Qiagen QIAcube HT User manual

QIAcube HT
User Manual
For use with the QIAcube HT Prep Manager Software
Sample to Insight
December 2017

2QIAcube HT User Manual 12/2017
Contents
1..............................................................................................................1-2 Introduction
......................................................................................................1-2
1.1 Provided user manuals
......................................................................................................1-2
1.2 About this user manual
......................................................................................................1-3
1.3 General information
1.3.1 .......................................................................................1-3 Technical assistance
1.3.2 .......................................................................................1-3 Policy statement
1.3.3 .......................................................................................1-3 Version management
......................................................................................................1-3
1.4 Intended use of the QIAcube HT
......................................................................................................1-4
1.5 Requirements for QIAcube HT users
......................................................................................................1-4
1.6 Requirements for some kits
2..............................................................................................................2-2 Safety information
......................................................................................................2-2
2.1 Proper use
......................................................................................................2-4
2.2 Electrical safety
......................................................................................................2-5
2.3 Biological safety
2.3.1 .......................................................................................2-5 Samples
......................................................................................................2-6
2.4 Chemicals
2.4.1 .......................................................................................2-6 Toxic fumes
......................................................................................................2-6
2.5 Waste disposal
......................................................................................................2-6
2.6 Mechanical hazards
......................................................................................................2-7
2.7 Maintenance safety
......................................................................................................2-10
2.8 Symbols on the QIAcube HT
3..............................................................................................................3-2 General description
......................................................................................................3-3
3.1 QIAcube HT instrument
3.1.1 .......................................................................................3-4 Lid
3.1.2 .......................................................................................3-4 Feet
3.1.3 .......................................................................................3-5 Tip chute

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3.1.4 .......................................................................................3-5 Tip disposal box
3.1.5 .......................................................................................3-6 HEPA filter
3.1.6 .......................................................................................3-6 UV lamp
3.1.7 .......................................................................................3-7 Pipettor head and robotic arm
3.1.8 .......................................................................................3-7 Worktable
3.1.9 .......................................................................................3-9 Accessory panel
3.1.10 .......................................................................................3-10 Vacuum chamber
3.1.11 .......................................................................................3-12 Vacuum control station and waste bottle
3.1.12 .......................................................................................3-14 Pinch valve
3.1.13 .......................................................................................3-16 Internal features of the QIAcube HT
......................................................................................................3-18
3.2 Computer
4..............................................................................................................4-2 Installation procedures
......................................................................................................4-2
4.1 System delivery
......................................................................................................4-3
4.2 Requirements
4.2.1 .......................................................................................4-3 Installation site
4.2.2 .......................................................................................4-4 Power requirements
4.2.3 .......................................................................................4-4 Grounding requirements
......................................................................................................4-5
4.3 Unpacking and installation
4.3.1 .......................................................................................4-5 Unpacking the QIAcube HT
.............................................................................4-6
Releasing transport locks
4.3.2 .......................................................................................4-7 Installation of vacuum control station
4.3.3 .......................................................................................4-11 Installation of pinch valve
4.3.4 .......................................................................................4-11 Installation of tip disposal box holder
4.3.5 .......................................................................................4-12 UV lamp
4.3.6 .......................................................................................4-12 Installation of AC power cord
4.3.7 .......................................................................................4-13 Connecting the computer to the QIAcube HT
......................................................................................................4-14
4.4 Software installation
4.4.1 .......................................................................................4-14 Installing QIAcube HT Prep Manager Software
4.4.2 .......................................................................................4-15 Uninstalling QIAcube HT Prep Manager Software
4.4.3 .......................................................................................4-16 Updating QIAcube HT Prep Manager Software

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4.4.4 .......................................................................................4-17 Upgrading from legacy QIAcube HT Software
4.4.5 .......................................................................................4-18 Installing additional kits
5..............................................................................................................5-2 Getting started
......................................................................................................5-2
5.1 Start the software
......................................................................................................5-3
5.2 Customizing global settings
......................................................................................................5-7
5.3 Customize user settings
......................................................................................................5-9
5.4 Managing users
5.4.1 .......................................................................................5-15 Changing password
......................................................................................................5-16
5.5 Managing labware
......................................................................................................5-18
5.6 Assembling the vacuum chamber
5.6.1 .......................................................................................5-19 Assembly for calibration
5.6.2 .......................................................................................5-21 Assembly for standard purification protocols
......................................................................................................5-23
5.7 Instrument calibration
5.7.1 .......................................................................................5-25 Calibrate volumes
5.7.2 .......................................................................................5-27 Calibration positioning buttons
5.7.3 .......................................................................................5-28 Calibrate tip racks
5.7.4 .......................................................................................5-30 Calibrate labware
.............................................................................5-30
Select labware to calibrate
.............................................................................5-34
Prepare worktable for labware calibration
.............................................................................5-36
Calibrate X- and Y-axis
.............................................................................5-38
Calibrate Z-axis
5.7.5 .......................................................................................5-40 Saving and importing calibration data
6..............................................................................................................6-2 Operating procedures
......................................................................................................6-2
6.1 Starting a run
6.1.1 .......................................................................................6-3 Setup step
6.1.2 .......................................................................................6-5 Labware selection step
.............................................................................6-8
Select other input labware
6.1.3 .......................................................................................6-11 Assignment
.............................................................................6-12
Assigning samples

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.............................................................................6-13
Removing assignment
.............................................................................6-13
Blocking output positions
6.1.4 .......................................................................................6-13 Worktable step
.............................................................................6-16
Preparing the worktable
.............................................................................6-17
Checking the waste bottle status
.............................................................................6-18
Preparing tip chute and tip disposal box
.............................................................................6-20
Preparing the vacuum chamber
.............................................................................6-20
Loading tips
.............................................................................6-23
Loading reagents and samples
.............................................................................6-24
Starting a run
6.1.5 .......................................................................................6-24 Run step
.............................................................................6-25
Open system test
.............................................................................6-25
Plate detection test
.............................................................................6-26
Pausing/Stopping a run
.............................................................................6-27
Vacuum performance check
.............................................................................6-29
Run completion
......................................................................................................6-30
6.2 Finishing work and shutting down
7..............................................................................................................7-2 QIAcube HT Prep Manager Software
......................................................................................................7-2
7.1 Basic concepts and general software usage
7.1.1 .......................................................................................7-2 Concepts
.............................................................................7-2
User roles concept
.............................................................................7-3
Experiments and templates
.............................................................................7-5
Protection of files
7.1.2 .......................................................................................7-5 General software usage
.............................................................................7-5
Controlling the mouse
.............................................................................7-6
Use of color
.............................................................................7-7
Displaying errors and warnings
.............................................................................7-8
Entering data
.............................................................................7-10
Working with tables
.............................................................................7-11
Selecting samples

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7.1.3 .......................................................................................7-13 General elements
.............................................................................7-13
Menu
.............................................................................7-16
Main toolbar
.............................................................................7-18
Button bar
.............................................................................7-18
Status bar
.............................................................................7-19
File dialog
.............................................................................7-23
Scan plate barcode dialog
7.1.4 .......................................................................................7-23 Environments
.............................................................................7-24
Home
.............................................................................7-25
Experiments
.............................................................................7-27
Service
.............................................................................7-28
Configuration
7.1.5 .......................................................................................7-29 Getting help
......................................................................................................7-31
7.2 Working with experiments
7.2.1 .......................................................................................7-31 Manage experiments and templates
.............................................................................7-31
Create a template
.............................................................................7-32
Manage favorite templates
.............................................................................7-34
View recent experiments
7.2.2 .......................................................................................7-35 Creating an experiment
.............................................................................7-36
Create an empty experiment
.............................................................................7-37
Create an experiment using a template
.............................................................................7-39
Create an experiment from an existing
experiment
......................................................................................................7-39
7.3 Report
......................................................................................................7-44
7.4 Administrative tasks
7.4.1 .......................................................................................7-44 Configuring QIAcube HT
7.4.2 .......................................................................................7-44 Viewing software version
7.4.3 .......................................................................................7-44 View system audit trail
8..............................................................................................................8-2 Maintenance procedures
......................................................................................................8-2
8.1 Cleaning agents

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......................................................................................................8-4
8.2 Servicing
......................................................................................................8-4
8.3 Regular maintenance
8.3.1 .......................................................................................8-4 Cleaning the instrument after completing a run
8.3.2 .......................................................................................8-5 Cleaning the instrument before shutting down
8.3.3 .......................................................................................8-7 Cleaning the lid
8.3.4 .......................................................................................8-7 Emptying the waste bottle
8.3.5 .......................................................................................8-8 Cleaning accessories
8.3.6 .......................................................................................8-10 Using UV lamp
......................................................................................................8-11
8.4 Periodic maintenance
8.4.1 .......................................................................................8-11 Maintenance of the QIAcube HT instrument
8.4.2 .......................................................................................8-12 Maintenance schedule
8.4.3 .......................................................................................8-15 Maintenance of the QIAcube HT pipettor
8.4.4 .......................................................................................8-15 Replacing the HEPA filters
8.4.5 .......................................................................................8-18 Replacing filters on vacuum station
9..............................................................................................................9-2 Troubleshooting
......................................................................................................9-3
9.1 Support packages
......................................................................................................9-6
9.2 System setup
......................................................................................................9-9
9.3 Operation
......................................................................................................9-11
9.4 Self-diagnostic tools
9.4.1 .......................................................................................9-12 Connection check
9.4.2 .......................................................................................9-12 Cycle pipettor head
9.4.3 .......................................................................................9-13 Check for loss of steps
9.4.4 .......................................................................................9-13 Check vacuum pressure
9.4.5 .......................................................................................9-14 Check for home-opto alignment
10 ..............................................................................................................10-2 Glossary
11 ..............................................................................................................11-2 Appendices
......................................................................................................11-2
11.1 Appendix A
11.1.1 .......................................................................................11-2 Technical data
11.1.2 .......................................................................................11-2 Environmental conditions

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.............................................................................11-3
Waste Electrical and Electronical Equipment
11.1.3 .......................................................................................11-4 FCC declaration
11.1.4 .......................................................................................11-4 Declaration of conformity
......................................................................................................11-5
11.2 Appendix B
11.2.1 .......................................................................................11-5 Liability clause
......................................................................................................11-5
11.3 Appendix C Consignes de sécurité
11.3.1 .......................................................................................11-6 Utilisation appropriée
11.3.2 .......................................................................................11-8 Sécurité électrique
11.3.3 .......................................................................................11-9 Sécurité biologique
.............................................................................11-9
Échantillons
11.3.4 .......................................................................................11-10 Produits chimiques
.............................................................................11-10
Vapeurs toxiques
11.3.5 .......................................................................................11-10 Traitement des déchets
11.3.6 .......................................................................................11-11 Dangers mécaniques
11.3.7 .......................................................................................11-12 Sécurité relative à la maintenance
11.3.8 .......................................................................................11-14 Symboles sur le QIAcube HT
......................................................................................................11-15
11.4 Appendix D Sicherheitshinweise
11.4.1 .......................................................................................11-15 Sachgemäße Handhabung
11.4.2 .......................................................................................11-17 Elektrische Sicherheit
11.4.3 .......................................................................................11-18 Biologische Sicherheit
.............................................................................11-18
Proben
11.4.4 .......................................................................................11-18 Chemikalien
.............................................................................11-19
Giftige Dämpfe
11.4.5 .......................................................................................11-19 Abfallentsorgung
11.4.6 .......................................................................................11-19 Gefahren durch mechanische Teile
11.4.7 .......................................................................................11-20 Sicherheitshinweise – Wartungsarbeiten
11.4.8 .......................................................................................11-23 Symbole auf dem QIAcube HT
......................................................................................................11-24
11.5 Appendix E
11.5.1 .......................................................................................11-24 QIAcube HT Accessories
......................................................................................................11-26
11.6 Appendix F

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11.6.1 .......................................................................................11-26 License Terms
11.6.2 .......................................................................................11-63 Copyright information

Introduction
QIAcube HT User Manual 12/2017 1-1