Quidel Lyme FIA Sofia 2 User manual

QuickStart Guide
CUSTOMER SERVICE
If Sofia 2 does not perform as expected, contact Quidel Technical Support 800.874.1517 (in the U.S.),
858.552.1100 (outside the U.S.), technicalsuppo[email protected], or your local distributor.
Firmware v.1.12 or higher
Study the User Manual thoroughly before using the QuickStart Guide.
The User Manual can be accessed online at
http://www.quidel.com/immunoassays/sofia-tests-kits/sofia-2-analyzer

UNPACK SOFIA 2
Inspect the shipping container for obvious shipping damage prior to opening.
Unpack the shipping container and inspect the unit for damage.
CONNECT TO POWER AND TURN ON
Place Sofia 2 on the countertop within reach of an electrical outlet. The unit is portable and can be
moved to a suitable location for testing. If you wish to take advantage of the new “Connect to Quidel”
features, please ensure the Sofia 2 has access to Wi-Fi or an ethernet port (See page 3 for more details).
Plug the Power Adapter into the power port on the rear
panel of Sofia 2 and the appropriate electrical outlet.
Turn Sofia 2 on using the power switch located on the rear
panel. A Power-On Self-Test screen will appear on the front
display when Sofia 2 is turned on.
Power Port
Power Switch
1
2

After a Firmware update, or when turning on Sofia 2 for the first time, the User may see several screens
upon startup designed to personalize Sofia 2 to their setting.
ENTER ZIP CODE
Sofia 2 will prompt the User to enter the ZIP Code where it is located. Select to confirm and continue
to the next screen.
Confirm Time and Date Settings
After a Firmware update, Sofia 2 will prompt the User to verify that the time and date displayed are
correct. Press to go to the Time and Date screen pictured below and verify the time settings or
change them if necessary. Press to complete.
Connect to Quidel
Users will be prompted to set their software update settings.
The “Download from Quidel” options enable Sofia 2 to securely and automatically download software
updates directly from the cloud via a Wired Ethernet or Wi-Fi connection. To Connect to Quidel, Users
will select their preferred installation method under “Download from Quidel” and press .

If the Sofia 2 is already connected to the internet the User will receive confirmation of successful
registration and press to complete.
Users who choose not to Connect to Quidel will select “Install updates manually with a USB drive” and
press to complete.
Users who want their Sofia 2 to be Connected to Quidel, but whose instruments are not yet connected
to the internet please follow the steps below.
After selecting the preferred “Download from Quidel” option, press . The Sofia 2 will prompt the User
to set up their network.
Select Wired Ethernet or Wi-Fi.
Wired Ethernet: For Set IP Address, select Manual or DHCP.
If DHCP is selected, select to test the connection and complete the network setup.
If Manual is selected, press to continue to the next screen. Using the on-screen keypad, enter
information for IP Address, Subnet Mask, and Gateway, then press . On the next screen, enter

information for Primary and Secondary DNS. Press to test the connection and complete the network
setup.
Wi-Fi: Select your Wi-Fi network from the list of available options while considering each network’s
signal strength. Enter the Wi-Fi Password and press to test the connection and complete the network
setup.

TEST VIRENA CONNECTION
The next step is to test your Virena Connection. From the Main Menu, select Send Data. Then select
Test connection and press to test the Virena connection.
If the connection is successful, the message Test connection success will appear with additional
information detailing the type of Virena connection. Sofia 2 is now connected to the Virena cloud. If the
connection is unsuccessful, contact Technical Support.

If “Test Virena Connection” was a success, skip to Run Calibration on the next page.
CUSTOMERS WITHOUT VIRENA ACTIVATED
For Sofia 2 customers without Virena activated, testing the connection will result in the message Send
To Virena Error. Unable to Acquire Cellular Connection or the Test Connection pop-up window could
continue for several minutes requiring the User to restart the instrument.
To prevent this, select Supervisor Menu from the Main Menu. Then select Network Settings, then
Virena Settings. Configure settings to the following:
Press to confirm changes and complete the Virena setup.

RUN CALIBRATION
Enter the default Supervisor ID of 1234.
Remove Calibration Cassette from the pouch.
Press to advance to the next screen.
Following the prompts, insert the Calibration Cassette into Sofia 2 and gently close the drawer. Sofia 2
will perform the Calibration Check automatically.
The Sofia 2 display will indicate when the Calibration Check is completed.
NOTE: If Calibration Check cannot be completed successfully, notify the on-site Supervisor or contact
Quidel Technical Support for assistance.
Once calibration is complete, remove and return the
Calibration Cassette to the storage pouch.
Important: Store the Calibration Cassette in the
provided storage pouch between uses. Avoid
touching the read window on the Calibration
Cassette.
Run QC according to assay-specific Package Insert.
Default User ID: 1234
The Calibration Cassette provided
with the instrument should be
reused until the expiration date
printed on the pouch. Please do
not discard the Calibration
Cassette until the expiration date.

MENU STRUCTURE
1330304EN00 (05/21)
Quidel Corporation
San Diego, CA 92121 USA
quidel.com