pyroscience FireStingGO2 User manual

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Pocket Oxygen Meter
FireStingGO2
MANUAL
www.pyroscience.com

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Document Version 1.21
Refers to FireStingGO2Manager software version >1.0
The FireStingGO2is manufactured by
PyroScience GmbH
Hubertusstr. 35
52064 Aachen
Germany
Phone [phone]
Fax [phone]
Email [e-mail]
Internet www.pyroscience.com
Registered: Aachen HRB 17329, Germany

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TABLE OF CONTENT
1INTRODUCTION ..................................................................... 6
2SAFETY GUIDELINES.............................................................. 8
3OVERVIEW FIRESTINGGO2 .................................................... 11
3.1 PORTS AND INTEGRATED SENSORS ............................................. 11
3.1.1 Micro USB Port.................................................................. 12
3.1.2 Oxygen Port ...................................................................... 12
3.1.3 Temperature Port .............................................................. 12
3.1.4 Internal Air Sensor ............................................................. 12
3.1.5 Power Button and Recharging the Battery.......................... 13
3.2 STAND CLAMP......................................................................... 13
3.3 USER INTERFACE...................................................................... 14
3.4 OPERATION MODES ................................................................. 15
3.4.1 Live Mode.......................................................................... 15
3.4.2 Menu Mode ....................................................................... 15
3.4.3 Logging Mode.................................................................... 15
3.4.4 Standby Mode ................................................................... 16
3.4.5 PC Mode ........................................................................... 16
3.5 STATUS LINE ........................................................................... 16
4QUICK START........................................................................18
4.1 GETTING STARTED ................................................................... 18
4.2 SETTINGS AND OPTIONS ADJUSTMENTS ...................................... 18
4.3 SENSOR CALIBRATION .............................................................. 18
4.4 LIVE MEASUREMENTS............................................................... 19
4.5 DATA LOGGING........................................................................ 19
4.6 DATA MANAGEMENT AND INSPECTION......................................... 19
5LIVE MODE............................................................................20
5.1 TOP SCREEN ........................................................................... 20
5.2 SUB-SCREEN 1 ......................................................................... 20
5.3 SUB-SCREEN 2......................................................................... 20
5.4 SUB-SCREEN 3 ......................................................................... 20

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6MENU MODE .........................................................................21
6.1 MAIN MENU............................................................................ 21
6.2 SETTINGS MENU ...................................................................... 21
6.2.1 Sensor Code ...................................................................... 21
6.2.2 Medium............................................................................. 22
6.2.3 Oxygen Units..................................................................... 22
6.2.4 Temperature...................................................................... 22
6.2.5 Salinity.............................................................................. 23
6.3 CALIBRATION MENU................................................................. 23
6.3.1 Sensor Code ...................................................................... 24
6.3.2 Air Calibration ................................................................... 24
6.3.3 0% Calibration................................................................... 27
6.4 OPTIONS MENU....................................................................... 28
6.4.1 Adjust Time and Date ........................................................ 28
6.4.2 Temperature Offset ........................................................... 29
6.4.3 Device Info and Reset Device.............................................. 29
6.5 ADVANCED MENU.................................................................... 30
7DATA LOGGING.....................................................................31
7.1 START LOGGING ...................................................................... 31
7.1.1 Manual Logging................................................................. 31
7.1.2 Continuous Logging ........................................................... 32
7.2 LOGGING MODE ...................................................................... 33
8STANDBY MODE ................................................................... 35
9PC MODE............................................................................... 35
10 FIRESTINGGO2 MANAGER.....................................................36
10.1 SOFTWARE INSTALLATION......................................................... 36
10.2 OPERATION WITHOUT CONNECTED DEVICE .................................. 36
10.3 OPERATION WITH CONNECTED DEVICE........................................ 36
10.3.1 Live Graph Window ........................................................37
10.3.2 Settings ........................................................................ 40
10.3.3 Calibration .................................................................... 42
10.3.4 Air Calibration ............................................................... 43
10.3.5 0% Calibration .............................................................. 45
10.3.6 Data Logging ................................................................ 47

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10.3.7 Options ......................................................................... 50
10.4 LOG INSPECTOR AND FILE MANAGEMENT .................................... 52
10.4.1 File Management........................................................... 52
10.4.2 Log Inspector Window ................................................... 53
11 WARNINGS ...........................................................................56
11.1 OXYGEN SENSOR WARNINGS..................................................... 56
11.2 TEMPERATURE SENSOR WARNING.............................................. 57
12 CALIBRATION STANDARDS...................................................59
12.1 THE AIR CALIBRATION STANDARD .............................................. 59
12.1.1 Ambient Air................................................................... 61
12.1.2 Water-Vapor Saturated Air ............................................ 61
12.1.3 Air Saturated Water ...................................................... 61
12.2 THE 0% STANDARD.................................................................. 62
12.2.1 Water Mixed with a Strong Reductant............................ 62
12.2.2 Nitrogen Gas................................................................. 63
13 APPENDIX.............................................................................64
13.1 SPECIFICATIONS OF THE FIRESTINGGO2 ......................................64
13.2 MEASURING PRINCIPLE ............................................................. 65
13.3 DEFINITION OF OXYGEN UNITS...................................................66
13.4 EXPLANATION OF THE SENSOR CODE ..........................................68

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1Introduction
TheFireStingGO2 is a hand-held fiber-optic oxygen meter based
on the established FireSting technology featuring:
broad oxygen sensor portfolio (micro- and minisensors, robust
probes, sensor spots, flow-through cells, respiration vials)
sensors for the full and the trace oxygen range
measurements in water as well as in the gas phase
automatic temperature and pressure compensation
proven REDFLASH technology
with New Features:
integrated high contrast LCD display
integrated rechargeable battery and memory
extremely low power consumption for long-term logging
and Operation Modes:
stand-alone via intuitive LCD user interface
with Windows PC via USB

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The new pocket meter FireStingGO2 completes the portfolio of
different fiber-optic oxygen meters from PyroScience, comprising
PC-operated 1-, 2- or 4-channel FireStingO2 meters with a broad
oxygen sensor portfolio and the 1-channel
Piccolo2 meter for advanced applications
of contactless sensors, e.g. in microfluidics.
The FireStingGO2 has integrated memory
and battery and combines the established
FireStingO2 technology with stand-alone
operation and the ability of long-term
logging for >1 year. With this flexibility,
oxygen measurements can be performed
independent of a PC at different locations
using the broad range of fiber-optic
oxygen sensors from PyroScience.
The FireStingGO2impresses by its compactness, functionality and
extremely low power consumption allowing for long-term logging
without the need of recharging. The handling of the FireStingGO2
is intuitive and all information concerning the connected and
internal sensors, measurement and logging parameters are
displayed on a high contrast LCD display.
More information concerning our product portfolio can be found
on our website at www.pyroscience.com or contact us under
[e-mail].
Your PyroScience Team

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2Safety Guidelines
The FireStingGO2is a laboratory instrument to be used with fiber-
optic oxygen sensors (optodes) from PyroScience. In order to
guarantee an optimal performance of the FireStingGO2, please
follow these operation instructions and safety guidelines.
If any problems or damage evolve, please turn the instrument off
and disconnect it immediately. Mark this FireStingGO2to prevent
any further use and consult PyroScience for repair or maintenance
service. The FireStingGO2should not be manipulated or opened by
unauthorized persons, only by PyroScience or persons advised
directly from PyroScience.
Please note that opening the housing will void the warranty. There
are no serviceable parts inside the device.
The FireStingGO2meter and the sensors should be kept and
stored outside the reach of children in a secure place under dry and
clean conditions at room temperature, avoiding moisture, dust,
corrosive conditions and heating of the instrument. This device
and the sensors are not intended for medical, military or other
safety relevant areas. They must not be used for applications in
humans; not for in vivo examination on humans, not for human-
diagnostic or therapeutic purposes. The sensors must not be
brought in direct contact with foods intended for consumption by
humans.
The FireStingGO2has an integrated lithium-ion battery, so please
follow the general safety instructions for their use. The
FireStingGO2is not water-proof and should be used by qualified
personal only, following the operation instructions and safety
guidelines of this manual. Please follow the appropriate laws and
guidelines for safety like EEC directives for protective labor
legislation, national protective labor legislation, safety regulations

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for accident prevention and safety data-sheets from
manufacturers of chemicals used during measurements.
Calibration and application of the sensors, data acquisition, data
processing and data publication is on the user's authority.
When used in the field, the environmental conditions (like high
humidity, dust, exposure to direct solar radiation) may cause
damage or interference of the FireStingGO2, which is on the user's
authority.
Before using the oxygen meter FireStingGO2 and its sensors, read
carefully the available instructions and user manuals.

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In case of problems or damage, turn the instrument off,
disconnect and mark it to prevent any further use! Consult
PyroScience for advice! There are no serviceable parts inside the
device. Opening the housing will void the warranty!
The FireStingGO2 is not water-proof, is sensitive to corrosive
conditions and to changes in temperature causing
condensation. Avoid any condition (e.g. direct sun light) causing
a heating of the device above 50°C (122°F) or below 0°C (32°F).
Avoid elevated humidity causing condensing conditions. Please
follow the safety instructions for integrated lithium-ion
batteries.
Handle the sensors with care especially after removal of the
protective cap! Prevent mechanical stress to the fragile sensing
tip and injuries with needle-type sensors! Avoid strong bending
of the fiber cable!
Calibration and application of the sensors, data acquisition,
treatment and publication is on the user’s authority!
The sensors and the oxygen meter FireStingGO2 are not
intended for medical, diagnostic, therapeutic, or military
purposes or any other safety-critical applications. The sensors
must not be used for applications in humans and must not be
brought in direct contact with foods intended for consumption
by humans.
The FireStingGO2 and sensors should be used in the laboratory
by qualified personnel only, following the user instructions and
the safety guidelines of the manual, as well as the appropriate
laws and guidelines for safety in the laboratory!
Keep the sensors and the oxygen meter FireStingGO2out of
reach of children!

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3Overview FireStingGO2
This chapter provides an overview about the principle components
and general operation procedures of the pocket oxygen meter
FireStingGO2.
3.1 Ports and Integrated Sensors
The FireStingGO2has three ports and one integrated sensor:
micro USB port for charging and communication
oxygen port for connecting a fiber-optic oxygen sensor
temperature port for connecting a temperature sensor
internal air sensor for pressure and relative humidity

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3.1.1 Micro USB Port
The micro USB port is used for recharging the integrated battery of
the FireStingGO2(see below), as well as for data communication
with a PC in combination with the software "FireStingGO2
Manager". This software is especially needed for downloading and
inspecting logged data files from the FireStingGO2device.
3.1.2 Oxygen Port
Fiber-optic oxygen sensors from PyroScience with ST-connectors
are connected to the oxygen port. Please refer to the website of
PyroScience concerning the broad range of available oxygen
sensor types. Remove the protective caps from the oxygen port as
well as from the ST-connector of the oxygen sensor. Then, insert
the ST-connector into the oxygen port and turn the bayonet
coupling gently clockwise until the plug is locked firmly.
3.1.3 Temperature Port
Temperature sensors (4-wire PT100-sensors with LEMO
connectors) are connected to the temperature port by simply
pushing the connector into the port. Please refer to the website of
PyroScience concerning available temperature sensors.
The temperature sensor can be used for automatic temperature
compensation of the oxygen measurement.
3.1.4 Internal Air Sensor
The internal air sensor measures the pressure and the relative
humidity of the ambient air. It is located behind the three little air
inlets located next to the right side of the display. These sensors
are needed for a precise determination of the actual oxygen level,
if the oxygen sensor is calibrated in ambient air. The pressure
sensor is additionally used for automatic pressure compensation of
the oxygen measurement.

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Keep the three little air inlets of the air sensor clean and dry in
order to assure a proper operation of this sensor. Do not cover
these air holes during the measurements.
3.1.5 Power Button and Recharging the Battery
The FireStingGO2is switched on and off by pressing the right
button for ca. 1 second (OK button). Note, that the device cannot
be switched off, while the device is logging data or while it is in the
PC mode (see below).
If the device does not switch on by pressing the OK button, the
integrated battery needs to be recharged. For this, connect the
USB port of the FireStingGO2to any standard micro USB charger
or to any available computer USB port (with the included USB
cable). Typically the battery is fully recharged within ca. 2 hours.
3.2 Stand Clamp
The FireStingGO2 can be optionally mounted on common lab
stands with the included stand clamp. For this, remove the red
silicone shell from the device and mount the stand clamp as shown
in the image.

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3.3 User Interface
The user interface of the FireStingGO2consists of the display and
four buttons. The high-contrast display features ultra-low power
consumption. Therefore it is permanently activated without
significantly reducing the run-time of the rechargeable battery.
The four buttons allow for an intuitive stand-alone operation of the
FireStingGO2.
OK Button: If pressed for 1 second, the device is switched on or
off. If pressed shortly, then this button is generally used for
selecting an item or for confirming a setting. Alternatively, the
lower right corner of the display might show a context specific
function, e.g. MENU, START, NEXT, or SAMPLE.
Back Button: This button is generally used for "going back" e.g. in
menus, or for cancelling an operation. Alternatively, the lower left
corner of the display might show a context specific function, e.g.
CANCEL, BACK, or STOP. If pressed for 10 seconds, a hardware
reset of the device can be performed.
Up and Down Buttons: The context specific functionality of these
buttons is always indicated by small black triangles ( or ) in the
display. They are generally used for selecting items from menus or
dialog screens, but also for adjusting numerical values e.g. when
adjusting the sensor code or a temperature value in the settings.
Depending on the operation mode, there might be several „sub-
screens” available, which is indicated by small black triangles ( or
) appearing in the upper or lower line of the display. By pressing
the up or down buttons, the screen content will scroll up or down,
revealing the other sub-screens.

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3.4 Operation Modes
The user interface of the FireStingGO2meter comprises five
different operation modes: Live Mode, Menu Mode, Logging
Mode, Standby Mode, and PC Mode.
3.4.1 Live Mode
After switching on the FireStingGO2, the device
starts in the Live Mode. Here the device performs a
measurement every 2 seconds and shows the
results in altogether four sub-screens. The data are
not logged in this mode. For more details on the
Live mode refer to chapter 5.
3.4.2 Menu Mode
The Menu Mode can be opened in the Live Mode
by pressing the OK button. The menu is used for:
adjusting the settings
performing sensor calibration
starting data logging
accessing diverse options
After exiting the menu mode with the BACK button, the device
reverts to the Live Mode. For details on the menu mode refer to
chapter 6.
3.4.3 Logging Mode
The Logging Mode is started by selecting "Start
Logging" in the main menu and pressing the OK
button. In the logging mode the measured data
points (sensor readings) are saved in a log file
within the internal data memory of the device.
Altogether six sub-screens provide the user with
detailed information about the progress of the
data logging. If the Logging Mode is terminated, the device will

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revert to the Live Mode. For more details on the logging mode
refer to chapter 7.
3.4.4 Standby Mode
The device automatically enters the Standby
Mode, if the user did not press any button within 5
minutes in the Live Mode. Here the measurements
are stopped to prolong the lifetime of the
connected oxygen sensor. For details on the
Standby Mode refer to chapter 8.
3.4.5 PC Mode
If the device is connected to a PC with the included
USB cable and the software FireStingGO2
Manager is started, the device enters the PC
Mode. This is indicated by the "PC" symbol in the
upper right corner of the display. Here the Menu
Mode is not accessible, only the sub-screens are
accessible with the UP and DOWN buttons. For more details on the
PC mode refer to chapter 9.
3.5 Status Line
In all modes except for the Menu Mode, the status line is shown in
the upper line of the display.
The battery symbol indicates the actual charge level of the internal
battery. The plug symbol indicates that an external power supply is
connected to the USB port. An arrow symbol between the plug and
the battery symbol indicates that charging is in progress. And the
PC symbol indicates that the device is in the PC Mode after the

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FireStingGO2Manager software has been started on an external
computer (connected via the USB port).
In the Live, PC or Logging Mode, an additional bar graph and a
numerical value show the so-called "signal intensity". It provides
important information on the actual condition and remaining
lifetime of the connected oxygen sensor.
Potential warnings (Low Signal, No Signal, Too High, Bad Ref)
regarding problems with the oxygen sensor signal are displayed at
the position of the bar graph. For more details about trouble
shooting these warnings refer to chapter 11.
Do not continue with measurements if a warning is shown!

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4Quick Start
4.1 Getting Started
Connect the oxygen sensor and optionally the temperature sensor
to the corresponding ports of the FireStingGO2(chapter 3.1). Start
the FireStingGO2 meter by pressing the OK button for about 1 sec.
Check the battery charge level of the device in the status line
(chapter 3.5) and recharge the battery if necessary (chapter 3.1.5).
Select MENU with the OK button and first Adjust the local Time
and Date in the Options Menu (chapter 6.4.1)
4.2 Settings and Options Adjustments
For details refer to chapter 6.2.
Open the Settings Menu, enter the Sensor Code of the connected
oxygen sensor, select the sample Medium and choose your
preferred Oxygen Units. Adjust the sample Temperature used for
the temperature compensation of the oxygen measurements
(Sensor or Fixed). If applicable, adjust the sample Salinity.
4.3 Sensor Calibration
For details refer to chapter 6.3.
Open the Calibration Menu in the main menu and review if the
correct Sensor Code was adjusted. Prepare appropriate air and 0%
calibration standards. Open Calibrate Air and select the type of
temperature compensation used during the calibration: Sensor for
automatic temperature compensation with the connected
temperature sensor or Fixed with a defined and constant
calibration temperature (needs to be measured and controlled).
Then select the appropriate calibration medium equivalent to the
sample under investigation: Ambient Air for gas measurements
and Air Saturated Water for measurements in aqueous samples.
Insert the oxygen and temperature sensor into the air calibration
standard, wait for steady state and press SET AIR.

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After a successful air calibration, open Calibrate 0%, select the
type of temperature compensation used during the calibration
(Sensor or Fixed, see above), insert the oxygen and temperature
sensor into the 0% calibration standard, wait for steady state and
press SET 0%.
4.4 Live Measurements
Exit the menu by pressing the BACK button. Measurements are
performed now in the Live Mode every 2 seconds. Access the
different sub-screens with the UP and DOWN buttons, showing
the measurement results. Note, that the data are NOT logged in
the Live Mode.
4.5 Data logging
For details refer to chapter 7.
Open Start Logging in the Main Menu. Choose the appropriate
mode: Manual for manual logging, i.e. data points are logged each
time the OK button (SAMPLE) is pressed; Continuous for
automatized logging with adjustable Logging Interval and
Logging Duration. The logged data are displayed numerically and
graphically on several sub-screens. If the data logging is finished,
EXIT the Logging Mode with the BACK button.
4.6 Data management and inspection
For details refer to chapter 10.
Download and install the FireStingGO2Manager software from
the PyroScience website to a Windows PC. Connect the
FireStingGO2device with the included USB cable, and start the
software. Go to the File tab, select a log file and click on Download
& Inspect. This will download the log file from the device to the
PC, and show the logged data in the Log Inspector window.

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5Live Mode
After power-up or after activation
from standby the FireStingGO2is in
the Live Mode. Measurements are
performed every 2 seconds. The
different sub-screens can be
accessed with the UP and DOWN
buttons. Note, that the data are
NOT logged in the Live Mode.
5.1 Top Screen
Here, the current reading of the
connected oxygen sensor and the
selected oxygen unit (see chapter
6.2.3) is shown, as well as the
compensation temperature (sensor
or fixed, see chapter 6.2.4).
5.2 Sub-screen 1
Here the sensor code and the dates
of the last Calibrations are shown
(see chapter 6.3).
5.3 Sub-screen 2
This sub-screen shows details on the Environment, including the
readings of the temperature sensor (if connected) and of the
internal air sensor, including the air pressure (mbar) and relative
humidity (%RH) (see chapter 3.1.4).
5.4 Sub-screen 3
This sub-screen shows the Clock with the actual time and date.
Refer to chapter 6.4.1 how to set the clock.