Sartorius Proline User manual

User Manual
Bedienungsanleitung
Mode d'emploi
Manual Usuario
Instruzioni d'impiego
Инструкция пользователя
Proline®pipette

User Manual ............................................................1
Bedienungsanleitung .......................................... 15
Mode d’ emploi ..................................................... 29
Manual Usario ....................................................... 43
Instruzioni d’impiego .......................................... 57
Инструкция пользователя ................................. 73
Specifications ........................................................ 88
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Proline®pipette

1
Contents
1. Intended use .....................................................................................................2
2. Product description........................................................................................2
2.1. Sartorius Proline adjustable volume pipettes ....................... 2
2.2. Sartorius Proline fixed volume pipettes.................................. 3
2.3. Sartorius pipette tips......................................................................3
3. Unpacking.......................................................................................................... 4
4. Installing the pipette holder....................................................................... 4
5. Pipette Materials.............................................................................................5
6. Operating the pipette....................................................................................6
6.1. Volume setting..................................................................................6
6.2. Sealing and ejecting tips...............................................................6
6.3. Two-in-one pipettes .......................................................................6
6.4. Protective filters ..............................................................................7
7. Pipetting techniques...................................................................................... 7
7.1. Forward pipetting............................................................................7
7.2. Reverse pipetting.............................................................................8
8. Storage................................................................................................................8
9. Testing the performance and recalibration ..........................................8
9.1. Testing the performance............................................................... 9
9.2. Recalibration procedure..............................................................11
10. Maintenance...................................................................................................11
10.1. Daily cleaning of the outer surface of the pipette ...........11
10.2. In-house maintenance.................................................................12
11. Troubleshooting.............................................................................................13
12. Warranty information.................................................................................14

2
1. Intended use
Proline pipette is intended to be used in liquid
handling applications for dispensing liquids
in total volume range from 0,1 µl to 5 ml. It is
recommended to use Optifit Tips or SafetySpace™
Filter tips to ensure the best compatibility and
performance with Sartorius pipettes.
This liquid handling device is designed and
manufactured to be used as general purpose
laboratory equipment. Before operating it is
recommended to read the user manual, which
contains useful information e.g. for good
pipetting practice.
2. Product description
Your new Proline hand-held pipette is a general
purpose pipette, for the accurate and precise
sampling and dispensing of liquid volumes. All
Proline pipettes operate on the basis of the air
displacement principle and use disposable tips.
The range of Sartorius Proline pipettes cover a
volume range from 0.1 µl to 5 ml.
2.1. Sartorius Proline adjustable
volume pipettes
Single channel pipettes:
Cat. No. Volume
Range/µl
Increment /
µl
Tip/
µl
Safe-Cone Filters
50 pcs/bag
Standard Plus
720010 0.1-2.5 0.05 10 - -
720015 0.5-10 0.1 10, 200 - -
720080 2-20 0.5 200 721008 721018
720025 5-50 0.5 200, 350 721008 721018
720050 10-100 1200, 350 721007 721017
720070 20-200 1200, 350 721007 721017
720060 100-1000 51000 721006 721016
720110 1000-5000 50 5000 721005 721015

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Multichannel pipettes:
2.2. Sartorius Proline fixed
volume pipettes
Single channel pipettes:
2.3. Sartorius pipette tips
Sartorius pipette tips are recommended for use
with Proline® Plus pipettes. The use of fully
compatible tips will ensure the maximal pipetting
accuracy and precision, and thereby guarantee
the performance specifications given for the
pipette. Sartorius tips are made of pure virgin
polypropylene and manufactured in protected
clean room conditions.
Sartorius offers a full range of standard Optifit,
and SafetySpace™ Filter tips. Sartorius non-filter
tips are available in trays, bulk packages and
space-saving refill systems. Sartorius non-filtered
tips and trays are autoclavable at 121°C (252F),
Cat. No. Volume Range/
µl
Increment /
µl
Tip/
µl
Safe-Cone Filters
50 pcs/bag
Standard
720210 8-ch 0.50-10 01 10 -
720220 8-ch 5-50 0.5 200, 350 721014
720240 8-ch 50-300 5350 721014
720310 12-ch 5-10 0.1 10 -
720320 12-ch 5-50 0.5 200, 350 721014
720340 12-ch 50-300 5350 721014
Cat. No. Volume
Range/µl
Tip/
µl
Safe-Cone Filters
50 pcs/bag
Standard Plus
722001 510, 200 - -
722004 10 10, 200 - -
722010 20 200, 350 721008 721018
722015 25 200, 350 721008 721018
722020 50 200, 350 721008 721018
722025 100 200, 350 721007 721017
722030 200 200, 350 721007 721017
722035 250 1000 721006 721016
722040 500 1000 721006 721016
722045 1000 1000 721006 721016
722050 2000 5000 721005 721015
722055 5000 5000 721005 721015

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20 min, 1 bar (15 p.s.i). All Single Tray Racks and
clean Refill Packs are certified RNase, DNase and
endotoxin-free.
For more information, please go to www.sartorius.
com or contact your local Sartorius support.
3. Unpacking
The pipette package contains the following items:
1. Pipette
2. Calibration/Opening tool
3. Grease
4. Instructions for use
5. Pipette holder
6. Tip
7. Filters (pipettes > 10 µl)
8. Performance certificate according to ISO
8655-6
4. Installing the pipette
holder
For convenience and safety always keep the
pipette vertically on its own holder when not in
use. When installing the holder, please follow the
instructions below:
1. Clean the shelf surface with ethanol.
2. Remove the protective paper from the
adhesive tape
3. Install the holder as described.
(Make sure the holder is pressed against the
edge of the shelf.)
4. Place the pipette onto the holder.
NOTE: An ergonomic, convenient carousel stand
for 6 Proline pipettes is also available (Cat.
No.725600).

5
5. Pipette Materials
Proline Single Channel
Push button Push button
rod (ABS) (Operating button)
(PA)
Grip Cover
(PA) Tip ejector
(PA)
Handle
(ASA/PC)
Display cover
(PC)
Tip Ejector
collar (PA)
Tip cone
2.5–1000 µl (PVDF)
2000–5000 µl (PPS)
Push button Push button rod
(ABS) (PA)
Grip cover
(PA) Tip Ejector
(PA)
Handle
(ASA/PC)
Display cover
(PC)
Tip cone
housing
(ASA/PC)
Tip cone,
soft sealing
Tip ejector (PVDF)
bar (PETP) (TPU)
(PVDF)
Hard tip
cone
(PC)
Proline Multichannel

6
6. Operating the pipette
6.1. Volume setting
The volume of the pipette is clearly shown through
the handle display window. The delivery volume
(variable volume pipettes only) is set by turning
the operating button clockwise or anticlockwise.
When setting the volume, please make sure that:
1. The desired delivery volume clicks into place.
2. The digits are completely visible in the display
window.
3. The selected volume is within the pipette’s
specified range.
Do not turn the operating button outside the
volume range because it may jam the mechanism
and damage the pipette.
6.2. Sealing and ejecting tips
Sartorius tips are recommended for use with
Proline pipettes. Before fitting a tip ensure that
the pipette tip cone is clean. Press the tip on the
cone of the pipette firmly to ensure an airtight
seal. The seal is tight when a visible sealing ring
forms between the tip and the black tip cone.
Each pipette is fitted with a tip ejector to help
eliminate the safety hazards associated with
contamination. The tip ejector needs to be pressed
firmly downwards to ensure proper tip ejection.
Make sure that the tip is disposed of into a
suitable waste container.
6.3. Two-in-one pipettes
The Proline fixed volume pipettes 5 and 10 µl, and
variable volume pipettes 0.5-10 µl can be used
with both 10 µl and 200 µl tips. The pipettes are
supplied with two ejector collars and are factory
fitted with the ejector suitable for 10 µl tips.
When using 200 µl tips, please change the ejector
collar by following the instructions below:
1. Push the tip ejector down (1.).
2. Push the opening tool pin between the
ejector bar and ejector collar to release the
locking mechanism (2.).
3. Pull the ejector collar off (3.).
4. Install the ejector collar by pushing the collar
locking pin into the ejector bar hole, keeping
the tip ejector pushed down.
1.
2.
3.

7
5. Release the tip ejector and test that it runs
smoothly.
6.4. Protective filters
The new tip cones of Sartorius pipettes (>10
µl) allow the use of a exchangeable filter as an
option. The filter prevents liquids and aerosols
from entering the pipette. Two types of filters are
available: a Safe-Cone Filter Standard and a Safe-
Cone Filter Plus (see cat.no. in Chapter 2.1). Plus
filters block the passage of liquids completely. The
filters do not affect the calibration of the pipette.
NOTE: Change the filter on the tip cone regularly
(after 50-250 pipettings).
7. Pipetting techniques
1. Make sure that the pipette, tips and the liquid
are at the same temperature.
2. Make sure that the tip is firmly attached to
the tip cone.
3. Hold the pipette vertically when aspirating
the liquid and place the tip only a few
millimetres into the liquid.
4. Prerinse the tip before aspirating the liquid
by filling and emptying the tip 5 times. This is
important especially when dispensing liquids
which have a viscosity and density different
from water.
5. Always control the operating button
movements with the thumb to ensure
consistency.
7.1. Forward pipetting
This technique employs the blow-out function
ensuring complete delivery of the liquid.
1. Depress the operating button to the first stop.
2. Place the tip(s) just under the surface of the
liquid (2-3 mm) and smoothly release the
operating button up to the starting position.
Carefully withdraw the tip from the liquid,
touching against the edge of the container
to remove excess.
3. Liquid is dispensed by gently depressing the
operating button to the first stop. After a
short delay continue to depress the operating
button to the second stop (= blow-out). This Starting position

8
procedure will empty the tip(s) and ensure
accurate delivery.
4. Release the operating button to the starting
position. If necessary change the tip(s) and
continue pipetting.
7.2. Reverse pipetting
A selected volume plus an excess is aspirated
into the tip. The delivery is done without blow-
out, and so the excess volume remains in the tip.
The reverse technique is recommended for high
viscosity, biological or foaming liquids, or very
small volumes of liquid.
1. Depress the operating button all the way to
the second stop. Place the tip(s) just under the
surface of the liquid (2-3 mm) and smoothly
release the operating button up to the
starting position.
2. Withdraw the tip(s) from the liquid touching
against the edge of the container to remove
excess.
3. Deliver the preset volume by smoothly
depressing the operating button to the first
stop. Hold the operating button at the first
stop. The liquid that remains in the tip(s)
should not be included in the delivery.
4. The remaining liquid should now be discarded
with the tip(s) or delivered back into the
container vessel.
NOTE: If reverse pipetting technique is used the
pipette might need recalibration.
8. Storage
When not in use it is recommended that your
Proline pipette is stored in a vertical position. See
Installing the pipette holder (Chapter 4).
9. Testing the
performance and
recalibration
It is recommended to check the performance of
your pipettes regularly (e.g. every 3 months) and
always after in-house maintenance. However,
the user should establish a regular testing
routine for their pipettes with regard to accuracy
First stop
Second stop