pyroscience Pico-O2 User manual

PyroScience GmbH www.pyroscience.com
Pico-O2
OEM Fiber-Optic Oxygen Meter
MANUAL
O2

Pico-O2 | Manual
© PyroScience GmbH 2
Pico-O2
OEM Fiber-Optic Oxygen Meter
Document Version 1.08
The Pico-O2 is released by:
PyroScience GmbH
Kackertstr. 11
52072 Aachen
Germany
Phone [phone]
Fax [phone]
Email [e-mail]
Web www.pyroscience.com
Registered: Aachen HRB 17329, Germany

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TABLE OF CONTENT
1Introduction .....................................................................................................................................5
2Overview..........................................................................................................................................6
2.1 Oxygen sensors................................................................................................................................6
2.1.1 Robust probes .....................................................................................................................6
2.1.2 Contactless sensor spots.................................................................................................7
2.1.3 Flow-through cells.............................................................................................................7
2.1.4 Vials.........................................................................................................................................8
2.1.5 Oxygen sensitive nanoparticles...................................................................................8
2.2 Connection of probes, fibers and rods to the optical port.............................................8
2.3 External temperature sensor......................................................................................................9
2.4 Status LED .......................................................................................................................................10
2.5 USB interface cable .....................................................................................................................10
3Option 1: Operating the Module with Pyro Workbench.................................................... 11
3.1 Installing the software Pyro Workbench............................................................................11
3.2 Using the software Pyro Workbench ...................................................................................12
4Option 2: Operating the module with Pyro Developer Tool ............................................ 13
4.1 Installing the software Pyro Developer Tool....................................................................13
4.2 Using the software Pyro Developer Tool ...........................................................................14
5Option 3: Simplified Custom Integration............................................................................... 15
5.1 Configuring the Module using PyroScience Software ...................................................15
5.2 Electrical Connector for Custom Integration.....................................................................15
5.3 Configuration of the Serial Interface....................................................................................16
5.4 Communication Protocol ...........................................................................................................18
5.4.1 General Definitions.........................................................................................................18
5.4.2 MEA –Trigger Measurement ......................................................................................18
5.4.3 CHI –Calibrate oxygen Sensor at ambient air ....................................................21
5.4.4 CLO –Calibrate oxygen Sensor at 0% (anoxic)....................................................21
5.4.5 SVS –Save Configuration Permanently in Flash Memory..............................21
5.4.6 #VERS –Get Device Information ..............................................................................22
5.4.7 #IDNR –Get Unique ID Number................................................................................23

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5.4.8 #LOGO –Flash Status LED...........................................................................................23
5.4.9 #PDWN –Power Down Sensor Circuits .................................................................23
5.4.10#PWUP –Power Up Sensor Circuits........................................................................24
5.4.11#STOP –Enter Deep Sleep Mode..............................................................................24
5.4.12#RSET –Reset Device....................................................................................................24
5.4.13#RDUM –Read User Memory ....................................................................................24
5.4.14#WRUM –Write User Memory ..................................................................................25
5.4.15#ERRO –Response if Error Occurred......................................................................25
5.5 Available Implementations of Communication Protocol..............................................28
6Option 4: Advanced Custom Integration ............................................................................... 29
7Technical Drawing....................................................................................................................... 30
8Specifications ............................................................................................................................... 31
9Safety Guidelines......................................................................................................................... 33

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1INTRODUCTION
The Pico-O2 (item no. PICO-O2) is a fiber-optic OEM meter for read-out of optical oxygen
sensors from PyroScience. The Pico-O2 measures oxygen gas (e.g. %O2) or dissolved
oxygen in liquids (DO) and the firmware provides internal calculation of all major
oxygen units taking into account adjustable values of temperature, pressure, humidity,
and salinity. This OEM module is easy to integrate and is controlled with a simple serial
communication protocol. To control the Pico-O2,there are several options depending on
the users´ level of experience with optical sensors:
Option 1: For initial evaluation purposes, Pico-O2 can be operated with the
simple and customer-friendly logger software Pyro Workbench, which is
typically used by end-users. This software offers comfortable settings and
calibration wizards, as well as advanced logging features. Several modules can
be operated in parallel within a single window. This software requires an
encoded USB interface cable (item no. PICO-USB) for connecting the module to a
Windows PC (see chapter 3).
Option 2: For advanced evaluation purposes, the module can be operated with
the software Pyro Developer Tool. It offers simple settings and calibration
procedures, as well as basic logging features. Furthermore, additional advanced
settings offer full control on all features of the module. This software requires
an encoded USB-interface cable (item no. PICO-USB) for connecting the module
to a Windows PC (see chapter 4).
Option 3: A simplified custom integration of the module can be realized by
adjusting the settings and performing sensor calibrations using the PyroScience
software Pyro Workbench or Pyro Developer Tool (requires the encoded USB
interface cable PICO-USB). After closing the software, the configuration is
automatically saved within the internal flash memory of the module. The
module can then be integrated into a specific setup, and your custom software
can perform measurements using a proprietary USB/UART communication
protocol (see chapter 5).
Option 4: For advanced custom integration the full USB/UART communication
protocol is available on request, allowing custom software full control on all
settings, calibration and measurement features of the module (see chapter 6).

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2OVERVIEW
Figure 1 provides an overview of the Pico-O2.The front provides the port for
connecting an optical fiber used for read-out of optical oxygen sensors, as well as solder
points for an external temperature sensor enabling automatic temperature
compensation. The backside of the module provides the connector for the power supply
and the digital communication interface, as well as a red status LED.
Figure 1: Overview of Pico-O2
2.1 Oxygen sensors
The Pico-O2 is compatible to a variety of oxygen sensors from PyroScience such as
directly connected robust probes and dipping probes, but also contactless sensors
including sensor spots, sensor vials, flow-through cells and nanoprobes which are read-
out utilizing an optical fiber. Different applications and sensor formats require different
type of optical fiber.
2.1.1 Robust probes
The robust oxygen probe OPROB3 is a fiber based optical sensor. The fiber has a length
of 1 meter and is connected to the optical port of the Pico-O2. The dipping probe
OPDIP20 is made out of stainless steel and has a length of 20 mm. Both probes have a
diameter of 3 mm and are applicable in gas and liquid phase.

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Figure 2: Setup of robust oxygen probe OPROB3 with Pico-O2 (up) and OPDIP20 with Pico-O2 (down).
2.1.2 Contactless sensor spots
There are different sensor spots for the oxygen measurement in gas and liquid phase
available. The standard sensor spots for oxygen measurements are the OXSP5. Sensor
spots are mounted inside of transparent vessels. For mounting of oxygen sensor spots,
please refer to the corresponding “Manual oxygen sensors”. Sensor spots are connected
to the Pico-O2 via an optical fiber. To place the fiber behind the sensor spots on the
outside of the transparent vessel, an adapter (SPADBAS) is necessary. The optical fiber
PICFIB2 can be used for a wall thickness of 0-10 mm. Moreover, rods can be used
instead of fibers when the Pico-O2 is placed directly behind the sensor spot. Depending
on the wall thickness of the transparent container, two different rods can be used.
PICROD2 can be used for oxygen read-out through a wall thickness of 0-10 mm and
PICROD3 for 10-20 mm.
Figure 3: Connection of oxygen sensor spots to Pico-O2 via an optical fiber (left) or a rod (right).
2.1.3 Flow-through cells
Oxygen flow-through cells (e.g. OXFLOW) can be connected via the optical fiber PICFIB2
to a Pico-O2. In contrast to the sensor spots, no additional adapter is needed.

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Figure 4: Connection of oxygen flow-through cell to Pico-O2 via an optical fiber.
2.1.4 Vials
Oxygen vials (e.g. OXVIAL20) can be connected via the optical fiber PICFIB2 to a PICO-
O2. To connect the optical fiber to the vial, an adapter (ADVIAL20T) is necessary.
Figure 5: Connection of an oxygen vial to Pico-O2 via an optical fiber.
2.1.5 Oxygen sensitive nanoparticles
Oxygen sensitive nanoparticles (OXNANO) are submersed in liquid. They were mostly
applied in microwell plates or in microfluidic applications. For read-out, rods (PICROD2)
or fibers (PICFIB2) are used. The rod and fiber can be fixated via an adapter (SPADBAS)
to the microwell plate. For microfluidic applications a rod or a fiber with an additional
lens should be used (PICROD2-LNS and PICFIB2-LNS). The fixation has to be customized.
2.2 Connection of probes, fibers and rods to the optical
port
The robust probe, the dipping probe, the optical fibers or the fiber rods must be
connected to the optical port of the Pico-O2 with their instrument end (IE). At the IE, the
core of the optical fiber protrudes ca. 1 mm from the fiber jacket. The instrument end IE
is marked with "→Instrument" on the attached label. For PICROD2, the AE is indicated
via a black ring.

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First, remove the protective caps from both ends of the optical probe / fiber / rod. Then
slightly unscrew and loosen the nut at the sensor port of the Pico-O2. The nut must not
be removed from the sensor port. Typically, unscrewing the nut with a single half turn
(180°) is sufficient. Insert the instrument end IE carefully as deep as possible into the
optical port of the Pico-O2. The insertion length is about 24 mm. Fix the optical probe /
fiber / rod by screwing down the nut tightly onto the sensor port of the Pico-O2.
When using contactless sensors, insert the adapter end (AE) of the optical fibers/rods
into the basic spot adapter, adapter ring or flow-through cell and fix it with the clamping
screw.
For robust and dipping probe, insert the sensor end (SE) into the gas or stirred aqueous
liquid sample.
2.3 External temperature sensor
The signal of oxygen sensors is temperature dependent, which can be automatically
compensated. Pico-O2 offers a high-precision sensor interface, which can be directly
connected to a Pt100 temperature sensor (not included, item no. TSUB21-NC). The
temperature sensor has to be soldered to the 4 solder pads at the front of the module.
Then temperature sensor has to be placed into the sample of the oxygen measurement.
Figure 6: Connecting a resistive temperature sensor to the module
The Pt100 temperature sensor has to be soldered to the 4 solder pads at the front of the
module (Figure 6). For short distances (e.g. 10 cm) a simple 2-wire connection might be
sufficient. For this, it is important to shortcut the outer with the inner solder pads as
indicated in Figure 6. For longer distances and/or for high precision measurements a 4-
wire connection should be preferred.

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In order to minimize potential electrical noise coupling into the external
temperature sensor, the cables should be twisted and kept as short as possible.
2.4 Status LED
The behavior of the status LED is given in Table 1.
Table 1: Status LED
2.5 USB interface cable
For the operation of Pico-O2 with a Windows PC, a coded USB interface cable (item no.
PICO-USB) is available from PyroScience. It includes a license for the comfortable logger
software Pyro Workbench and the software Pyro Developer Tool. Especially for initial
testing purposes this software packages can speed up OEM-developments significantly.
Additionally, the USB interface cable PICO-USB provides a virtual COM-port. Custom
software can use this virtual COM-port for communicating directly with the module
based on the communication protocol.
Status
Description
Behavior of status LED
Power-Up
The power supply is switched
on.
A correct startup of the module
is indicated by 4 flashes within
1-2 seconds.
Active
The module is either in idle
mode waiting for a new
command, or it is executing a
command.
The LED flashes periodically
with 1s interval.
Deep sleep
While the power supply is still
enabled, the module can be put
into deep sleep mode by the
#STOP command.
The LED is switched off.
#LOGO-command
The #LOGO-command is sent to
the module.
The LED flashes 4 times within
1-2 seconds.