SPEX SamplePrep 1200C Chilled GenoLyte User manual

1300-87068-1A, REV 0
65 Liberty St., Metuchen, NJ 08840
1-855-GET-SPEX (438-7739) or 732-623-0465
www.spexsampleprep.com
1200C Chilled GenoLyte®
OPERATING MANUAL
For 1200C Chilled GenoLyte®, this manual is Part No. 87068
Patented

1200C Chilled GenoLyte®
1300-87068-1A, REV 0 SPEX® SamplePrep LLC Page 2 of 16
Copyright 2021 by SPEX SamplePrep LLC
All rights reserved. No part of the work may be reproduced or transmitted in any form or
by any means, electronic or mechanical; including photocopying and recording, except as
may be expressly permitted by the 1976 Copyright Act or in writing from the publisher.
Requests for permission should be addressed in writing to SPEX SamplePrep LLC,
65 Liberty Street, Metuchen, NJ 08840.

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TABLE OF CONTENTS
SECTION
DESCRIPTION
PAGE
1.0 Introduction..................................................................................................................4
2.0 Specifications................................................................................................................5
3.0 Unpacking.....................................................................................................................6
4.0 Setting Up .....................................................................................................................7
4.1 Electrical Connection....................................................................................................7
4.2 Cabinet Set-Up..............................................................................................................7
4.3 Standard Safety Features .............................................................................................7
4.4 CE Safety Features........................................................................................................8
4.5 General Safety ..............................................................................................................8
5.0 Operation......................................................................................................................8
5.1 Control Panel ................................................................................................................8
5.2 Changing Settings …………………………………………………………………………………………….……8
6.0 Homogenization ...........................................................................................................9
6.1 Temperature Controlled Homogenization...................................................................9
6.2 Chiller Setting Reference Table 1 ...............................................................................10
6.3 Pre-Loaded Disruption Tubes Reference Table 3.......................................................11
7.0 Chiller Connection ......................................................................................................12
7.1 Description..................................................................................................................12
8.0 Error Messages...........................................................................................................13
8.1 Lid Error.......................................................................................................................13
8.2 Run Fault .....................................................................................................................13
9.0 Maintenance...............................................................................................................13
10.0 Warranty.....................................................................................................................14
10.1 Product Specifications................................................................................................14
10.2 To Arrange a Return Shipment...................................................................................14
11.0 Instrument Disposal....................................................................................................15
12.0 Contact Us...................................................................................................................16

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1.0 INTRODUCTION
The 1200C Chilled GenoLyte® is an efficient, compact laboratory homogenizer that uses a
circulating-chiller (not included) to maintain sample temperature between 0 to 10⁰C while
effectively disrupting cellular materials by horizontally oscillating 2 mL vials. Sample
materials that can be prepared include seeds, stems, roots, leaves, and animal tissue.
This motion allows the mill to be used to prepare sample tissue for extractions of nucleic
acid, proteins, and other constituents by agitating the tissue, steel balls or beads, and a lysis
agent together in each vial.
The isolation of nucleic acids from intact samples requires mechanically disrupting the
samples, followed by the extraction and subsequent purification of the nucleic acid.
Mechanical tissue disruption is often performed manually with a mortar and pestle, an
approach that is not practical for day-to-day screening since manual grinding of tissues is
slow, and re-use of mortars and pestles may lead to cross-contamination. Alternatively,
nucleic acids can be isolated in a vial using balls or beads that mechanically disrupt the
sample. Conventional isolation methodologies can then be used to extract the nucleic acids
from the homogenate.
The features include: modern design, quick-connect cooling setup, operating commands
through a digital timer, large clear lid for visibility of sample loading area, safety interlock,
and the clamp orientation makes it easy to load/unload samples.
Operation is simple: the vials are secured in the sample cooling chamber, and the lid closed.
The controls are checked for the proper running time, rate, and the START button pushed.
When the run is complete, the lid is lifted and the vials are removed.
NOTE: PLEASE DO NOT OPERATE THE GENOLYTE®UNTIL YOU HAVE READ THESE
INSTRUCTIONS AND ARE FAMILIAR WITH ITS CONTROLS AND CLAMP MECHANISM.
THE 1200C CHILLED GENOLYTE®IS INTENDED FOR USE ONLY BY QUALIFIED AND
TRAINED PERSONNEL.

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2.0 SPECIFICATIONS
Type of Mill:
Homogenizer, Bead Beater (temperature controlled by external
chiller)
Chiller:
(Sold Separately)
Minimum Temperature Run Setting: - 5⁰C (23⁰F)
Maximum Temperature Run Setting: 10⁰C (50⁰F)
Compatible Chiller
(Recommended)
Cole-Parmer Polystat Standard (#EW-20873-47)
Cole-Parmer Polystat Digital (#EW-10124-00)
Display:
Digital
Homogenizing
Mechanism:
Grinding balls of stainless steel, silica, or zirconia.
Dimensions
15 in. (38 cm) long x 8 in. (20.5 cm) wide x 11 in. (30 cm) high.
Weight:
24 lbs. (10.9 kg) with external power supply.
Capacity:
Three 2 mL vials (accommodates most standard 2 mL vials)
Clamp Speed:
Fixed Speeds (rpm): 2000, 2500, 3000.
Electrical
Specifications:
CE approved. 115V/230V, 60/50Hz.
Circuit Breaker:
Same as ON/OFF Switch.
Power Cord:
3-prong grounded plug supplied or
2-prong European plug supplied.
Operator is responsible for supplying alternate line cord/plug.
Safety Features:
Interlock prevents mill from running if lid is open.
Run Timer:
Maximum 5 minutes.
Motor:
1/7 HP. Maximum speed 4200 rpm.
Environment:
For indoor use only at a maximum altitude of 6600 ft. (2000 m).
Operate at ambient conditions between 40°F (5°C) and 104°F (40°C),
with maximum relative humidity 80% below 88°F (31°C) decreasing
linearly to 50% at 104°F (40°C). Main supply voltage fluctuations up
to 10% of nominal voltage (115 or 230 volts AC RMS). Pollution
degree 1: none or only dry, conductive pollution occurs.

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3.0 UNPACKING
Inspect the exterior of the packing box and report any visible damage to the carrier.
Remove all packing documents from the exterior of the box, and save them for your
records. Open the top of the shipping box. Remove the packing material and accessories,
and gently lift out the 1200C Chilled GenoLyte® Homogenizer. Visually check the unit for
any damage that may have occurred during shipping. Open the lid, remove packing from
around the clamp mechanism. Tilt the cabinet, and remove the two shipping bolts on the
bottom. They hold the floating baseplate to the cabinet for shipping. Check the packing list
to see that there are no parts missing, and inspect the accessories. We recommend storing
the packaging materials in the event there is a need to return the unit for warranty service
or repairs.
2 mL vials are necessary for processing samples but must be purchased separately. The full
range of preloaded 2 mL vials for the 1200C Chilled GenoLyte® is described in our catalog,
The Handbook of Sample Preparation and Handling, GenoLyte Accessories Manual or
www.spexsampleprep.com.
NOTE: Before operating, remove the shipping bolt from the bottom of the 1200C Chilled
GenoLyte; otherwise the mill may be damaged. The screws should be replaced if the
unit is ever to be shipped or transported.
Figure 1 - Back View
On/Off
Switch
(2) Shipping
Bolts
AC Inlet
Lid
Chiller
Connector
Control
Panel
Figure 2 - Front View
Vial
Chamber

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4.0 SETTING UP
The 1200C Chilled GenoLyte® weighs 24 pounds (10.9 Kg). The lid opens from the front
with the handle in the center. The chiller quick-connectors are located on left and right of
unit (Figure 2). The AC Inlet and On/Off switch are located on the back of the cabinet, as
shown in Figure 1. The control panel is fixed to the front side of the unit (Figure 2).
4.1 Electrical Connection
The detachable power cord should be plugged firmly into its inlet, then into an electrical
outlet. Make sure that the electrical outlet is easily accessible in case it becomes necessary
to unplug the unit. For 115V/60HZ use, a 3-prong outlet. The 230V/50HZ power cord has a
standard European 2-prong plug, but modification by the user may be necessary to meet
local electrical codes.
4.2 Cabinet Set-Up
To open the lid, grasp the white handle and raise the lid to its full upright position. Allow
the lid to rest against the cabinet in its open position. To close, grasp the white handle and
lower the lid until it is fully closed.
4.3 Standard Safety Features
Clamp Locking Tab: The clamp arm is fastened down to firmly fit against the vial lids in the
vial chamber (holder). The clamp arm is pressed downward by user until it clasps the
locking tab (Figure 3). Vial holder only accepts 2 mL vials. When removing the vials, first
unclasp the locking tab from the clamp arm.
Figure 3 - The Clamp (Locking Tab)
Locking Tab
Clamp Arm
2 mL vials

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4.4 CE Safety Features
CE directives require that electricity to the motor and timer be interrupted whenever the lid
is open, and that the clamp must stop completely before the lid is opened.
Safety Interlock: If the RUN button is pressed while the lid is open, the 1200C Chilled
GenoLyte will not start. During operation, if the lid is opened, the motor will immediately
stop, and movement of the clamp mechanism will cease.
4.5 General Safety
5.0 OPERATION
NOTE: Never run the 1200C Chilled GenoLyte Homogenizer with the clamp arm
unfastened or not properly secured. Damage and leakage will result.
5.1 Control Panel
5.1.1 Run Parameter
•Time - Can be set in 5-second increments. Maximum runtime is 5.00 min.
•Rate - Run speeds are fixed at 2000 rpm, 2500 rpm, 3000 rpm.
•Rest - Runtime is inactive between cycles and can be set in 15 second increments up
to 5 minutes.
•Cycles - 1 to 5 (the number of runtimes).
5.2 Changing Settings
Step 1. To set a programmed run push the FUNCTION button to select the Runtime, Rate,
Rest, and Cycles.Press the UP arrow (ᴧ) to increase the selected parameter or
press the DOWN arrow (v) to decrease the parameter.
Step 2. To exit the Function programming screen push the STOP button.
Step 3. Push the START button to begin a programmed run. The timer counts down in 1-
second increments, showing the time left in the run. When the timer reaches zero
and the motor shuts off, the timer will display RUN COMPLETE. The safety
interlock switch prevents the unit from running if lid is open.
Caution: Every effort has been made to ensure the 1200C Chilled
GenoLyte operates at a moderate noise level. The intensity of noise is
directly impacted by the type of grinding vial, type of balls, run speed
(e.g. 3000 rpm) and sample hardness. Ear protection is recommended.

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5.2 Changing Settings (Cont’d)
Step 4. To pause the mill during a run and retain the timer setting, push the PAUSE
button. The motor will shut off and PAUSED will appear on the display. To resume
the run, close lid and push START; the timer will briefly display the exact number of
seconds left in the run and then continue to count down in 1-second increments.
To abort the run, push STOP; RUN COMPLETE will be displayed.
6.0 HOMOGENIZATION
Depending on the sample (e.g. plant, tissue) and amount of material used for the extraction
process, vials with one large or several smaller balls may be more efficient.
In the GenoLyte Accessories Manual, 2 mL vials preloaded with grinding media are listed
with a brief description for suggested sample types. The samples can be ground dry or with
the addition of a lysis buffer. Refer to the supplier extraction kit instructions. Note:
Extraction kits are not included with the 1200C Chilled GenoLyte or sold by SPEX
SamplePrep.
6.1 Temperature Controlled Homogenization
(See manufacturer’s operation manual on how to fill and run chiller.)
The vial cooling chamber (or inside the vial) temperature is typically 1 to 1.5°C higher than
the chiller temperature. For example, setting the chiller temperature to 0°C will result in
the vial chamber temperature being 1 to 1.5°C (the vial will acclimate to this temperature in
approx. 5 seconds).
Note: The grinding media (steel balls, ceramic) will generate some frictional during
homogenization. To control temperature spiking, program a run in cycles with rest (pause)
feature (Section 5.0). Keeping runtimes short (e.g. 30 sec), in combination with two or
three cycles, and 15 second rest periods will maintain the vial temperature range within 3
to 4 degrees of the chiller setting.
Display
Start
Button
Pause
Button
Stop
Button
Parameter
Decrease
Parameter
Increase
Figure 6 - Control Panel

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6.1 Temperature Controlled Homogenization (Cont’d)
6.2 Chiller Setting Reference Table 1 (Guidelines for 1200C Chilled GenoLyte Run Protocol)
CHILLER
SETTING
RUNTIME
RATE
REST
CYCLE
VIAL TEMPERATURE
(during run)
-3°C (26.6°F)
40 seconds
3000 rpm
15 seconds
2
0° to 4°C
-1°C (30.2°F)
35 seconds
3000 rpm
15 seconds
2
3° to 8°C
1°C (33.8°F)
30 seconds
3000 rpm
15 seconds
3
4° to 8°C
3°C (37.4°F)
30 seconds
3000 rpm
15 seconds
3
6° to 10°C
Note: The vials acclimate to the chiller temperature in approximately 5 seconds.
Tip: To improve homogenization and maintain vial temperature increase the number cycles
(1 - 5), keeping the other run parameters (runtime, rate, rest) the same as listed in Table 1.
Some experimentation with grinding media based on sample type is necessary to optimize
homogenization. The Reference Table 6.3 provides a general description of vial sets for
certain sample types. For tough samples use 2310 (2 mL) Reinforced Tube (Table 2.).
Table 2. (Accessories)
2310 (2 mL) Reinforced Tube
Reinforced polypropylene, Self- standing 2 mL microfuge tube with screw-on
polyethylene cap. The nominal outer diameter measures 25/64 in. x 1 27/32 in.
long (10 mm x 47 mm). Grinding load per tube 60 - 120 mg; mixing load 1 mL.
Sold in package of 200.
2151 Grinding Balls, nominal diameter 4/32 in. (3 mm)
Made of 440C stainless steel. Used with reinforced polypropylene tube (#2310).
Sold in package of 100.
2154 Grinding Balls, nominal diameter ¼ in. (6.35 mm)
Made of 440C stainless steel. Used with reinforced polypropylene tube (#2310).
2186 Zirconia Grinding Balls, nominal diameter 15/64 in. (6 mm)
Made of zirconia. Used with reinforced polypropylene tube (#2310). Sold in
package of 1,000.
2302-30 (3 mm) Zirconium Beads
Acid washed zirconium beads, 300 g bottle.

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6.3 Pre-Loaded Disruption Tubes Reference Table 3
PART NUMBER
2 ML VIAL SET
DETAILS
2301-100MB
100 μm Silica Beads (1200 mg)
Economical bead for disrupting
bacteria.
2302-1400AW
1.4 mm Zirconia Beads
Suitable for small tissue samples
and biomass.
2303-MM1
Garnet & ZrO2 Satellites
General Sample Shredding.
2303-MM2
800 micron & 1.4 mm Zirconia
Beads
Mycelium & Soft Leaves.
2303-MM3
100 micron Si, 1.4 mm Zr, & 4
mm Si
Biofilms & Plant Tissues.
2304-100AW
100 μm Silica Beads (600 mg)
Suitable for Bacteria.
2302-100AW2
100 μm Zirconia Beads (600 mg)
Zirconium beads are of higher
density; excellent for bacterial
disruption.
2304-400AW
400 μm Silica Beads
Size is ideal for yeasts such as
Saccharomyces.
2304-800AW
800 μm Silica Beads
Size is suitable for molds and
pollen.
2302-1000AW
1.0 mm Zirconia Beads
Beads are suitable to disrupt
finer soils.
2302-1700AW
1.7 mm Zirconia Beads
Effective for larger tissue
samples and fine plant
materials.
2305-2800SS
2.8 mm Stainless Steel Grinding
Balls
Most dense of all the grinding
media, popular for their
moderate cost. Good for tissues.
2302-3000AW
3.0 mm Zirconia Beads
Good for larger tissue samples.
Excellent chemical resistance to
organics.
2302-6000AW
6 mm Ceria Stabilized Zirconia
Beads
Ideal for grinding tough plant
samples.
2302-200AW
200 µm Zirconium Beads
Suitable for bacteria and small
yeast (e.g. Pichia).

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7.0 CHILLER CONNECTION
7.1 Description
The chiller connection assembly is attached to the 1200C, fitted with two female valved
coupling body for the inlet and outlet chiller ports, as shown in Figure 7. Each female
coupling body has a quick release mechanism to detach male valved coupling (hose). To
attach hose by inserting male valve into female coupling until it snaps in place, locking the
two pieces together.
Connecting to the chiller, two (¼ in. ID) 42 in. flexible hoses are attached to the barb-side
male valved coupling. The inlet port to the chiller is attached to the right side of the 1200C
Chilled GenoLyte (right chiller connection) and the left chiller connection is attached to the
outlet port from the chiller. The two (¼ in. ID) 42 in. specially designed chiller hoses fitted
with a male valved coupling (# 1200C-10) can be purchased from SPEX.
Figure 7 - Chiller Connection (left side view)
Quick Release Button
Quick Release Button
¼ Hose (to chiller)
Chiller Connection
Assembly
Male Coupling

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8.0 ERROR MESSAGES
8.1 Lid Error
If the lid interlock fails while the 1200C Chilled GenoLyte is running, the unit will stop
running and the screen will display a “LID OPEN” message. The timer will maintain the time
remaining in the run. To restart, close the lid and press the START button to finish the run.
To end the run and reset the timer, press the STOP button. The “LID OPEN” message will
disappear once the lid is closed.
8.2 Run Fault
If a “RUN FAULT” message appears on the display screen, this indicates the 1200C Chilled
GenoLyte is not operating within an acceptable range of the set rate. Press the STOP
button to discontinue operation and contact SPEX SamplePrep Service Technician at 1-855-
GET SPEX (732-623-0465) for assistance.
9.0 MAINTENANCE
NOTE: Always unplug the 1200C Chilled GenoLyte before any cleanup or maintenance
work.
The 1200C Chilled GenoLyte® has been designed to provide trouble-free operation over a long
period of time. To assure proper performance, the most important factor is cleanliness. Any
spills should be wiped up immediately.
The cabinet is made primarily of painted plastic. The lid is a clear formed polycarbonate.
The interior as well as the exterior surfaces of the unit are designed to be easily cleaned in
case of a sample spill. To maintain the overall appearance of the unit, periodically wipe the
exterior and interior of the 1200C Chilled GenoLyte with a mild window cleaner or similar
product (use a soft non-abrasive cotton cloth).

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10.0 WARRANTY
SPEX SamplePrep LLC guarantees its products against defects in materials or workmanship
for one year from the date of original shipment. Repairs, replacements, or parts are
guaranteed for 30 days or for the remaining original warranty period (whichever is greater)
for the item that was repaired or replaced. Items not produced by SPEX SamplePrep LLC
carry the manufacturer’s warranty only.
The warranty excludes wear parts. These are parts that wear out through use and must be
replaced periodically for proper operation. The 1200C Chilled GenoLyte wear parts include
the following which can be changed by the user.
21609 Spadebolt
52160 Spring
50157 Vibration Mount
In the event that these or other parts require service, please contact SPEX SamplePrep to
arrange a return shipment.
The customer pays return freight for warranty claims. If the warranty claim is valid, SPEX
SamplePrep will pay return freight to the customer. However, SPEX SamplePrep reserves
the right to judge whether a malfunction during the warranty period is due to defects in
materials or workmanship, or to wear, negligence, or misuse.
10.1 Product Specifications
Every effort has been made to provide complete and accurate product operation and
information in this manual. However, since specifications are subject to change without
notice, changes may be made from time to time to improve the performance of the
product. Therefore slight changes that are not reflected in the current illustrations should
be considered minor and inconsequential for the purposes of this operating manual.
10.2 To Arrange A Return Shipment
Customer satisfaction is important to SPEX SamplePrep. Please bring any problem to our
attention, but please DO NOT RETURN any item before contacting us for a Return
Authorization Number and instructions. Unauthorized returns will be refused. Cost for all
return transportation is the responsibility of the customer. Credit for returned merchandise
will be issued only after goods have been received and inspected. Returned goods are
subject to a 25% restocking charge up to a maximum of $200.00.

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11.0 INSTRUMENT DISPOSAL
In accordance to the EU Directive 2012/19/EU covering Waste Electrical and Electronic
Equipment, all equipment with the disposal symbol must not be disposed of with general
waste. (See Figure 8)
Throughout the European Community, guidelines regarding disposal regulations may vary
from territory to territory. Please contact the national legislation or local authority for
more information on proper disposal of all equipment with this symbol.
Figure 8 - Disposal Symbol
Disposal Label is located
on the back of unit.

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12.0 CONTACT US
Within the United States, write, telephone, or e-mail us at:
SPEX SamplePrep LLC
65 Liberty St.
Metuchen, New Jersey 08840
Tel.: 1-855-GET-SPEX (438-7739) or 732-623-0465
Fax: [phone]
Website: www.spexsampleprep.com
E-mail: [email protected]om
Outside the United States and Europe, contact the SPEX SamplePrep representative from
whom you purchased your equipment. A list of our current representatives is on our
website, www.spexsampleprep.com.