Photon Systems Instruments FMT150 User manual

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CONTENTS
Contents.................................................................................................................................................................. 2
Instruction Manual.................................................................................................................................................. 4
Warnings and Safety Precautions ........................................................................................................................... 4
General Description ................................................................................................................................................ 5
Device Description .................................................................................................................................................. 6
List of different Photobioreactor FMT150 types ............................................................................................... 8
List of parameters measured and/or calculated by the Photobioreactor FMT150......................................... 10
Components of Photobioreactor FMT150....................................................................................................... 11
Description of the Photobioreactor Control Unit Front Panel ........................................................................ 20
Description of the PBR Rear Panel for version FMT150.1 ............................................................................... 21
Description of the PBR Rear Panel for version FMT150.2 ............................................................................... 23
Installation ............................................................................................................................................................ 26
Device Installation ........................................................................................................................................... 26
Accessories/optional components........................................................................................................................ 34
O2electrode installation.................................................................................................................................. 34
CO2electrode installation................................................................................................................................ 35
Magnetic stirrer installation ............................................................................................................................ 37
External light panel installation....................................................................................................................... 39
High-precission GAS Mixing system ................................................................................................................ 40
Peristaltic pump installation............................................................................................................................ 43
Gas analysis system GAS 150........................................................................................................................... 46
PWM Pump...................................................................................................................................................... 50
Photobioreactor software..................................................................................................................................... 51
Software Installation and Use ......................................................................................................................... 51
Graphical User Interface (GUI) ........................................................................................................................ 51
User´s Guide for cultivation of algae and cyanobacteria ...................................................................................... 62
Preparation and set up of the components .................................................................................................... 62
Preparation of the device and Setting up of the experiment.......................................................................... 62
Appendix 1 –Installation of the Operating System .............................................................................................. 64
Appendix 2 –Control Menu Tree.......................................................................................................................... 66
Explanation of Control Keys and Used Symbols .............................................................................................. 67
Graphical Presentation of the Operation Schematic....................................................................................... 68
Appendix 3 –Detailed Explanation of the Pulse Light Control ............................................................................. 74

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Appendix 4 –O2Electrode Calibration.................................................................................................................. 75
Appendix 5 –CO2Electrode Calibration................................................................................................................ 76
Appendix 6 –Conversion table for CO2calibration............................................................................................... 82
Appendix 7 –GAS 150 Technical specifications .................................................................................................... 83
Appendix 8 –QY measurement ............................................................................................................................ 84
Appendix 9 –Description and vessel assembly of 3000 ml vessel........................................................................ 85
Technical Specification Standard 400ml PBR ........................................................................................................ 86
Technical Specification Standard 1000ml PBR ...................................................................................................... 87
Technical Specification Standard 3000ml PBR ...................................................................................................... 88
WARRANTY TERMS AND CONDITIONS.................................................................................................................. 89
Alphabetical Index................................................................................................................................................. 90
List of Abbreviations ............................................................................................................................................. 92
Troubleshooting and Customer support............................................................................................................... 93

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INSTRUCTION MANUAL
WARNINGS AND SAFETY PRECAUTIONS
PLEASE READ THE FOLLOWING INSTRUCTIONS CAREFULLY BEFORE TURNING THE PHOTOBIOREACTOR ON:
Remove all packaging and transport protectors before connecting the photobioreactor to the power
supply.
Use only cables supplied by the manufacturer.
Keep the device dry outside and avoid working in high humidity environment!
The manufacturer is not responsible for any damage due to improper operation!
Water and other liquids should only be placed in vessels designed for the purpose and according to
instructions included in this manual.
GENERAL ELECTRICAL SAFETY GUIDELINES:
Perform a routine check of the devices and their wiring.
Replace worn or damaged cords immediately.
Use appropriate electrical extension cords/power bars and do not overload them.
Place the device on a flat and firm surface. Keep away from wet floors and counters.
Avoid touching the device, socket outlets or switches if your hands are wet.
Do not perform any alterations to the electrical parts of the device or its components.
WARNING:
The Photobioreactor is considered Class 1M* LED Product. LED radiation may be harmful to eye. Avoid
direct and strongly reflected exposure. Use protective glasses.
*Class 1M: Laser and LED equipment that is safe for the naked eye under foreseeable conditions of
operation. Looking directly into the source of radiation by employing optics within the beam, such as
magnifying glass, telescope or microscope, can be potentially hazardous.

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GENERAL DESCRIPTION
Photobioreactor FMT 150 feature a unique combination of the cultivator and computer controlled monitoring
device. The Photobioreactor FMT150 combines a cultivation vessel with the built-in fluorometer and
densitometer. It is primarily designed for modular and precise phototrophic cultivation of algae, cyanobacteria
and bacteria. Light power and spectral composition as well as temperature and aeration gas composition can
be set with high accuracy.
User defined cultivation conditions can be dynamically varied according to user - defined protocol. Continuous
online monitoring of cultivation conditions and measured parameters is possible via the optional
Photobioreactor Software and a PC unit. Light, temperature, gas composition, and culture medium regime can
be programmed to oscillate with various amplitudes and frequencies with user-defined time steps from
milliseconds to hours via the software or via the control unit. Continuous-flow turbidostatic cultivation can be
used for the stabilization of the suspensions via optical density control. In addition, up to 8 peristaltic pumps
for different chemostat or pH-stat cultivation control can be linked to the Photobioreactor FMT150 for the
highly precise control of cultivation conditions.
The growth of the cultures is monitored by the integrated densitometer (OD 720, 680 nm). Relative chlorophyll
content of the culture can also be monitored continuously by the difference of optical densities at 680 and 720
nm. The instantaneous physiological state of the culture is measured by the Photosystem II quantum yield
Fv/Fm.
The Photobioreactor FMT150 has a flat-vessel design that enables uniform illumination over the whole culture.
Photobioreactors are currently manufactured in five versions differing in the volume of their cultivation
vessels: 400 ml, 1000 ml and 3000 ml, 25 L and 120 L.
All Photobioreactor models may be supplied with a number of optional accessories - sensors, stirrers, pumps,
etc. that can be all controlled via Photobioreactor Software. Control of the Photobioreactor is possible with
either computer and the software or via the control unit of the photobioreactor itself. However the data can
only be logged and collected with the software and the computer.

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DEVICE DESCRIPTION
Standard version of the Photobioreactor FMT150 consists of a cultivation vessel with a sealable lid and a base
box containing electronics circuitry, LED light panel (Fig. 1A) and other components essential for the optimal
operation of the photobioreactor.
The cultivation vessel is flat and rectangular in shape with a volume capacity of 400 ml, 1000 ml or 3000 ml. Its
front and back windows are made of glass plates. The base is made out of stainless steel and contains a
thermal bridge that facilitates heat transfer between a Peltier cell in the instrument base and the culture
suspension. The lid of the vessel is easily detachable during cleaning and contains Luer connectors for gas or
medium supply tubing, waste medium exhaust or sample collection. The three larger ports accommodate the
optional temperature/pH sensor and dissolved O2or CO2 probes.
Fig. 1 A) Photobioreactor body consists of a flat vessel holding the suspension and of a base box with electronics circuitry. B)
The block scheme of the instrument shows the Control Processor Unit exchanging data and protocol with external
computer (PC) and auxiliary external modules. The Control Unit governs measurements of parameters such as OD and
chlorophyll fluorescence plus parameters measured by auxiliary sensors. It also controls the actinic light sources and
temperature of the culture. C) Detail of the LED configuration. D) Uncorrected optical density spectrum of cyanobacterial
suspension. The lines with arrows indicate wavelengths of various light sources that are integrated in the instrument. The
detection range of the photodiode is also shown. E) Detail (rotated top-to-bottom view) of the fluorometer and

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densitometer. F) Schematic representation of optional gas supply components and modules that can be used to stabilize
aeration and set CO2concentration level.
The array of high-power light emitting diodes (LEDs) is located behind the culture vessel. These LEDs generate a
highly uniform irradiance flux that can be controlled in the range 0-3000 mol (photons)/m-/s1PAR. The
irradiance can be dynamically modulated by the instrument control unit or by an external computer with
software. In the bottom corner of the front of the vessel is a semiconductor light sensor for measuring
fluorescence emission and suspension optical density by attenuation of light that was emitted from the LEDs
(Fig. 1E).
The Photobioreactor can also control external modules such as peristaltic pumps and solenoid valves via the
photobioreactor control unit (shown schematically in Fig. 1F and Fig. 2). The solenoid valves are used to switch
off the gas supply to the culture (bubbling) during optical measurements. Peristaltic pumps are used in
turbidostat and chemostat modes to supply fresh medium or buffer.
The Photobioreactor can be connected to an optional, external PC computer that runs photobioreactor
software. The photobioreactor software can define and control experimental settings (therefore overriding the
photobioreactor controller): duration of the experiment (typically days), data collection cycle (typically
minutes), temperature or temperature dynamics, LED irradiance, light/dark cycle or other irradiance dynamics.
The external computer with photobioreactor software is necessary for collection and logging of all the
measured parameters from both the photobioreactor and the auxiliary sensors. These parameters are
displayed in real time graphs. If the PC computer with photobioreactor software is hooked up to the internet,
the data, as well as control of the growth conditions can be accessed remotely online from anywhere. During
remote access data can be downloaded and growth conditions can be changed. One PC with photobioreactor
software can control up to 7 photobioreactors with additional software licence per additional photobioreactor.
Fig. 2 Schematic diagram of the Photobioreactor, its modules, and remote control.

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LIST OF DIFFERENT PHOTOBIOREACTOR FMT150 TYPES
The Photobioreactor is currently manufactured in three types enabling the customer to select the optimal
system with respect to volume. Various options for light quality, light intensity and thermoregulation are also
available. Numerous additional accessories such as various sensors can be supplied as described below on page
14.
Please note that this manual describes operation and use of two available PBR FMT150 versions (Fig. 3), PBR
FMT150.1 and new PBR FMT150.2 version released in June 2013.
CULTIVATION VESSEL CAPACITY:
400 ml (in FMT150/400)
1000 ml (in FMT150/1000)
3000 ml (in FMT150/3000)
LED LIGHTNING:
Two standard LED panel versions are available to select form, each colour is controlled independently:
- White-Red panel: maximum intensity 1,500 µmol(photon)/m2/s (white 750 / red 750 µmol(photon)/m2/s)
- Blue-Red panel: maximum intensity 1,500 µmol(photon)/m2/s (blue 750 / red 750 µmol(photon)/m2/s)
Two optional upgraded LED panel versions are available, each colour is controlled independently:
- White-Red panel: maximum intensity 3,000 µmol(photon)/m2/s (white 1500 / red 1500 µmol(photon)/m2/s)
- Blue-Red panel: maximum intensity 3,000 µmol(photon)/m2/s (blue 1500 / red 1500 µmol(photon)/m2/s)
Optional customized panel versions:
- White-Blue: maximum intensity 1,500 µmol(photon)/m2/s
- White-Blue with Light Upgrade: maximum intensity 3,000 µmol(photon)/m2.s
- Red: maximum intensity 750 µmol(photon)/m2/s (no light upgrade available)
- White: maximum intensity 1,500 µmol(photon)/m2/s
- White with Light Upgrade: maximum intensity 3,000 µmol(photon)/m2/s
- Blue: maximum intensity 1,500 µmol(photon)/m2/s
- Blue with Light Upgrade: maximum intensity 3,000 µmol(photon)/m2/s

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TEMPERATURE CONTROL:
Requires presence of the pH/Temperature probe.
- Standard range of 18 –50 °C (static or ramping).
In table below please find approximate estimation of time required for the given temperature equilibration.
Initial
temperature
Target
temperature
Light conditions
µmol/m2/s
Equilibration time
required
5 °C
50 °C
1500
2.5 hours
50 °C
5 °C
1500
3 hours
- Optional enhanced range 0-70 °C (static or ramping) (requires enhanced vessel made from nickel-plated brass
covered with ceramic layer).
VERSIONS:
FMT150.1 –PBR FMT150/400, PBR FMT150/1000
FMT150.2 - PBR FMT150/400, PBR FMT150/1000, PBR FMT150/3000
IMPORTANT: The standard version of FMT150 in both 400 and 1000 ml models has recently (June 2013) been
upgraded from FMT150.1 (Fig. 3A) to FMT150.2 (Fig. 3B). This manual provides instructions for operation of
both versions (original, now referred to FMT150.1 and the current versions FMT150.2. When following this
manual please ensure that you refer to the correct instructions.
Fig. 3 Two versions of the PBR FMT 150 available. A) FMT 150.1, B) FMT 150.2.

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LIST OF PARAMETERS MEASURED AND/OR CALCULATED BY THE PHOTOBIOREACTOR FMT150
Symbol
Name
Description
Ft (Dark)
Instantaneous fluorescence in
the dark
Instantaneous F-yield in dark equals F0
Ft = F0 (in dark-adapted sample)
Ft (Light)
Instantaneous fluorescence in
the light
Instantaneous F-yield in light that results
from a dynamic equilibrium of plastoquinone
reducing and re-oxidizing processes and from
non-photochemical quenching
Ft = Fs(in light-adapted sample)
FM (Dark)
Maximum fluorescence in
dark-adapted state
Maximum F-yield with QAreduced (qP=0) and
non-photochemical quenching relaxed
(NPQ=0)
FM (Light)
Maximum fluorescence in
light-adapted state
Maximum F-yield with QAreduced (qP=0) and
non-photochemical quenching active (NPQ≠0)
FV
Maximum variable
fluorescence
FV = FM - F0
QY(Dark)
Instantaneous PSII quantum
yield in the dark
Effective PSII quantum yield
QY= FV / FM(dark-adapted sample)
QY (Light)
Instantaneous PSII quantum
yield in the light
Effective PSII quantum yield relaxing in light
QY= FV’ / FM’(light-adapted sample)
OD680
Optical density at 680 nm
Optical density representing light scattering
and chlorophyll absorption.
OD720
Optical density at 720 nm
Optical density representing light scattering.
pH
pH
pH is the negative logarithm of the hydrogen
ion concentration in the medium.
[O2]
Oxygen concentration
Concentration of dissolved oxygen.
[CO2]
Carbon dioxide concentration
Concentration of dissolved carbon dioxide.
CO2partial pressure is calculated using the
measured pH and the temperature.

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COMPONENTS OF PHOTOBIOREACTOR FMT150
Photobioreactor FMT150 can be purchased in different configurations from minimal configuration up to
complex system consisting of numerous accesory units linked together and controlled via the Photobioreactor
control unit itself for most of the optional accesories or by Remote Control System for PBR FMT150 for full
operation of the system.
Minimal required configuration that is recommended to be purchased for optimal operation of the
Photobioreactor cultivation and monitoring system consists of set of standard photobioreactor components
(as listed below) plus air pump with bubble interruption valve and pH/temperature module. Components that
are part of minimal Photobioreactor FMT150 configuration are shown in Fig. 5. Optional components that are
available as accesory modules and can be purchased together with Photobioreactor FMT150 are described in
next section on page 14.
Photobioreactor FMT150 purchased in minimal configuration can be effectively used for cultivation and
monitoring purposes, however without purchasing Remote Control System for PBR FMT150 no measured data
can be stored and visualised as well as no user-defined protocols can be designed. Explanation on page 20 and
Menu Tree schematics on page 66 describe how to operate the PBR FMT150 in cultivation mode via front panel
control unit, without Remote Control System for PBR FMT150 respectively.
Note: Customers are kindly asked to check the contents of the delivered package and compare it with enclosed
package list generated based on customer‘s order. Please contact the [e-mail] if any discrepancies
occur.
SET OF STANDARD PHOTOBIOREACTOR FMT150 COMPONENTS
As shown on pg. 13 and Fig. 5
1. Power supply with the power cord.
2. Photobioreactor with integrated double-modulation fluorometer- Ft, Fm, QY and integrated optical
density measurement.
3. Cultivation vessel with capacity 400 ml (with holder) with FMT150/400, 1000 ml with FMT150/1000 or
3000 ml with FMT150/3000.
4. LED panel shield.
5. Spare Part Kit*–for the complete list of Spare Part Kit see Table 1 on page 12 and corresponding Fig. 4
6. Instruction Manual.
7. Bubble Humidifier - To maintain stable culture volume.
Components #8 and 9 are highly recommended for proper functioning of the photobioreactor but are not
included in the standard configuration and have to be purchased separately.
8. Bubble interruption valve with air pump (air pump not shown on in Fig. 5).
9. pH/temperature module.

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Fig. 4 Components of the spare part kit. Numbers correspond to component number in table 1.
Table 1: List of spare part kit components shown in Fig. 4.
Component
Number
Component Description
Specification
Explanation
Pcs
1
Male Luer Integral Lock MTLL240-J1A*
5/32” (4.0 mm)
10
2
Female Luer Thread Style BSFTLL-J1A*
5/16” Hex to 1/4-28 UNF
Bottom Sealing Thread
10
3
Male Luer Integral Lock Ring Plug LP4-J1A*
10
4
Tee Tube Fitting T40-J1A*
5/32” (4.0 mm)
10
5
Reduction Tube Fitting 4060-1*
1/4" (6.4 mm) and 5/32” (4.0
mm)
5
6
Double Check Valve DCV118-001*
Safety valves that permit
gases and liquids to flow in
only one direction
5
7
Check Valve SCV08053*
Check valve for with low
priming volume.
5
8
Metal lid with ports (8a) with silicone ring (8b)
Silicon ring 27x42x2 mm
For humidifier bottle
2+2
9
O-ring
75x3 mm
Sealing between lid and the
vessel
5
10
O-ring
14x2 mm
Sealing to the openings for
optional sensors
10
11
Silicone Tubing
Ø 6/3 mm
Additional tubing
10 m
12
Silicone Tubing
Ø 5/2 mm
Additional tubing
0.5 m
13
Hex Key
5 mm
To loosen up the screws of
the lid (Fig.17 3a, 3b)
1
14
Spanner/Screwdriver
8 mm
For releasing filler screws in
optional sensor positions
and luer connectors in the
vessel lid
1
15
T-tube (silicone tubing with 2 pcs of Male Luer
Lock and 1 pc of Tee Tube Fitting)
Aeration tubing for the
vessel
1
16
Wrench
19 mm
For releasing sensors
1
* Provided by: www.valueplastics.com
1
2
3
5
4
6
7
9
10
11
12
13
14
15
8a
8b
8a
8b
9
10
11
12
13
14
16
15
Version FMT 150.1 Version FMT 150.2

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Fig. 5 Minimal configuration set of the Photobioreactor FMT150. Numbers correspond to numbers of components as
described on page 11 and 14.

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LIST OF OPTIONAL PHOTOBIOREACTOR FMT150 COMPONENTS
8. Air pump with bubble Interruption valve - Air pump for culture bubble stirring and supply of air
levels of CO2. Valve interrupts bubbling during measurements, required for precise readings of OD.
Bubble interruption valve can be purchased also without air pump if required.
9. pH/Temperature module - To measure pH and temperature. It is required for temperature control
of photobioreactor. The module consists of electronic unit built-in Photobioreactor FMT150 Control
Unit, sensor probe and probe cable (Fig. 6). InPro3253SG/120/PT1000 or InPro3253/120/PT1000
single rod measuring cells from METTLER TOLEDO (www.mt.com) are supplied as low maintenance,
autoclavable and pressure resistant pH electrodes with a factory filled pre-pressurised liquid
electrolyte and a reference electrolyte together with a silver ions barrier. Measurement disturbances
caused by earth potentials are avoided through employment of a “solution ground” option available
with InPro3250SG version. For InPro3250 version please refer to description of grounding during
calibration on page 31. For more information please refer to manual provided by electrode
manufacturer.
Fig. 6 pH/temperature module.
10. O2Module - To measure concentration of dissolved O2. The module consists of electronic unit built-in
Photobioreactor FMT150 Control Unit, sensor probe and probe cable (Fig. 7).dO2sensor
InPro6800/12/120 from METTLER TOLEDO (www.mt.com) with integrated temperature probe is
used for oxygen measurement. Operation of the InPro6800/12/120 sensor is based on polarographic
measurement. For more information please refer to manual provided by electrode manufacturer.
Please note that Photobioreactor control software records dO2measurements in mV. Calibration of
the dO2probe has to be performed outside of the photobioreactor software and then applied
externally (in a spreadsheet software) to the raw data collected by the software for conversion to mg
O2/L or %.

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Fig. 7 O2Module
11. CO2Module - To determine the dissolved carbon dioxide concentration in the medium. The module
consists of external electronic unit for connection with Photobioreactor FMT150 Control Unit, sensor
probe and probe cable (Fig. 8). InPro5000/12/120 sensor from METTLER TOLEDO (www.mt.com)
with integrated temperature probe is used for potentiometric CO2concentration measurement. The
InPro5000 sensor employs a gas permeable silicone membrane which is tightly stretched around a
special engineered flat pH membrane. CO2partial pressure can be calculated using the pH and the
temperature. The CO2probe needs to be calibrated ouside of the photobioreactor software and
calculated calibration coefficients can be entered in the software for automatic conversion of the
raw mV data to units of mg/L. For more information please refer to manual provided by electrode
manufacturer and calibration instructions included in this manual in appendix 5.
Fig. 8 CO2Module
12. Magnetic stirrer - The magnetic stirrer (Fig. 9) rotates a teflon-coated magnetic bar in a vertical
plane of the front glass panel inside the Photobioreactor. The frequency of the rotation is adjustable
in the software. The stirrer is essential when the agitation with gas bubbles cannot be used, e.g.,
when the photosynthetic oxygen evolution or respiratory oxygen uptake are measured, in anaerobic
culture. It is also essential when dO2probe or CO2probes are used with the photobioreactor as

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consistent stirring around the probes is essential to accurate measurements of dO2and dCO2. It can
be also used to help reduce cell sedimentation or clumping when used together with gas bubbling.
Note: Magnetic stirrer cannot be used simultaneously with an external light panel as they both fit on
the outside of the vessel.
Fig. 9 Magnetic stirrer. A) FMT 150.1 version; B) FMT 150.2 version.
13. PWM Pump (for Gas or Liquid) - PWM pump (Fig. 10) can be used for pumping liquid or gas into the
cultivation cuvette; it can be regulated in software within a range of 10-100 % of total output. The
pump has diverse uses: (1) Controlled air bubbling in the cultivation vessel; (2) Single gas input into
the vessel; (3) When connected to the vessel inlet and outlet, it can be used for suspension mixing
(bubbling) in anaerobic conditions. Please note that PWM pump cannot be used simultaneously with
magnetic stirrer as both need to be connected to AUX 1 on the back of the photobioreactor.
Fig. 10 PWM Pump
14. High-Precision Gas Mixing System - Gas Mixing System GMS 150 (Fig. 11) can produce precise
mixtures of up to 4 different gasses. The flows of the individual input gases are measured by thermal
mass flow meters and adjusted by integrated mass flow controllers. Before the exit connector, the
gas mixture is thoroughly homogenized. The input and output gas connectors are of Prestolok type
allowing fast and secure connection to a variety of tubes. High-precision gas mixer can have up to
four channels. The standard version of the system includes two channels: Channel 1 supplies Air or
N2; Channel 2 supplies CO2. Please note that when one GMS is used to supply gas to more than one
A. B.

17
photobioreactor the use of one PWM pump per photobioreactor is required to supply portion of the
gas from the gas mixing system to the bioreactor without affection the gas flow between the
bioreactors.
Fig. 11 GMS 150.
15. Gas Analysis System GAS 150 - Enables measurements of CO2 gas in the head space of the
cultivation vessel as it leaves via the vessel gas output (Fig. 12).
Fig. 12 GAS 150.
16. Peristaltic Pump PP500 –Peristaltic pump PP500 (Fig. 13) is used for continuous-flow turbidostatic
cultivation. These pumps can be operated in turbidostat or chemostat mode. In turbidostat mode
when Turbidostat Module I. is purchased continuous-flow turbidostatic cultivation is enabled. In the
most basic setup, the Photobioreactor optical sensing unit monitors the optical density (OD680,
OD720) of the culture. The Peristaltic Pump adds medium according to user set OD level to keep the
culture turbidity constant. In chemostat mode the pH value of the cultivation media can be
continuously adjusted as either acid or base is added to the cultivation vessel to keep the pH level at
user preset level. The pump allows variable flow-rate range from 10 to 5600 µl/min.

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Fig. 13 Peristaltic Pump PP500.
17. Remote Control System for PBR FMT150 - Remote Control System for PBR (Fig. 14) includes a PC
with photobioreactor software for data collection, data visualization and remote control of
photobioreactor. The software operates in Linux operating system and allows set up of user-defined
protocols; Data collection in real time; Data upload for processing even when the experiment is
running (in Excel accessible format); Data visualization in graphs; Remote control via internet.
Supplied with the PC computer and the software is USB cable for PC and PBR FMT150
communication (Please note that in older versions of FMT150 serial cable with USB adapter are
included).
Fig. 14 Remote Control System for PBR FMT150. A) Version FMT 150.1; B) Version FMT 150.2.
18. Additional Software License - A separate license is required for each additional Photobioreactor
software control. One PC with photobioreactor software can control up to 7 photobioreactors.
19. Light Upgrade - Increases intensity of actinic light in the Photobioreactor. The maximum reachable
depends on light colour as listed on pg. 8.
20. External Light Panel –Can be used to accommodate specific needs of different organisms or
increases illumination intensity. External light panel is offered in three versions: single colour, single
colour with additional IR LEDs, and bicolour (see page 8 for detail list of available versions). Please
note that the external LED panel cannot be used simultaneously with the magnetic stirrer.
21. Supplementary pH/T Probe - Additional sensor probe to pH/Temperature Module.
22. Additional Cable to Probes - Supplementary cable and connector for O2or pH/T measuring probe.

19
23. Supplementary O2Probe–Additional sensor probe to O2module.
24. O2Membrane Kit - O2membrane replacement kit for O2probe. Includes: 4 membrane bodies, 1 O-
ring set, 25 ml electrolyte.
25. CO2Membrane Kit - CO2membrane replacement kit for CO2probe. Includes: 4 membrane bodies, 1
O-ring set, 25 ml electrolyte.
26. Supplementary Vessel (standard model) –additional vessel, suitable for cultivation in the range of
18 –50 °C. Made from stainless steel and glass, it includes the frame, lid and glass body.
Manufactured in a 400 ml, 1000 ml or 3000 ml version.
27. Enhanced Vessel - Suitable for cultivation in the range of 0 - 70 °C. Made from nickel-plated brass
covered with ceramic layer. Manufactured in a 400 ml, 1000 ml or 3000 ml version.
28. Replacement Glass Window for Cultivation Vessel - Includes only the glass body (not the frame,
base, or lid). Made of boiling glassware, thickness 3.3 mm. Delivered for 400 ml, 1000 ml or 3000 ml
vessels.
29. Gasket Kit for Cultivation Vessel - Includes: 5 pcs Silicone gasket for vessel base, 5 pcs Silicone
gasket for vessel lid, 15 pcs Silicone O-ring 75/3, 15 pcs Silicone O-ring 16/2. Delivered for 400 ml,
1000 ml or 3000 ml vessels.

20
DESCRIPTION OF THE PHOTOBIOREACTOR CONTROL UNIT FRONT PANEL
Photobioreactor Control Unit front panel can be used for simple manual control of the PBR FMT150. By using
the keys on the front panel the operation of cultivation and monitoring units such as pH/temperature sensor,
light control, OD monitoring module, PWM modeor CO2and O2sensor can be controlled. When FMT150
photobioreactor is purchased without the Remote Control System for PBR FMT150 then all of the control has
to be set up manually using the front panel of the FMT150 control unit. The user can read out from the display
the actual values for the given sensor or monitoring unit.
IMPORTANT: Please note that no user-defined protocols can be designed and no data collection is possible
without the PC and software.
NOTE: Please note that neither turbidostat nor chemostat module can be operated if FMT150 is purchased
without the Remote Control System for PBR FMT150.
Remote Control System for PBR FMT150 is used for controlling all the functions of the FMT150 Photobioreactor
with the exception of pH probe calibration step that needs to be done via front panel of the control unit. The
instructions for the pH probe calibration are described in “process of pH calibration”on page 31. The
calibration has to be done using the FMT150 control unit and the buttons on the front panel of the unit.
The front panel of the FMT150 control unit has 4 LED lights on the left and 5 control keys on the right (Fig. 15).
Fig. 15 Photobioreactor controller front panel.
1. LED INDICATORS
Green LED indicator READY is lighting when the current temperature is equal to target temperature.
Orange LED indicator HEAT is lighting when the heater turns on in the cultivation vessel.
Blue LED indicator COOL is lighting when the water in the cultivation vessel is cooled and the Cooling Unit is
operating. Note: The minimal temperature in the standard PBR FMT150 is 10 °C.
Red LED indicator OVERLOAD is lighting when the requested temperature in the cultivation vessel higher than
the current temperature was not reached yet.
2. DIGITAL DISPLAY ON THE LEFT
Displays the current temperature in the cultivation vessel and error messages if any problems with the FMT150
occur.
Possible error messages are: