Qiagen RT2 First Strand Kit User manual

RT² Profiler PCR Array (Rotor-Gene®Format)
Mouse Obesity
Cat. no. 330231 PAMM-017ZR
For pathway expression analysis
Format For use with the following real-time cyclers
RT² Profiler PCR Array,
Format R
Rotor-Gene Q, other Rotor-Gene cyclers
Description
The Mouse Obesity RT² Profiler PCR Array profiles the expression of 84 genes related to
obesity. The control of a relative stable body weight depends on a complex interaction
among the hormone axes in the peripheral nervous system and neurotransmitter signals in
the central nervous system that play a crucial role in maintaining a balanced energy
metabolism. This PCR Array includes obesity-related genes that are directly involved in the
regulation of energy intake and expenditure. The genes encode for orexigenic peptides,
hormones, and receptors; anorectic peptides, hormones, receptors; and central and
peripheral signaling molecules involved in energy expenditure. Using real-time PCR, you
can easily and reliably analyze expression of a focused panel of genes related to Obesity
with this array.
For further details, consult the RT² Profiler PCR Array Handbook.
Shipping and storage
RT² Profiler PCR Arrays in the Rotor-Gene format are shipped at ambient temperature, on
dry ice, or blue ice packs depending on destination and accompanying products.
For long term storage, keep plates at –20°C.
Note: Ensure that you have the correct RT² Profiler PCR Array format for your real-time
cycler (see table above).
Note: Open the package and store the products appropriately immediately on receipt.
Sample & Assay Technologies

Array layout
The 96 real-time assays in the Rotor-Gene format are located in wells 1–96 of the Rotor-Disc™ (plate
A1–A12=Rotor-Disc 1–12, plate B1–B12=Rotor-Disc 13–24, etc.). To maintain data analysis
compatibility, wells 97–100 do not contain real-time assays but will contain master mix to account
for weight balance.
Gene table: RT² Profiler PCR Array
Position UniGene GenBank Symbol Description
A01 Mm.3407 NM_009625 Adcyap1 Adenylate cyclase activating polypeptide 1
A02 Mm.44245 NM_007407 Adcyap1r1 Adenylate cyclase activating polypeptide 1 receptor 1
A03 Mm.3969 NM_009605 Adipoq Adiponectin, C1Q and collagen domain containing
A04 Mm.259976 NM_028320 Adipor1 Adiponectin receptor 1
A05 Mm.291826 NM_197985 Adipor2 Adiponectin receptor 2
A06 Mm.347390 NM_009633 Adra2b Adrenergic receptor, alpha 2b
A07 Mm.46797 NM_007419 Adrb1 Adrenergic receptor, beta 1
A08 Mm.441696 NM_007427 Agrp Agouti related protein
A09 Mm.4533 NM_007468 Apoa4 Apolipoprotein A-IV
A10 Mm.119936 NM_009730 Atrn Attractin
A11 Mm.1442 NM_007540 Bdnf Brain derived neurotrophic factor
A12 Mm.10687 NM_009766 Brs3 Bombesin-like receptor 3
B01 Mm.19131 NM_009778 C3 Complement component 3
B02 Mm.4361 NM_007587 Calca Calcitonin/calcitonin-related polypeptide, alpha
B03 Mm.4642 NM_007588 Calcr Calcitonin receptor
B04 Mm.75498 NM_013732 Cartpt CART prepropeptide
B05 Mm.2619 NM_031161 Cck Cholecystokinin
B06 Mm.3521 NM_009827 Cckar Cholecystokinin A receptor
B07 Mm.21160 NM_025469 Clps Colipase, pancreatic
B08 Mm.7992 NM_007726 Cnr1 Cannabinoid receptor 1 (brain)
B09 Mm.425178 NM_016673 Cntfr Ciliary neurotrophic factor receptor
B10 Mm.276736 NM_007754 Cpd Carboxypeptidase D
B11 Mm.31395 NM_013494 Cpe Carboxypeptidase E
B12 Mm.1892 NM_007762 Crhr1 Corticotropin releasing hormone receptor 1
C01 Mm.54161 NM_010076 Drd1a Dopamine receptor D1A
C02 Mm.41970 NM_010077 Drd2 Dopamine receptor D2
C03 Mm.4655 NM_010253 Gal Galanin
C04 Mm.6219 NM_008082 Galr1 Galanin receptor 1
C05 Mm.45494 NM_008100 Gcg Glucagon
C06 Mm.22329 NM_008101 Gcgr Glucagon receptor
C07 Mm.343934 NM_008117 Gh Growth hormone
C08 Mm.3986 NM_010284 Ghr Growth hormone receptor
C09 Mm.379095 NM_021488 Ghrl Ghrelin
C10 Mm.194721 NM_177330 Ghsr Growth hormone secretagogue receptor
C11 Mm.390969 NM_021332 Glp1r Glucagon-like peptide 1 receptor
C12 Mm.20298 NM_175012 Grp Gastrin releasing peptide
D01 Mm.4687 NM_008177 Grpr Gastrin releasing peptide receptor
D02 Mm.10096 NM_010410 Hcrt Hypocretin
D03 Mm.246595 NM_198959 Hcrtr1 Hypocretin (orexin) receptor 1
D04 Mm.333327 NM_008285 Hrh1 Histamine receptor H1
D05 Mm.439670 NM_008312 Htr2c 5-hydroxytryptamine (serotonin) receptor 2C
D06 Mm.415 NM_010491 Iapp Islet amyloid polypeptide
D07 Mm.15534 NM_010554 Il1a Interleukin 1 alpha
D08 Mm.222830 NM_008361 Il1b Interleukin 1 beta
D09 Mm.896 NM_008362 Il1r1 Interleukin 1 receptor, type I
D10 Mm.1019 NM_031168 Il6 Interleukin 6
D11 Mm.2856 NM_010559 Il6ra Interleukin 6 receptor, alpha
D12 Mm.46269 NM_008386 Ins1 Insulin I
E01 Mm.4946 NM_008387 Ins2 Insulin II
E02 Mm.268003 NM_010568 Insr Insulin receptor
E03 Mm.277072 NM_008493 Lep Leptin
E04 Mm.259282 NM_010704 Lepr Leptin receptor
E05 Mm.57183 NM_008561 Mc3r Melanocortin 3 receptor
E06 Mm.323523 NM_145132 Mchr1 Melanin-concentrating hormone receptor 1
E07 Mm.22246 NM_026523 Nmb Neuromedin B
E08 Mm.425622 NM_008703 Nmbr Neuromedin B receptor
E09 Mm.154879 NM_019515 Nmu Neuromedin U

Position UniGene GenBank Symbol Description
E10 Mm.389159 NM_010341 Nmur1 Neuromedin U receptor 1
E11 Mm.154796 NM_023456 Npy Neuropeptide Y
E12 Mm.5112 NM_010934 Npy1r Neuropeptide Y receptor Y1
F01 Mm.129481 NM_008173 Nr3c1 Nuclear receptor subfamily 3, group C, member 1
F02 Mm.130054 NM_008745 Ntrk2 Neurotrophic tyrosine kinase, receptor, type 2
F03 Mm.64201 NM_024435 Nts Neurotensin
F04 Mm.301712 NM_018766 Ntsr1 Neurotensin receptor 1
F05 Mm.7977 NM_011011 Oprk1 Opioid receptor, kappa 1
F06 Mm.457998 NM_001039652 Oprm1 Opioid receptor, mu 1
F07 Mm.277996 NM_008895 Pomc Pro-opiomelanocortin-alpha
F08 Mm.212789 NM_011144 Ppara Peroxisome proliferator activated receptor alpha
F09 Mm.3020 NM_011146 Pparg Peroxisome proliferator activated receptor gamma
F10 Mm.259072 NM_008904 Ppargc1a Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha
F11 Mm.377241 NM_201615 Prlhr Prolactin releasing hormone receptor
F12 Mm.277916 NM_011201 Ptpn1 Protein tyrosine phosphatase, non-receptor type 1
G01 Mm.46248 NM_145435 Pyy Peptide YY
G02 Mm.39884 NM_019511 Ramp3 Receptor (calcitonin) activity modifying protein 3
G03 Mm.425181 NM_011014 Sigmar1 Sigma non-opioid intracellular receptor 1
G04 Mm.157119 NM_019972 Sort1 Sortilin 1
G05 Mm.2453 NM_009215 Sst Somatostatin
G06 Mm.454968 NM_009217 Sstr2 Somatostatin receptor 2
G07 Mm.32563 NM_009380 Thrb Thyroid hormone receptor beta
G08 Mm.1293 NM_013693 Tnf Tumor necrosis factor
G09 Mm.1363 NM_009426 Trh Thyrotropin releasing hormone
G10 Mm.377116 NM_021290 Ucn Urocortin
G11 Mm.4177 NM_009463 Ucp1 Uncoupling protein 1 (mitochondrial, proton carrier)
G12 Mm.290924 NM_053009 Zfp91 Zinc finger protein 91
H01 Mm.328431 NM_007393 Actb Actin, beta
H02 Mm.163 NM_009735 B2m Beta-2 microglobulin
H03 Mm.343110 NM_008084 Gapdh Glyceraldehyde-3-phosphate dehydrogenase
H04 Mm.3317 NM_010368 Gusb Glucuronidase, beta
H05 Mm.2180 NM_008302 Hsp90ab1 Heat shock protein 90 alpha (cytosolic), class B member 1
H06 N/A SA_00106 MGDC Mouse Genomic DNA Contamination
H07 N/A SA_00104 RTC Reverse Transcription Control
H08 N/A SA_00104 RTC Reverse Transcription Control
H09 N/A SA_00104 RTC Reverse Transcription Control
H10 N/A SA_00103 PPC Positive PCR Control
H11 N/A SA_00103 PPC Positive PCR Control
H12 N/A SA_00103 PPC Positive PCR Control

Related products
For optimal performance, RT² Profiler PCR Arrays should be used together with the RT² First Strand
Kit for cDNA synthesis and RT² SYBR®Green qPCR Mastermixes for PCR.
Product Contents Cat. no.
RT² First Strand Kit (12) Enzymes and reagents for cDNA synthesis 330401
RT² SYBR Green ROX™ FAST
Mastermix (2)*
For 2 x 96 assays in 96-well plates; suitable for use
with the Rotor-Gene Q and other Rotor-Gene cyclers
330620
* Larger kit sizes available; please inquire.
RT² Profiler PCR Array products are intended for molecular biology applications. These products are
not intended for the diagnosis, prevention, or treatment of a disease.
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit
handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.
com or can be requested from QIAGEN Technical Services or your local distributor.
Trademarks: QIAGEN®, Rotor-Gene®, Rotor-Disc™ (QIAGEN Group); ROX™ (Applera Corporation or its subsidiaries); SYBR®
(Molecular Probes, Inc.).
1067688 03/2011 © 2011 QIAGEN, all rights reserved.
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Sample & Assay Technologies