pyroscience FireSting pro User manual

FireSting®-PRO
Optical Multi-Analyte Meter
USER MANUAL
O2
pH
T
www.pyroscience.com

FireSting®-PRO | User Manual
© PyroScience GmbH 2
FireSting®-PRO
Optical Multi-Analyte Meter
Document Version 1.07
The FireSting®-PRO is released by:
PyroScience GmbH
Hubertusstrasse 35
52064 Aachen
Germany
Phone [phone]
Fax [phone]
Email [e-mail]
Web www.pyroscience.com
Registered: Aachen HRB 17329, Germany

FireSting®-PRO | User Manual
© PyroScience GmbH 3
TABLE OF CONTENT
1Overview..........................................................................................................................................4
2Introduction.....................................................................................................................................5
3Quick Start........................................................................................................................................6
4Connecting Sensors........................................................................................................................7
5Optical Sensors ...............................................................................................................................8
5.1 Fiber-optic Sensors.........................................................................................................................8
5.2 Contactless Sensors........................................................................................................................9
6Extension Port.............................................................................................................................. 11
6.1 Connector X1 (Power, Digital Interface, Analog In)........................................................11
6.2 Connector X2 (Analog Output) ................................................................................................12
7Specifications ............................................................................................................................... 14
8Related Documents..................................................................................................................... 16
9Warnings & Safety Guidelines.................................................................................................. 17

FireSting®-PRO | User Manual
© PyroScience GmbH 4
1OVERVIEW
The compact USB-powered fiber-optic meter FireSting®-PRO with 1, 2, or 4 channels for
multiple analytes and sensor heads features:
•freely configurable channels for O2, pH and temperature
•broad optical sensor portfolio (multiple fiber-based and contactless sensor heads for
several ranges)
•with (ultra-)high speed sampling
•zero-noise and zero-latency temperature compensation
•improved ambient light suppression and
•smart measuring modes for prolonged sensor lifetime
•pH, oxygen and temperature determination simultaneously in one sample
This single-device solution for multi-analyte measurements has integrated atmospheric
pressure and humidity sensors for precise and easy oxygen sensor calibration, but also
for automatic pressure compensation of the oxygen measurements. Furthermore, the
FireSting®-PRO offers 4 analog outputs and a broadcast-mode.
The new innovative and user-friendly Pyro Workbench allows operation of several
FireSting®-PRO meters in parallel as a scalable multi-channel system.

FireSting®-PRO | User Manual
© PyroScience GmbH 5
2INTRODUCTION
The FireSting®-PRO comes with 1, 2, or 4 channels (optical sensor ST-connectors 1to 4)
for up to 4 fiber-optic sensors and one connector (T) for an external Pt100 temperature
probe. The optical channel connectors are color coded, indicating the analyte (oxygen,
pH or temperature), which is currently measured and can be changed for each channel.
The air inlet equilibrates the internal temperature, pressure and humidity sensors with
the surrounding. Avoid covering these holes to ensure free air circulation towards the
internal sensors.
The micro-USB connector on the left side panel provides energy supply and data
exchange with the PC. Right-hand side of it, a connector X1 for power and digital
interface (7-pins) and a connector X2 for analog output (5 pins) is located.

FireSting®-PRO | User Manual
© PyroScience GmbH 6
3QUICK START
Step 1: Download the correct software and manual from the downloads tab of your
purchased device on www.pyroscience.com
Step 2: Connect the FireSting®-PRO meter with the micro-USB cable to the Windows
PC/laptop (Windows 7, 8, 10).
Step 3: Connect appropriate PyroScience sensor(s) to the optical sensor connectors 1to
4at the device (see 4).
Step 4: Connect an external temperature sensor (item no. TDIP15 or TSUB21) to the
Pt100 connector or, alternatively, an optical temperature sensor (see 5) to one of the
optical sensor connectors 1to 4 for automatic temperature compensation.
Step 5: Prepare appropriate calibration standards, as described in the respective sensor
manuals (see 8).
Step 6: Start the logger software by clicking on the short-cut "Pyro Workbench" on your
desktop.
Step 7: Open the Settings Wizard by clicking on the FireSting®-PRO picture. Select the
respective analyte and enter all sensor settings for each sensor, including an appropriate
mode of temperature compensation.
Step 8: Open the Calibration Wizard and follow the calibration instructions for each
sensor. Measurements with the respective sensors will start automatically after all
required sensor calibrations have been performed.
Step 9: Configure the Graphs according to your preferences.
Step 10: Activate Data Logging.

FireSting®-PRO | User Manual
© PyroScience GmbH 7
4CONNECTING SENSORS
The fiber-optic oxygen, pH and temperature sensors, as well as optical fibers needed for
read-out of contactless sensors are connected to the ST-connectors of the FireSting®-
PRO (1to 4) with a male fiber plug.
•Remove the black caps from the plug of the sensor / fiber.
•Remove the red caps from the sensor ports at the FireSting®-PRO (the red caps
should be put on again if it is not in use anymore to protect the optics).
•Insert the male fiber plug of the sensor cable into the ST-port (female fiber
connector) of the FireSting®-PRO and turn the bayonet coupling gently clockwise
until the plug is locked firmly.

FireSting®-PRO | User Manual
© PyroScience GmbH 8
5OPTICAL SENSORS
The FireSting®-PRO is compatible with a broad range of optical oxygen, pH and
temperature sensors from PyroScience.For an overview of available optical sensor
types, please see the PyroScience website.
5.1 Fiber-optic Sensors
Sensor
Item
Analyte
Application
Miniprobes
OXROB…
O2
stirred water, gas
+PHROB …
pH
water & media
Retractable Tip
Minisensors
*OXR…
O2
water, gas & semi-solid
samples
TPR…
Temp.
Fixed Tip
Minisensors
*OXF…
O2
water, gas & semi-solid
samples
(esp. seawater)
TPF…
Temp.
OXF…-PT
O2
gas
(puncturing septa/packaging)
Bare Fiber
Minisensors
OXB…
O2
water, gas & custom
TPB…
Temp.
Solvent-Resistant
Probes
OXSOLV
O2
approved polar and non-polar
solvents
OXSOLV-PTS
O2
approved solvent vapors
*also as Microsensor; + different pH ranges; water=water, seawater, aqueous solutions

FireSting®-PRO | User Manual
© PyroScience GmbH 9
5.2 Contactless Sensors
Sensor
Item
Analyte
Application
Nanoprobes
OXNANO
O2
aqueous solutions &
microfluidics
Sensor Spots
OXSP5
O2
water & gas
TPSP5
Temp.
*PHSP5…
pH
water & media
Sensor Vials
OXVIAL…
O2
water & gas
TOVIAL…
Temp. &O2
+PHVIAL…
pH
water & media
+PHTVIAL…
Temp. &pH
+PHTOVIAL…
Temp. & pH
&O2
Flow-Through Cells
OXFTC…
O2
water & gas
OXFTCR
TPFTC2
Temp.
TOFTC2
Temp. &O2
+PHFTCR…
pH
water & media
+ different pH ranges; water=water, seawater, aqueous solutions

FireSting®-PRO | User Manual
© PyroScience GmbH 10

FireSting®-PRO | User Manual
© PyroScience GmbH 11
6EXTENSION PORT
The extension port of the FireSting®-PRO consists of the two connectors X1 and X2
(fitting connector plugs can be obtained from Phoenix Contact item no. 1778887 and
1778861).
6.1 Connector X1 (Power, Digital Interface, Analog In)
The pin configuration of the connector X1 is given in the table below. Pins 1-2 (GND and
VCC) can be used for providing an external power supply (3.5…5.0 VDC), if the
FireSting®-PRO should not be powered via the USB port. The transmit and receive pins
of the UART-interface are given at pins 4 (TXD) and 5 (RXD) (communication protocol on
request). When using the UART-interface it is recommended to connect pin 3
(/USB_DISABLE) to pin 1 (GND), which disables the USB interface.
Pin 6 (/PAUSE_BROADCAST) and Pin 7 (/TRIGIN) are related to the so called “broadcast
mode” which can be configured in the PC control software (e.g., Pyro Workbench or Pyro
Developer Tool). During broadcast mode, the device triggers itself periodic
measurements which can be read out from the analog outputs or from a text message
transmitted via the USB/UART interface. For more details refer to the manual of the
respective control software are to the communication protocol (available on request).
Pin 7 (/TRIGIN) acts as a trigger input for the broadcast mode. Note, the option “Enable
Trigin” must be enabled in the broadcast settings in the control software. Every time this
pin is connected to pin 1 (GND) then an additional broadcast measurement is triggered.
Pin 6 (/PAUSE_BROADCAST) acts as a master switch of the broadcast mode. As long this
pin is tied to pin 1 (GND), then the broadcast mode is paused. Neither periodic broadcast
measurements, nor triggered broadcast measurements are performed.

FireSting®-PRO | User Manual
© PyroScience GmbH 12
Pin
Name
Function
Description
1
GND
Power
Ground
2
VCC
Power
Power supply,
3.5V to 5.0V DC
max. 70 mA (typ. 40 mA)
3
/USB_DISABLE
Digital Input
Connect to GND for disabling the
USB interface
4
TXD
Digital Output
(UART TX)
UART interface with 3.3V levels (5V
tolerant),
baud rate 115200,
8 data bits, 1 stop bit,
no parity, no handshake
5
RXD
Digital Input
(UART RX)
UART interface with 3.3V levels (5V
tolerant),
baud rate 115200,
8 data bits, 1 stop bit,
no parity, no handshake
6
/PAUSE_
BROADCAST
Digital Input
(0V or 3.3V, internally
pulled-up to 3.3V)
Connect to GND for pausing any
broadcast-mode operation.
7
/TRIGIN
Digital Input
(0V or 3.3V, internally
pulled-up to 3.3V)
Triggers a broadcast measurement
every time, when this pin is tied to
GND.
6.2 Connector X2 (Analog Output)
The connector X2 provides 4 analog outputs with a range of 0-2.5V DC at a resolution of
14 bits (see table below). Refer to the Pyro Workbench manual how to configure the
analog outputs.

FireSting®-PRO | User Manual
© PyroScience GmbH 13
Pin
Name
Function
Description
1
GND
Ground
2
AO_A
Analog Output
(0 –2.5 V DC)
(14-bit resolution)
Analog Output Port A
(alternatively digital
alarm output)
3
AO_B
Analog Output
(0 –2.5 V DC)
(14-bit resolution)
Analog Output Port B
(alternatively digital
alarm output)
4
AO_C
Analog Output
(0 –2.5 V DC)
(14-bit resolution)
Analog Output Port C
(alternatively digital
alarm output)
5
AO_D
Analog Output
(0 –2.5 V DC)
(14-bit resolution)
Analog Output Port D
(alternatively digital
alarm output)

FireSting®-PRO | User Manual
© PyroScience GmbH 14
7SPECIFICATIONS
Feature
Specification
Dimensions
68 x 120 x 22 mm (housing)
78 x 120 x 24 (total)
Weight
ca. 290 g
Interface
USB 2.0
Power supply
USB-powered (max 50mA at 5V)
Supported operating systems
Windows 7, 8, 10
Operating temperature
0 to 50°C
Max. relative humidity
non-condensing conditions
Optical sensor port
1, 2, or 4 (dependent on model)
Optical sensors
complete PyroScience sensor portfolio for O2, pH & T
Optical sensor connector
fiber-optic ST-plug
Max. sample rate
ca. 10 samples per second (depending on Settings)
External temperature port
Range, Resolution, Accuracy*
1 channel for 4-wire Pt100
-30°C to 150°C, 0.02°C, 0.5°C
Internal temperature sensor
Range, Resolution, Accuracy*
-40 to 125°C, 0.01°C, 0.3°C
Internal pressure sensor
Range, Resolution, Accuracy
300 to 1100 mbar, 0.1 mbar, typ. 3 mbar
Internal humidity sensor
Range, Resolution, Accuracy
0 to 100% rel. humidity (RH), 0.04% RH, typ. 0.2% RH
Digital interface
at extension port X1 (7 pins)
UART with 3.3V levels (5V tolerant), 115 200 baud, 8
data bit, 1 stop bit, no parity, no handshake

FireSting®-PRO | User Manual
© PyroScience GmbH 15
Connector plug port X1
Phoenix Contact, item no. 1778887
Analog output (4 channels)
at extension port X2 (5 pins)
0 to 2.5 VDC
14-bit resolution
Connector plug port X2
Phoenix Contact, item no. 1778861

FireSting®-PRO | User Manual
© PyroScience GmbH 16
8RELATED DOCUMENTS
Detailed instructions for using the Pyro Workbench and application of optical oxygen,
pH and temperature sensors:
•manual for logger software "Pyro Workbench" (Windows)
•manuals for optical sensors from PyroScience (oxygen, pH, temperature)

FireSting®-PRO | User Manual
© PyroScience GmbH 17
9WARNINGS & SAFETY GUIDELINES
Before using the FireSting®-PRO and its sensors, read carefully the instructions
and user manuals.
In case of problems or damage, disconnect the instrument and mark it to
prevent any further use! Consult PyroScience for advice! There are no
serviceable parts inside the device. Please note that opening the housing will
void the warranty!
The FireSting®-PRO is not watertight, is sensitive to corrosive conditions and to
changes in temperature causing condensation. Avoid any condition (e.g. direct
sun light) causing a heating of the device above 50°C (122°F) or below 0°C
(32°F). Avoid any elevated humidity causing condensing conditions.
Handle the sensors with care especially after removal of the protective cap!
Prevent mechanical stress to the fragile sensing tip! Prevent injuries with
needle-type sensors!
Calibration and application of the sensors is on the user’s authority, as well as
data acquisition, treatment & publication!
The optical sensors and meter FireSting®-PRO are not intended for medical,
diagnostic, therapeutic, or military purposes or any other safety-critical
applications. The sensors must not be used for applications in humans and must
not be brought in direct contact with foods intended for consumption by
humans.
The FireSting®-PRO and optical sensors should be used in the laboratory by
qualified personnel only, following the user instructions and the safety
guidelines of the manual, as well as the appropriate laws and guidelines for
safety in the laboratory!
Keep the sensors and the fiber-optic meter FireSting®-PRO out of reach of
children!

FireSting®-PRO | User Manual
CONTACT
www.pyroscience.com
PyroScience GmbH
Hubertusstraße 35
52064 Aachen
Deutschland
Tel.: [phone]
Fax: [phone]
[e-mail]
www.pyroscience.com