Sartorius Microsart Series User manual

1000061444
Operating Instructions
Original Operating Instructions
Microsart®Manifold
Vacuum Filtration System for the Microbiological Quality Control of Liquid Samples
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Operating Instructions Microsart®Manifold 3
Contents
Contents
1 About these Instructions.....................6
1.1 Scope..................................6
1.2 Accompanying Documents ................7
1.3 Target Groups...........................7
1.4 Symbols Used ...........................8
1.4.1 Warnings in Operating Instructions ..8
1.4.2 Other Symbols....................8
2 Safety Precautions ..........................9
2.1 Intended Use............................9
2.2 Personnel Qualification...................9
2.3 Significance of these Instructions .........10
2.4 Proper Working Order of the Product ......10
2.5 Action in the Event of an Emergency ......10
2.6 Accessories, Consumables, and Spare Parts..11
3 Product Description . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Product Overview.......................12
3.1.1 Structure of 1-way|3-way|6-way
Manifold .......................13
3.1.2 Structure of 2-way Round Arch
Manifold .......................14
3.1.3 Base Support (for Manifold and
Round Arch Manifold) ............15
3.2 Filtration through the Vacuum ...........15
4 Installation................................16
4.1 Scope of Delivery .......................16
4.1.1 Microsart®Manifolds for Microsart®
Funnels and Microsart®@filter ....16
4.1.2 Microsart®Manifolds for
Biosart®100 Monitors ............16
4.1.3 Microsart®Manifolds for
Biosart®250 Funnel ..............17
4.1.4 Microsart®Manifolds for 100 ml
Stainless-steel Funnel ............17
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4 Operating Instructions Microsart®Manifold
Contents
4.1.5 Microsart®Manifolds for 500 ml
Stainless-steel Funnel ............18
4.2 Selecting an Installation Site .............19
4.3 Unpacking.............................20
4.4 Installing the Product ...................20
5 Getting Started............................21
5.1 Integration of the Microsart®Manifold
in the Process Structure .................21
6 Operation .................................22
6.1 Preparing the Process ...................22
6.1.1 Preparing the Base Support
(When Using Microsart®@filters) ..22
6.1.2 Preparing the Base Support
(When Using Biosart®100) ........22
6.1.3 Preparing the Base Support
(When Using Stainless-Steel
Funnels, the Microsart®Funnel
and Biosart®250) ................23
6.2 Starting the Process.....................23
6.3 Finishing the Process ....................24
6.4 Repositioning the Process Structure .......24
6.4.1 Fitting the Cover Cap.............24
6.4.2 Removing the Cover Cap ..........25
6.4.3 Attaching the Hose and
Hose Coupling...................25
6.4.4 Removing the Hose ..............25
6.4.5 Connecting Two Manifolds ........26
6.4.6 Disconnecting Two Manifolds......26
6.4.7 Removing the Base Support .......27
6.4.8 Attaching the Base Support .......27
7 Cleaning and Maintenance..................28
7.1 Cleaning Product Surfaces ...............28
7.2 Maintenance Schedule ..................28
7.3 Cleaning the Manifold...................29
7.3.1 Cleaning after each Filtration: .....29
7.3.2 Cleaning at the End of the
Work Day.......................29
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Operating Instructions Microsart®Manifold 5
Contents
7.4 Replacing the O-Rings...................30
7.5 Greasing the Valve Stopcock .............30
7.6 Autoclaving Microsart®Manifold (Body)
and Base Support ......................32
7.7 Replacing the Silicone Feet...............32
8 Malfunctions ..............................33
9 Decommissioning ..........................35
10 Transport .................................35
11 Storage and Shipping ......................36
11.1 Storage ...............................36
11.2 Returning the Product and Parts ..........36
12 Disposal...................................37
12.1 Information on Decontamination .........37
13 Technical Data.............................38
13.1 Dimensions and Weights.................38
13.2 Connections and Hoses ..................38
13.3 Permitted Operating Pressure.............39
13.4 Sterilization ...........................39
13.5 Ambient Conditions at the
Installation Site ........................40
13.6 Materials..............................40
13.7 Approved Cleaning Agents ...............41
14 Sartorius Service...........................42
15 Accessories, Consumables, and Spare Parts ...43
15.1 Accessories ............................43
15.2 Consumables...........................43
15.2.1 Microsart®Funnels . . . . . . . . . . . . . . . 43
15.2.2 Microsart®@filter, 100 ml ........44
15.2.3 Microsart®@filter, 250 ml ........45
15.2.4 Biosart®100 Monitors ............46
15.2.5 Biosart®250 Funnels .............47
15.2.6 Membrane Filters ................47
15.3 Spare Parts ............................48
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6 Operating Instructions Microsart®Manifold
About these Instructions
1 About these Instructions
1.1 Scope
These instructions are part of the product. These instructions apply to the product in
the following versions:
Product for Microsart®funnel and Microsart®@filter units Model
1-way Microsart®manifold 168M1-MS
2-way Microsart®round arch manifold 168M2-MS
3-way Microsart®manifold 168M3-MS
6-way Microsart®manifold 168M6-MS
Product for Biosart®100 monitors Model
1-way Microsart®manifold 168M1-BS100
2-way Microsart®round arch manifold 168M2-BS100
3-way Microsart®manifold 168M3-BS100
6-way Microsart®manifold 168M6-BS100
Product for Biosart®250 funnel Model
1-way Microsart®manifold 168M1-BS250
3-way Microsart®manifold 168M3-BS250
6-way Microsart®manifold 168M6-BS250
Product with 100 ml stainless-steel funnels Model
1-way Microsart®manifold 168M1-SS100
3-way Microsart®manifold 168M3-SS100
6-way Microsart®manifold 168M6-SS100
Product with 500 ml stainless-steel funnels Model
1-way Microsart®manifold 168M1-SS500
3-way Microsart®manifold 168M3-SS500
6-way Microsart®manifold with 500 ml stainless-steel funnels 168M6-SS500
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Operating Instructions Microsart®Manifold 7
About these Instructions
1.2 Accompanying Documents
t In addition to these instructions, observe the following documentation:
− Operating instructions
Microsart®e.jet Transfer Pump
1.3 Target Groups
These instructions are addressed to the following target groups. The target groups
must possess the specified knowledge.
Target group Knowledge and responsibilities
User The user is familiar with the operation of the product
and the associated work processes. They understand the
hazards which may arise when working with the product
and know how to prevent them.
The user has been trained in the operation of the product.
Laboratory manager The laboratory manager makes decisions about the use and
configuration of the product.
Operator The product operator is responsible for compliance with
safety requirements and workplace safety regulations.
The operator must ensure that anyone working with the
product has access to the relevant information and is
trained to work with the product.
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8 Operating Instructions Microsart®Manifold
About these Instructions
1.4 Symbols Used
1.4.1 Warnings in Operating Instructions
WARNING
Denotes a danger with the risk that death or severe injury may result if it is not
avoided.
CAUTION
Denotes a hazard that may result in moderate or minor injury if it is not avoided.
NOTICE
Denotes a danger with the risk that property damage may result if it is not avoided.
1.4.2 Other Symbols
t Required action: Describes actions which must be carried out.
y Result: Describes the result of the actions carried out.
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Operating Instructions Microsart®Manifold 9
Safety Precautions
2 Safety Precautions
2.1 Intended Use
The product is a manifold. A vacuum filtration system is formed by connecting the
manifold to a pump.
You can use this vacuum filtration system in combination with the corresponding
membrane filters to inspect a wide range of liquids for microbiological contamination.
The manifold is thus a key component in microbiological quality control for liquid
samples.
Operating Conditions for the Product
The product may only be used indoors. The product may only be used with the
equipment and under the operating conditions described in the Technical Data
section of these instructions.
The product must be operated with negative pressure. The vacuum source to generate
the negative pressure must be suitable for the product (for suitability, see Chapter
“13.2 Connections and Hoses”, page 38).
Modifications to the Product
You must not modify or repair the product or make any technical changes. Any
retrofitting or technical changes to the product are only permitted with prior written
permission from Sartorius.
2.2 Personnel Qualification
If persons who do not possess sufficient knowledge about safely handling the product
are working on the product, those people can injure themselves or others.
t Ensure that all persons working on the product possess the necessary skills and
qualifications (for a description, see Chapter “1.3 Target Groups”, page 7).
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10 Operating Instructions Microsart®Manifold
Safety Precautions
t If a particular qualification is required for the tasks described, these tasks must be
performed by the required target group.
t If no qualification is required for the tasks described, the tasks can be performed
by the target group “User”.
2.3 Significance of these Instructions
Failure to follow the instructions in this manual can have serious consequences,
e.g. exposure of individuals to electrical, mechanical, or chemical hazards.
t Read the instructions carefully and in full.
t Ensure that the information contained in these instructions is available to all
individuals working with the product.
t Keep the instructions safe.
t If these instructions are lost: Request a replacement or download the latest version
from the Sartorius website (www.sartorius.com).
2.4 Proper Working Order of the Product
A damaged product or worn parts may lead to malfunctions or cause hazards which
are difficult to recognize.
t Only operate the product when it is safe and in perfect working order.
t Observe the maintenance and cleaning intervals (for intervals and maintenance
tasks, see Chapter “7 Cleaning and Maintenance”, page 28).
t Have any malfunctions or damage repaired immediately by Sartorius Service.
2.5 Action in the Event of an Emergency
If there is immediate danger of personal injury or equipment damage, e.g., due to
malfunctions or dangerous situations, the product must be immediately taken out of
operation.
t Shut down the vacuum pump.
t Set the valve stopcock to “Stop”.
t In the event of a malfunction, proceed as per the malfunction table (see Chapter
“8 Malfunctions”, page 33).
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Operating Instructions Microsart®Manifold 11
Safety Precautions
2.6 Accessories, Consumables, and Spare Parts
The use of unsuitable accessories, consumables, and spare parts can affect the
functionality and safety of the device and have the following consequences:
− Risk of injury to persons
− Damage to the product
− Malfunctioning of the product
− Failure of the product
− Incorrect test results
− Loss of the sample to be tested
− Contamination of the sample to be tested
t Only use approved accessories, consumables, and spare parts supplied by Sartorius.
t Only use accessories, consumables, and spare parts that are in proper working
order (see Chapter “15 Accessories, Consumables, and Spare Parts”, page 43).
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12 Operating Instructions Microsart®Manifold
Product Description
3 Product Description
3.1 Product Overview
Fig.1: Microsart®manifolds
51 3 42
6
7
9
8
No. Designation
1 6-way manifold
2 3-way manifold
3 Base support with sintered frit
4 1-way manifold
No. Designation
5 2-way round arch manifold
6 Serial number
7 Distribution number
8 “Stop” position
9 “Start” position
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Operating Instructions Microsart®Manifold 13
Product Description
3.1.1 Structure of 1-way|3-way|6-way Manifold
Fig.2: Body of 1-way manifold (example)
2
3
4
5
7
1
6
No. Designation
1 Cover cap
2 Knurled screw with flat spring
3 Ventilation hole
No. Designation
4 Hose with quick-lock coupling
5 Valve stopcock
6 Body of 1-way manifold
7 Silicone foot
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14 Operating Instructions Microsart®Manifold
Product Description
3.1.2 Structure of 2-way Round Arch Manifold
Fig.3: Body of 2-way round arch manifold
2
6
4
1
5
3
No. Designation
1 Knurled screw with flat spring
2 Ventilation hole
3 Valve stopcock
4 Connector for adaptation to the
Microsart®e.jet pump
No. Designation
5 Body of 2-way round arch
manifold
6 Silicone foot with adjustable
stainless-steel housing
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Operating Instructions Microsart®Manifold 15
Product Description
3.1.3 Base Support (for Manifold and Round Arch Manifold)
Fig.4: Base support
1
2
3
No. Designation
1 Frit
2 Base support
(168M1-MS – 189M3-MS)
No. Designation
3 Base support
(168M1-BS100 – 168M6-BS250)
(168M1-BS100 – 168M6-SS500)
3.2 Filtration through the Vacuum
The method of membrane filtration enables you to determine the microbiological
quality of liquid samples.
By means of filtration, any microorganisms present in the sample are concentrated on
the membrane filter in order to then cultivate them on a culture medium.
Reusable stainless-steel funnels and various disposable products can be used on the
product in conjunction with a filter membrane. A membrane filter and a funnel with a
specific volume must be placed on the suction strip in advance for filtration. In order
to filter the liquid sample through the filter, the manifold must first be connected to
a vacuum source (Sartorius recommends using a Microsart®e.jet vacuum pump).
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16 Operating Instructions Microsart®Manifold
Installation
4 Installation
4.1 Scope of Delivery
4.1.1 Microsart®Manifolds for Microsart®Funnels and Microsart®@filter
Manifolds
1-way 2-way 3-way 6-way
Manifold 1 1 1 1
Base support for 47 mm filter 1 2 3 6
Sintered frit 1 2 3 6
Vent filter 1 2 3 6
Cover cap to seal the output 1 1 1
Quick coupling for adapting to the
Microsart®e.jet vacuum pump
1
Silicone hose with quick-lock coupling, 20 cm 1 1 1
Operating instructions 1 1 1 1
4.1.2 Microsart®Manifolds for Biosart®100 Monitors
Manifolds
1-way 2-way 3-way 6-way
Manifold 1 1 1 1
Base support for 47 mm filter 1 2 3 6
Sintered frit 1 2 3 6
Biosart®adapter 1 2 3 6
Vent filter 1 2 3 6
Cover cap to seal the output 1 1 1
Quick coupling for adapting to the
Microsart®e.jet – vacuum pump
1
Silicone hose with quick-lock coupling, 20 cm 1 1 1
Operating instructions 1 1 1 1
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Operating Instructions Microsart®Manifold 17
Installation
4.1.3 Microsart®Manifolds for Biosart®250 Funnel
Manifolds
1-way 3-way 6-way
Manifold 1 1 1
Base support for 47 mm and 50 mm filters 1 3 6
Sintered frit 1 3 6
Silicone seal 1 3 6
Plug to seal the ventilation opening 1 3 6
Cover cap to seal the output 1 1 1
Silicone hose with quick-lock coupling, 20 cm 1 1 1
Operating instructions 1 1 1
4.1.4 Microsart®Manifolds for 100 ml Stainless-steel Funnel
Manifolds
1-way 3-way 6-way
Manifold 1 1 1
Base support for 47 mm and 50 mm filters 1 3 6
Sintered frit 1 3 6
Silicone seal 1 3 6
100 ml stainless-steel funnel 1 3 6
Cover (for 100 ml) 1 3 6
Seal (for 100 ml) 1 3 6
Plug to seal the ventilation opening 1 3 6
Cover cap to seal the output 1 1 1
Silicone hose with quick-lock coupling, 20 cm 1 1 1
Operating instructions 1 1 1
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18 Operating Instructions Microsart®Manifold
Installation
4.1.5 Microsart®Manifolds for 500 ml Stainless-steel Funnel
Manifolds
1-way 3-way 6-way
Manifold 1 1 1
Base support for 47 mm and 50 mm filters 1 3 6
Sintered frit 1 3 6
Silicone seal 1 3 6
500 ml stainless-steel funnel 1 3 6
Cover (for 500 ml) 1 3 6
Seal (for 500 ml) 1 3 6
Plug to seal the ventilation opening 1 3 6
Cover cap to seal the output 1 1 1
Silicone hose with quick-lock coupling, 20 cm 1 1 1
Operating instructions 1 1 1
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Operating Instructions Microsart®Manifold 19
Installation
4.2 Selecting an Installation Site
t Make sure that the following conditions are met at the installation site:
Condition Features
Ambient conditions − Suitability tested (see Chapter “13.5 Ambient
Conditions at the Installation Site”, page 40 for
ambient conditions)
Setup surface − Stable and even surface, as well as sufficient
operating height
− Sufficient surface area for the product and the
peripheral devices (for the product space
requirements, see Chapter “13.1 Dimensions and
Weights”, page 38; for peripheral device space
requirements, see instructions for the peripheral
devices, see Chapter “1.2 Accompanying
Documents”, page 7)
− Access to a vacuum source and a drain is provided
Access to parts relevant to
operation
Convenient and safe
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20 Operating Instructions Microsart®Manifold
Installation
4.3 Unpacking
Procedure
t Unpack the product.
t If the product is stored temporarily: Observe the
storage information (see Chapter “11.1 Storage”,
page 36).
t Install the product at the intended installation
location.
t Keep all parts of the original packaging, e.g. to
return the product.
4.4 Installing the Product
Procedure
t Install the following components (as needed) on
the ventilation hole (2):
− The sterile ventilation filter (without touching
the filter opening)
Or:
− The sealing plug
t Connect the product to the vacuum source via the
hose (1).
t Turn the valve stopcock to “Stop”.
t Ensure that the filtrate is being properly drained on
the pump side.
t Ensure that the sealing cap (3) is fitted.
1 32
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