Sper scientific 300058 User manual

Waterproof Digital
Refractometer
300058, 300059,
300060, 300062,
300063, 300064
& 300065
Instruction Manual

2
Waterproof Digital Refractometer
Copyright ©2017 by Sper Scientic
ALL RIGHTS RESERVED
Printed in the USA
The contents of this manual may not be reproduced
or transmitted in any form or by any means electronic,
mechanical, or other means that do not yet exist or may
be developed, including photocopying, recording, or
any information storage and retrieval system without the
express permission from Sper Scientic.

3
TABLE OF CONTENTS
1. INTRODUCTION . . . . . . . . . . . . . . . . . 4
2. MATERIALS SUPPLIED . . . . . . . . . . . . . 5
3. FEATURES . . . . . . . . . . . . . . . . . . . . 5
4. POWER SUPPLY . . . . . . . . . . . . . . . . . 6
5. FRONT PANEL DESCRIPTION . . . . . . . . . . 7
6. LCD DESCRIPTION. . . . . . . . . . . . . . . . 7
7. CALIBRATION. . . . . . . . . . . . . . . . . . . 8
8. MEASUREMENT PROCEDURES . . . . . . . . 9
9. ERROR CODES. . . . . . . . . . . . . . . . . . 10
10. SCALE INFORMATION . . . . . . . . . . . . .10
11. SPECIFICATIONS . . . . . . . . . . . . . . . . 12
12. WARRANTY . . . . . . . . . . . . . . . . . . . 16

4
INTRODUCTION
Housed in a protective rubberized IP65 Waterproof case
to safeguard the electronics from wet environments and
makes for easy clean ups Model 300058 features a
scratchproof sapphire lens.Measure samples as small
as 1 ml, in the eld with laboratory accuracy. Sample
temperature is initially displayed on the large LCD
followed by the measurement results. The sophisticated
microprocessor provides automatic calibration, automatic
temperature compensation and automatic shuto󰀨.
Powered by a rechargeable, long-life, Li (Lithium Ion)
battery providing 50,000 tests on a single charge.Simple
one-button calibration using distilled water ensures
accurate readings.

5
MATERIALS SUPPLIED
• Refractometer
• One lithium-Ion battery
• DC Power Adapter
• Distilled Water
• Transfer pipet
• Instruction Manual
• Carrying Case
FEATURES
• Displays sample temperature followed by
measurement result
• IP65 Waterproof, rugged exterior
• Rechargeable battery
• Low battery indicator
• DC power adapter
• Automatic shuto󰀨

6
POWER SUPPLY
The meter is powered by one rechargeable lithium-ion
battery. An icon in the upper-right corner of the LCD
indicates the level of battery power available:
75%-100% power
40%-75% power
15%-40% power
Less than 15% power. (This icon will ash)
The DC adapter included plugs into the port on the left
side of the meter. This both provides an alternate power
supply and recharges the battery.
Automatic Shuto󰀨
The power will automatically shut o󰀨 if the meter is not
used for 3 minutes.

7
FRONT PANEL DESCRIPTION
1. POWER - Power on/o󰀨
2. READ - Read measurements /select scale
3. CAL - Calibrate meter
4. LENS COVER
LCD DISPLAY
1. S 1 - Current scale number
2. BATTERY ICON -
Battery power level
3. DISPLAY - Displays temperature
and readings
1 2 3 4

8
CALIBRATION
Use distilled water (refractive index 1.3330 to 1.3340)
to calibrate the meter.
1. With the meter turned o󰀨, place a few drops of
distilled water on the lens.
2. Close the lid to cover the lens.
3. Press POWER to turn the meter on.
4. The temperature of the distilled water appears
on the LCD.
5. Press and hold CAL for 2 seconds to enter
Calibration Mode. “CAL” will be displayed to indicate
that calibration is occurring.
6. When calibration is nished the meter returns to
Measurement Mode and “ - - - ” is displayed on
the LCD.
Note…
For best results, calibrate the meter at 20°C.
Measurements can be taken at other temperatures, but
the calibration should be done at 20°C.

9
MEASUREMENT PROCEDURES
After calibration you can remove the distilled water from
the lens and begin measurement.
Note…
Use a clean, lint-free cloth or tissue to remove solutions
from the lens.
1. With the meter turned o󰀨, place a few drops of the
solution being measured on the lens.
2. Press POWER to turn the meter on.
3. The temperature of the solution appears on the LCD.
4. Close the lid to cover the lens.
5. Press Read to view the measurement on the LCD.
6. To change scales, press and hold Read for 2 seconds
until the scale number changes. Repeat to cycle
through the available scales. (see page 10)
7. Press Read if desired to repeat the measurement.

10
MEASUREMENT ERRORS
Certain conditions can cause an error code to
appear on the LCD:
Error Code & Cause
HHH The measurement is above the upper limit
LLL The measurement is below the lower limit
SCALE INFORMATION
Scale Indicator & Name - 300058
S1 Brix
S2 Refractive Index
S3 Dextran
S4 Fructose
S5 Glucose
S6 Lactose
S7 Maltose
Scale Indicator & Name - 300059
S1 Brix
S2 Refractive Index
Scale Indicator & Name - 300060
S1 Brix
S2 Refractive Index

11
Scale Indicator & Name - 300062
S1 Salinity %
S2 Salinity ‰
S3 Specic Gravity
S4 Refractive Index
Scale Indicator & Name - 300063
S1 Cleaner
S2 Ethylene Glycol
S3 Propylene Glycol
S4 Battery
Scale Indicator & Name - 300064
S1 Urine Specic Gravity
S2 Serum Protein
S3 Refractive Index
Scale Indicator & Name - 300065
S1 ADBLUE
S2 Refractive Index

12
SPECIFICATIONS
Unit of Measure Range Accuracy
Temperature 0.0 °C to 40.0 °C ±0.8 °C
ATC Range 0.0 °C to 40.0 °C ±0.8 °C
Sample Capacity 0.3 mL to 1 mL
Measurement Time 0.5 to 1.0 seconds
Waterproof Rating IP65
Sugars - 300058
Unit of Measure Range Resolution Accuracy
Brix 0.0 to 95% 0.1% ±0.2%
Refractive Index
(nD)
1.3330 to
1.5320 0.0001 ±0.003 nD
Dextran 0.0 to 10.6% 0.1% ±0.2%
Fructose 0.0 to 68.9% 0.1% ±0.2%
Glucose 0.0 to 59.9% 0.1% ±0.2%
Lactose 0.0 to 16.5% 0.1% ±0.2%
Maltose 0.0 to 15.6% 0.1% ±0.2%
Temperature 0.0 to 40°C 0.1°C ±0.8°C

13
Brix 0 to 60% - 300059
Unit of Measure Range Resolution Accuracy
Brix 0 to 60% 0.1% ±0.2%
Refractive Index
(nD)
1.3330 to
1.4420 0.0001 ±0.003 nD
Brix 0 to 95% - 300060
Unit of Measure Range Resolution Accuracy
Brix 0 to 95% 0.1% ±0.2%
Refractive Index
(nD)
1.3330 to
1.532 0 0.0001 ±0.003 nD
Salinity - 300062
Unit of Measure Range Resolution Accuracy
Salinity 0 to 28% 0.1% ±0.2%
0 to 280‰ 1‰ ±2‰
Specic Gravity 1.000 to 1.217 0.001 ±0.002
Refractive Index
(nD)
1.3330 to
1.3900 0.0001 nD ±0.003 nD

14
Automotive - 300063
Unit of Measure Range Resolution Accuracy
*Windshield
Washer Fluid 32 to -40°F 0.1°F ±0.2°F
Ethylene Glycol 32 to -50°F 0.1°F ±0.2°F
Propylene Glycol 32 to -50°F 0.1°F ±0.2°F
Battery 1.000 to
1.500 sg 0.001 ±0.005 sg
Note...
*Windshield Washer Fluid is an aqueous cleaner
used in a vehicle’s automated spray system. It is not
glass cleaner that is applied by hand and wiped with a
towel. Windshield Washer Fluid is a mixture that may
contain any or all of the following: detergent, methanol,
propylene glycol, and silicone polymers. The output of
the refractometer is the freezing point of the windshield
washer uid.

15
Clinical - 300064
Unit of Measure Range Resolution Accuracy
Urine Specic
Gravity 1.000 to 1.050 0.001 ±0.002
Serum Protein
g/dL 0 to 12 0.1 ±0.2
Refractive Index
(nD)
1.3330 to
1.3900 0.0001 ±0.003 nD
Diesel Exhaust Fluid - 300065
Unit of Measure Range Resolution Accuracy
ADBLUE
(Diesel Exhaust
Fluid)
0 to 40% 0.1% ±0.2%
Refractive Index
(nD)
1.3330 to
1.4098 0.0001 ±0.003 nD

16
WARRANTY
Sper Scientic warrants this product against defects
in materials and workmanship for a period of one (1)
year from the date of purchase, and agrees to repair
or replace any defective unit without charge. If your
model has since been discontinued, an equivalent
Sper Scientic product will be substituted if available.
This warranty does not cover probes, batteries, battery
leakage, or damage resulting from accident, tampering,
misuse, or abuse of the product. Opening the meter to
expose its electronics will void the warranty.
To obtain warranty service, ship the unit postage prepaid to:
SPER SCIENTIFIC LTD.
8281 E. Evans Rd., Suite 103
Scottsdale, AZ 85260
The defective unit must be accompanied by a description
of the problem and your return address. Register your
product online at www.sperwarranty.com within 10 days
of purchase.
rev. 7/26/2017