SYSMEX SP-50 User manual

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
Routine Use Training Workbook
SP-50
Date:
26/10/2020
Document Ref:
SUKBMS-24-334
Version:
4.0
Classification:
Unrestricted
IFU version:
1801

Disclaimer –SP-50
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
2
Contents
Contents 2
Disclaimer 4
Revision History 4
Reference Documents 4
SP-50 Overview 5
Analyser components 6
Outside the analyser 6
Front Components 8
Smear Unit 9
Stain Unit 10
Reagents On-board the SP-50 11
Smear Production 12
Staining Principles 13
SP-50 –IPU Layout 14
Main Screen 14
Status Area 15
Instrument Status 15
Indicator 16
Slide Preparation Mode 16
Sample Number 16
Sampler/Manual 17
Mode 17
Quick Guide: Maintenance 18
Daily Maintenance 19
Performing Shutdown 1 19
Wipe Stain Baths 21
Performing Start-up 22
Weekly Maintenance 24
Cleaning the Spreader Glass 24
Cleaning the Smear/Stain Unit 27
Cleaning the Stain Pool 30
Monthly Maintenance 31
Performing Shutdown 2 31
As Required Maintenance 34
Loading Glass Slides 34
Replace Spreader Glass 35

Disclaimer –SP-50
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
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Replacing Ink Ribbon 39
Replacing Reagents –CellPack DCL, Stains & Buffer Solution 42
Replacing Methanol 46
Quick Guide: Running Samples 47
Sample Preparation 47
Sampler Preparation 47
Manual Preparation 47
Sample Volumes 48
Sampler Mode 49
Smearing Mode 50
Staining & Smearing Mode 50
Operation of the SP-50 on the XN Track 51
Placing into ‘Offline’ or Isolation 51
Running Samples in ‘Offline’ or Sampler Mode 51
Manual Mode 52
Manual –Smearing & Staining 52
Manual –Stain Only 56
Manual –Smear Only 59
Manual –Print Only 62
Responding to Alarms 65
Audit Logs 66
Contact Us 69

Disclaimer –SP-50
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
4
Disclaimer
Please note the information in this presentation, workbook or training session provided by Sysmex
should not be used as an alternative to your sites Standard Operating Procedure (SOP)/Contract. If you
have any particular questions regarding any site specific use of reagents, consumables and/or
equipment please contact your Management Team.
Revision History
Revised section
Alteration
Name
Date
Daily maintenance
Added wipe stain bath
K. Elgerton
January 2019
Weekly maintenance
Amended weekly schedule
K. Elgerton
January 2019
Reagents
Amended changing stain
K. Elgerton
January 2019
Audit logs
Added exporting CSV
K. Elgerton
January 2019
Reagents
Amended changing Buffer
K. Elgerton
January 2020
Front cover
New logo added
K. Elgerton
January 2020
Contacts
Reagent hotline updated
K. Elgerton
January 2020
Maintenance
Shutdown times updated
K. Elgerton
January 2020
Title page
Workbook name changed to
include routine use.
N. Bowen
October 2020
Reference Documents
Document title
Version
Date
SP-50_BO (IFU)
1801
January 2018
SP-50_GI (IFU)
1801
January 2018
SP-50_TS (IFU)
1801
January 2018

Reference Documents –SP-50 Overview
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
5
SP-50 Overview
Facts and Figures
SP-Series analysers
SP-1000i
SP-10
SP-50
Throughput
SP-50 S –30 samples/hr
SP-50 H –75 samples/hr
Capacity
Standalone analyser –50 samples
Tracked analysers –racks are automatically sent to the SP if a
smear is requested.
Sample Type
Whole blood
Sampler Mode
Regular tube
Raised bottom tube (RBT) –requires RBT rack
Manual Mode
Manual (Closed)
Manual (Open)
Manual (RBT)
Manual (Micro)
Aspiration Volumes
Sampler mode - 70µl
Manual (Closed) mode - 70 µl
Manual (Micro) mode - 38 µl
Staining Protocols
May-Grunwald-Giemsa stain
Wright single stain
Wright-Giemsa stain

Analyser components –SP-50 Overview
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
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Analyser components
Outside the analyser
1. Main Power Switch Cover –Open this cover to turn the main power switch ON/OFF, and to
regulate the pressures if required.
2. Stain Unit Cover –Open thiscover to inspect the interior of the stain unit. Accesscan be gained
for cleaning or maintenance tasks. Locked during operation.
3. Start-up (Power) Switch –Press to start up the instrument.
4. Eject Switch –Press to eject magazines from the stain unit.
5. IPU Touchscreen –Use to operate the instrument and configure settings.
6. Sample Tube Holder –Used to set samples for manual analysis.
7. Mode Switch –Press to switch between manual analysis and sampler analysis. Pressing it
opens and closes the sample holder.
•When the sample holder is open: Manual preparation
•When the sample holder is closed: Sampler preparation

Analyser components –SP-50 Overview
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
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8. Start Switch –Press to start manual analysis.
9. Status Indicator LED - Shows the current status of the analyser.
LED Indicator Light
Description
Green
Ready (Analysis or maintenance is possible)
Flashing Green
Starting up / Analysis in progress / Maintenance in progress
Orange
Sample analysis stopped / not possible. An error has occurred, but the
instrument is still operational.
Flashing Orange
Starting up/Preparing a slide/Switching the slide preparation mode/ Shutting
down/Sleeping
Red
Stopped - Error (without alarm) / initialising system / Analyser ready to be
turned off at the end of shutdown
Flashing Red
Stopped - Error(with alarm)
Not Lit
Powered OFF
10. Manual Magazine Holders - This is used for loading manual slides into the analyser for manual
staining.
11. Lower Front Cover - Open this cover to inspect the interior of the main unit to perform cleaning
or maintenance tasks. Analyser must be turned off.
12. Manual Magazine Holder Status LED –Shows the current status of the manual magazine
holder. A magazine cannot be loaded or removed while the LED is flashing.
LED Indicator Light
Description
Green
Containing magazine
Red
Error
Not Lit
Powered OFF

Analyser components –SP-50 Overview
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
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13. Slide Unit Cover - Open to load glass slides. Locked during operation.
14. Smear Unit Cover - Open this cover to inspect the interior of the smear unit to perform cleaning
or maintenance tasks. The smear unit cover and the slide set cover are one piece. To open the
smear unit cover, remove the slide supply cassette first. Locked during operation
Front Components
1. 0.07 MPa Regulator –Regulates the source pressure to 0.07 MPa
2. Main Power Switch –Press to turn the main power of the main unit on/off
3. Slide Supply Cassette Status LED –Shows the current status of the supply cassettes. A
cassette cannot be loaded or removed while the LED is flashing.
4. Printer –Prints sample information and patient demographics onto glass slide.
5. Slide Supply Cassettes –Loads/stores glass slides for use on the SP-50.
LED Indicator Light
Description
Green
Slides loaded
Red
No slides/error
Not Lit
Powered OFF

Analyser components –SP-50 Overview
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
9
Smear Unit
1. Smear Unit –Spreads the dispensed sample onto glass slide. Contains spreader glass unit.
2. Print Unit –Prints sample information and patient demographics onto glass slide. Contains the
ink ribbon.
3. Smear Dryer Unit –Dries the smeared sample.
4. Slide Supply Unit –Takes out glass slides from the supply cassettes.

Analyser components –SP-50 Overview
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone [phone] · Fax [phone]
[e-mail]· www.sysmex.co.uk
10
Stain Unit
A. Stain Unit Cover –Open thiscover to inspect the interior of the stain unit. Accesscan be gained
for cleaning or maintenance tasks. Locked during operation.
B. Stain Unit –Depending upon protocol; fixes, stains, rinses and dries the prepared slides.
There are 7 staining pools which are filled up with the following reagents. The types of reagent varies
depending on the staining method. Samples move through the staining pool in the direction of the arrow
as illustrated above.
Stain Pool
Role
Reagent
1*
Fixing
Methanol
2
Fixing
Stain 1
3
Staining
Stain 1/buffer
4
Rinsing
Rinse
5
Staining
Stain 2/buffer
6
Rinsing
Rinse & dry
*Use depends upon protocol
A
B
A