Sigma 4-15C User manual

Centrifuge
4-15C
Operating Manual

Operating Manual SIGMA 4-15C, page 3 of 95
03/06
Dear customer,
Congratulations for purchasingaSIGMAlaboratorycentrifuge.Youhaveselected adevice
which combines manyadvantages.
Awide spectrum ofprogrammingoptions and an electronic operation control allowatrouble-
free use ofthe centrifuge. With its 3-phase drive, maintenance-free, quiet operation without
anycarbon dust pollutionis guaranteed.
Your device is equipped with user-friendlyoptions which makethe operation and standard
settingseasier for you. Built-in error-detectingfunctions keep the user fromentering incorrect
values and checkthe complete operation.
Aspecial advantageisthe storagecapacitythe centrifugeoffers. Theinstrumenthas a
programmemorywhich can store upto50 data sets andis capable ofkeepingthe last run
programin its memoryfor an unlimited amount oftime allowingtheprogram tobe restarted
at anytime - even ifthe centrifugewas turned off in between. All important operation
parameterscanbe seenat aglance.
The settingsare executed via the knob in the control panel which hasacoated surface
protectingthe device against moisture and dust. In addition, the interior ofthe centrifugeis
also easyto clean. Weare able to offer you adevice that combines functional varietywith
practical applications.
Wethankyou for your confidence andwish you a successfulapplication of the centrifuge.
SIGMA LaborzentrifugenGmbH
P.O. Box1713 - 37507Osterode/Germany
Phone [phone] - Fax[phone]
Internet: www.sigma-zentrifugen.de
eMail: [e-mail]
SIGMA Service-Tel. [phone]

Operating Manual SIGMA 4-15C, page 5 of 95
03/06
Konformit tserkl rung
(73/23/EWG; 89/336/EWG;98/37/EWG)
Statement of Conformity
(73/23/CEE; 89/336/CEE; 98/37/CEE)
Déclaration de conformité
(73/23/CEE; 89/336/CEE; 98/37/CEE)
Die nachfolgendbezeichnete Maschine wurdein bereinstimmungmit denRichtlinien
73/23/EWG; 89/336/EWG und98/37/EWG hergestellt undgeprüft.
The followingmachine ismanufactured and testedin compliance with directions 73/23/CEE;
89/336/CEE and98/37/CEE.
La machine désignéeci-dessous est produit et examinéconforme auxdirectives 73/23/CEE;
89/336/CEE et 98/37/CEE
Bezeichnungder Maschine: Laborzentrifuge
Machine: LaboratoryCentrifuge
Désignation dela machine: Centrifugeusede laboratoire
Maschinentyp : 4 - 15
Type:
Type de la machine:
Bestell Nr. : 10750, 10751, 10752, 10753, 10730, 10731, 10732
Part No.: 10733
Réf. usine:
Normen: EN 61010-2-020
Standards: EN 61000-3-2 ; EN 61000-3-3
Normes: EN 61326
Sigma Laborzentrifugen
An der UnterenSöse 50
D-37520 Osterode
01.02.2002Geschäftsführer
ManagingDirector
Directeur Gérant
........................................................................
Fabr. Nr. Serial No. Num ro de fabrique
Konformitätserklärung dreisprachig 4-15 20020201.DOC

Operating Manual SIGMA 4-15C, page 7 of 95
03/06
General Information
1.1 Technical data
1.2 Suitable accessories
1.3 Scope of supply
1.4 Standardsand regulations
1.5 Safetyinstructions
1.6 Symbol table
Descriptionof theCentrifuge
2.1 General outlay
2.2 Construction and constructive safetymeasures
2.3 Drive
2.4 Operation anddisplay
2.5 Electronic control
2.6 Safetydevices
2.6.1 Lid lock, cover closing device
2.6.2 Imbalancemonitoringsystem
2.6.3 Rotor monitoring
2.6.4 Standstill monitoring
2.6.5 System check
2.6.6 Groundwire check
InstallationandStart-up
3.1 Unpackingof thecentrifuge
3.1.1 Transport safetydevice
3.2 Installation
3.2.1 Site
3.2.2 Connection/Fuse
3.2.3 Fuses / emergencycircuit breaker on site
3.3 Installation of rotorsand accessories
3.3.1 Fastening of angle rotorswith hermeticallysealedlid
3.4 Initial start-up
3.4.1 Switchingonof thecentrifuge
3.4.2 Opening lid
3.4.3 Installation of arotor

Operating Manual SIGMA 4-15C, page 8 of 95
03/06
Operating Elements
4.1 Operating panel
4.1.1 Start-key
4.1.2 Stop-key
4.1.3 Lid-key
4.1.4 Knob
4.2 Display
4.2.1 Set
4.2.2 Speed
4.2.3 Relative CentrifugalForce (RCF)
4.2.4 Time
4.2.5 Program
4.2.6 Rotor
4.2.7 Parameters
4.2.7.1 Acceleration
4.2.7.2 Deceleration
4.2.7.3 Radius
4.2.7.4 Density
4.2.7.5 Start delay
4.2.7.6 Automatic lid opening after endof run
4.2.8 Configuration
Operation
5.1 Selection, displayand alteration of program parameters
5.1.1 Selection and alteration ofthe parameters and activation ofthe start
delayand the automatic lid openingafter end of run
5.1.2 Selection and alteration ofthe rotor part number
5.1.3 Alteration of the parameter values duringthe centrifuge run
5.1.4 Alteration of the configuration
5.1.4.1 Curves
5.1.4.2 Creation of curves for variable accelerationsand decelerations
5.1.4.3 Alteration of existing curves
5.1.5 Alteration of the contrast
5.1.6 Imbalancemonitoring
5.1.7 Shortrunand faststop
Programming
6.1 Load, save anddelete programs
6.1.1 Load a program
6.1.2 Save a program
6.1.3 Delete a program

Operating Manual SIGMA 4-15C, page 9 of 95
03/06
Notes for Centrifugation
7.1 Practical notes for centrifugation
7.2 Forbidden centrifugingoperations
Care andMaintenance
8.1 Care and cleaningof centrifuge
8.2 Care and cleaningof accessories
8.3 Rotor pins
8.4 Glass breakage
8.5 not applicable
8.6 Sterilization and disinfection ofrotor chamber and accessories
8.6.1 Autoclaving
8.7 Checksbyoperator
Appendix
9.1 Slope ofthe specified curves, linear curves
9.2 Quadratic curves
9.3 Entrylimitations
9.4 Mathematical relations
9.4.1 Relative CentrifugalForce (RCF)
9.4.2 Density
9.5 Error correction
9.5.1 Centrifugecannot be started
9.5.2 Centrifugedecelerates during operation
9.5.3 Lid cannot be opened/closed
9.5.4 Emergencylid release
9.5.5 Problems with thecentrifuge?
9.6 Error codes
9.7 Speed-RCF-diagram
9.8 Declaration of decontamination/Return declaration
9.9 Form program data
9.10 Leaflet

Operating Manual SIGMA 4-15C, page 11 of 95
03/06
1.1 Technical Data
Manufactuer: S I G MA
LaborzentrifugenGmbH
D-37520 Osterode
Germany
Type: 4-15C
Electr. connection:
Protection class: see nameplate I
Power consumption (kVA):
Ratedpower (kW):
Max. current (A):
1,5
1,2
6,5 (230V/50Hz) respectively
13,0 (120 V/60 Hz)
Power data:
Max. speed (rpm):
Max. capacity(l):
Max. gravitational field (x g):
Max. kin. energy(Nm):
13 500
2
20 376
50 487
Further parameters
Time range:
Programs:
Acceleration curves:
Deceleration curves:
Radius:
Rotor part no.:
9 h, 59 min/continuous operation
50 No. 1 - 50
10 linear No. 0 - 9
10 quadratic No.10 - 19
10 freelyprogrammable
No. 20 - 29
9 linear No. 1 - 9
1 brakeless No. 0
10 quadratic No.10 - 19
10 freelyprogrammable
No. 20 –29
max./min. s. 1.2
s. chapter 1.2
Dimensions:
Depth(mm):
Width (mm):
Height (mm):
Weight (kg):
EMC (acc. to EN 55011):
Noise level (dBA):
685
490
405
82 kg
Class B
75 (at max. speed)
Notes ofuser:
Serial number:
Supplydate:
Inventorynumber:
Location:
Responsibility:
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.....................................................
The figures are validfor anambient temperature of23 °C+/- 2 °Candnominalvoltage +/- 5%.
(Allowableambient temperature +4 °C - +40 °C; max. humidity80 %.)
Subject to technical alterations.

Operating Manual SIGMA 4-15C, page 13 of 95
03/06
1.2 Accessories Suitablefor SIGMA4-15C
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
11150 Swing-out rotor for 4 buckets 13215, 13220,
13221, 13231, 13234, 13235, 13236, 13350
13220 Bucket , aluminium, for microtitre plates,
max. alloweddata with rotor 11150:
radius edge16.6 cm,
radius max. 16.0 cm,
radius min. 11.3cm,
max. plate height 54 mm
4 100 3 120
3 007
2 124
13221 Bucket , aluminium, for microtitre plates,
max. alloweddata with rotor 11150:
radius edge16.6 cm,
radius max. 16.0 cm,
radius min. 10.0cm,
max. plate height 56 mm, incl. plate holder
17979
4 500 3 758
3 622
2 264
13231 Bucket, aluminium, for 1 pointed bottom
bottle150or 200 ml e.g. 15175, approx.
61.5 x140 mm, incl. adapter 13175,
suitable for 11150, max. radius 19.0cm,
min. radius 8.7cm, 4 500 4 302
13175 Adapter for pointed bottom bottle15175,
suitable for 13231, 17347
13233 Bucket, aluminium, for 50 RIA-tubes 12.5
x 70 - 90mm, e.g. 15060, suitable for 11150
13234 Bucket, aluminium, for 4 tubes100 ml,
45x 100mm, e.g. 15100, 15102, 15103,
15106, incl. rubber cushion 16051, suitable
for 11150, max. radius 17.8 cm, min. radius
7.8 cm 4 300 3 680
13235 Bucket, aluminium, for 7 culture tubes
50 ml 15151, suitable for 11150, max. radius
19.0 cm, min. radius 9.3 cm 4 500 4 302

Operating Manual SIGMA 4-15C, page 14 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
13236 Bucket, aluminium, for 12 culture tubes
15 ml 15115, suitable for 11150, max. radius
18.8 cm, min. radius 9.6 cm 4 500 4 246
13350 Round bucket, aluminium, withthread, incl.
O-ring482840, for the 500 ml bottles 15500,
15501 and for theround carriers ∅85 mm
17350 up to 17402, sealable with caps
17134, 17135, suitable for 11150, max.
radius 19.0cm, min. radius 8.7 cm
4 500 4 302
17134 Aluminium sealingcapfor 13350
17135 Polysulfone sealingcap, clear, for 13350
17350 Round carrier for 24 reactionvials 0.5-
0.75 ml, ∅7.9/10x 28/31 mm, e.g. 15005,
polypropylene
17351 Round carrier for 12 Monovettes, max.
∅15.5/18x 50 - 75 mm, polypropylene
17352 Round carrier for 25 RIA-tubes 5 ml, max.
∅12.5 x65 - 80mm, e.g. 15060,
polypropylene
17353 Round carrier for 16 reactionvials 1.5-
2.2 ml, max. ∅11 mm, e.g. 15008, 15040,
polypropylene
17354 Round carrier for 16 glass tubes7 ml, max.
∅12.5 x85 - 115mm, e.g. 15007, 15027,
polypropylene
17355 Round carrier for 12 tubes withscrewcap
10-12 ml, max. ∅16.2/19 x 65x 90mm, e.g.
13026 plus17126, 15000, 15010, 15039,
polypropylene
17356 Round carrier for 16 Vacutainer/hemolyse/
RIA-tubes 5-6 ml, max. ∅13.5/17.5 x70 -
90 mm, polypropylene

Operating Manual SIGMA 4-15C, page 15 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
17358 Round carrier for 12 tubes 10-15ml, max.
∅17.2/19.5 x90 - 115mm, e.g. 15015,
15020, 15022, 15023, 15024 and Monovettes
9 ml and10 ml, polypropylene
17359 Round carrier for 9 culture tubes15 ml,
max. ∅cap 23mm, e.g. 15115, Greiner or
Corning tubes, polypropylene
17360 Round carrier for 10 culturetubes15 ml,
max. ∅cap 22mm, e.g. 15115, Greiner or
Corning tubes, polypropylene
17362 Round carrier for 5 sterilin tubes 30ml,
graduated upto20 ml, with skirt, incl. cap,
max. ∅25/31x 65 - 95 mm, polypropylene,
see www.bibby-sterilin.co.uk, no. 03008
17370 Round carrier for 5 tubes 25-30ml, max.
∅25.4/29x 85 - 115 mm, e.g. 15025, 15026,
15029, 15030, 15032, 15033, polypropylene
17375 Round carrier for 3 tubes 50 ml, max.
∅35/38 x90 - 110mm, e.g. 15049, 15050,
15056, polypropylene
17376 Round carrier for 4 tubes withscrewcap
40-50 ml, max. ∅29/34x 85 - 110mm, e.g.
13055 plus17054, 15051, 15052, 15054,
polypropylene
17377 Round carrier for 4 culture tubes50 ml
15151, polypropylene
17378 Round carrier for 4 culture tubes50 ml with
skirt, polypropylene
17385 Round carrier for 1 tube withscrewcap
78-85 ml, max. ∅38/40x 85 - 115mm, e.g.
13085 plus17185, 15074, 15075, 15076,
15080, polypropylene

Operating Manual SIGMA 4-15C, page 16 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
17390 Round carrier incl. rubber cushion16051
for 1tube100 ml, max. ∅45/50 x85 -
110 mm, e.g. 15100, 15102, 15103, 15106,
polypropylene
17395 Round carrier for 1 bottlewithscrewcap
125 ml, max. ∅51x 90 - 115mm, e.g. 15125,
polypropylene
17400 Round carrier for 1 bottlewithscrewcap
200 ml, max. ∅57x 90 - 115mm, e.g. 15202,
15203, polypropylene
17401 Round carrier incl. rubber cushion16250
for 1roundbottom tube 175-250ml, max.
∅57.5 x90 - 137mm, e.g. 15201, 15206,
15250, 15251, 15254, polypropylene
17402 Round carrier for 1 tube withpointed
bottom 150-250 ml, max. 60x 120 -
140 mm, polypropylene
17347 Round carrier for 1 bottlewithscrewcap
250 ml, max. 61.5x 90 - 125 mm, e.g.
13255, 15247, 15248, 15249 and for 15175
with adapter 13175 (suitablefor bucket
13350 without screwcap 17135),
polypropylene
13215 Rectangular bucket, aluminium, sealable
with cap17112, for the system of
rectangular carriers, max. tube length
115 mm, suitablefor rotor 11150, max.
radius 18.2cm, min. radius 8.8 cm 4 500 4 120
11156 Swing-out rotor for 6 buckets for the
following accessories,
data with 13115: max. radius 18.2 cm, min.
radius 9.5cm
data with 13127: max. radius 19.1 cm, min.
radius 9.7cm
4 500
4 500
4 120
4 324
11140 Swing-out rotor for 4 buckets for the
following accessories 5 000 4 668/4416

Operating Manual SIGMA 4-15C, page 17 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
13115 Rectangular bucket, aluminium, suitable for
the system of rectangular carriers, max.
tube length110 mm 5 000 4 416
13127 Rectangular bucket, aluminium, incl.
polysulfone sealingcap 17112, suitablefor
the system of rectangular carriers, max.
tube length115 mm 5 000 4 668
18000 Rectangular carrier, undrilled, for tubes 85 -
110 mm, polyallomer
18002 Rectangular carrier for 20 reaction vials
1.5-2.2 ml, max. ∅11 mm, e.g. 15008, 15040,
polypropylene
18003 Upper part for 18002for 20 reaction vials
1.5-2.2 ml, max. ∅11 mm, e.g. 15008, 15040
(can beused together with 18002),
polypropylene
18005 Rectangular carrier for 20 RIA-tubes 5 ml,
max. ∅12.2x 60 - 75 mm, flat and round
bottom, e.g. 15060, polyallomer
18007 Rectangular carrier for 20 glasstubes7 ml,
max. ∅12.3x 80 - 105 mm, flat andround
bottom, e.g. 15007, 15027, polyallomer
18009 Rectangular carrier for 20 hemolyse tubes,
max. ∅12.8x 70 - 90 mm, polypropylene
18010 Rectangular carrier for 12 tubeswithscrew
cap 10-12ml, max. ∅16.8/17.5 x60 - 85
mm, e.g. 13026 plus 17126, 15000, 15010,
15039, polyallomer
18012 Rectangular carrier for 12 Vacutainer-tubes
∅13.5/18x 65 - 90 mm, polypropylene
18015 Rectangular carrier for 12 tubes10-15ml,
max. ∅17 x90 - 105mm, e.g. 15015, 15020,
15022, 15023, 15024, polyallomer

Operating Manual SIGMA 4-15C, page 18 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
18016 Rectangular carrier for 4 culture tubes
15 ml 15115, polypropylene
18017 Rectangular carrier for 10 tubes15 ml and
tubes with cap, max. ∅17.2/18x 80 -
112 mm, e.g. Monovettes 9 ml and 10ml,
polypropylene
18025 Rectangular carrier for 5 glasstubes25 ml,
max. ∅24 x85 - 105mm, e.g. 15025, 15026,
polyallomer
18022 Rectangular carrier for 4 sterilin tubes
30 ml, graduatedupto20 ml, withskirt,
incl. cap, max. ∅25/31x 65 - 95mm,
polypropylene, see www.bibby-sterilin.co.uk,
no. 03008
18030 Rectangular carrier for 5 tubeswithscrew
cap 27-30ml, max. ∅25.4/27.5 x80 -
110 mm, e.g. 15029, 15030, 15032,
polypropylene
18050 Rectangular carrier for 2 tubes50 ml, max.
∅35/38 x85 - 110mm, e.g. 15049, 15050,
15056, polyallomer
18051 Rectangular carrier for 2 tubeswithscrew
cap 40-50ml, max. ∅29/35 x80 - 110mm,
e.g. 13055 plus 17054, 15051, 15052, 15054,
polypropylene
18052 Rectangular carrier for 2 culture tubes
50 ml 15151, polypropylene
18053 Rectangular carrier for 2 culture tubes
50 ml with skirt, max. ∅29.5/38 x85 -
118 mm, polypropylene
18085 Rectangular carrier for 1 tubewithscrew
cap 78-85ml, max. ∅38/40 x85 - 112mm,
e.g. 13085 plus 17185, 15074, 15075, 15076,
15080, polyallomer

Operating Manual SIGMA 4-15C, page 19 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
18100 Rectangular carrier for 1 tube100 ml, max.
∅45.5/48x 85 - 110 mm, e.g. 15100, 15102,
15103, 15106, polyallomer
18105 Rectangular carrier for 20 RIA-tubes 5 ml,
max. ∅12.2x 60 - 75 mm, flat and round
bottom, e.g. 15060, decantable, polyallomer
18107 Rectangular carrier for 20 glasstubes7 ml,
max. ∅12.3x 80 - 105 mm, flat andround
bottom, e.g. 15007, 15027, decantable,
polyallomer
18115 Rectangular carrier for 12 tubes10-15ml,
max. ∅17 x90 - 105mm, e.g. 15015, 15020,
15022, 15023, 15024, decantable,
polyallomer
18125 Rectangular carrier for 1 bottlewithscrew
cap 125 ml, max. ∅51 x 90 - 115mm, e.g.
15125, polypropylene
18200 Rectangular carrier for 1 bottlewithscrew
cap 200 ml, max. ∅57 x 90 - 115mm, e.g.
15202, 15203, polypropylene
13201 Round bucket for 1 glass tube200 ml
15201, 15206, incl. rubber cushion16250,
sealablewithaluminium cap17121
11141 Swing-out rotor 48x 15ml complete,
consisting of rotor 11140, 4rectangular
buckets 13115, 4carriers 18015 and
48 polystyrene tubes 15020, max. radius
15.7 cm, min. radius 6.1 cm 5 000 4 416
11157 Swing-out rotor 72x 15ml complete,
consisting of rotor 11156, 6rectangular
buckets 13115, 6carriers 18015 and72
polystyrene tubes 15020, max. radius
18.2 cm, min. radius 9.5 cm 4 500 4 120

Operating Manual SIGMA 4-15C, page 20 of 95
03/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
11144 Swing-out rotor for microtiter plates, incl.
2 buckets13145 andplate holder 17977,
radius edge12.85 cm,
radius max. 10.7 cm
radius min. 6.3cm,
max. plate height 64 mm
5 000 3 592
2 991
1 761
11118 Swing-out rotor for microtiter plates, incl.
2 buckets13218 andplate holder 17978,
radius edge15.15 cm,
radius max. 13.8 cm
radius min. 5.8cm,
max. plate height 80 mm,
buckets sealablewithcap 17108
5 500 5 124
4 667
1 961
17108 Rectangular sealing cap, polysulfone, clear,
incl. 4clips 17118, for bucket 13218
17978 Plate holder for bucket 13218
12165 Angle rotor 6 x78-85ml for sealedtubes,
e.g. 13085 plus 17185, 15074, 15075, 15076,
15080, incl. hermeticaluminium lid, max.
radius 9.8cm, min. radius 2.7cm, angle 25° 12 500 17 119
12166 Angle rotor 8 x40-50ml for sealedtubes,
e.g. 13055 plus 17054, 15051, 15052, 15054,
incl. hermeticaluminium lid, max. radius
9.6 cm, min. radius 3.3 cm, angle25° 12 500 16 770
12172 Angle rotor 12 x27-30ml for sealedtubes,
e.g. 15029, 15030, 15032, incl. hermetic
aluminium lid, max. radius 11.0 cm, min.
radius 5.1cm, angle 30° 12 500 19 216
12168 Angle rotor 20 x10-12ml for sealedtubes,
e.g. 13026 plus 17126, 15000, 15010, 15039,
incl. hermeticaluminium lid, max. radius
9.8 cm, min. radius 5.8 cm, angle25° 12 500 17 119
12130 Angle rotor 30 x1.5-2.2ml for reaction
vials, e.g. 15008, 15040, incl. hermetic
aluminium lid, max. radius 10.0 cm, min.
radius 6.7cm, angle 45° 13 500 20 376