells of the plate (Figure 6.3) and push the locator plate back under the
MANIFOLD SHIELD (Figure 6.4).
6. Place both hands on the MANIFOLD POSITIONING SWITCHES and move into the DOWN position.
The MANIFOLD SHIELD will move down. Note: both switches must be flipped down at the same time
and the gas source must be flowing in order to move the manifold shield.
7. Turn the FLOW PATH SWITCHER to either LOW PRESSURE REGULATOR or HIGH-PRESSURE
REGULATOR (Figure 6.5) and adjust to the desired pressure (Figure 6.6). Note: Use low flow rate for
condition, equilibrate, load, wash, dry, and elution steps; high flow rate for dry down steps and very
viscous samples.
8. If neccessary, use the GAS FLOW METER as a fine tune flow adjustment feature and slowly adjust to the
proper pressure (Figure 6.7).
9. Once liquid has cleared the sorbent, proceed to turn the FLOW PATH SWITCHER into the OFF position
and repeat steps 3-8 for the next step in the SPE protocol.
10. Before the elution step, take out the waste container and replace with a collection plate and proceed to
start with steps 5-8 again (Figure 6.8).
11. Don’t forget to switch the SPE PROCEDURE INDICATOR after performing each step.
12. When extractions are complete, ensure that the system is depressurized by turning the
GAS REGULATOR VALVES to the left (counterclockwise).
Operation
10
Instructions
Phenomenex
l
WEB: www.phenomenex.com
Fig. 6 Operation Instructions for Presston 1000
Adjust the flow to
either low pressure
or high pressure
depending on the
step.
Use both hands to move
manifold shield up or
down.
Adjust the pressure
regulator knobs to
the desired pressure.
Place the 96-well
plate product on top
of the waste trough or
collection plate, which
sits on the manifold
spacers.
If necessary, slowly
adjust the gas flow
meter.
Load sample or solvents
into the wells that will be
processed.
Before elution step,
remove waste trough
and replace with a
collection plate.
Slide the locator plate
under the manifold shield
for processing. Move the
manifold shield down.
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
11
Phenomenex
l
WEB: www.phenomenex.com
Gas Purification
A gas source is required for Presston 1000 Positive Pressure Manifold and no additional power source is
required. Nitrogen or compressed gases are usually used as the gas supply, but laboratories need to ensure
that the gas is filtered, industrial sourced, and free of moisture. Not using the correct gas source will damage
Presston 1000 and result in a loss of warranty.
Note: Changing 4 µm filter in the filtering regulating valve each year is recommended.
Cleaning
Clean the Presston 1000 regularly to ensure that the device is in good working condition. Use a soft cotton
cloth to gently wipe down the machine when cleaning and do not wipe the front or back panel with an organic
solvent. If any samples or solvent are spattered or spilled on the device, clean immediately to avoid the
corrosion to the surface of the device. Clean the top gasket in between extractions with an IPA solution to
ensure that no contamination occurs.
Gasket Replacement
The gasket needs to be replaced when the seal between the manifold shield and the 96-well plate product is
not tight anymore. See page 12 for more information on changing the gaskets.
Manifold Shield Positioning
Turn down the manifold shield when the experiment is completed or not in use. Do not leave the manifold
shield in the UP position long term.
Maintenance
Please visit www.phenomenex.com/Presstonwarranty for complete warranty information.
Warranty Information
Phenomenex warrants the Presston 1000 will be free of defects
in materials and workmanship under normal installation, use, and
maintenance for a period of 12 months following delivery.
12
Instructions
Phenomenex
l
WEB: www.phenomenex.com
1. If any leaking or irregular noise is occurring from the Presston 1000, remove the sealing plates from
the manifold shield. Starting with the top seal plate, A, (pictured below) check that the seal is attached
correctly, if this seems to be the problem, tighten the seal plate A. If the O-ring seems to be aging, replace
the O-ring.
2. After checking seal plate A, if the same issues occur, check seal plate B. Check that the seal is attached
correctly, if this seems to be the problem, tighten the seal plate B. If the O-ring seems to be aging, replace
the O-ring.
3. Replace the gasket when the seal between the manifold shield and the 96-well plate product is not
properly functioning. Replacement gaskets can be purchased from Phenomenex. Peel the old gasket
away, starting from the corner and discard. Clean off any excess adhesive from the manifold using
methanol. Locate the replacement gasket and peel away the adhesive backing on the new gasket, starting
from the corner. Discard the backing material. Align the new gasket with the four corners of the manifold
before attaching the gasket with the adhesive side facing down.
Tips
For other troubleshooting issues or to order replacement O-rings or gaskets, talk with a live technical expert.
www.phenomenex.com/chat
Fig. 7
Fig. 8
Fig. 9
13
Phenomenex
l
WEB: www.phenomenex.com
Frequently Asked Questions
Will I need to purchase a warranty?
A 12 month warranty is provided to you free of charge starting when Presston is received in the lab, please
view all Presston 1000 terms and conditions on www.phenomenex.com/presstonwarranty.
If there is a problem and I need to fix Presston, what should I do?
to make sure your Presston 1000 gets xed and might be able to send you a replacement while you wait.
What products are compatible with Presston 1000?
All common 96-well plate sample preparation products are able to be used with Presston 1000, even
microelution formats. With the addition of a tabless tube holder (Phenomenex Part No.: AH0-9055) 1 mL
tabless tubes can be processed with Presston 1000. To t correctly on the positive pressure manifold, use a
manifold spacer, either small (2x) or large (1x).
To learn more about sample preparation products that Phenomenex
supplies, go to www.phenomenex.com/sampleprep
Fig. 7
Fig. 8
Fig. 9
Phospholipid Removal +
Protein Precipitation
Solid Phase
Extraction
Filtration
QuEChERS
simplifiedliquid extraction
• • •
• • • •
• • • •
• • • • • • •
• • • • • • • •
Supported
Liquid
Extraction
Solid
Phase
Extraction
Proteinaceous samples require a protein precipitation step to promote protein
aggregation which allows their removal from the solution/sample.
Supported Liquid Extraction (SLE) is a faster, easier, andmore reliableway to perform
liquid-liquid extraction. Unwanted interferences can be removed such as proteins, salts
and phospholipids.
simplified liquid extraction
Supported Liquid Extraction
A separation process that is used to remove compounds from a mixture, based on their
physical and chemical properties. Analytical laboratories use solid phase extraction to
concentrate and purify samples for analysis from a wide variety of matrices.
Phospholipid
Removal
+
Protein
Precipitation
Biological samples require the removal of endogenous phospholipids and proteins
as they are a primary source of ion suppression and resulting matrix effects.
β-Glucuronidase
Removal
Protein
Precipitation
A β-Glucuronidase enzyme removal method to clean-up hydrolyzed urine
from samples in less than 1 minute, ideal for rapid drug testing.
Polymeric SPEPolymeric SPE
Solid Phase Extraction
β-Glucuronidase Removal Products
PhospholipidRemovalSolutions
Specifically Extract Target
Analyte
Solvent Switching
De-salt
Remove Phospholipids
Remove Proteins
Remove Particulates
Increase Column Lifetime
Concentrate
Advantages
Technique
• • • • • • • •
Isolate RNA and DNA-based therapeutics / metabolites from biological fluids and tissues in 15 minutes
with no liquid-liquid extraction and an optimized method for LC-MS bioanalysis.
DNA/RNA
Extraction
www.phenomenex.com
Phenomenex products are available worldwide. For the distributor in your country,
GU65451218_W
Terms and Conditions
Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.
Trademarks
Strata and Clarity are registered trademarks, and Novum, Phree, Impact, β-Gone, and Presston are trademarks of Phenomenex.
Strata-X is patented by Phenomenex. U.S. Patent No. 7,119,145
Novum is patent pending.
FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures.
© 2019 Phenomenex, Inc. All rights reserved.
Positive Pressure Manifold User Guide
Australia
t: +61 (0)2-9428-6444
Austria
t: +43 (0)1-319-1301
Belgium
t: +32 (0)2 503 4015 (French)
t: +32 (0)2 511 8666 (Dutch)
Canada
t: +1 (800) 543-3681
China
t: +86 400-606-8099
Denmark
t: +45 4824 8048
Finland
t: +358 (0)9 4789 0063
France
t: +33 (0)1 30 09 21 10
Germany
t: +49 (0)6021-58830-0
India
t: +91 (0)40-3012 2400
Ireland
t: +353 (0)1 247 5405
Italy
t: +39 051 6327511
Luxembourg
t: +31 (0)30-2418700
Mexico
t: 01-800-844-5226
The Netherlands
t: +31 (0)30-2418700
New Zealand
t: +64 (0)9-4780951
Norway
t: +47 810 02 005
Portugal
t: +351 221 450 488
Singapore
t: +65 800-852-3944
Spain
t: +34 91-413-8613
Sweden
t: +46 (0)8 611 6950
Switzerland
t: +41 61 692 20 20
Taiwan
t: +886 (0) 0801-49-1246
United Kingdom
t: +44 (0)1625-501367
USA
t: +1 (310) 212-0555
All other countries
Corporate Office USA
t: +1 (310) 212-0555
Table of contents
