Qiagen RT2 First Strand Kit User manual

RT² Profiler PCR Array (Rotor-Gene®Format)
Human Obesity
Cat. no. 330231 PAHS-017ZR
For pathway expression analysis
Format For use with the following real-time cyclers
RT² Profiler PCR Array,
Format R
Rotor-Gene Q, other Rotor-Gene cyclers
Description
The Human Obesity RT² Profiler PCR Array profiles the expression of 84 genes related to
obesity. The control of a relative stable body weight depends on a complex interaction
among the hormone axes in the peripheral nervous system and neurotransmitter signals in
the central nervous system that play a crucial role in maintaining a balanced energy
metabolism. This PCR Array includes obesity-related genes that are directly involved in the
regulation of energy intake and expenditure. The genes encode for orexigenic peptides,
hormones, and receptors; anorectic peptides, hormones, receptors; and central and
peripheral signaling molecules involved in energy expenditure. Using real-time PCR, you
can easily and reliably analyze expression of a focused panel of genes related to Obesity
with this array.
For further details, consult the RT² Profiler PCR Array Handbook.
Shipping and storage
RT² Profiler PCR Arrays in the Rotor-Gene format are shipped at ambient temperature, on
dry ice, or blue ice packs depending on destination and accompanying products.
For long term storage, keep plates at –20°C.
Note: Ensure that you have the correct RT² Profiler PCR Array format for your real-time
cycler (see table above).
Note: Open the package and store the products appropriately immediately on receipt.
Sample & Assay Technologies

Array layout
The 96 real-time assays in the Rotor-Gene format are located in wells 1–96 of the Rotor-Disc™ (plate
A1–A12=Rotor-Disc 1–12, plate B1–B12=Rotor-Disc 13–24, etc.). To maintain data analysis
compatibility, wells 97–100 do not contain real-time assays but will contain master mix to account
for weight balance.
Gene table: RT² Profiler PCR Array
Position UniGene GenBank Symbol Description
A01 Hs.531719 NM_001117 ADCYAP1 Adenylate cyclase activating polypeptide 1 (pituitary)
A02 Hs.377783 NM_001118 ADCYAP1R1 Adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
A03 Hs.80485 NM_004797 ADIPOQ Adiponectin, C1Q and collagen domain containing
A04 Hs.5298 NM_015999 ADIPOR1 Adiponectin receptor 1
A05 Hs.371642 NM_024551 ADIPOR2 Adiponectin receptor 2
A06 Hs.247686 NM_000682 ADRA2B Adrenergic, alpha-2B-, receptor
A07 Hs.99913 NM_000684 ADRB1 Adrenergic, beta-1-, receptor
A08 Hs.104633 NM_001138 AGRP Agouti related protein homolog (mouse)
A09 Hs.591940 NM_000482 APOA4 Apolipoprotein A-IV
A10 Hs.276252 NM_139321 ATRN Attractin
A11 Hs.502182 NM_001709 BDNF Brain-derived neurotrophic factor
A12 Hs.121484 NM_001727 BRS3 Bombesin-like receptor 3
B01 Hs.529053 NM_000064 C3 Complement component 3
B02 Hs.37058 NM_001741 CALCA Calcitonin-related polypeptide alpha
B03 Hs.489127 NM_001742 CALCR CALCITONIN RECEPTOR
B04 Hs.1707 NM_004291 CARTPT CART prepropeptide
B05 Hs.458426 NM_000729 CCK Cholecystokinin
B06 Hs.129 NM_000730 CCKAR Cholecystokinin A receptor
B07 Hs.1340 NM_001832 CLPS Colipase, pancreatic
B08 Hs.75110 NM_016083 CNR1 Cannabinoid receptor 1 (brain)
B09 Hs.129966 NM_001842 CNTFR Ciliary neurotrophic factor receptor
B10 Hs.446079 NM_001304 CPD Carboxypeptidase D
B11 Hs.707992 NM_001873 CPE Carboxypeptidase E
B12 Hs.417628 NM_004382 CRHR1 Corticotropin releasing hormone receptor 1
C01 Hs.2624 NM_000794 DRD1 Dopamine receptor D1
C02 Hs.73893 NM_000795 DRD2 Dopamine receptor D2
C03 Hs.278959 NM_015973 GAL Galanin prepropeptide
C04 Hs.272191 NM_001480 GALR1 Galanin receptor 1
C05 Hs.516494 NM_002054 GCG Glucagon
C06 Hs.208 NM_000160 GCGR Glucagon receptor
C07 Hs.567275 NM_000515 GH1 Growth hormone 1
C08 Hs.406754 NM_022557 GH2 Growth hormone 2
C09 Hs.125180 NM_000163 GHR Growth hormone receptor
C10 Hs.590080 NM_016362 GHRL Ghrelin/obestatin prepropeptide
C11 Hs.248115 NM_004122 GHSR Growth hormone secretagogue receptor
C12 Hs.389103 NM_002062 GLP1R Glucagon-like peptide 1 receptor
D01 Hs.153444 NM_002091 GRP Gastrin-releasing peptide
D02 Hs.567282 NM_005314 GRPR Gastrin-releasing peptide receptor
D03 Hs.158348 NM_001524 HCRT Hypocretin (orexin) neuropeptide precursor
D04 Hs.388226 NM_001525 HCRTR1 Hypocretin (orexin) receptor 1
D05 Hs.1570 NM_000861 HRH1 Histamine receptor H1
D06 Hs.149037 NM_000868 HTR2C 5-hydroxytryptamine (serotonin) receptor 2C
D07 Hs.46835 NM_000415 IAPP Islet amyloid polypeptide
D08 Hs.1722 NM_000575 IL1A Interleukin 1, alpha
D09 Hs.126256 NM_000576 IL1B Interleukin 1, beta
D10 Hs.701982 NM_000877 IL1R1 Interleukin 1 receptor, type I
D11 Hs.654458 NM_000600 IL6 Interleukin 6 (interferon, beta 2)
D12 Hs.709210 NM_000565 IL6R Interleukin 6 receptor
E01 Hs.654579 NM_000207 INS Insulin
E02 Hs.465744 NM_000208 INSR Insulin receptor
E03 Hs.194236 NM_000230 LEP Leptin
E04 Hs.705413 NM_002303 LEPR Leptin receptor
E05 Hs.248018 NM_019888 MC3R Melanocortin 3 receptor
E06 Hs.248122 NM_005297 MCHR1 Melanin-concentrating hormone receptor 1
E07 Hs.386470 NM_021077 NMB Neuromedin B
E08 Hs.654478 NM_002511 NMBR Neuromedin B receptor
E09 Hs.418367 NM_006681 NMU Neuromedin U

Position UniGene GenBank Symbol Description
E10 Hs.471619 NM_006056 NMUR1 Neuromedin U receptor 1
E11 Hs.1832 NM_000905 NPY Neuropeptide Y
E12 Hs.519057 NM_000909 NPY1R Neuropeptide Y receptor Y1
F01 Hs.122926 NM_000176 NR3C1 Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
F02 Hs.494312 NM_006180 NTRK2 Neurotrophic tyrosine kinase, receptor, type 2
F03 Hs.80962 NM_006183 NTS Neurotensin
F04 Hs.590869 NM_002531 NTSR1 Neurotensin receptor 1 (high affinity)
F05 Hs.106795 NM_000912 OPRK1 Opioid receptor, kappa 1
F06 Hs.2353 NM_000914 OPRM1 Opioid receptor, mu 1
F07 Hs.1897 NM_000939 POMC Proopiomelanocortin
F08 Hs.103110 NM_005036 PPARA Peroxisome proliferator-activated receptor alpha
F09 Hs.162646 NM_015869 PPARG Peroxisome proliferator-activated receptor gamma
F10 Hs.527078 NM_013261 PPARGC1A Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
F11 Hs.248119 NM_004248 PRLHR Prolactin releasing hormone receptor
F12 Hs.417549 NM_002827 PTPN1 Protein tyrosine phosphatase, non-receptor type 1
G01 Hs.169249 NM_004160 PYY Peptide YY
G02 Hs.25691 NM_005856 RAMP3 Receptor (G protein-coupled) activity modifying protein 3
G03 Hs.522087 NM_005866 SIGMAR1 Sigma non-opioid intracellular receptor 1
G04 Hs.485195 NM_002959 SORT1 Sortilin 1
G05 Hs.12409 NM_001048 SST Somatostatin
G06 Hs.514451 NM_001050 SSTR2 Somatostatin receptor 2
G07 Hs.187861 NM_000461 THRB Thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene
homolog 2, avian)
G08 Hs.241570 NM_000594 TNF Tumor necrosis factor
G09 Hs.182231 NM_007117 TRH Thyrotropin-releasing hormone
G10 Hs.534363 NM_003353 UCN Urocortin
G11 Hs.249211 NM_021833 UCP1 Uncoupling protein 1 (mitochondrial, proton carrier)
G12 Hs.524920 NM_053023 ZFP91 Zinc finger protein 91 homolog (mouse)
H01 Hs.520640 NM_001101 ACTB Actin, beta
H02 Hs.534255 NM_004048 B2M Beta-2-microglobulin
H03 Hs.592355 NM_002046 GAPDH Glyceraldehyde-3-phosphate dehydrogenase
H04 Hs.412707 NM_000194 HPRT1 Hypoxanthine phosphoribosyltransferase 1
H05 Hs.546285 NM_001002 RPLP0 Ribosomal protein, large, P0
H06 N/A SA_00105 HGDC Human Genomic DNA Contamination
H07 N/A SA_00104 RTC Reverse Transcription Control
H08 N/A SA_00104 RTC Reverse Transcription Control
H09 N/A SA_00104 RTC Reverse Transcription Control
H10 N/A SA_00103 PPC Positive PCR Control
H11 N/A SA_00103 PPC Positive PCR Control
H12 N/A SA_00103 PPC Positive PCR Control

Related products
For optimal performance, RT² Profiler PCR Arrays should be used together with the RT² First Strand
Kit for cDNA synthesis and RT² SYBR®Green qPCR Mastermixes for PCR.
Product Contents Cat. no.
RT² First Strand Kit (12) Enzymes and reagents for cDNA synthesis 330401
RT² SYBR Green ROX™ FAST
Mastermix (2)*
For 2 x 96 assays in 96-well plates; suitable for use
with the Rotor-Gene Q and other Rotor-Gene cyclers
330620
* Larger kit sizes available; please inquire.
RT² Profiler PCR Array products are intended for molecular biology applications. These products are
not intended for the diagnosis, prevention, or treatment of a disease.
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit
handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.
com or can be requested from QIAGEN Technical Services or your local distributor.
Trademarks: QIAGEN®, Rotor-Gene®, Rotor-Disc™ (QIAGEN Group); ROX™ (Applera Corporation or its subsidiaries); SYBR®
(Molecular Probes, Inc.).
1067688 03/2011 © 2011 QIAGEN, all rights reserved.
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Sample & Assay Technologies