Rocker BioVac 225 User manual

BioVac 225
BioVac 225 Plus
BioVac 225 - BioDolphin
Portable suction system

A. Unpacking
When unpacking, notice if the Bio-Suction System is in good situation. If not, please contact the distributor or
contact us by e-mail: [e-mail] immediately for assistance.
Model BioVac 225 BioVac 225 Plus BioVac 225 - BioDolphin
Standard
Package
Includes:
BioVac 225 suction unit
Suction bottle
Disc filter (PTFE)
Tube connecting adaptor
2m Silicone tube
1-channel tip adaptor (150
mm)
Instruction manual
x1
x1
x1
x1
x1
x1
x1
BioVac 225 suction unit
Suction bottle
Disc filter (PTFE)
Tube connecting adaptor
2m Silicone tube
Handle
8 channel tip adaptor with
ejector
1 channel tip adaptor with
ejector
1-channel tip adaptor (150
mm)
Repair kit of handle
Instruction manual
x1
x1
x1
x1
x1
x1
x1
x1
x1
x1
x1
BioVac 225 suction unit
Suction bottle
Disc filter (PTFE)
Tube connecting adaptor
2m Silicone tube
1 channel tip adaptor
1 channel S.S. adaptor
(40mm)
BioDolphin Suction Kit
Instruction manual
x1
x1
x1
x1
x1
x1
x1
x1
x1
B. Important Notice
Before using the instrument, please ensure that the power supply voltage matches the voltage rating for
the instrument.
Please ensure that all connections are well-sealed.
The disc filter needs to be replaced periodically or immediately once it is contaminated or saturated.
The built-in thermal protector will shut down the instrument automatically when it gets too hot.
Please check the fuse, if the instrument doesn't work and the switch light doesn't light up after powered
on.
Do not use the instrument with organic or corrosive gases as it is not a chemical resistant product.
Install the instrument in a clean, dust-less and ventilated area under 40 ℃.
Pour some water into the suction bottle before use for easy cleaning after use. Clean the suction bottle
with only tap water instead of any chemical detergents to prevent corrosion.
It is always recommended to pour out waste solution in the suction bottle upon reaching 2/3 of the full
level to prevent overflowing into the pump.
Avoid letting the instrument fall down to prevent the damage. If you have any problems in the instrument,
please contact your local distributor for assistance immediately.

C. Portable Suction System Diagram
<pic 1> BioVac 225
<pic 2> BioVac 225 Plus
<pic 3> BioVac 225 - BioDolphin

D. Assembly & Disassembly diagram
Item Description
1 BioVac 225 suction unit
2 Disc filter
3 Suction bottle
4 Tube connection adaptor
5 2m silicone tube
6 1-channel tip adaptor (150 mm)
<pic 4> BioVac 225
<pic 5> BioVac 225 Plus
<pic 6> BioVac 225 - BioDolphin
Item Description
1 BioVac 225 suction unit
2 Disc filter
3 Suction bottle
4 Tube connection adaptor
5 2m silicone tube
6 Handle
7 1 channel tip adaptor with ejector
8 8 channel tip adaptor with ejector
9 1-channel tip adaptor (150 mm)
10 Repair kit of handle
Item Description
1 BioVac 225 suction unit
2 Disc filter
3 Suction bottle
4 Tube connection adaptor
5 2m silicone tube
6 BioDolphin suction kit
7 Repair kit of handle

Item Description
1 Power cable
2 Fuse sleeve & Fuse
3 On/Off switch
4 Vacuum indicator
5 Vacuum regulator
6 Vacuum pump inlet
7 Suction bottle outlet
8 Suction bottle inlet
<pic 7> BioVac 225 suction unit and suction bottle part list
<pic 8> Overflow protection
Item Description
1Overflow protection

item Description
1 Handle Head
2 Lock button
3 Suction button
4 Handle end
<pic 9> Handle part list
<pic 10> Handle Disassembly
<pic 11> Operating of 8 channel tip adaptor with ejector <pic 12> Change different tip adaptor
item Description
1 Rubber adaptor
2 O-ring AS004
3 O-ring P7
4 Rubber valve
5 S.S. spring
6 O-ring AS012

E. Operating Procedure
1. BioVac 225 <pic 1>
a. Ensure that the overflow protection is installed properly then place the suction bottle on the holder.
Please refer to <pic 9> when installing it.
Note: please always put the overflow protection into the waste bottle cover gently to avoid
the deformation of its plastic tube that could cause a malfunction.
b. Connect disc filter with tubes according to <pic 4>.
c. Connect the tube connecting adaptor to both thin, thick silicone tube and suction bottle as <pic 4>
then select a desired fitting part to be connected with the thin silicone tube.
d. Plug the power cord into a proper power supply then press power switch to turn on the machine.
After blocking the fitting part with a hand, adjust vacuum level to a desired value by turning the
vacuum regulator then proceed with suction operation.
e. The 1-channel tip adaptor (150mm) is recommended to be plugged into the handle then have it
connect with 200 µl disposable tip (the other side can connect with 1µl disposable tip ) with
which to prevent cross contamination in common microplate and culture dish.
f. The complete set of BioDolphin suction kit (199100-00) can cater for various kinds of waste liquid
container and suction.
g. After the process is finished then turn off the machine. Dispose of the contents in the waste bottle
in accordance with the applicable regulations.
h. Proceed with cleaning and maintenance after every use.
Note: Check the liquid level of the waste bottle before each use and then empty it as per the
applicable regulations.
2. BioVac 225 Plus <pic 2>
a. Ensure that the overflow protection is installed properly then place the suction bottle on the holder.
Please refer to <pic 9> when installing it.
Note: please always put the overflow protection into the suction bottle cover gently to avoid
the deformation of its plastic tube that could cause a malfunction.
b. Install the complete system according to <pic 5>.
c. Plug the power cord into a proper power supply then press power switch to turn on the machine.
After blocking the handle with a hand, adjust vacuum level to a desired value by turning the
vacuum regulator then proceed with suction operation.
d. Ensure that air comes into the front port once pressing the suction button on the handle before
proceeding with suction operation.
e. The 1-channel S.S. adaptor (40 mm) can be plugged into handle directly for waste suction of
microplate or culture dish by pressing the suction button or pressing lock button to keep a
continuous operation.
f. The 1-channel tip adaptor is recommended to be plugged into the handle then have it connect
with 200 µl disposable tip (the other side can connect with 1µl disposable tip ) with which to
prevent cross contamination in common microplate and culture dish.
g. For 96-well microplate ELISA test, the 8-channel tip connector is recommended to be plugged into

the handle and have it insert the tip box to take in 8 tips at one time; remove the used tips by
pressing ejection button and throw them into collection bin after suction; connect with new tips to
repeat suction operation; press lock button to keep a long-time operation if needed.
h. After the process is finished then turn off the machine; Proceed with cleaning and maintenance
after every use according to <F. Maintenance>.
Note: Check the liquid level of the waste bottle before each use and then empty it as per the
applicable regulations.
3. BioVac 225 - BioDolphin <pic 3>
a. Ensure that the overflow protection is installed properly then place the suction bottle on the holder.
Please refer to <pic 9> when installing it.
Note: please always put the overflow protection into the suction bottle cover gently to avoid
the deformation of its plastic tube that could cause a malfunction.
b. Install the complete system of all parts according to <pic 6>
c. Plug the power cord into a proper power supply then press power switch to turn on the machine.
After blocking the handle with a hand, adjust vacuum level to a desired value by turning the
vacuum regulator then proceed with suction operation.
d. Ensure that air comes into the front port once pressing the suction button of the handle before
proceeding with suction operation.
e. The 1-channel S.S. adaptor (40 mm) can be plugged into handle directly for waste suction of
microplate or culture dish by pressing the suction button or pressing lock button to keep a
continuous operation.
f. The 1-channel tip adaptor is recommended to be plugged into the handle then have it connect with
200 µl disposable tip with which to prevent cross contamination in common microplate and culture
dish.
g. For 96-well microplate ELISA test, the 8-channel S.S. adaptor (40 mm) is recommended to be
plugged into the handle and press suction button to draw waste in 8 wells at one time; press lock
button to keep continuous operation if needed.
h. The 8-channel tip connector is recommended to be plugged into the handle and have it insert the
tip box to take in 8 tips at one time; remove the used tips by pressing ejection button and throw
them into collection bin after suction; connect with new tips to repeat suction operation; press lock
button to keep a continuous operation if needed.
i. For deeper containers, the 1-channel S.S. adaptor (80 mm) is recommended to be plugged into
the handle and put in into the container then press suction button to draw waste; press lock button
to keep a continuous operation if needed.
j. If the 1-channel S.S. adaptor (80 mm) is not long enough then replace it with glass or plastic
pipette.
k. After the process is finished then turn off the machine; Proceed with cleaning and maintenance
after every use according to <E. Maintenance>
Note: Check the liquid level of the waste bottle before each use and then empty it as per the
applicable regulations.

F. Maintenance
1. After each use, please rinse suction bottle with clean water rather than any chemical detergents which
may cause cracks or damage to the structure of the suction bottle.
2. Rinse the internal part of silicone tube、tube connecting adaptor、handle and all fitting adaptors by
sucking 30cc water then sterilize with an autoclave and wipe with a dry cloth after each use.
3. This bottle is made of PC material and available for autoclave at 121°C for 30 min.
4. The rack and liquid collection tray are not available for autoclave; please clean with water and sterilize
with 75% alcohol.
5. If SS tube is blocked, please soak it in water for 30 min. and clean the blocked tube with L type cleaning
rod.
6. It is strongly recommended to replace internal rubber adaptor、O-ring and SS spring of the handle with
new parts every year at least to keep good air tightness. Please replace with new parts by referring to
<pic 10>.
Note: O-ring kit (199100-53) available for order
7. Disc filter and silicone tubes are consumables, please replace at least every half year to ensure sufficient
air flow.
G. Troubleshooting
Symptoms Possible causes and Solution
Device fails to start
1. Check the power supply conform to power specified on main unit
2. Blown fusereplace fuse if it is burnt out
3. Check if the power switch is in the ON position
4. Vacuum still exists in systemrelease vacuum and restart
5. Defective switchcontact your distributor for repair
Vacuum fails to adjust
1. Faulty gaugecontact your distributor for repair
2. Loose regulatortighten the regulator by yourself or contact your
distributor for repair
3. Faulty regulator contact your distributor for repair
Low filtration speed
1. Regulator improperly setset regulator vacuum level
following instruction manual
2. Air leakCheck the tubing and connected accessories for possible
leaks. (Attach, tighten or replace)
3. Check if the suction bottle is overflowing
4. Check if the PTFE Disc filter is good part
Weak aspiration
1. Adjust vacuum to proper level or change vacuum source
2. Air leakage from tubing change silicone tube
3. Air leakage form handle change rubber adaptor or O-ring
4. Handle or adaptor is broken contact your distributor for repair

H. Ordering Information
167225-11 BioVac 225 suction system , AC110V / 60Hz
167225-22 BioVac 225 suction system , AC220V/50Hz
167226-11 BioVac 225 –BioDolphin(light blue) suction system, AC110V/60Hz
167226-22 BioVac 225 – BioDolphin(light blue) suction system, AC220V/50Hz
167227-11 BioVac 225 Plus suction system, AC110V / 60Hz
167227-22 BioVac 225 Plus suction system, AC220V / 50Hz
199100-00 BioDolphin-LB, Suction kit –light blue
199110-00 BioDolphin-DB, Suction kit –deep blue
199120-00 BioDolphin-P, Suction kit –pink
199130-00 BioDolphin-G, Suction kit –green
199100-30 Handle
199100-46 1 channel tip adaptor
199100-47 1 channel S.S. adaptor (40 mm)
199100-48 1 channel S.S. adaptor (80 mm)
199100-49 8 channel S.S. adaptor (40 mm)
199100-50 8 channel tip adaptor with ejector
199100-75 1 channel tip adaptor with ejector
199100-76 1-channel tip adaptor (150 mm)
199100-61 Rack
199100-51 Repair kit of handle (include L bar, rubber adaptor, S.S. spring)
199100-68 Silicone tube (Ф4.2 mm x Ф8 mm ), 200 cm
199100-53 O-ring kit (include O-ring P7, O-ring AS004, O-ring AS012, rubber adaptor, rubber valve)
Rocker Scientific Co., Ltd. / www.rocker.com.tw / EnV2016.05