Thermo Scientific AI 1310 User manual

Thermo Scientific
AI 1310 / AS 1310
Autosamplers
User Guide
31716000 Third Edition December 2015

© 2015 Thermo Fisher Scientific Inc. All rights reserved.
AI 1310, AS 1310, TRACE 1300, TRACE 1310, TRACE GC Ultra, FOCUS GC, Xcalibur, Chromeleon,
Chrom-Card and ChromQuest are trademarks of Thermo Fisher Scientific. EzChrom is a trademark of Agilent
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Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
Tel: [phone]; Fax: [phone]
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
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document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history: First edition, released March 2012 “Original Instructions”
Second Edition, released August 2013; Third Edition December 2015
For Research Use Only. Not for use in diagnostic procedures.

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Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
•installation
•re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, 20090 Rodano - Milan - Italy — Tel: [phone] - Fax: [phone]
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety
standards.
Safety
This device complies with EN 61010-1:2001, EN 61010-2:2002 according to Low Voltage Directive 2006/95/EC.
Electromagnetic Compatibility
This device complies with EN 61326-1:2006 according to directive 2004/108/EC.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2012/19/EU. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU)
Member State, and these companies should dispose of or recycle this product. See www.thermoscientific.com/rohsweee for
further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country.
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Questo prodotto è marcato con il seguente simbolo in conformità alla direttiva europea 2012/19/EU (RAEE) sui rifiuti di
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para una mayor información sobre la conformidad de Thermo Fisher Scientific con estas Directivas y para las empresas de
reciclaje en su país.

Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide ix
P
Preface
This manual contains descriptions of the features and components of the AI 1310/AS 1310
sampling systems. Inside, you will find all of the information necessary for routine operation
of your sampling system. This includes operating procedures, sample injection techniques,
and diagrams and descriptions of the major components.
This manual is organized as follows:
•Chapter 1, “Introduction,” provides a basic overview of the features and options of the AI
1310/AS 1310 System. It also describes the available instrument configurations.
•Chapter 2, “Sampling Unit,” provides a description of the components of the sampling
unit of the AI 1310/AS 1310 System.
•Chapter 3, “Installation,” contains the instructions for the installation of the
AI 1310/AS 1310 on the TRACE 1300, TRACE 1310, TRACE GC Ultra and FOCUS
GC gas chromatographs, the syringe and the electrical connections with the different
units of the gas chromatographic system.
•Chapter 4, “AI 1310/AS 1310 Control,” provides the information for controlling the
AI 1310/AS 1310 through the Chromatography Data System, the TRACE GC Ultra, or
the FOCUS GC. The chapter contains also the working procedures with different
injectors.
•Chapter 5, “Maintenance,” provides guidelines for the maintenance of the AI 1310/AS
1310.
•Glossary contains definitions of terms used in this manual. This also includes
abbreviations, acronyms, metric prefixes, and symbols.
•Index contains an alphabetical list of key terms and topics in this guide, including cross
references and the corresponding page numbers.

Preface
About Your System
xAI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
About Your System
Environmental Conditions
•Internaluse
• Up to 2000 meters altitude
• Temperature 18 to 30 °C
• Maximum relative humidity between 30% and 85%
• Voltage variations must not exceed the nominal voltage by ± 10%
• Transient overloads in compliance with installation categories II
• Pollution degree according to IEC 664 (3.7.3) 2
• Protection degree IP00
WARNING Thermo Fisher Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment may be impaired. If you
maintain a system outside the specifications listed in this guide, failures of many types,
including personal injury or death, may occur. The repair of instrument failures caused by
operation in a manner not specified by the manufacturer is specifically excluded from the
Standard Warranty and service contract coverage.
WARNING When, for technical reasons, it is necessary to work on instrument parts
which may involve an hazard (moving parts, components under voltage, etc.) the
authorized Technical Service must be contacted. This type of situations can be identified
because access to these parts is possible only by using a tool. The removable protective
covers bear a warning symbol suggesting to refer to the documentation accompanying the
instrument. Should an operator perform a maintenance operation, he/she must have
received proper training to carry out that specific action.
WARNING Before using dangerous substances (toxic, harmful, and so forth), read the
hazard indications and information reported in the Safety Sheet supplied by the
manufacturer referring to the relevant CAS (Chemical Abstract Service) number.
The AI 1310/AS 1310 sampling system requires the use of several chemical products,
which are present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to the
relevant CAS (Chemical Abstract Service) number.

Preface
Rating
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xi
Rating
AI 1310/AS 1310 Autosampler
• 24 Vdc through a portable external power supply, level VI efficiency
−input 90-264 Vac; 47-63 Hz — output 24 Vdc; 70 W minimum
Contacting Us
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.
Use http://www.thermoscientific.com address for products information.address for products
information.
Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to
contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer Support.
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this guide. Safety and other
special notices appear in boxes and include the following:
WARNING This is the general warning safety symbol and safety alert word to prevent
actions that could cause personal injury. It highlights hazards to humans or the
environment. Each WARNING safety alert is preceded with this safety symbol and another
appropriate safety symbol (see “Safety Alerts and Important Information” on page xi)
Then it is followed with an appropriate safety precautionary message. When you see a
safety alert on your instrument or in the publications, please carefully follow the safety
instructions before proceeding.
CAUTION This is the safety alert word to prevent actions that may cause personal injury or
instrument damage. We use it to highlight information necessary to prevent personal
injury or damage to software, loss of data, or invalid test results; or might contain
information that is critical for optimal system performance. A CAUTION safety alert is
always preceded with an appropriate safety symbol (see “Safety Alerts and Important
Information” on page xi) Then it is followed with an appropriate safety precautionary
message. When you see a safety alert on your instrument or in the publications, please
carefully follow the safety instructions before proceeding.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.

Preface
Safety Alerts and Important Information
xii AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury and/or instrument damage. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION in intended to prevent improper actions
that may cause personal injury and/or instrument damage. The following safety symbols may
be found on your instrument and/or in this guide:
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.
Symbol Descriptor
BIOHAZARD: Indicates that a biohazard will, could, or might occur.
BURN HAZARD: Alerts you to the presence of a hot surface that could or might
cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.
FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.
EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates
this risk could or might cause physical injury.
FLAMMABLE GAS HAZARD. Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
CLOTHING REQUIRED. Indicates that you should wear a work clothing when
performing a task or else physical injury could or might occur.
BOOTS REQUIRED. Indicates that you must wear boots when performing a
task or else physical injury could or might occur.
MATERIAL AND EYE HAZARD. Indicates you must wear eye protection when
performing a task.
HAND AND CHEMICAL HAZARD: Indicates that chemical damage or physical
injury could or might occur.

Preface
Instrument Markings and Symbols
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xiii
Instrument Markings and Symbols
Table 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on the AI 1310/AS 1310. See the asterisk.
HARMFUL. Indicates that the presence of harmful material will, could, or
might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.
LIFTING HAZARD. Indicates that a physical injury could or might occur if two
or more people do not lift an object.
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for a
physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.
LASER HAZARD. Indicates that exposure to a laser beam will, could,or might
cause personal injury.
RADIOACTIVE HAZARD. Indicates that the presence of radioactive material
could or might occur.
For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard. In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
*Alternating Current
Both direct and alternating current
Three-phase alternating current
3

Preface
Safety Information and Warnings
xiv AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Safety Information and Warnings
This safety guide raises awareness of potential safety issues and general points for
consideration for Thermo Fisher Scientific representatives during installation, and repair of
the AI 1310 /AS 1310 sampling system., or parts of it (following the life cycle principle), as
well as for the end user AI 1310 /AS 1310 sampling system in the lab during the learning
phase, and in routine work.
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
*On (Supply)
*Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
*Instruction manual symbol affixed to product. Indicates that the user must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock
*Caution, hot surface
*Caution, biohazard
In-position of a bistable push control
Out-position of a bistable push control
*Jack socket
*Symbol in compliance to the Directive 2012/19/EU on Waste Electrical
and Electronic Equipment (WEEE) placed on the European market after
August, 13, 2005.
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
+-

Preface
Safety Information and Warnings
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xv
General Considerations
• Before a unit is put to use, consult the current User Guide and related documents under
all circumstances.
• Changes or modifications to this unit not expressly approved by the party responsible for
compliance, could void your’s authority to operate the equipment.
• Be aware that if the equipment is used in a manner not specified by the manufacturer, the
protective and safety features of the equipment might be impaired.
• The repair of instrument failures caused by operation in a manner not specified by the
manufacturer is expressly excluded from the standard warranty and service contract
coverage.
• When, for technical reasons, it is necessary to work on instrument parts which may
involve an hazard (moving parts, components under voltage, etc.) the authorized
Technical Service must be contacted. This type of situations can be identified because
access to these parts is possible only by using a tool. The removable protective covers bear
a warning symbol suggesting to refer to the documentation accompanying the
instrument. Should an operator perform a maintenance operation, he/she must have
received proper training to carry out that specific action.
• Before using dangerous substances (toxic, harmful, and so forth), read the hazard
indications and information reported in the Safety Sheet supplied by the manufacturer
referring to the relevant CAS (Chemical Abstract Service) number.
The AI 1310/AS 1310 sampling system requires the use of several chemical products,
which are present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to the
relevant CAS (Chemical Abstract Service) number.
Electrical Hazards
Every analytical instrument has specific hazards. Be sure to read and comply with the
following pre-cautions. They ensure the safe and long-term use of your AI 1310 /AS 1310
Autosamplers.
The installation over-voltage category is Level II. The Level II category pertains to equipment
receiving its electrical power from the local level, such as an electrical wall outlet.
Connect the AI 1310/AS 1310 sampling system only to instruments complying with IEC
61010 safety regulations.
IMPORTANT Read this section first before operating the AI 1310 /AS 1310 Autosamplers.

Preface
Safety Information and Warnings
xvi AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
The power line and the connections between the AI 1310/AS 1310 sampling system and
other instruments, used in the configuration setup of the total analytical system, must
maintain good electrical grounding. Poor grounding represents a danger for the operator, and
might seriously affect the performance of the instrument.
Do not connect the AI 1310/AS 1310 sampling system to power lines that supply devices of a
heavy duty nature, such as motors, refrigerators and other devices that can generate electrical
disturbances.
Pay attention not to leave any cable connecting the AI 1310/AS 1310 sampling system and
the chromatographic system, or the power cord close to heated zone, such as the injector or
detector heating blocks, or the GC hot air vents.
Always replace any cable showing signs of damage with another one provided by the
manufacturer. Safety regulations must be respected.
Do not change the external or internal grounding connections. Tampering with or
disconnecting these connections could endanger you and damage the AI 1310/AS 1310
sampling system.
The instrument is properly grounded in accordance with these regulations when shipped.
To ensure safe operation, you do not must make any changes to the electrical connections or
the instrument's chassis.
Do not turn the instrument on if you suspect that it has incurred any type of electrical
damage. Instead, disconnect the power cord and contact a Thermo Fisher Scientific
representative for a product evaluation. Do not attempt to use the instrument until it has been
evaluated. Electrical damage might have occurred if the AI 1310/AS 1310 sampling system
shows visible signs of damage, exposure to any liquids or has been transported under severe
stress.
Damage can also result if the instrument is stored for prolonged periods under unfavorable
conditions: for example, subjected to heat, moisture, and so on. Ensure that the power
supply/controller unit is always placed in a clean and dry position. Avoid any liquid spills in
the vicinity.
Before attempting any type of maintenance work, always disconnect the power cords from the
power supply if optional devices are installed. Capacitors inside the instrument might still be
charged also if the instrument is turned off.
To avoid damaging electrical parts, do not disconnect an electrical assembly while power is
applied to the AI 1310/AS 1310 sampling system. After the power is turned off, wait
approximately 30 seconds before you disconnect an assembly.
The instrument includes a number of integrated circuits. These circuits might be damaged if
exposed to excessive line voltage fluctuations, power surges or electrostatic charges, or both.
Never try to repair or replace any components of the instrument without the assistance of a
Thermo Fisher Scientific representative. There are no operator-serviceable or replaceable parts
inside the power supply or in the AI 1310/AS 1310 sampling system. If a power supply is not
functioning, contact a Thermo Fisher Scientific representative.

Preface
Safety Information and Warnings
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xvii
Other Hazards
To avoid injury and possible infection through contamination during AI 1310/AS 1310
sampling system operation, keep your hands away from the syringe.
Danger of crushing to fingers and hands. To avoid injury keep your hands away from moving
parts during operation. Turn off the power to the AI 1310/AS 1310 sampling system if you
must reach inside a mechanically powered system with moving parts.
To avoid injury, observe safe laboratory practice when handling solvents, or operating the AI
1310/AS 1310 sampling system. Know the physical and chemical properties of the solvents
you use. See the MSDS (Material Safety Data Sheets) from the manufacturer of the solvents
being used.
When using the AI 1310/AS 1310 sampling system, follow the generally accepted procedures
for quality control and method development.
Do not operate on the instrument components that form part of the work area of the
AI 1310/AS 1310 sampling system when it is in motion.
Do not use vials without a sealing cap without a plate seal. Vapor phase from organic solvents
can be hazardous and flammable. Acidic vapor phase can cause corrosion to critical
mechanical parts.
Do not reuse the vials. During the process of washing the vial, micro-cracks can form which
will weaken the glass wall and increase the chances of the vial breaking.
Working with Toxic or other Harmful Compounds
Before using dangerous substances (toxic, harmful, and so on) read the hazard indications and
information reported in the Material Safety Data Sheet (MSDS) supplied by the
manufacturer, referring to the relevant CAS (Chemical Abstract Service) number. AI 1310/AS
1310 sampling system requires the use of several chemical products with different hazard
characteristics, which are present in vials and syringes. Before using these substances or
replacing the syringe, please read the hazard indications and information reported in the
MSDS supplied by the manufacturer referring to the relevant CAS number.
WARNING Before using hazardous substances (toxic, harmful, and so on), please read the
hazard indications and information reported in the applicable Material Safety Data Sheet
(MSDS). Use personal protective equipment according to the safety requirements.

Preface
Safety Information and Warnings
xviii AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
When preparing the samples, please refer to local regulations for the ventilation conditions of
the work room.
All waste materials must be collected and eliminated in compliance with the local regulations
and directives in the country where the instrument is used.
Maintenance
Any external cleaning or maintenance must be performed with the AI 1310/AS 1310
sampling system turned off and the power cord disconnected. Avoid using solvents and
spraying on electrical parts. For the removal of potentially dangerous substances (toxic,
harmful, and so on) read the hazard indications and information reported in the MSDS
(Material Safety Data Sheet) supplied by the manufacturer referring to the relevant CAS
(Chemical Abstract Service) number. Use proper protective gloves.
When working with hazardous materials such as radioactive, biologically hazardous material,
and so on, it is important to train all operators how to respond in case of spills or
contamination.
Depending on the class of hazardous material, the appropriate measures have to be taken
immediately. Therefore, the chemicals or solvents needed for decontamination have to be on
hand.
Any parts of the equipment which can potentially be contaminated, such as the sample vial
rack, syringe tool, wash module, and so on, must be cleaned regularly. The waste solvent from
cleaning and any hardware which requires to be disposed of has to be properly eliminated
with all the necessary precautions, abiding by national and international regulations.
When preparing for decontamination, ensure that the solvent or chemical to be used will not
damage or react with the surface, dye (color) of the instrument, table or other nearby objects.
If in doubt, please contact your Thermo Fisher Scientific representative to verify the
compatibility of the type or composition of solvents with the AI 1310/AS 1310 sampling
system.
It is your responsibility to handle hazardous chemicals or biological compounds, including
(but not limited to) bacterial or viral samples and the associated wastes, safely and in
accordance with international and local regulations.
Disposal
Do not dispose of this equipment or parts thereof unsorted in municipal waste.
Follow local municipal waste regulations for proper disposal provisions to reduce
the environmental impact of waste electrical and electronic equipment (WEEE).
European Union customers: Call your local customer service representative responsible for the
AI 1310/AS 1310 sampling system for complimentary equipment pick-up and recycling.

Preface
Hazardous Substances Precautions
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xix
Hazardous Substances Precautions
WARNING The customer has to ensure that the AI 1310/AS 1310 sampling system has
not been contaminated by any hazardous chemical or biological compounds including
(but not limited to) bacteria or viruses.
Any part which had direct contact with the analytical sample must be identified and must
undergo an appropriate decontamination procedure prior to shipping for disposal.
Potentially dangerous components are: syringes and vials. Any critical parts sent for
disposal must be handled according to national laws for hazardous compounds.
The customer and the service engineer are fully responsible for enforcing these
requirements. Thermo Fisher Scientific will hold the representative, customer responsible,
or both, if these regulations are not observed.
WARNING Before using hazardous substances (toxic, harmful, and so on), read the
hazard indications and information reported in the applicable Material Safety Data Sheet
(MSDS.) Use Personal protection according to the safety requirements.