pyroscience AquapHOx User manual

Version V1.13 www.pyroscience.com
AquapHOx® Logger
Underwater O2, pH, T Meter
MANUAL
O2
pH
T

AquapHOx® Logger | Manual
© PyroScience GmbH 2
AquapHOx® Logger
Underwater O2, pH, T Meter
Document Version 1.13
The AquapHOx® Loggers are released by:
PyroScience GmbH
Kackertstrasse 11
52072 Aachen
Germany
Phone [phone]
Fax [phone]
Email [e-mail]
Web www.pyroscience.com
Registered: Aachen HRB 17329, Germany

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TABLE OF CONTENT
1Introduction............................................................................................................................................... 4
2Overview.................................................................................................................................................... 5
2.1 Optical Port...................................................................................................................................... 5
2.2 Built-In Temperature Sensor ........................................................................................................... 5
2.3 Air Pressure Compensation............................................................................................................. 6
2.4 Included Accessories ....................................................................................................................... 6
2.5 Optional Accessories ....................................................................................................................... 7
3Software Installation.................................................................................................................................. 9
4Configuration........................................................................................................................................... 10
4.1 Connecting the Sensor Head ......................................................................................................... 10
4.2 Sensor Settings .............................................................................................................................. 11
4.3 Calibration Setup........................................................................................................................... 11
4.4 Calibration of Oxygen Sensors....................................................................................................... 11
4.5 Calibration of pH Sensors .............................................................................................................. 12
4.6 Calibration of Optical Temperature Sensors................................................................................. 12
5Measurements......................................................................................................................................... 14
5.1 Online Measurements................................................................................................................... 14
5.2 Data Logging.................................................................................................................................. 14
5.3 Charging the Battery...................................................................................................................... 15
5.4 Deployment................................................................................................................................... 15
5.5 Data Retrieval................................................................................................................................ 16
5.6 Compensation of pH Sensor Drift.................................................................................................. 17
6Appendix.................................................................................................................................................. 18
6.1 Dimensions.................................................................................................................................... 18
6.2 Device Specification....................................................................................................................... 18
6.3 Warnings & Safety Guidelines ....................................................................................................... 19

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1INTRODUCTION
The AquapHOx® Logger family (item no. APHOX-LX, APHOX-L-PH, APHOX-L-O2) are long-term loggers for
underwater operation down to 4000 m (APHOX-LX) or 100 m (APHOX-L-PH and APHOX-L-O2) depth. The
AquapHOx® Logger platform offers one optical port for connection of a wide range of optical O2- or pH-
sensors from PyroScience. A fixed NTC temperature sensor provides automatic temperature compensation
of the optical sensors. The integrated rechargeable LiPo battery offers stand-alone logging for up to 1 year.
A huge internal data memory can store up to 40 million data points. The included USB cable is used for
operating the device with the user-friendly software Pyro Workbench.

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2OVERVIEW
The front of the AquapHOx® Logger provides the port for connecting optical sensors using the PyroScience
SUB-connector, the fixed NTC temperature sensor for automatic temperature compensation, and a
mounting thread for calibration containers or protective cage during deployment/transport. The backside
of the device provides the connector with the USB interface.
The housing is dependent on the chosen device. APHOX-LX comes with a titanium housing for deployments
down to 4000m. APHOX-L-PH and APHOX-L-O2come with a POM housing capable for deployments down to
100m.
2.1 Optical Port
The AquapHOx® Loggers are compatible with PyroScience sensors for underwater applications designated
by the appendix ‘-SUB’ in the item number. Any of these sensor heads can be connected to the SUB-
connector (“optical port”) of the AquapHOx® Logger. The shallow-water versions APHOX-L-O2and APHOX-
L-PH only support sensor heads for either oxygen or pH, respectively. The deep-sea version APHOX-LX
supports oxygen as well as pH sensor heads for e.g. successive measurements.
2.2 Built-In Temperature Sensor
Optical oxygen and pH sensors show generally a temperature dependency, which can be automatically
compensated by the device. For this purpose, the AquapHOx® Logger offers a high-precision, fast
responding NTC temperature sensor. This sensor is fixed to the housing, it cannot be removed.

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2.3 Air Pressure Compensation
For APHOX devices, the Pyro Workbench offers two ways to compensate for deviating ambient air pressure
at the water surface. The ambient air pressure can either be entered in "hPa" as fixed pressure (measured
at the water surface), or in approx. meters above sea level.
2.4 Included Accessories
1x USB configuration cable: allows easy calibration and setup with the powerful Windows software
packages Pyro Workbench or Pyro DeveloperTool, as well as charging of the battery. Not intended for
deployment during sampling.
1x Dummy Plug: used for protecting the electrical connector from water during the deployment.
1x Protection Cage: provides protection of the sensor ports during deployment or transport.
2x Calibration Containers: used for calibration standards during sensor calibration.
Calibration Capsules: used for preparation of calibration standards.

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2.5 Optional Accessories
Sensor protection cage with anti-(bio)fouling net (item: APHOX®-CAGE-AF).
Anti-(bio)fouling net for underwater (-SUB) pH and oxygen sensor caps (item APHOX®-CAP-AF).
For optimal protection, the anti-(bio)fouling net (copper alloy) for the sensor cap protection cage (item:
APHOX®-CAP-AF) should be combined with the sensor protection cage for AquapHOx® meters (item:
APHOX®-CAGE-AF). Both protection cages can be purchased separately at any time and replaced once
exhausted.

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Flow-through-cell (item: APHOX®-FTC).
The flow-through cell allows to connect the APHOX® devices to a flow path. It can be used fully submerged,
as well as in the laboratory, and can be combined with all PyroScience cap sensors with SUB-connector.
Typical use cases are pumped CTD systems, FerryBox Systems, and industrial applications. Please refer to
the APHOX®-FTC leaflet for product mounting.

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3SOFTWARE INSTALLATION
The AquapHOx® Logger is operated with the user-friendly software Pyro Workbench. This software offers
comfortable settings and calibration wizards, as well as advanced logging features. Several devices can be
operated in parallel within a single window.
System requirements: PC with Windows 7/8/10/11 and min. 1000 MB free disk space.
Do not connect the USB cable to your PC before the software has been installed! The software will
automatically install the appropriate USB-drivers.
Installation steps:
•Download the Pyro Workbench from https://www.pyroscience.com/en/downloads/underwater-
devices
•Unzip and start the installer and follow the instructions.
•Connect the AquapHOx® Logger to your PC with the included USB cable.
•Start the Pyro Workbench software.
For more detailed information on the software, please refer to the Pyro Workbench manual available at
https://www.pyroscience.com/en/downloads/underwater-devices.

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4CONFIGURATION
This chapter provides short instructions how to install, configure, and calibrate sensor heads to the
AquapHOx® Logger.
For in-depth information especially on sensor settings and sensor calibration, please refer to the respective
Analyte Sensor Manual for optical oxygen or pH sensors available at
https://www.pyroscience.com/en/downloads/underwater-devices.
4.1 Connecting the Sensor Head
An index matching liquid inside the
connector enhances the sensor signals.
Before connecting a sensor, insert the
provided Pasteur pipette filled with
deionized water (NOT seawater) to the
bottom of the connector and fill it
completely with the deionized water
(NOT seawater).
Alternatively, and mandatory for the
optical fiber with lens (item no. SPFIB-
LNS-SUB/SPFIB-LNS-CL2-SUB) for read-
out of sensor spots, insert the provided
Pasteur pipette filled with silicone oil
to the bottom of the connector and fill
it with silicone oil.
The sensor is inserted and secured
with the cap nut. Do not use a wrench.
It is sufficient to tighten the nut by
hand.

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4.2 Sensor Settings
Open the settings Window in Pyro Workbench. Enter the Sensor Code of the connected sensor and adjust
all further required settings as requested by the respective software.
4.3 Calibration Setup
Similar to the settings wizard, the user is guided through a calibration wizard for
performing the required optical sensor calibration.
When using sensor caps, calibration can be performed by screwing the included
calibration containers onto the AquapHOx® device.
When using fiber-based sensors, please ensure that the temperature sensor of the
AquapHOx® has the same temperature as your calibration solution.
4.4 Calibration of Oxygen Sensors
The sensor needs to be calibrated in air-saturated water or in air with 100% relative
humidity (upper calibration) and optionally in de-oxygenated water (0%
calibration).
1-point calibration
•For calibration in air saturated water, it is very important that the water is indeed 100% saturated
with air. Fill an appropriate amount of demineralized water into a flask. Stream air through the water
with an air stone connected to an air pump (available as commercial equipment for fish aquaria) for
about 10 minutes.
•When using the calibration container, it is possible to put wet paper tissues or a sponge onto the
bottom of the calibration container and screw it on the AquapHOx® device to perform a calibration
at air with 100% relative humidity.
•Enter all required parameters in the software, and perform a sensor calibration.
2-point calibration
•For 0% calibration, use the 0% calibration capsules from PyroScience, giving 50mL 0% calibration
standard (item no.: OXCAL).
•For calibration using the calibration container, please dissolve 2 capsules in 100ml deion. water
(NOT seawater). Screw the calibration container onto the device and perform the 0% calibration.

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4.5 Calibration of pH Sensors
•A one-point calibration is obligatory to start measurements.
•A two-point calibration is highly recommended before every measurement.
•An additional pH offset-adjustment is recommended only for advanced applications, e.g.
measurements in complex media.
If the sensor was previously stored under dry conditions or used for the first time, wait for at least 60 min
to achieve wetting of the sensor membrane. This is the minimum time. For higher accuracy, it is necessary
to condition the sensor for several hours (overnight) in e.g., seawater.
1-point calibration
•Screw the calibration container onto the device (in case of using a PHCAP) and let the sensor
equilibrate (at least 15 min) in the stirred pH 2.0 buffer solution (freshly prepared using item no.
PHCAL2). For more information see the pH sensor manual.
•Calibrate the pH sensor.
Important: The pH sensor must stay wet all the time between calibration and measurement. For this purpose,
you might keep the calibration container attached to the device.
2-point calibration
•Perform the 1-point calibration as described above.
•Immerse the pH sensor and the temperature sensor into the stirred pH 11 buffer solution (freshly
prepared using item no. PHCAL11). For more information see the pH sensor manual.
•Calibrate the pH sensor.
pH offset adjustment
The pH offset adjustment does NOT replace the required calibration steps as described above. It offers an
additional pH-offset adjustment using a buffer solution with exactly known pH value. This can be used if a
reference measurement (e.g. spectrophotometric measurement) is available. The pH of this sample needs
to be at the PK value of your sensor (pH 7.5 –8.5 in case of a PK8 sensor). Please refer to the pH sensor
manual for more information.
4.6 Calibration of Optical Temperature Sensors
The shallow-water AquapHOx® devices, item no. APHOX-L-O2and APHOX-L-PH, are capable of using
PyroScience optical temperature fiber sensors. Calibration of these sensors can be performed by using the
internal temperature sensor.
•Click on Calibrate and follow the calibration instructions.

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•Select the desired calibration against the integrated NTC Temperature Sensor or against a Fixed
Reference Temperature.
•Calibrate the optical temperature sensor.
Important: When using the integrated NTC temperature sensor for the reference temperature, ensure that
the connected optical temperature sensors is exposed to the same temperature as the NTC temperature
sensor.

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5MEASUREMENTS
After the device has been configured as described in the previous chapter, measurements and data logging
can be started using the software Pyro Workbench.
5.1 Online Measurements
The main window of Pyro Workbench will automatically start with periodic measurements (indicated by "II")
after a successful sensor calibration. These measurements are not yet saved, they are only visualized in the
software window. The actual readings are shown in tables and graphs. Configure the graphs by clicking on
the graph symbol in the respective row of the table. Please refer to the Workbench Manual for further
instructions. To ensure communication free of interference a connection to a PC should only be established
in electromagnetically controlled environments.
5.2 Data Logging
For data logging, click the red record button in the
upper left corner of the Pyro Workbench.
There are two different options for data logging:
PC Logging and Stand-alone Logging.
Note, both logging modes can be activated in
parallel. However, for most usage scenarios this
does not make sense.
PC Logging requires a permanent connection via the USB cable to the PC. The data are not stored on the
device, but on the PC.

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Stand-alone Logging does not require a connection the PC. The data are stored on the device. After
completion of the experiment, the data are downloaded from the device to a PC.
Important for Stand-alone Logging: Check the battery status. The battery is sufficient for >1-year deployment
with 10 min sampling interval and is fully recharged within ca. 2 hours while connected to a PC.
Enter the required information on the respective logging panel and start the data logging.
5.3 Charging the Battery
The internal LiPo battery is charged by connecting the device to any computer USB 2.0 port or to any standard
USB charger. If the used USB port provides up to 500 mA charging current, the charging is completed within
ca. 2h, if the battery was fully discharged. Some USB ports provide only max. 100 mA, in this case the charging
duration will increase to ca. 10h. The software Pyro Workbench gives you information on the charging status.
5.4 Deployment
If you are using sensors caps (and not fiber-optic sensor heads): Before the actual deployment, it is
recommended to keep the sensor caps immersed in the calibration container filled with the respective
calibration standard. This is especially important for pH sensors in order to keep them wet.
For deployment, disconnect the device from the PC and protect the electrical connector with the dummy
plug. Remove the calibration container shortly before the deployment, and replace it with the protection
cage. Deploy the AquapHOx® Logger.
In shallow water environments, it is strongly recommended to slip over the APHOX®-CAP-AF anti-
(bio)fouling net onto the sensor cap and to use the APHOX®-CAGE-AF with anti-biofouling net on the
APHOX® device (instead the included protection cage without net).
Dummy Plug
Protection Cage

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5.5 Data Retrieval
After retrieving the logger, remove the dummy plug and connect the device to the PC with the included USB
cable.
•Start the Pyro Workbench software.
•Click on Stop Logging
•Download the recorded data to the PC by selecting the respective logfile from the list of Logfiles in
device memory in the Stand-alone logging window and clicking on "Download". These ".txt" files can
be easily imported into common spreadsheet programs.
Data retrieval should only be attempted in electromagnetically controlled environments to ensure
communication free of interference.
Please note that PC and device logging can be simultaneously active. The obtained data via PC logging will
not show up in the device logging file and vice versa.

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5.6 Compensation of pH Sensor Drift
For long-term measurements of pH sensors, it is possible to perform a manual drift compensation using the
Pyro Data Inspector.
•After retrieving the logger, rinse the pH sensor with distilled water and put the sensor into a freshly
prepared pH 2 (PHCAL2) calibration solution. Let the logger record several data.
•Remove the connector dummy and connect the AquapHOx® device to the PC with the included USB
cable. Start the Pyro Workbench software.
•Click on Stop Logging
•Download the recorded data to the PC by selecting the respective logfile from the list of Logfiles in
device memory in the Stand-alone logging window and clicking on "Download and open in Data
Inspector". After the download the Measurement will be opened in the Data inspector.
•For more detailed information on the pH drift compensation and the Data Inspector, please refer to
the Pyro Workbench manual.

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6APPENDIX
6.1 Dimensions
6.2 Device Specification
For sensor specifications and response times, please refer to the webpages of the different sensor types.
Specifications
Item No.
APHOX-LX
APHOX-L-PH / APHOX-L-O2
Dimensions
63 x 300 mm
63 x 300 mm
Weight in air
1.35 kg
0.450 kg
Housing Material
Titanium
Polyoxymethylene (POM)
Max. hydrostatic pressure
400 bar (4000m)
10 bar (100m)
Supported analytes
optical pH and O2
APHOX-L-PH: pH and optical
temperature
APHOX-L-O2: O2and optical
temperature
Optical sensor connector
PyroScience underwater connector (-SUB)

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Optical sensor port
1
Compatible optical sensors
Portfolio of optical sensors with underwater connector (-SUB) from
PyroScience
External temperature sensor
Resolution
Accuracy
Typical response time
NTC thermistor for temperature compensation
0.005°C
0.05°C
0.5 s
Software
Software Pyro Workbench or Pyro DeveloperTool
Computer interface
USB 2.0
Electrical Connector
Subconn MCBH6M
Charging
via included USB cable
Battery
Rechargeable LiPo battery, 1250 mAh
Data storage
4 GB (ca. 40 million data points)
Fastest sample rate
1 Sample per sec.
Typical stand-alone logging time
(fully charged battery)
ca. 1 week for 1s logging interval
ca. 2 months for 10s logging interval
ca. 6 months for 1 min logging interval
ca. 1 year for >10 min logging interval
Operating / storage temperature
-10 –60 °C Storage
-5 –40 °C Operating
Oxygen sensors
Refer to the separately available specifications for the connected
oxygen sensor
pH sensors
Refer to the separately available specifications for the connected
pH sensor
Optical temperature sensors
Refer to the separately available specifications for the connected
optical temperature sensor
*Please note, that the optical sensors have a different temperature range
6.3 Warnings & Safety Guidelines

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In case of problems or damage, disconnect the device and mark it to prevent any further use.
Consult PyroScience for advice! There are no serviceable parts inside the device. Please note that
opening the housing will void the warranty.
Follow appropriate laws and guidelines for safety in the laboratory, like EEC directives for
protective labor legislation, national protective labor legislation, safety regulations for accident
prevention and safety data-sheets from manufacturers of chemicals used during the
measurements and of PyroScience buffer capsules.
Handle the sensors with care especially after removal of the protective cap! Prevent mechanical
stress to the fragile sensing tip! Avoid strong bending of the fiber cable! Prevent injuries with
needle-type sensors!
Handle the protection cages with care to avoid injuries! As the anti-(bio)fouling net contains nickel,
it is strongly recommended to wear gloves during handling, especially in case of a known contact
allergy.
The sensors are not intended for medical or military purposes or any other safety-critical
applications. They must not be used for applications in humans; not for in vivo examination on
humans, not for human-diagnostic or any therapeutic purposes. The sensors must not be brought
in direct contact with foods intended for consumption by humans.
Handle the device with extreme care if there is any suspicion that it was flooded during
deployment. Internal pressure might have built up as a consequence. Point the sensor side of the
device away from people and material assets at all times, and carefully loosen the Subcon
connector to relieve potential internal pressure before sending the instrument to PyroScience for
service.
Keep the sensors and the device out of reach of children!