Rainin E-Man Hybrid User manual

Getting Started with E-Man Hybrid™
Figure 1 E-Man Hybrid Controls and Indicators
LCD
Battery
Tray
(beneath display)
CC Key
MODE Key
Plunger Button
Volume Lock
Lever
Tip Ejector
Button
Activate the Battery
E-Man Hybrid is shipped with a plastic strip isolating the battery ter-
minal. Remove this strip by pulling straight out from the battery
tray. Press the plunger button to begin operation and clear “PrES”.
Set the Volume
IUnlock the volume lock by moving the lever counterclockwise.
ISet the desired volume by rotating the plunger button. The dis-
play changes as you rotate the button.
IStop at the desired volume and lock the volume lock to operate.
Display Modes Press the MODE and CC keys for these modes.
MODE KEY: Scrolls through these three display modes.
Pipet Mode:Same operation as standard pipettes, see over.
Track Mode: TRACK is displayed. The volume of sample in the
tip is shown in real time as the plunger button is depressed.
When pressed beyond the 1ST STOP position the display reads
“bLo” to indicate the Blowout part of the stroke.
Titrate Mode: TITRATE is displayed. The LCD shows the volume
of sample dispensed from the tip in real time, as a negative num-
ber relative to the upper set volume (at the set volume the LCD
shows 0.0).
CC KEY: Turns on or off the cycle counter, and clears the count.
Cycle Counter: Cycle count is a visual reference of how many
true pipetting cycles have occurred in the current pipetting ses-
sion. CYCLES and the count number are shown in the display.
Figure 2 Volume Lock
9920-349 E-MAN_QRG 8/28/07 2:49 PM Page 1

RAININ and LTS are registered trademarks and E-Man is a trademark of Rainin Instru-
ment, LLC. E-Man Hybrid Pipette: U.S. Patent Nos. 5,614,153; 5,849,248. Other
patents pending. LTS Tip/Shaft System: For use under U.S. Patent Nos. 6,168,761
B1; 6,171,553 B1; 6,568,288 B2; 6,737,023 B1; 6,745,636 B2; 6,967,004 B2
Operation in PIPET Mode
Before pipetting valuable samples, it is a good idea to practice
aspirating and dispensing water.
Go to PIPET mode by pressing the MODE key
1. Unlock the volume lock and set the desired volume. Lock the
volume lock lever to begin measurements. When locked, the
volume display will not change, just like in a standard pipette
2. Attach a new RAININ tip by pressing the shaft into the tip with
sufficient force to make a good seal. For E-Man Hybrid LTS
models, press until the positive stop is reached.
3. Press the plunger button to the first
stop. The word PICKUP is shown on
the display as an aid to locate the
HOME position. Hold the plunger
button in this position.
4. Holding E-Man Hybrid vertically, place the tip into the sample
and relax thumb pressure on the plunger button. The light pis-
ton spring will move the piston upward, aspirating sample. Do
not let go of the plunger button, or the piston may snap up
quickly, resulting in inaccurate measurement.
5. Pause briefly with the tip end still in the sample to ensure that
the full volume of sample is drawn into the tip.
6. Withdraw the tip from the sample. If any liquid remains on the
outside of the tip, wipe it carefully with a lint-free tissue, taking
care not to touch the tip orifice.
Dispensing:
1. Touch the tip end against the side wall of the receiving vessel
and press the plunger slowly, past the first stop (PICKUP will
appear on the LCD as you reach the 1ST STOP) to blowout (bot-
tom of stroke.) Wait 1 second for volumes to 300 μL, 1-2 sec-
onds for 1000 μL and larger.
2. Still holding the plunger button, withdraw the tip, sliding it along
the wall of the vessel.
3. Release the plunger button and press the tip ejector button light-
ly to discard the tip. Use a new tip for each sample to prevent
carry-over. Repeat for the next pipetting cycle.
Sleep Mode
After 3 minutes of no activity the display will go to sleep to conserve
battery life. To operate the pipette again simply touch any key or
press the plunger button through the 1ST STOP.
Read the Manual
See the manual for detailed information on E-Man Hybrid operation.
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