1200C Chilled GenoLyte®
1300-87068-1A, REV 0 SPEX® SamplePrep LLC Page 4 of 16
1.0 INTRODUCTION
The 1200C Chilled GenoLyte® is an efficient, compact laboratory homogenizer that uses a
circulating-chiller (not included) to maintain sample temperature between 0 to 10⁰C while
effectively disrupting cellular materials by horizontally oscillating 2 mL vials. Sample
materials that can be prepared include seeds, stems, roots, leaves, and animal tissue.
This motion allows the mill to be used to prepare sample tissue for extractions of nucleic
acid, proteins, and other constituents by agitating the tissue, steel balls or beads, and a lysis
agent together in each vial.
The isolation of nucleic acids from intact samples requires mechanically disrupting the
samples, followed by the extraction and subsequent purification of the nucleic acid.
Mechanical tissue disruption is often performed manually with a mortar and pestle, an
approach that is not practical for day-to-day screening since manual grinding of tissues is
slow, and re-use of mortars and pestles may lead to cross-contamination. Alternatively,
nucleic acids can be isolated in a vial using balls or beads that mechanically disrupt the
sample. Conventional isolation methodologies can then be used to extract the nucleic acids
from the homogenate.
The features include: modern design, quick-connect cooling setup, operating commands
through a digital timer, large clear lid for visibility of sample loading area, safety interlock,
and the clamp orientation makes it easy to load/unload samples.
Operation is simple: the vials are secured in the sample cooling chamber, and the lid closed.
The controls are checked for the proper running time, rate, and the START button pushed.
When the run is complete, the lid is lifted and the vials are removed.
NOTE: PLEASE DO NOT OPERATE THE GENOLYTE®UNTIL YOU HAVE READ THESE
INSTRUCTIONS AND ARE FAMILIAR WITH ITS CONTROLS AND CLAMP MECHANISM.
THE 1200C CHILLED GENOLYTE®IS INTENDED FOR USE ONLY BY QUALIFIED AND
TRAINED PERSONNEL.
