pyroscience FireStingO2 User manual

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FireStingO2
FIBER-OPTIC OXYGEN &TEMPERATURE METER
USER MANUAL

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Document Version 3.081
Refers to Pyro Oxygen Logger Software version >3.3
The FireStingO2 is manufactured by
PyroScience GmbH
Hubertusstr. 35
52064 Aachen
Germany
Phone [phone]
Fax [phone]
Email [e-mail]
Internet www.pyroscience.com
Registered: Aachen HRB 17329, Germany

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TABLE OF CONTENT
1OVERVIEW ..............................................................................5
2SAFETY GUIDELINES...............................................................7
3INTRODUCTION TO THE FIRESTING METER...........................10
4SOFTWARE INSTALLATION...................................................12
5OPTICAL OXYGEN & TEMPERATURE SENSORS .....................13
5.1 OVERVIEW OF SENSOR TYPES .................................................... 13
5.2 CONNECTING THE SENSORS....................................................... 14
5.3CLEANING AND MAINTENANCE OF THE SENSORS........................... 15
6THE SOFTWARE "PYRO OXYGEN LOGGER" ........................... 17
6.1 FIRESTING SETTINGS ................................................................ 18
6.1.1 Channel Tab: Optical Oxygen Sensors ................................ 19
6.1.2 Channel Tab: Optical Temperature Sensors ........................ 24
6.1.3 Temperature Tab ............................................................... 24
6.1.4 Options Tab....................................................................... 26
6.2 MAIN WINDOW........................................................................ 28
6.2.1 Chart Recorder .................................................................. 29
6.2.2 Warnings........................................................................... 32
6.2.3 Measurement and Logging................................................. 33
6.2.4 Raw Data Window..............................................................37
6.3 OVERVIEW PANEL ....................................................................40
6.4 DATA FILE PANEL..................................................................... 41
7SENSOR CALIBRATION .........................................................42
7.1 OPTICAL OXYGEN SENSOR CALIBRATION..................................... 43
7.1.1 Calibration Mode: Factory .................................................. 45
7.1.2 Calibration Mode: 1-Point in Ambient Air ............................ 46
7.1.3 Calibration Mode: 1-Point in Water or Humid Air ................. 49
7.1.4 Calibration Mode: 2-Point in Ambient Air............................ 51
7.1.5 Calibration Mode: 2-Point in Water or Humid Air................. 55
7.1.6 Calibration Mode: Custom Mode ........................................ 59
7.2 OPTICAL TEMPERATURE SENSOR CALIBRATION ............................ 65

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8OXYGEN CALIBRATION STANDARDS ....................................68
8.1 THE AIR CALIBRATION STANDARD ..............................................68
8.1.1 Ambient Air ....................................................................... 70
8.1.2 Water-Vapor Saturated Air .................................................71
8.1.3 Air Saturated Water............................................................71
8.2 THE 0% OXYGEN STANDARD..................................................... 72
8.2.1 Water Mixed with a Strong Reductant ................................ 72
8.2.2 Water Flushed with Nitrogen Gas........................................73
8.2.3 Nitrogen Gas ......................................................................73
9CALIBRATION OF CONTACTLESS SENSORS ..........................74
9.1 CALIBRATION PROCEDURE......................................................... 74
9.2 MANUAL BACKGROUND COMPENSATION .................................... 75
10 TEMPERATURE MEASUREMENT............................................ 77
10.1 EXTERNAL (PT100) TEMPERATURE SENSORS................................77
10.2 INTERNAL TEMPERATURE SENSOR ...............................................77
10.3 OPTICAL TEMPERATURE SENSORS .............................................. 78
10.4 AUTOMATIC TEMPERATURE COMPENSATION................................ 78
11 ANALOG OUTPUT AND AUTO-MODE ....................................81
11.1 ANALOG OUTPUT .................................................................... 81
11.2 AUTO-MODE...........................................................................84
11.3 ADVANCED AUTO-MODE ..........................................................86
12 APPENDIX............................................................................ 88
12.1 SPECIFICATIONS OF THE FIRESTINGO2.........................................88
12.2 EXTENSION PORT X1 ................................................................90
12.2.1 Connector X1 (Power, Digital Interface, Analog In).......... 90
12.2.2 Connector X2 (Analog Output) ....................................... 95
12.3 TROUBLESHOOTING .................................................................96
12.4 OXYGEN MEASURING PRINCIPLE ................................................ 97
12.5 OPERATING SEVERAL FIRESTINGO2 IN PARALLEL...........................99
12.6 DEFINITION OF OXYGEN UNITS................................................. 100
12.7 TABLE OF OXYGEN SOLUBILITY ................................................ 102
12.8 EXPLANATION OF THE SENSOR CODE ........................................ 104

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1Overview
The compact USB-powered fiber-optic meter FireStingO2 with 1,
2, or 4 channels unifies several innovative technological
improvements making it the new standard of oxygen sensing with
fiber-optical oxygen sensors (optodes). The FireStingO2 utilizes a
measuring principle based on red light excitation and lifetime
detection in the near infrared using luminescent oxygen indicators
(REDFLASH technology, see Appendix 12.4). It is a multifunctional
oxygen meter working
•for oxygen measurements in water as well as in gas phases,
•with fiber-based sensors comprising several size classes
like microsensors, minisensors, or robust probes,
•with contactless sensors for oxygen and temperature like
sensor spots, flow-through cells or respiration vials, and
•with full range and trace oxygen sensors,
•with optical temperature minisensors.
The FireStingO2 has integrated atmospheric pressure and
humidity sensors for a precise and easy sensor calibration, but also
for automatic pressure compensation of the oxygen
measurements. Furthermore, the FireStingO2 offers 4 analog
outputs and a built-in temperature port for an external PT100
temperature probe for automatic temperature compensation of
the oxygen measurement. If independent temperature
compensation for all oxygen channels is needed, a USB-powered
temperature extension module TeX4 can be easily fixed below the
FireStingO2 meter with a smart docking mechanism. Or the new
optical temperature sensors can be connected to any channel of
the FireStingO2 meter. By placing the same sensor type for
oxygen and temperature into the sample, true temperature
compensation of the oxygen measurements can be achieved.

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The user-friendly Pyro Oxygen Logger software allows operation
of several FireStingO2 meters in parallel as a scalable multi-
channel system.
Along with the FireStingO2 we offer turnkey motorized setups for
measurements of depth-profiles in semi-solid environmental
samples and along micro-gradients at high temporal and spatial
resolution. And several customized OEM solutions are available for
application in industry and underwater.
More information concerning our products can be found at
www.pyroscience.com
or contact us at [e-mail].
Your PyroScience Team

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2Safety Guidelines
The FireStingO2 is a laboratory instrument to be used with fiber-
optic oxygen and temperature sensors (optodes) from
PyroScience. In order to guarantee an optimal performance of the
FireStingO2, please follow these operation instructions and safety
guidelines.
If any problems or damage evolve, disconnect the instrument
immediately, mark it to prevent any further use and consult
PyroScience for repair or maintenance service. The FireStingO2
should not be manipulated or opened by unauthorized persons,
only by PyroScience or persons advised directly from PyroScience.
Please note that opening the housing will void the warranty. There
are no serviceable parts inside the device.
The FireStingO2 and sensors should be kept and stored outside the
reach of children in a secure place under dry and clean conditions
at room temperature, avoiding moisture, dust, corrosive
conditions and heating of the instrument. This device and the
sensors are not intended for medical, military or other safety-
relevant areas. They must not be used for applications in humans;
not for in vivo examination on humans, not for human-diagnostic
or therapeutic purposes. The sensors must not be brought in direct
contact with foods intended for consumption by humans.
The FireStingO2 should be used in the laboratory by qualified
personal only, following the operation instructions and safety
guidelines of this manual. Please follow the appropriate laws and
guidelines for safety, like EEC directives for protective labor
legislation, national protective labor legislation, safety regulations
for accident prevention and safety data-sheets from
manufacturers of chemicals used during the measurements.

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Calibration and application of the sensors, data acquisition, data
processing and data publication is on the user's authority.
When used in the field, the environmental conditions (like high
humidity, dust, exposure to direct solar radiation) may cause
damage or interference of the FireStingO2, which is on the user's
authority.

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Before using the FireStingO2 and its sensors, read carefully the
instructions and user manual for the oxygen meter FireStingO2.
In case of problems or damage, disconnect the instrument and
mark it to prevent any further use! Consult PyroScience for
advice! There are no serviceable parts inside the device. Please
note that opening the housing will void the warranty!
The FireStingO2 is not watertight, is sensitive to corrosive
conditions and to changes in temperature causing
condensation. Avoid any condition (e.g. direct sun light) causing
a heating of the device above 50°C (122°F) or below 0°C (32°F).
Avoid any elevated humidity causing condensing conditions.
Handle the sensors with care especially after removal of the
protective cap! Prevent mechanical stress to the fragile sensing
tip! Avoid strong bending of the fiber cable! Prevent injuries
with needle-type sensors!
Calibration and application of the sensors is on the user’s
authority, as well as data acquisition, treatment and
publication!
The sensors and the oxygen meter FireStingO2 are not intended
for medical, diagnostic, therapeutic, or military purposes or any
other safety-critical applications. The sensors must not be used
for applications in humans and must not be brought in direct
contact with foods intended for consumption by humans.
The FireStingO2 and sensors should be used in the laboratory by
qualified personnel only, following the user instructions and the
safety guidelines of the manual, as well as the appropriate laws
and guidelines for safety in the laboratory!
Keep the sensors and the oxygen meter FireStingO2 out of
reach of children!

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3Introduction to the FireSting Meter
The FireStingO2 is an optical oxygen meter that is compatible with
a broad range of optical oxygen and temperature sensors from
PyroScience:
microsensors and minisensors (retractable needle-type,
fixed needle-type, or bare fiber),
robust probes and
contactless sensors (sensor spots, respiration vials, flow-
through cells)
Most oxygen sensors are available in versions for the full range (0-
50% O2, max. range 0-100% O2) and for the trace range (0-10%
O2). The optical detection technology is based on the oxygen-
sensitive REDFLASH indicators which use red light excitation and
lifetime detection in the near infrared (see Appendix 12.4 for more
details).
The FireStingO2 is a compact USB-powered fiber-optic oxygen
meter with 1, 2 or 4 channels for oxygen and temperature
measurements in the laboratory. The optical temperature sensors
can be used for true automatic temperature compensation of the
oxygen measurements. Additionally, one external PT100
temperature probe can be connected for calibration of the optical
temperature sensors and for automatic temperature
compensation of the oxygen measurements. The integrated
sensors for atmospheric pressure (mbar) and relative humidity (%
RH) of the ambient air enable a precise and easy sensor calibration,
as well as automatic pressure compensation of the oxygen
measurements.
The FireStingO2 is operated via a Micro-USB connection to a PC /
tablet with a Windows operation system. The included logging
software Pyro Oxygen Logger provides comfortable calibration
and logging functionality.