Sartorius Vivaspin Filtrate User manual

Instructions for Use
Vivaspin®Filtrate
Centrifugal Ultrafiltration Devices for General Laboratory Use
3104669-001-00

Contents
Instructions for Use Vivaspin®Filtrate 3
Contents
1 About these Instructions.................................................5
1.1 Scope................................................................5
1.2 Target Groups .......................................................5
1.3 Symbols Used........................................................6
1.3.1 Warnings in Operation Descriptions .........................6
1.3.2 Other Symbols ...............................................6
2 Safety Instructions .......................................................7
2.1 General Functions ...................................................7
2.2 Personnel Qualification..............................................8
2.3 Significance of these Instructions....................................8
2.4 Functionality of the Product .........................................8
3 Product Description .....................................................9
3.1 Product Overview ...................................................9
3.2 Product Symbols....................................................10
4 Process Preparation......................................................11
4.1 Scope of Delivery ....................................................11
4.2 Unpacking ...........................................................11
5 Operation...............................................................12
5.1 Pre-Rinsing the Product ............................................12
5.2 Sanitizing the Product ..............................................13
5.3 Performing Filtration................................................13
5.3.1 Applying the Sample ........................................13
5.3.2 Performing Filtration ........................................14
5.3.3 Removing the Sample.......................................15
6 Storage .................................................................16
6.1 Storing the Product.................................................16

Contents
4 Instructions for Use Vivaspin®Filtrate
7 Disposal.................................................................17
7.1 Decontaminating the Product ......................................17
7.2 Disposing of the Product ...........................................17
8 Technical Specifications ................................................18
8.1 Dimensions .........................................................18
8.2 Materials............................................................18
8.3 Ambient Conditions ................................................19
8.4 Equipment Required ...............................................19
8.4.1 Centrifuges..................................................19
8.4.2 Pipettes .....................................................19
8.5 Operating Conditions .............................................20
8.6 Sanitizing Methods ................................................20
8.7 Typical Performance Characteristics................................21

Instructions for Use Vivaspin®Filtrate 5
About these Instructions
1 About these Instructions
1.1 Scope
These instructions are part of the product. These instructions apply to the fol-
lowing versions of the product:
Vivaspin®Filtrate Quantity Prod. no.
5 kDa CTA 12 13229----------E
10 kDa CTA 12 13239----------E
20 kDa CTA 12 13249----------E
100 kDa PES 12 13269---------GE
300 kDa PES 12 13279----------E
1.2 Target Groups
The instructions are addressed to the following target groups. The target
groups must possess the knowledge specified below.
Target Group Knowledge and Qualifications
Operator The operator is familiarwith the device and the associated
work processes. The operator understands the hazards
which may arise when working with the device, and knows
how to prevent them.

6 Instructions for Use Vivaspin®Filtrate
About these Instructions
1.3 Symbols Used
1.3.1 Warnings in Operation Descriptions
NOTICE
Denotes a hazard that may result in property damage if it is not avoided.
1.3.2 Other Symbols
t  Required action: Describes actions that must be carried out.
The actions in the sequence must be carried out in succession.
y Result: Describes the result of the actions carried out.

Instructions for Use Vivaspin®Filtrate 7
Safety Instructions
2 Safety Instructions
2.1 General Functions
The product is intended for the ultrafiltration of biological and aqueous
solutions with small sample volumes, e.g. for protein removal.
The filtration process must be carried out in a centrifuge. For this purpose, the
sample solution must be put into the product and the product must be in-
serted into a centrifuge. Due to the centrifugal forces, the particles or macro-
molecules that are sufficiently larger than the nominal pore size of the mem-
brane are removed from the sample solution.
The product is supplied non-sterile. It is intended for single use and must be
disposed of after one use.
The product is intended exclusively for use in accordance with these
instructions. Any further use beyond this is considered improper.
Operating Conditions for the Product
The product is intended for general laboratory use.
The product may only be used with the equipment and under the operating
conditions described in the Technical Data section of these instructions.

8 Instructions for Use Vivaspin®Filtrate
Safety Instructions
2.2 Personnel Qualification
Persons without sufficient knowledge in the safe use of the device can injure
themselves and others.
If a specific qualification is required for an activity:The target group is indicat-
ed. If no qualification is specified: The activity can be carried out by the target
group "operator".
2.3 Significance of these Instructions
Failure to follow the instructions might have serious consequences, e.g. dan-
ger to individuals.
t Read the instructions carefully and completely. The instructions for action
build on each other.
t Ensure that the information contained in these instructions is available to
all individuals working with the product.
2.4 Functionality of the Product
A damaged product orworn parts can lead to malfunctions or cause hazards
which are difficult to identify.
t Only operate the product when it is safe and in perfect working order.

Instructions for Use Vivaspin®Filtrate 9
Product Description
3 Product Description
3.1 Product Overview
Fig.1: Product overview (example)
Pos. Description
1 Dust cap
2 Centrifuge tube
3Filtrate vessel with
membrane
1
2
3

10 Instructions for Use Vivaspin®Filtrate
Product Description
3.2 Product Symbols
Symbol Description
Qty Quantity
Catalogue number
Batch number
Use by
Non-sterile product
Do not re-use
Consult instructions for use
Temperature limits

Instructions for Use Vivaspin®Filtrate 11
Process Preparation
4 Process Preparation
4.1 Scope of Delivery
Item Quantity
Product, packed in a cardboard box, according to
packaging unit
12
Instructions for Use 1
4.2 Unpacking
Procedure
t NOTICE Risk of product malfunctions due to exceeding the usability!
Check the usability of the product (see specification on packaging). Dis-
pose of products forwhich the usability has been exceeded.
t Unpack the product.

12 Instructions for Use Vivaspin®Filtrate
Operation
5 Operation
5.1 Pre-Rinsing the Product
The membrane in the product may contain traces of glycerin. If this substance
can interfere with the analysis of the sample: Rinse the membrane before
filtration.
Procedure
t Remove the dust cap.
t Slide out the filtrate vessel and place it with the open end downwards. Do
not touch the membrane.
t Dip the filtrate vessel with the membrane facing downwards in buffer solu-
tion or deionized water for 5 minutes.
t Alternatively, use a pipette to apply a filling volume of buffer solution or
deionized water into the centrifuge tube.
t Slide the filtrate vessel into the centrifuge tube with the membrane facing
downwards.
t Wash the buffer solution or deionized water through the membrane with
the help of a suitable centrifuge.
t Decant the buffer solution or deionized water from the centrifuge tube
and filtrate vessel.
t If the pre-rinsed product is not used immediately: Cover the surface of the
membrane with buffer solution or water, replace the dust cap to avoid
evaporation and store the product in the refrigerator. The membrane must
not dry out.

Instructions for Use Vivaspin®Filtrate 13
Operation
5.2 Sanitizing the Product
The product can be sanitized before use. The sanitizing method must be suit-
able for the product (see Chapter “8.6 Sanitizing Methods“, page 20).
Procedure
t Remove the dust cap.
t Sanitize the product using the desired sanitizing method.
t Empty the product.
5.3 Performing Filtration
5.3.1 Applying the Sample
It is recommended that a pipette is used to apply the sample into the prod-
uct. The pipette must be compatible with the product (see Chapter “8.4.2 Pi-
pettes“, page 19).
Please ensure that the molecular weight cut-off (MWCO) of the product is
suitable for the size of the target molecule(s) to be removed or concentrated.
In order to ensure maximum removal or recovery of the target molecule, it is
recommended to select a MWCO that is at least 50% below the size of the
target molecule.
NOTICE
Risk of product malfunctions due to using unsuitable samples!
t Do not apply samples containing organic solvents into the product.
NOTICE
Risk of product malfunctions or damage to the centrifuge due to exceeding
the maximum filling volume!
t Do not exceed the maximum filling volume (see Chapter “8.5 Operating
Conditions“, page 20).

14 Instructions for Use Vivaspin®Filtrate
Operation
Procedure
t Check whether the MWCO of the product is suitable for the application.
t Remove and discard the dust cap.
t Slide out the filtrate vessel and place it with the open end downwards. Do
not touch the membrane.
t Apply the sample into the centrifuge tube using a pipette. Comply with
the maximum filling volume. For non-coagulated blood samples, pre-cen-
trifuge the sample for 2 minutes without the filtrate vessel.
t Slide the filtrate vessel into the centrifuge tube with the membrane facing
downwards and leave for 5 minutes so that the membrane is wetted by the
sample.
5.3.2 Performing Filtration
Procedure
t Insert the product into the centrifuge. For samples containing lipids (e.g.
milk), only use a fixed angle rotor.
t NOTICE Risk of product malfunctions or damage to the centrifuge. Com-
ply with the approved centrifugation limit values (see Chapter “8.5 Oper-
ating Conditions“, page 20).
t Centrifuge the product in the centrifuge until the desired filtrate volume
or concentration level is achieved. For concentrated protein samples (e.g.
blood, serum), start the filtration at half the maximum RCF for 5 minutes.

Instructions for Use Vivaspin®Filtrate 15
Operation
5.3.3 Removing the Sample
Procedure
t If the filtration or concentration is complete: Remove the product from the
centrifuge.
t NOTICE Risk of reduced sample volume due to diffusion. Remove the
sample from the filtrate vessel with a pipette as soon as possible after
performing filtration.
t If the concentrate is required: Remove the filtrate vessel from the
centrifuge tube using opened forceps. Use a pipette to remove the
concentrate from the centrifuge tube.
t If the membrane was pre-rinsed before filtration: Decant the filtrate and
concentrate.

16 Instructions for Use Vivaspin®Filtrate
Storage
6 Storage
6.1 Storing the Product
If the product has been unpacked and the membrane has been pre-rinsed:
The membrane must be protected against drying out. For this purpose, the
membrane must be stored in a moist and cool condition.
NOTICE
Risk of damage to the product due to improper storage!
t Complywith the storage specifications.
Procedure
t If the product is packaged: Store the product in the packaging.
t If the product has been unpacked and membrane has been pre-rinsed:
t Remove the dust cap.
t Cover the membrane with buffer solution or water.
t Replace the dust cap.
t Store the product according to the ambient conditions (see Chapter “8.3
Ambient Conditions“, page 19).

Instructions for Use Vivaspin®Filtrate 17
Disposal
7 Disposal
7.1 Decontaminating the Product
If the product has come into contact with hazardous substances: Steps must
be taken to ensure proper decontamination and declaration. The operator of
the product is responsible for adhering to local government regulations on
the proper decontamination and declaration for transport and disposal.
Procedure
t If the product has come into contact with hazardous substances:
Decontaminate the product.
7.2 Disposing of the Product
The product must be disposed of properly. The packaging is made of environ-
mentally friendly materials that can be used as secondary raw materials.
Requirements
The product must be decontaminated.
Procedure
t Dispose of the product in accordance with local government regulations.
t Dispose of the packaging in accordance with local government regula-
tions.

18 Instructions for Use Vivaspin®Filtrate
Technical Specifications
8 Technical Specifications
8.1 Dimensions
Unit Value
Dimensions
Length × Diameter mm 93 × 14
Active membrane surface cm20.79
Weight g 10
8.2 Materials
Materials
Filtrate vessel Styrene Acrylonitrile (SAN)
Centrifuge tube Polystyrene (PS)
Dust cap Polyethylene (PE)
Membrane Cellulose Triacetate (CTA) or
Polyethersulfone (PES)

Instructions for Use Vivaspin®Filtrate 19
Technical Specifications
8.3 Ambient Conditions
Unit Value
Storage temperature
When packed °C +15 – +30
When unpacked, with membrane kept
moist
°C +2 – +8
8.4 Equipment Required
8.4.1 Centrifuges
Unit Value
Swing bucket rotor or fixed angle rotor
Minimum rotor angle for fixed angle rotor 25°
Rotoraccepting centrifuge tubes with the fol-
lowing features
Volume mL 15
Diameter mm 17
Base Conical or
flat
8.4.2 Pipettes
Pasteur pipette, variable volume or fixed volume pipette for sample appli-
cation and concentrate or filtrate retrieval.

20 Instructions for Use Vivaspin®Filtrate
Technical Specifications
8.5 Operating Conditions
Unit Value
Filtration Volumes
Filling volume, minimum mL 0.5
Filling volume, maximum mL 2.5
Membrane hold-up volume, minimum µL <5
Dead stop volume1µL 100
Relative Centrifugal Force, maximum
Centrifuge with swing bucket rotor g2,500
Centrifuge with fixed angle rotor g2,000
1 The dead stop volume mayvary depending on the type and concentration
of the sample, operating temperature and | or centrifuge rotor
8.6 Sanitizing Methods
Rinsing with 70% ethanol or with sanitizing gas mixture, e.g. ethylene oxide
Not suitable for autoclaving