Sartorius Vivaspin Filtrate User manual

Instructions for Use
Vivaspin®Filtrate
Centrifugal Ultrafiltration Devices for General Laboratory Use
3104669-001-00

Contents
Instructions for Use Vivaspin®Filtrate 3
Contents
1 About these Instructions.................................................5
1.1 Scope................................................................5
1.2 Target Groups .......................................................5
1.3 Symbols Used........................................................6
1.3.1 Warnings in Operation Descriptions .........................6
1.3.2 Other Symbols ...............................................6
2 Safety Instructions .......................................................7
2.1 General Functions ...................................................7
2.2 Personnel Qualification..............................................8
2.3 Significance of these Instructions....................................8
2.4 Functionality of the Product .........................................8
3 Product Description .....................................................9
3.1 Product Overview ...................................................9
3.2 Product Symbols....................................................10
4 Process Preparation......................................................11
4.1 Scope of Delivery ....................................................11
4.2 Unpacking ...........................................................11
5 Operation...............................................................12
5.1 Pre-Rinsing the Product ............................................12
5.2 Sanitizing the Product ..............................................13
5.3 Performing Filtration................................................13
5.3.1 Applying the Sample ........................................13
5.3.2 Performing Filtration ........................................14
5.3.3 Removing the Sample.......................................15
6 Storage .................................................................16
6.1 Storing the Product.................................................16

Contents
4 Instructions for Use Vivaspin®Filtrate
7 Disposal.................................................................17
7.1 Decontaminating the Product ......................................17
7.2 Disposing of the Product ...........................................17
8 Technical Specifications ................................................18
8.1 Dimensions .........................................................18
8.2 Materials............................................................18
8.3 Ambient Conditions ................................................19
8.4 Equipment Required ...............................................19
8.4.1 Centrifuges..................................................19
8.4.2 Pipettes .....................................................19
8.5 Operating Conditions .............................................20
8.6 Sanitizing Methods ................................................20
8.7 Typical Performance Characteristics................................21

Instructions for Use Vivaspin®Filtrate 5
About these Instructions
1 About these Instructions
1.1 Scope
These instructions are part of the product. These instructions apply to the fol-
lowing versions of the product:
Vivaspin®Filtrate Quantity Prod. no.
5 kDa CTA 12 13229----------E
10 kDa CTA 12 13239----------E
20 kDa CTA 12 13249----------E
100 kDa PES 12 13269---------GE
300 kDa PES 12 13279----------E
1.2 Target Groups
The instructions are addressed to the following target groups. The target
groups must possess the knowledge specified below.
Target Group Knowledge and Qualifications
Operator The operator is familiarwith the device and the associated
work processes. The operator understands the hazards
which may arise when working with the device, and knows
how to prevent them.

6 Instructions for Use Vivaspin®Filtrate
About these Instructions
1.3 Symbols Used
1.3.1 Warnings in Operation Descriptions
NOTICE
Denotes a hazard that may result in property damage if it is not avoided.
1.3.2 Other Symbols
t  Required action: Describes actions that must be carried out.
The actions in the sequence must be carried out in succession.
y Result: Describes the result of the actions carried out.

Instructions for Use Vivaspin®Filtrate 7
Safety Instructions
2 Safety Instructions
2.1 General Functions
The product is intended for the ultrafiltration of biological and aqueous
solutions with small sample volumes, e.g. for protein removal.
The filtration process must be carried out in a centrifuge. For this purpose, the
sample solution must be put into the product and the product must be in-
serted into a centrifuge. Due to the centrifugal forces, the particles or macro-
molecules that are sufficiently larger than the nominal pore size of the mem-
brane are removed from the sample solution.
The product is supplied non-sterile. It is intended for single use and must be
disposed of after one use.
The product is intended exclusively for use in accordance with these
instructions. Any further use beyond this is considered improper.
Operating Conditions for the Product
The product is intended for general laboratory use.
The product may only be used with the equipment and under the operating
conditions described in the Technical Data section of these instructions.

8 Instructions for Use Vivaspin®Filtrate
Safety Instructions
2.2 Personnel Qualification
Persons without sufficient knowledge in the safe use of the device can injure
themselves and others.
If a specific qualification is required for an activity:The target group is indicat-
ed. If no qualification is specified: The activity can be carried out by the target
group "operator".
2.3 Significance of these Instructions
Failure to follow the instructions might have serious consequences, e.g. dan-
ger to individuals.
t Read the instructions carefully and completely. The instructions for action
build on each other.
t Ensure that the information contained in these instructions is available to
all individuals working with the product.
2.4 Functionality of the Product
A damaged product orworn parts can lead to malfunctions or cause hazards
which are difficult to identify.
t Only operate the product when it is safe and in perfect working order.

Instructions for Use Vivaspin®Filtrate 9
Product Description
3 Product Description
3.1 Product Overview
Fig.1: Product overview (example)
Pos. Description
1 Dust cap
2 Centrifuge tube
3Filtrate vessel with
membrane
1
2
3

10 Instructions for Use Vivaspin®Filtrate
Product Description
3.2 Product Symbols
Symbol Description
Qty Quantity
Catalogue number
Batch number
Use by
Non-sterile product
Do not re-use
Consult instructions for use
Temperature limits