SP Scientific LYO-SEA User manual

SAMPLE EXTRACTOR ASSEMBLY OPERATOR’S MANUAL
L YO -SEA FOR L YO S TAR II

Rev 005, 05/11 i
© SP Scientific 2011
Copyright © 2011 SP Scientific. All marks herein are used under license.
All brand or product names mentioned may be trademarks or registered trademarks of their respective
companies.
Part Number 200-31389-999
Rev 005, 05/11
SP Scientific
815 State Route 208
3538 Main Street
Gardiner, NY 12525 USA Stone Ridge, NY 12484
USA
[phone]
[phone]
SP Scientific Service
[phone]
SP Scientific Service Fax
[phone]
Website
http://www.spscientific.com/
This Sample Extractor Assembly Operator’s Manual contains confidential and proprietary information of
SP Scientific and may be used only by a recipient designated by and for purposes specified by SP
Scientific.
Reproduction of, dissemination of, modifications to, or the creation of derivative works from this Sample
Extractor Assembly Operator’s Manual, by any means and in any form or manner, is expressly prohibited,
except with the prior written permission of SP Scientific. Permitted copies of this document must retain all
proprietary notices contained in the original.
The information in this document is subject to change without prior notice. Always confirm with SP
Scientific that you are using the most current version of this document. SP Scientific is free to modify any
of its products and services, in any manner and at any time, notwithstanding the information contained in
this document.
THE CONTENTS OF THIS DOCUMENT SHALL NOT CONSTITUTE ANY WARRANTY OF ANY KIND,
EITHER EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES
OF MERCHANTABILITY AND/OR FITNESS FOR A PARTICULAR PURPOSE OR GIVE RISE TO ANY
LIABILITY OF SP SCIENTIFIC, ITS AFFILIATES OR ITS SUPPLIERS.
The terms and conditions governing the use of this Sample Extractor Assembly Operator’s Manual shall
consist of those set forth in written agreements with SP Scientific.

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© SP Scientific 2011
Important Symbols
WARNING! INJURY OR EVEN
DEATH MAY RESULT IF A
RECOMMENDATION MARKED
WITH THIS SYMBOL IS NOT
HEEDED.
CRUSH HAZARD. KEEP HANDS
CLEAR WHEN OPERATING DOOR.
ELECTRIC SHOCK DANGER!
USE APPROPRIATE CAUTION
TO AVOID INJURY OR DEATH.
CORROSIVE CHEMICAL. WEAR
SUITABLE GLOVES, SAFETY
GLASSES, AND PROTECTIVE
CLOTHING.
BURN DANGER! POTENTIALLY
HOT SURFACE. USE
APPROPRIATE CAUTION.
PROPERTY CAUTION! TO PREVENT
DAMAGE TO CHAMBER EQUIP-
MENT AND/OR LOAD, ADHERE TO
PROCEDURES MARKED BY THIS
SYMBOL.
DO NOT STORE FLAMMABLE
MATERIALS IN CHAMBER.
PRACTICAL OPERATING TIP.
THESE RECOMMENDATIONS
STREAMLINE UNIT OPERATION
AND PREVENT COMMON
OPERATOR ERRORS.
WEAR SAFETY GLASSES.
EXPLOSIVE MATERIALS HAZARD!
KEEP OBJECTS AWAY FROM HEAT.
Safety Warnings
Always transport the unit with care. Sudden jolts or drops may damage the refrigeration system.
Always observe all warning labels.
Always turn off the unit and disconnect the line cord from the available power source prior to performing anyservice or maintenance procedures.
Always turn off the unit and disconnect the line cord from the available power source prior to moving the unit.
Always empty the reservoir / bath chamber prior to moving the unit.
Never operate equipment with damaged line cords.
Never operate the unit without cooling fluid in the reservoir / bath chamber.
Never remove warning labels.
Never operate damaged or leaking equipment.

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© SP Scientific 2011
Warranty Information
Lyo-SEA Sample Extractor Assemblies are warranted by SP Scientific to be free of defects in material and
workmanship when operated under normal conditions as specified in the instructions provided in this manual. Please
take this opportunity to locate the serial tag on your new Lyo-SEA and record the information below for future
reference. SP Scientific also recommends that you complete and return your unit’s warranty registration card.
Model Number
Serial Number
Part Number
Limited Warranty
SP Scientific (the “Company”) shall warrant each of its products against defects in material or workmanship for a period
of 12 months from the date of shipment (whichever comes first) provided that the product is used in a reasonable
manner under appropriate conditions and consistent with the applicable operating instructions.
The obligation of the Company shall be, at its option, to repair or replace, without charge any parts that prove to be
defective within the warranty period, if the purchaser notifies the Company promptly in writing of such defect. No
product shall be returned to the Company without prior approval of the Company.
This limited warranty shall cover the costs of parts and labor to repair or replace all defective product(s) at the Seller’s
factory. For all products installed by the Company and located within the Company service travel areas, this warranty
shall cover transportation charges to ship the product to and from the Company’s factory and/or the costs of travel,
room and board if the Company’s employees conduct repair at the Buyer’s location. In lieu of repair or replacement at
the Company’s factory, the Company may, in its discretion, authorize a third party to perform the repair or replacement
at the Buyer’s location, and at the Company’s sole expense.
The Company shall not be responsible for labor charges payable with respect to persons other than Company
employees. Replacement or repair of parts pursuant to this warranty shall not in any way extend the original warranty
period. The Company shall not be responsible for any unauthorized repairs, replacements or product modifications, nor
will it be responsible for any product failures resulting from such unauthorized repairs, replacements or product
modifications negligently or otherwise made by persons other than Company employees or authorized representatives
of the Company. The buyer shall assume transportation charges to ship the product to and from the Company’s factory
and the costs of travel, room and board if the Company’s employees conduct repair at the Buyer’s location within the
warranty period if the product was not installed by the Company’s and/or is not located within the Company’s service
travel areas.
THE COMPANY DOES NOT MAKE AND EXPRESSLY DISCLAIMS ANY WARRANTY OF MERCHANTABILITY OR
FITNESS FOR A PARTICULAR PURPOSE OR ANY OTHER WARRANTY, EXPRESSED OR IMPLIED, WITH
RESPECT TO THE SALE, INSTALLATION, DESIGN OR USE OF ITS PRODUCTS. ADDITIONALLY, THE
COMPANY SHALL NOT BE LIABLE FOR ANY CONSEQUENTIAL DAMAGES RESULTING FROM THE USE OF OR
ANY DEFECTS IN ITS PRODUCTS.
The Company’s employees are available to provide general advice to customers concerning the use of the Company’s
products; however, oral representations are not warranties with respect to particular products or their uses and may not
be relied upon if they are inconsistent with the relevant product specifications for the items set forth herein.
Notwithstanding the above, the terms and conditions set forth in the Company’s formal sales contracts shall be
controlling and supersede any inconsistent terms contained herein, and any changes to such contracts must be made
in writing and signed by an authorized executive of the Company.
WARNING! THE DISPOSAL AND/OR EMISSION OF SUBSTANCES USED IN CONNECTION WITH
THIS EQUIPMENT MAY BE GOVERNED BY VARIOUS FEDERAL, STATE OR LOCAL
REGULATIONS. ALL USERS OF THIS EQUIPMENT ARE URGED TO BECOME FAMILIAR WITH
ANY REGULATIONS THAT APPLY IN THE USERS AREA CONCERNING THE DUMPING OF
WASTE MATERIALS IN OR UPON WATER, LAND OR AIR AND TO COMPLY WITH SUCH
REGULATIONS.

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Rev 005, 05/11 v
© SP Scientific 2011
Contents
Important Symbols............................................................................................................................................... ii
Safety Warnings...................................................................................................................................................ii
Warranty Information .......................................................................................................................................... iii
Introduction.................................................................................................................1
Overview..............................................................................................................................................................1
Key Features...................................................................................................................................................2
Installation and Startup ...............................................................................................3
Initial Inspection...................................................................................................................................................3
Setup and Installation ..........................................................................................................................................4
Standard Door Removal..................................................................................................................................4
Sample Extractor Door Installation..................................................................................................................5
Operation....................................................................................................................7
SEA Operation.....................................................................................................................................................7
Shelf Area............................................................................................................................................................9
Electrical Requirements...................................................................................................................................9

Contents
Lyo-SEA
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© SP Scientific 2011
Chapter
1
Introduction
Overview The FTS Systems Sample Extractor allows the operator to select and remove a serum
bottle or vial from the trays at any time during the freeze drying run. The selection and
removal process is accomplished with virtually no interruption or alteration of the
drying protocol. The simplicity of the design and ease of use provides operators with a
cost effective methodology for periodic removal of stoppered product containers for
sample analysis. The Sample Extractor is a pivotal option when the operator’s mission
is product development or stability studies.

Introduction
Lyo-SEA
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© SP Scientific 2011
Key Features
The Sample Extractor is mounted on a modified acrylic door of the LyoStar II. It
consists of an:
Acrylic vacuum antechamber•
with stoppering ram•
Acrylic antechamber isolation door•
Vacuum pump•
Inert gas back-fill valve•
Vacuum gauge•
Stainless steel selector arm.•
Vacuum Pump (not shown)
Acrylic Door Vacuum Antechamber
Stoppering Ram
Selector Arm
Inert Gas Backfill/
Vacuum Break Valve
Vacuum Gauge
Isolation Door
Locking Handle
Vacuum Pump Port
Antechamber
Isolation Door
Jaw Controls
Filter
Thumbscrews

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© SP Scientific 2011
Chapter
2
Installation and Startup
Initial Inspection
Your Lyo-SEA tissue-freezing bath was carefully packed and thoroughly inspected
before leaving the SP Scientific factory. However, in the unlikely event that shipping
damage has occurred, retain all packing material and contact your freight carrier
immediately.
DO NOT ACCEPT DAMAGED SHIPMENTS FROM A CARRIER WITHOUT A SIGNED
NOTIFICATION OF DAMAGES.
Upon receiving your shipment, inspect all contents of your equipment for damage.
Uncrate and/or unwrap the unit. Carefully remove all packing material from the unit
and inspect for visible damage. Check packing material for small accessory items.
Inspect the inside of the unit and related parts for visible damage and leaks. Check for
visible liquid at or near the base of the unit. The bath chamber should be clean and
dry.
If concealed damage or loss is discovered, contact the freight carrier immediately.1
Keep all contents, packing material and related paperwork intact until a written report
is obtained.
Note: SP Scientific will cooperate in the matter of collecting your claim, but is not responsible
for the collection or free replacement of the material. When possible, replacement parts will be
shipped and invoiced to you, making them a part of your claim.
1“Concealed damage or loss” refers to damage or loss that does not become apparent until the merchandise has been
unpacked and inspected. Should damage or loss be discovered, you may make a written request for inspection by the carrier's
agent within 15 days of the delivery date. You may then file a claim with the freight carrier or SP Scientific, depending on the
terms of your shipment. If your shipment was “FOB Destination” file your claim with SP Scientific and include the inspection
report and any other supporting documents. If your shipment was “FOB Shipping Point” file your claim with the freight carrier
and include the inspection report and any other supporting documents.

Installation and Startup
Lyo-SEA
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© SP Scientific 2011
Setup and Installation
Note: Field replacement of the standard door, with a Sample Extractor door, requires two
people.
Standard Door Removal
Open the standard door. An assistant should take position to support the1. door.
Remove the upper and lower quick release pins.2.
Note: The quick-release pins may be saved for replacement pins.
Remove the standard door.3.
Quick Release
Pins

Lyo-SEA
Installation and Startup
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© SP Scientific 2011
Sample Extractor Door Installation
Unscrew and remove both thumb screws.1.
Remove the front antechamber plate with selector arm.2.
With help from an assistant, align the Sample Extractor door in the hinge3. brackets.
Re-install the quick release pins.4.
Re-install the front plate with selector arm.5.
Extractor door without front
antechamber cover and selector
arm.
Thumb Screw
Front Plate

Installation and Startup
Lyo-SEA
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© SP Scientific 2011
Chapter
3
Operation
Sample Extractor Assembly Operation
Note: Make sure that the front plate is closed and the inert gas/vacuum break valve is closed.
Activate the auxiliary vacuum pump to decrease the pressure in the1. antechamber.
After the gauge stabilizes at the maximum position, allow the vacuum pump to2. operate for an additional 15 minutes.
Open the antechamber isolation door. With locking handle in the up, or3. unlocked position, the antechamber door can be slid open.
When antechamber isolation door is fully open, lower the handle to lock the4. isolation door in position.
Manipulate the selector arm to select and extract the vial(s) of choice. Vials5. are securely held in spring loaded jaws. Push the button to open the jaws and
release to close them.
Place the sample(s) in the antechamber directly beneath the stoppering ram.6.
Shut the isolation door then lower the handle to seal it closed.7.
Raise handle up to
unlock the door
Push to
open the jaws
Remove shroud
to stopper vials

Operation
Lyo-SEA
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© SP Scientific 2011
Inert gas back fill may be performed at this time if gas is supplied to the8. vacuum break valve.
Remove the shroud from the stoppering ram to manually stopper the vial(s).9.
Open the inert gas fill/vacuum break valve until the antechamber is back to10. atmospheric pressure.
Loosen the thumb screws and slide the front plate down until it can be11. opened.
Remove the stoppered vials.12.
13.
Close the front plate to reseal the chamber for the next run.14.
Lower handle to
lock shut
Press to stopper
Open front plate
to remove the vials

Lyo-SEA
Operation
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© SP Scientific 2011
Shelf Area
Note: Latching one shelf up will position the other shelves so that a 20 ml vial can be used and
increases the accessible shelf area to 240 sq.in. This configuration requires vials to be rotated
90 degrees, to a horizontal position, to be removed.
Electrical Requirements
120V/60hz 6 Amps•
LYO3 (shown below)
The profile shows 190 sq. in. of area that can be
reached on the middle shelf.
The 5 ml vial is the tallest vial
that can be used on a 3 shelf LyoStarII .

Operation
Lyo-SEA
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© SP Scientific 2011

SP Scientific
815 State Route 208
Gardiner, NY 12525 USA
3538 Main Street
Stone Ridge, NY 12484
www.SPScientific.com
[phone]
[phone]