Sper scientific 300033 User manual

DIGITAL REFRACTOMETER
300033, 300034, 300035, 300036
INSTRUCTION MANUAL

2
CONTENTS
Title Page
1. Intro uction ................................................... 2
2. Panel Description .......................................... 3
3. Operating Instructions
3-A. General Measurement......................... 4
3-B. Changing the Temperature Scale ....... 4
3-C. ATC ..................................................... 4
3-D. Calibration Proce ures........................ 4
3-E. Scale Selection.................................... 5
3-F. Range Limit Alert................................. 5
3-G. Zero Checking ..................................... 5
3-H. Battery Replacement........................... 6
3-I. Precautions ......................................... 6
3-J. Erratic Rea ings.................................. 6
4. Specifications ................................................ 7
5. Warranty .......................................... Back Cover
1. INTRODUCTION
This high precision instrument line is esigne especially for
laboratory accuracy. The igital rea out eliminates
uncertainties, while the water resistant esign facilitates clean
ups an protects the electronics from moist environments. All
mo els isplay sample temperature in a ition to their other
parameters. Refractometers operate on the principle that, as
the concentration or ensity of a solution increases, its
refractive in ex changes proportionately. The larger the
concentration of issolve substances in the solution, the
higher the rea ing.
Features inclu e a wi e measurement range, automatic
temperature control (ATC), °C/°F selectable scales, high/low
range limit alerts, auto shut off, an a low battery in icator.

3
1. High/Low Range Limit Alert
2. READ an Enter
3. ZERO an ►
4. SCALE an ▼
5. FUNC an Y/N
6. ON an OFF
7. LCD
8. Prism plate
9. Prism
10. Cover
FACEPLATE &
LCD DISPLAY
1. Prism Temperature
2. ATC (activate
when isplaye )
3. High Limit Alert
4. Low Limit Alert
5. Low Battery
6. Measurement Value
7. Calibration Error
8. Scale In icator
2. PANEL DESCRIPTION
1 2 3 4 5 6 7 8 9 10
Brix% nD
6
7
8
nD
76.4°F ATC H-Limit L-Limit
-1.8.8.8.8 %
▼
READ ZERO SCALES FUNC
ENTER ◄ ▼ Y/N
ON/OFF
1 2 3 4 5

4
3. OPERATING INSTRUCTIONS
3-A. General Measure ent
• To avoi amage, it is important to clean the PRISM
WINDOW before an after use.
• Press the ON/OFF button to turn the Digital Refractometer
on. The unit will automatically shut off after about 3
minutes of inactivity.
• Perform the Calibration Procedures (below).
• With the unit on, fill the PRISM WINDOW with the sample
to be measure , then close the COVER.
• Press the READ button imme iately to ensure accurate
rea ings. Delays may cause the sample to evaporate.
3-B. Changing the Te perature Scale: With the unit on,
press the FUNC button as nee e until l.P C (or F ) is
isplaye . Press the SCALE button to view the temperature
in Fahrenheit (or Celsius). Press the READ button to exit.
3-C. Auto atic Te perature Co pensation (ATC): This
feature makes manual temperature correction unnecessary.
However, to obtain precise rea ings the sample an the unit
shoul be at similar ambient temperatures. ATC appears on
the LCD when this function is activate . To set the ATC
function, press the FUNC button as nee e until ALC.Y (or n)
appears. Press the SCALE button to toggle between on or
off. Press the READ button twice to exit.
3-D. Calibration Procedures
• Clean the PRISM WINDOW before an after every use.
• Fill the PRISM WINDOW with a stan ar solution such as
istille or eionize water. The i eal temperature of the
solution an the unit shoul be 65°~72°F (18°~22°C).
• Close the COVER.
• Press the ON/OFF button to turn the unit on.
• Press the ZERO button for 3 secon s.
• When the proce ure is complete, CAL will be isplaye .
• To check the nD value, press the ZERO button once.
• This ata can be viewe at any time to show the last
calibration value.
• A flashing crosshair symbol is isplaye when the
calibration solution falls outsi e the accepte range.

5
3-E. Scale Selection: Press the SCALE button to toggle
though the scales. The ▼ symbol in icates the active scale.
3-F. High/Low Range Li it Alert: A re light will flash
when the measurement is outsi e of the range limit.
• Setting the High Range Li it Alert
• With the unit on, press the FUNC button as nee e until
H.L Y an the flashing H-Limit icon is isplaye .
• Press the READ button an the last igit flashes.
• Use the ▼ an the ◄ buttons to set the high range limit.
• Press the READ button activate the function.
• Press the FUNC button as nee e to return to the
measurement screen.
• Deactivating the High Range Li it Alert
• Press the FUNC button as nee e until H.L n appears.
• Press the Read button to eactivate the function.
• Press the FUNC button as nee e to return to the
measurement screen.
• Setting the Low Range Li it Alert
• With the unit on, press the FUNC button as nee e until
L.L Y an the flashing L-Limit icon is isplaye .
• Press the READ button an the last igit flashes.
• Use the ▼ an the ◄ buttons to set the low range limit.
• Press the READ button activate the function.
• Press the FUNC button as nee e to return to the
measurement screen.
• Deactivating the Low Range Li it Alert
• Press the FUNC button as nee e until L.L n appears.
• Press the Read button to eactivate the function.
• Press the FUNC button as nee e to return to the
measurement screen.
3-G. Zero Checking: Perform Zero Checking if the unit has
sustaine a shock, is subjecte to temperature changes, or
seems inaccurate.
• Fill the prism win ow with a stan ar solution. This shoul
be the same temperature as the solution to be teste .
• With the unit on, press the ZERO button. A refractive in ex
between 1.3329~1.3331 in icates that the unit is accurate.
• If the unit is out of range, a flashing crosshair symbol
will be isplaye , in icating that recalibration is require .
• Press any button to exit this function.

6
3-H. Battery Replace ent
• When the battery is low, this symbol will be isplaye :
• Open the back cover to replace the 9V battery.
• Reinsert the back cover, making sure it is properly seate .
A built-in gasket protects the circuitry from spills.
Note: The battery provi e with your instrument is primarily
inten e to check that the unit operates properly. The battery
itself is not guarantee an shoul be replace when the low
battery symbol is isplaye .
3-I. Precautions
• Protect the LCD from exposure to strong light sources.
• Avoi prolonge use or storage in extreme temperatures.
• Clean the prism an win ow surfaces before an after use
with a soft wet clean cloth or paper towel. Other surfaces
may be cleane with a mil liqui ish etergent an water.
• Do not use in corrosive environments.
• Do not submerge the unit.
• This precision measuring instrument contains elicate
optics an electronics. Han le with care an o not rop.
• To avoi electric shock, make sure the battery compart-
ment is covere an o not isassemble the unit.
3-J. Erratic Readings
If the rea ings are significantly above or below the expecte
results, consi er the following:
• Sample contains more than one issolve soli . For
example, if the sample contains both salt an sugar, a Brix
refractometer will not isplay the true sugar content
because the salt content will skew the brix measurement.
• When iluting samples, be sure to use a pure solution such
as istille water.
• Sample contains suspen e soli s. Filter an thoroughly
mix the sample before testing.
• Perform a calibration.
• Replace the battery.
• Outsi e light can cause erratic rea ings, make sure to
close the COVER on the PRISM uring measurement.

7
4. SPECIFICATIONS
Models Type Range Res. Accuracy
300033
Brix 45.0~95.0% 0.1% ±0.2%
nD 1.4098~1.5318 0.0001 ±0.001
300034
Brix 0.0~95.0% 0.1% ±0.2%
nD 1.330~1.5318 0.0001 ±0.001
300035
Brix 0.0~60.0% 0.1% ±0.2%
Salinity 0.0~28.0% 0.1% ±0.1%
Salinity
PPT 0~280 1 ±1
300036
USG 1.000~1.090 0.001 ±0.002
Serum
Protein 0.0~12.0 0.1 ±0.1
nD 1.330~1.4098 0.0001 ±0.001
Temperature
(All Mo els)
0~40°C 0.1°C ±1°C
-32~104°F 0.1°F ±2°F
nD 1.3330~1.4419 0.0001 ±0.001
Power Supply: 9V Battery (apx. 5000 tests/battery)
Weight: 18 oz (500 g)
Dimensions: 7" x 4" x 2½" (180 x 100 x 65 mm)
Operating Temp: 41°~95°F (5°~35°C)
Storage Temp: 14°~122°F (-10°~50°C)
Inclu e : Protective Case, 9V Battery, Distille
Water, an a Transfer-Pipette

1 YEAR METER WARRANTY
Sper Scientific warrants this pro uct against efects in
materials an workmanship for a perio of one (1) year from
the ate of purchase, an agrees to repair or replace any
efective unit without charge. If your mo el has since been
iscontinue , an equivalent Sper Scientific pro uct will be
substitute if available. This warranty oes not cover probes,
batteries, or amage resulting from acci ent, misuse, or
abuse of the pro uct. In or er to obtain warranty service,
simply ship the unit postage prepai to:
SPER SCIENTIFIC LTD.
7720 East Re fiel , Suite 7
Scotts ale, Arizona 85260
[phone]
[e-mail]
www.sperscientific.com
Please Note: The efective unit must be accompanie by a
escription of the problem an your return a ress. Please
be sure to return your warranty registration car within ten
(10) ays of purchase.
Rev. 3/10/2011