SYSMEX SF-3000 User manual

SF-3000/06.95
OPERATOR’S MANUAL
AUTOMATED HEMATOLOGY ANALYZER
SF-3000
SYSMEX CORPORATION
KOBE, JAPAN
Copyright © 1995 - 2001 by SYSMEX CORPORATION
All rights reserved. No part of this Operator’s Manual may be Code No. 461-2459-3
reproduced in any form or by any means whatsoever without PRINTED IN JAPAN
prior written permission of SYSMEX CORPORATION. Date of Last Revision: March 2001

Revised September 1998
Sysmex is a registered trademark of SYSMEX CORPORATION.
CELLPACK, CELLCLEAN, STROMATOLYSER, SULFOLYSER, SF CHECK are
trademarks of SYSMEX CORPORATION.
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Other trademarks referenced are property of their respective owners.
Patient names and doctor names are entered for information and illustration purposes
only, and do not imply real specific persons.

Sysmex SF-3000 Operator's Manual -- Revised September 1995
RECEIVING INSTRUCTIONS
The SF-3000 has been thoroughly tested before shipment, and has been packaged
carefully to prevent damage from shipping and handling. Reagents and options have
also been sent and will arrive at approximately the same time as the analyzer. Follow
these guidelines when the system arrives:
• Check to see that the arrows on the sides of the packages are pointing up. If the
arrows do not point up, remark this information on the bill of lading.
• Visually inspect the outside of the package for rips, dents, or possible shipping
damage. Document any sign of damage on the bill of lading, regardless of how
insignificant it may appear. This is for your protection!
• Notify your service representative that the SF-3000 system and its components
have arrived.
• Wait for your service representative to unpack the system and open the packages.
• Follow the unpacking and storage instructions provided on the outside of the
package. Special requirements such as refrigeration are clearly marked on the
outside of the carton and will be included in the unpacking instructions and
package inserts.
WARRANTY INFORMATION
All instruments manufactured by Sysmex® are warranted against defective materials or
workmanship for a period of one year commencing on the installation date at the
customer's required location.
This Warranty does not cover any defect, malfunction, or damage due to:
1. Accident, neglect or willful mistreatment of the product
2. Failure to use, operate, service, or maintain the product in accordance with the
applicable Sysmex Operator's Manual
3. Failure to use the appropriate reagents or chemicals specified for the product

Sysmex SF-3000 Operator's Manual -- Revised December 1995 i
SF-3000 OPERATOR'S MANUAL
TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION
1. INTRODUCTION....................................................................................1-1
2. OPERATOR'S MANUAL ORGANIZATION........................................1-2
3. INFORMATIONAL HEADINGS ...........................................................1-3
3.1 NOTE, CAUTION and WARNING ......................................1-3
3.2 Document Conventions ..........................................................1-3
4. SYSTEM OVERVIEW............................................................................1-4
5. SYSTEM OPTIONS ................................................................................1-5
6. SAFETY...................................................................................................1-6
6.1 General Safety ........................................................................1-6
6.2 Hazardous and Biohazardous Material...................................1-6
6.3 Reagent Safety........................................................................1-6
6.4 Laser Safety............................................................................1-7
7. OPERATIONAL SUMMARY ................................................................1-8
8. ANALYSIS PARAMETERS...................................................................1-9
9. DISPLAY SCREEN...............................................................................1-10
9.1 LCD (Liquid Crystal Display) Screen..................................1-10
9.2 Root Menus ..........................................................................1-14
9.3 Hard Copy ............................................................................1-16
10. PASSWORD ..........................................................................................1-17
11. EMERGENCY SHUTDOWN PROCEDURE ......................................1-18
12. ALARMS ...............................................................................................1-18
13. INSTALLATION REQUIREMENTS...................................................1-19
13.1 Relocation Information.........................................................1-19
13.2 Electrical Requirements .......................................................1-19
13.3 Space Requirements .............................................................1-20
13.4 Installation Environment ......................................................1-20
14. SYSTEM SPECIFICATIONS ...............................................................1-21
CHAPTER 2: SAMPLE PROCESSING
1. INTRODUCTION....................................................................................2-1
1.1 Summary of Operation Mode ................................................2-1
1.2 Operational Procedures in Each Mode ..................................2-2
2. START-UP PROCEDURE ......................................................................2-3
2.1 Operator Checks ....................................................................2-3
2.2 Turning On the Power ...........................................................2-4
2.3 Self-Checks............................................................................2-4
2.4 Pressure Gauge Check ...........................................................2-6
2.5 Auto Output Settings Check ..................................................2-6
3. QUALITY CONTROL ANALYSIS........................................................2-7
3.1 QC Analysis : Manual Mode .................................................2-7
3.2 QC Analysis : Auto Mode ...................................................2-12
3.3 QC Analysis : Manual Closed Mode...................................2-13
3.4 QC Analysis : Manual CP Mode .........................................2-17
(To be continued)

ii Sysmex SF-3000 Operator's Manual -- Revised September 1995
4. ANALYSIS MODE PROCEDURES....................................................2-18
4.1 Manual Mode ......................................................................2-18
4.2 Capillary Mode....................................................................2-23
4.3 Auto Mode...........................................................................2-29
4.4 Manual Closed Mode..........................................................2-37
4.5 Manual CP Mode.................................................................2-43
5. SHUTDOWN.........................................................................................2-49
5.1 Shutdown Procedure............................................................2-49
6. TIMER MODE ...................................................................................... 2-51
6.1 Restarting the Pneumatic Unit.............................................2-51
CHAPTER 3: WORK LIST
1. INTRODUCTION ...................................................................................3-1
1.1 Order Inquiry Method........................................................... 3-1
2. EXECUTING A WORK LIST PROGRAM ...........................................3-2
2.1 Work List Display.................................................................3-2
2.2 Data Entry.............................................................................3-3
3. DOWNLOAD LIST.................................................................................3-6
3.1 From Host Computer (HC)...................................................3-6
3.2 From Floppy Disk (FD)........................................................3-8
4. DELETE.................................................................................................3-10
4.1 All Data...............................................................................3-10
4.2 Marked Data........................................................................3-11
4.3 Current Data........................................................................3-12
5. GP OUTPUT..........................................................................................3-13
6. BACKUP ...............................................................................................3-14
CHAPTER 4: RESULT INTERPRETATION/REPORTS
1. INTRODUCTION ...................................................................................4-1
2. INTERPRETIVE (IP) MESSAGES........................................................4-2
3. LIST DISPLAY/STORED DATA...........................................................4-8
3.1 Sample Information Screen (Page 1)...................................4-10
3.2 CBC8 Screen (Page 2).........................................................4-13
3.3 WBC+5Diff# Screen (Page 3).............................................4-14
3.4 WBC+5Diff% Screen (Page 4)........................................... 4-15
3.5 RBC/PLT Screen (Page 5) ..................................................4-16
3.6 IP Messages Screen (Page 6)...............................................4-18
4. GRAPHIC DISPLAY............................................................................4-20
5. DATA VALIDATION...........................................................................4-24
6. MARK DATA........................................................................................4-25
7. SELECT DISPLAY...............................................................................4-26
7.1 Select By Flagging..............................................................4-27
7.2 Select By Date.....................................................................4-29
7.3 Select By No Output............................................................4-31
7.4 QC Data...............................................................................4-33
7.5 All Samples.........................................................................4-34
7.6 Read FD...............................................................................4-35
(To be continued)
Sysmex SF-3000 Operator's Manual -- Revised July 1996

Sysmex SF-3000 Operator's Manual -- Revised September 1995 iii
8. SEARCH STORED DATA....................................................................4-38
7.1 Top/Bottom..........................................................................4-38
7.2 By Sample No......................................................................4-39
7.3 Same Day.............................................................................4-40
7.4 By Date ................................................................................4-41
9. SORT STORED DATA .........................................................................4-43
9.1 Sequence Order....................................................................4-43
9.2 Sample No. Order ................................................................4-45
10. EDIT SAMPLE INFORMATION .........................................................4-46
10.1 Sample No. ..........................................................................4-47
10.2 ID Read Status .....................................................................4-48
10.3 POS to NEG.........................................................................4-48
11. OUTPUT STORED DATA....................................................................4-49
11.1 Current Data.........................................................................4-51
11.2 Marked Data ........................................................................4-52
11.3 All Data................................................................................4-52
11.4 Cancel ..................................................................................4-53
11.5 Backup (FD) ........................................................................4-54
12. DELETE.................................................................................................4-58
12.1 Current Data.........................................................................4-59
12.2 Marked Data ........................................................................4-60
12.3 All Data................................................................................4-61
13. AUTO OUTPUT ....................................................................................4-62
CHAPTER 5: MAINTENANCE AND SUPPLIES
1. INTRODUCTION ....................................................................................5-1
2. SF-3000 MAINTENANCE CHECK LIST ..............................................5-3
3. DAILY MAINTENANCE........................................................................5-4
3.1 Clean Detector Chamber and Manometer (Shutdown) .........5-4
3.2 Remove Fluid from Pneumatic Unit Trap Chamber..............5-6
4. WEEKLY MAINTENANCE ...................................................................5-7
4.1 Clean SRV Tray.....................................................................5-7
4.2 Execute Clean WBC Detector Sequence...............................5-9
5. MONTHLY MAINTENANCE..............................................................5-14
5.1 Clean Orifice........................................................................5-14
5.2 Execute Clean Waste Chamber Sequence ...........................5-16
5.3 Clean Sample Rotor Valve (SRV).......................................5-21
6. YEARLY MAINTENANCE..................................................................5-26
6.1 Visual Inspection for Laser Safety ......................................5-26
7. AS NEEDED MAINTENANCE............................................................5-29
7.1 Clean Manual Rinse Mechanism.........................................5-29
7.2 Execute RBC Aperture Clog Removal Sequence................5-31
7.3 Execute Clean RBC Transducer Sequence..........................5-33
7.4 Clean RBC Detector Aperture.............................................5-38
7.5 Execute Remove Flow Cell Air Bubbles Sequence ............5-41
7.6 Execute Replace Sheath Flow Reagent Sequence...............5-43
7.7 Replace Waste Container (if used) ......................................5-45
(To be continued)

iv Sysmex SF-3000 Operator's Manual -- Revised September 1995
8. REPLACEMENTS.................................................................................5-46
8.1 Replace Reagent ..................................................................5-46
8.2 Replace the Piercer (Auto Sampler Unit) ............................5-51
8.3 Replace the Piercer (Manual CP Unit) ................................5-58
8.4 Replace the Hand Clipper....................................................5-62
8.5 Replace the Rubber Plate No. 39.........................................5-63
8.6 Replace the Anti-Shock Rubber No. 49 ..............................5-64
8.7 Replace the Fuses ................................................................5-65
8.8 Replace the Belt No. 6.........................................................5-67
9. RESET THE COUNTER .......................................................................5-69
10. FORMAT FD..........................................................................................5-72
11. SUPPLIES AND REPLACEMENT PARTS LISTING.........................5-74
CHAPTER 6: QUALITY CONTROL
1. INTRODUCTION....................................................................................6-1
1.1 Types of QC Method.............................................................6-2
1.2 QC Parameters To Be Monitored..........................................6-2
2. DELETE FILE .........................................................................................6-3
3. SYSTEM SETTINGS ..............................................................................6-5
4. QC PARAMETER SELECTION ............................................................6-7
4.1 Selecting QC Parameter ( X /L-J Control) ...........................6-7
4.2 Selecting QC Parameter ( X M Control) ..............................6-8
5. SETTING X M CONTROL .................................................................6-10
5.1 Executing the X M Control Settings Program...................6-10
6. TARGET/LIMIT....................................................................................6-13
6.1 Display.................................................................................6-13
6.2 Read FD (Floppy Disk) .......................................................6-15
6.3 Help .....................................................................................6-19
6.4 Auto Setting.........................................................................6-21
6.5 Manual Change....................................................................6-25
6.6 Lot Information ...................................................................6-28
7. QC CHART............................................................................................6-31
7.1 Select File............................................................................6-33
7.2 Output QC Chart/Data.........................................................6-34
7.3 Mean/SD (Calculation)........................................................6-36
7.4 Delete Plot ...........................................................................6-38
7.5 Cal. History .........................................................................6-40
8. MANUAL QC ANALYSIS ...................................................................6-41
9. SAMPLER QC ANALYSIS (L-J) .........................................................6-44
10. QC METHODS AND APPLICATIONS...............................................6-47
10.1 X Control ..........................................................................6-47
10.2 L-J Control ..........................................................................6-47
10.3 X M Control.......................................................................6-48
11. VARIABLE TARGET FUNCTION......................................................6-51
11.1 How Target Values Are Calculated.....................................6-51
11.2 Variable Target Function and Erroneous Data....................6-52
12. CONTROL CHART DEFINITION.......................................................6-53

Sysmex SF-3000 Operator's Manual -- Revised September 1995 v
CHAPTER 7: CALIBRATION
1. INTRODUCTION....................................................................................7-1
1.1 Calibration Implementation Standards ..................................7-1
1.2 Calibration Samples...............................................................7-2
1.3 Reference Values...................................................................7-2
2. HGB/HCT AUTO ....................................................................................7-3
2.1 Overview of the HGB/HCT Auto Program...........................7-3
2.2 Setting (Entering Reference Values) .....................................7-6
2.3 Analyze (Analyzing)..............................................................7-8
2.4 Exclude (Excluding and Recovering Data) .........................7-10
2.5 Exe. Cal. (Calibrating).........................................................7-12
2.6 Graphic (Displaying Graphs)...............................................7-14
3. HGB/HCT MANUAL............................................................................7-15
3.1 Calculating the Compensation Factor..................................7-15
3.2 Executing the HGB/HCT Manual Program.........................7-16
4. CALIBRATION HISTORY...................................................................7-18
4.1 Displaying the Cal. History .................................................7-18
4.2 Deleting the Cal. History .....................................................7-19
4.3 Outputting the Cal. History..................................................7-20
CHAPTER 8: TROUBLESHOOTING
1. INTRODUCTION....................................................................................8-1
2. TROUBLESHOOTING BY ERROR MESSAGES ................................8-2
2.1 Alphabetical Error Message Index ........................................8-2
2.2 Contents (Error Messages By Function)................................8-5
2.3 Troubleshooting Guide ..........................................................8-9
3. TEST PROGRAMS ...............................................................................8-59
3.1 Executing a Test Program....................................................8-59
3.2 Test SRV..............................................................................8-62
3.3 Test Motor ...........................................................................8-63
3.4 Test PDA PCB.....................................................................8-69
3.5 Test Laser Unit ....................................................................8-70
3.6 Test Sampler ........................................................................8-71
3.7 Test Bar Code Reader..........................................................8-74
3.8 Test Memory........................................................................8-75
3.9 Test Floppy Disk..................................................................8-76
3.10 Test LCD Display................................................................8-78
3.11 Test Touch Panel .................................................................8-79
3.12 Test Output ..........................................................................8-80
3.13 Version Check .....................................................................8-83
3.14 Service Data.........................................................................8-85
4. STATUS DISPLAY...............................................................................8-90
5. ERROR LOG .........................................................................................8-94

vi Sysmex SF-3000 Operator's Manual -- Revised September 1995
CHAPTER 9: ADJUSTMENT
1. INTRODUCTION....................................................................................9-1
2. PRESSURE/VACUUM DISPLAY .........................................................9-1
2.1 Pneumatic Gauges ..................................................................9-1
2.2 Displaying the Monitored Values on the LCD.......................9-2
3. PNEUMATIC UNIT ADJUSTMENT.....................................................9-3
3.1 Adjusting the 2.0 kg/cm2........................................................9-4
3.2 Adjusting the Vacuum............................................................9-5
4. MAIN UNIT ADJUSTMENT..................................................................9-5
4.1 Adjusting to 0.6 kg/cm2.........................................................9-6
4.2 Adjusting to 0.5 kg/cm2.........................................................9-7
4.3 Adjusting the 250 mmHg Vacuum.........................................9-8
5. LCD CONTRAST ADJUSTMENT.........................................................9-9
6. ID BAR CODE READER ADJUSTMENT (Option)............................9-10
CHAPTER 10: FUNCTIONAL DESCRIPTION
1. INTRODUCTION..................................................................................10-1
2. DETECTION PRINCIPLES ..................................................................10-2
2.1 Flow Cytometry by Semiconductor Laser...........................10-2
2.2 DC Detection Method..........................................................10-3
2.3 SLS-Hemoglobin .................................................................10-3
3. SAMPLE FLOW BLOCK DIAGRAM .................................................10-4
4. RBC/PLT/HGB ANALYSIS .................................................................10-5
4.1 RBC/PLT Analysis Flow.....................................................10-5
4.2 HGB Analysis Flow.............................................................10-6
4.3 Red Blood Cell (RBC) Indices ............................................10-7
5. WBC CLASSIFICATION .....................................................................10-8
5.1 4DIFF Analysis....................................................................10-8
5.2 WBC/BASO Analysis .........................................................10-9
6. WBC SCATTERGRAMS....................................................................10-10
7. RBC/PLT PARTICLE SIZE DISTRIBUTION ANALYSIS ..............10-12
7.1 RBC Particle Size Distribution..........................................10-12
7.2 PLT Particle Size Distribution...........................................10-13
7.3 Particle Size Distribution Representation..........................10-14
7.4 Histogram Flags.................................................................10-15
8. MAIN UNIT ELECTRONIC SYSTEM ..............................................10-16
9. MAIN UNIT COMPONENTS.............................................................10-18
9.1 Front...................................................................................10-18
9.2 Rear....................................................................................10-20
9.3 Right Side ..........................................................................10-22
9.4 Left Side.............................................................................10-24
9.5 Front Interior......................................................................10-25
9.6 Left Interior........................................................................10-26
9.7 Right Interior .....................................................................10-30
(To be continued)

Sysmex SF-3000 Operator's Manual -- Revised March 1996 vii
10. PNEUMATIC UNIT COMPONENTS................................................10-32
10.1 Front...................................................................................10-32
10.2 Right Side ..........................................................................10-33
10.3 Top Interior........................................................................10-34
10.4 Right Interior .....................................................................10-34
11. CAP PIERCING SAMPLER UNIT (OPTION) ..................................10-35
11.1 Top.....................................................................................10-35
11.2 Top Interior........................................................................10-36
12. MANUAL CP UNIT (OPTION)..........................................................10-37
13. REAGENT SYSTEM...........................................................................10-38
CHAPTER 11: SYSTEM SETUP
1. INTRODUCTION..................................................................................11-1
2. AUTO MANAGEMENT PROGRAMS................................................11-3
2.1 Auto Output Condition ........................................................11-6
2.2 Auto Output Mode (Data Specification)..............................11-8
2.3 Auto Erase ...........................................................................11-9
2.4 Auto Validation .................................................................11-11
3. DATA CRITERIA ...............................................................................11-13
3.1 Mark Limits .......................................................................11-16
3.2 Critical Limits....................................................................11-18
3.3 Sampler Stop Limits ..........................................................11-20
3.4 Sampler Stop Condition.....................................................11-22
4. FLAG SYSTEM...................................................................................11-24
4.1 WBC ..................................................................................11-27
4.2 RBC ...................................................................................11-29
4.3 PLT ....................................................................................11-30
5. PERIPHERAL CONFIGURATION....................................................11-32
5.1 HC Setting (Host Computer) .............................................11-35
5.2 GP Setting (Graphic Printer) .............................................11-37
5.3 DP Setting (Data Printer)...................................................11-38
6. GENERAL SET UP .............................................................................11-41
6.1 Date/Time ..........................................................................11-44
6.2 Units...................................................................................11-45
6.3 Password............................................................................11-47
6.4 Timer Mode .......................................................................11-48
6.5 Normal Range Display ......................................................11-49
6.6 Date Format .......................................................................11-51
6.7 ID Reader...........................................................................11-52
6.8 Blood Sensor......................................................................11-54
6.9 Work List...........................................................................11-56
7. OUTPUT SET VALUES .....................................................................11-57
7.1 Output to GP.......................................................................11-57
7.2 Backup to FD......................................................................11-57

viii Sysmex SF-3000 Operator's Manual -- Revised December 1995
APPENDIX A: MENU TREE
APPENDIX B: INSTALLATION
1. PREINSTALLATION.............................................................................B-2
1.1 Unpacking Check List ..........................................................B-2
1.2 Supplementary Parts Information.........................................B-5
2. LAYOUT AND WORK SPACE ............................................................B-6
3. REMOVING PACKING MATERIAL...................................................B-7
3.1 Front Cover...........................................................................B-7
3.2 Front Panel............................................................................B-7
3.3 Main Unit Chassis.................................................................B-9
3.4 SRV.....................................................................................B-10
3.5 Rear Panel...........................................................................B-10
3.6 Pneumatic Unit ...................................................................B-11
4. TRAY ....................................................................................................B-11
5. BAR CODE ID READER (Optional)...................................................B-12
6. CP SAMPLER UNIT (Optional) ..........................................................B-13
6.1 Main Unit............................................................................B-13
6.2 Sampler Unit.......................................................................B-14
6.3 Connection to Main Unit ....................................................B-15
7. MANUAL CP UNIT (Optional) ...........................................................B-17
7.1 Main Unit............................................................................B-17
7.2 Manual CP Unit ..................................................................B-18
7.3 Connection to Main Unit ....................................................B-18
8. TUBE CONNECTION..........................................................................B-22
8.1 Vacuum Line ......................................................................B-22
8.2 Pressure Line ......................................................................B-22
8.3 Reagent Container Preparation...........................................B-23
8.4 Reagent Lines .....................................................................B-26
8.5 Waste Container..................................................................B-27
9. BATTERY BACKUP ...........................................................................B-28
10. CABLE AND POWER CORD CONNECTIONS................................B-29
10.1 Output Connections ............................................................B-29
10.2 Pneumatic Cable Connections ............................................B-30
10.3 Power Cord Connections ....................................................B-30
11. POWER ON AND SYSTEM SETUP...................................................B-31
11.1 Power On ............................................................................B-31
11.2 System Setup ......................................................................B-31
11.3 System Check .....................................................................B-32

Sysmex SF-3000 Operator's Manual -- Revised September1995 ix
APPENDIX C: TECHNICAL INFORMATION
1. SERIAL INTERFACE FOR HOST COMPUTER.................................C-2
1.1 Connection............................................................................C-2
1.2 Input/Output Signals.............................................................C-2
1.3 Communication Format........................................................C-2
1.4 Baud Rate/Character Structure .............................................C-2
1.5 Signal Level..........................................................................C-3
1.6 Interface Circuit....................................................................C-3
1.7 Software................................................................................C-4
2. TEXT FORMAT .....................................................................................C-8
2.1 Sample Data Format .............................................................C-8
2.2 Quality Control Data Format..............................................C-12
2.3 Inquiry Data Format ...........................................................C-14
2.4 Order Information Data Format..........................................C-15
3. ID BAR CODE......................................................................................C-17
3.1 Applicable Bar Codes.........................................................C-17
3.2 Dimensions of Elements.....................................................C-18
3.3 Requirements on Wide/Narrow Ratio ................................C-18
3.4 Optical Requirements .........................................................C-18
3.5 Dimensions of Bar Code Label...........................................C-20
3.6 Check-Digit.........................................................................C-20
3.7 Applicable Characters.........................................................C-28
3.8 Effective Bar Code Length .................................................C-28

Revised September 1995
CHAPTER 1 INTRODUCTION
1. INTRODUCTION ...............................................................1-1
2. OPERATOR'S MANUAL ORGANIZATION...............................1-2
3. INFORMATIONAL HEADINGS .............................................1-3
3.1 NOTE, CAUTION and WARNING....................................1-3
3.2 Document Conventions...................................................1-3
4. SYSTEM OVERVIEW ..........................................................1-4
5. SYSTEM OPTIONS.............................................................1-5
6. SAFETY ........................................................................1-6
6.1 General Safety .............................................................1-6
6.2 Hazardous and Biohazardous Material..................................1-6
6.3 Reagent Safety.............................................................1-6
6.4 Laser Safety ................................................................1-7
7. OPERATIONAL SUMMARY..................................................1-8
8. ANALYSIS PARAMETERS ...................................................1-9
9. DISPLAY SCREEN...........................................................1-10
9.1 LCD (Liquid Crystal Display) Screen................................. 1-10
9.2 Root Menus ..............................................................1-14
9.3 Hard Copy................................................................1-16
10. PASSWORD....................................................................1-17
11. EMERGENCY SHUTDOWN PROCEDURE .............................1-18
12. ALARMS........................................................................1-18
13. INSTALLATION REQUIREMENTS.......................................1-19
13.1 Relocation Information..................................................1-19
13.2 Electrical Requirements.................................................1-19
13.3 Space Requirements.....................................................1-20
13.4 Installation Environment................................................1-20
14. SYSTEM SPECIFICATIONS................................................1-21

INTRODUCTION
Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-1
1. INTRODUCTION
The Sysmex® SF-3000 is a technically advanced, computerized, fully automated
hematology analyzer with many features, for in vitro diagnostic use in clinical
laboratories. The SF-3000 provides accurate screening for patients whose hematological
abnormalities indicate the need for further testing. In addition, it can contribute in the
diagnosis and therapeutic monitoring of patients.
The SF-3000 has the capability of analyzing up to 80 samples per hour, and display 4
different patterns -- WBC 5 differential, scattergrams for white blood cells and basophils,
and cell size distribution curves for red blood cells and platelets, as well as 23 analysis
data parameters on the color LCD (liquid crystal display) screen.
Chapter 1 is an introduction to this manual, major system components, system operation
and other general considerations. This chapter should be read before operating the
SF-3000. Chapter 1 contains the following subjects:
Manual Organization
A description of the Operator's Manual and explanation of important informational
headings.
System Overview
An introduction to the main elements of the SF-3000 analyzer, including a description of
the options available to enhance the instrument's operation.
Safety Summary
Covers major safety considerations when using and servicing the SF-3000.
Operational Summary
A summary of the operating procedures for each mode, the LCD screen display,
functions of touch panel, etc.
Installation Requirements
Includes space requirements, necessary equipment, installation environment and other
essential information to be reviewed before installation of the SF-3000.
Instrument Specifications
The instrument's specifications are given in a reference table.

INTRODUCTION
1-2 Sysmex SF-3000 Operator's Manual -- Revised September 1995
2. OPERATOR'S MANUAL ORGANIZATION
The SF-3000 Operator's Manual has been designed with the operator in mind. Each
chapter has its own table of contents and is designed to facilitate rapid location of
appropriate information.
Table 1-1: Manual Organization
CHAPTER 1
Introduction Contains basic information about the instrument; operating procedures, screen displays, touch panel, installation and safety. This chapter must be
read prior to operating the Sysmex SF-3000.
CHAPTER 2
Sample Processing Explains sampling procedure from start-up to shutdown. All sampling operation procedures are contained in this chapter.
CHAPTER 3
Work List Explains how to enter order information with the display and management of the records.
CHAPTER 4
Result Interpretation/
Reports
Explains procedures for displaying and processing Stored Data, and
procedures for analyzed result output. Also explains IP messages.
CHAPTER 5
Maintenance and Supplies Explains periodic maintenance, and the procedures for replacing consumables and reagents.
CHAPTER 6
Quality Control Explains how to run the Quality Control Program. Also explains, for your reference, the quality control system used in this instrument.
CHAPTER 7
Calibration Explains the procedures for auto-calibration and manual calibration.
CHAPTER 8
Troubleshooting Explains error messages and their troubleshooting. Also explains service data and system test functions.
CHAPTER 9
Adjustments Explains how to adjust pressure and other adjustments.
CHAPTER 10
Functional Descriptions Gives the main principles of analysis performed by the instrument. Also contains system nomenclature.
CHAPTER 11
System Setup Explains how to set date, time, units, sample analysis stop and other system conditions. Also contains data analysis settings -- for abnormal data
judgments, etc.
APPENDICES A, B, C
Menu Tree, Installation
and Technical Information
Contains program menu tree, technical information including unpacking check
lists, installation procedures, bar-code labeling parameters and host
communication protocols.

INTRODUCTION
Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-3
3. INFORMATIONAL HEADINGS
3.1 NOTE, CAUTION and WARNING
NOTE, CAUTION and WARNING boxes throughout this manual are meant to get to
attention to important safety and operational information. Always follow the instructions
contained in the boxes. Failure to do so may affect the safety or precision features of the
SF-3000 analyzer. The boxes are defined as follows:
NOTE: Highlights important facts, gives helpful information, and clarifies
procedures.
CAUTION: Provides information on the correct operation of the SF-3000 analyzer.
Information contained in CAUTION boxes is crucial to prevent damage
and to maintain performance of the analyzer.
WARNING! Indicates potentially hazardous situations that could result in serious
injury or infection to laboratory personnel.
3.2 Document Conventions
The SF-3000 Operator's Manual uses the following conventions:
"Quotations": Screen commands, messages, and status information that the SF-
3000 system displays appear within quotation marks. For example:
"All Samples", "Ready" or "Input Password"
[Square Brackets]: Names of function keys such as control or operational keys,
number keys, or arrow keys that you select on the SF-3000 appear within square
brackets. For example:
[Enter], [Sampler], [2] or [↑]
Italics: Application names and reference material appear in italic form. For
example:
Refer to Chapter 2: Sample Processing.

INTRODUCTION
1-4 Sysmex SF-3000 Operator's Manual -- Revised September 1995
4. SYSTEM OVERVIEW
The purpose of the SF-3000 is to provide accurate results on 23 parameters and to detect
abnormalities in the sample. To assist the laboratory in the screening of abnormalities,
the SF-3000 analyzes and processes the data obtained from the analyzer unit, and the
LCD displays and outputs either a POSITIVE or a NEGATIVE message for each sample.
A NEGATIVE message signifies that parameter values for the sample are within
acceptable limits and the sample does not require any additional verification of those
values. A POSITIVE message signifies that the sample is not within the acceptable limits
and requires further review and investigation. Proper use of the instrument requires each
laboratory to establish the acceptable limits for results.
The SF-3000 uses the optical detector block incorporating the semiconductor laser to
analyze the WBC population. In the DIFF channel, lymphocytes, monocytes,
eosinophils, and the group of neutrophils and basophils are analyzed to determine the
WBC 4-differentials using flow cytometry. Basophil determination and WBC number
counting are performed in the BASO channel. RBCs and PLTs are analyzed by the RBC
detector block using the DC detection method. HGB determination is performed using
the SLS hemoglobin detection method.
The SF-3000 consists of two major systems.
• Main Unit : Houses the hydraulic and electronic systems and their control
components. The unit analyzes, processes and displays the data
on the LCD screen.
• Pneumatic Unit : Supplies the required pressure and vacuum for analysis.
Main Unit
Pneumatic Unit
Sampler Unit
(Optional)
Figure 1-1: Sysmex SF-3000 Hematology Analyzer

INTRODUCTION
Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-5
5. SYSTEM OPTIONS
Several options are available to enhance system efficiency. Available options for the
SF-3000 are:
• ID Bar Code Reader : Reads the ID numbers of bar code labeled samples,
and inputs the numbers automatically.
• Color Graphics Printer : Prints out analysis results (cell size distribution
or Page Printer graphs, scattergrams) and hard copies of screens in
color or black and white.
Printing can be done both in graphic and list format.
• Data Printer : Prints the analysis data in ticket format.
• (Cap Piercing) Sampler Unit : Consists of a mixer and a cap piercer unit, and loads
up to 5 sample racks (50 sample tubes) that enable an
automated closed sampling system.
• Manual CP Unit : Consists of a mixer and a cap piercer unit which
enables a manual closed sampling system.

INTRODUCTION
1-6 Sysmex SF-3000 Operator's Manual -- Revised September 1995
6. SAFETY
The operator must follow the instructions in this section. It contains important
information to operate the SF-3000 analyzer safely.
6.1 General Safety
While operating, maintaining, servicing or repairing the system, follow all procedures
contained in this manual. The operator must observe all NOTES, CAUTIONS and
WARNINGS posted anywhere on the instrument or given in this manual.
Do NOT touch the electrical circuits behind the enclosure panels of the SF-3000.
Touching the circuits is dangerous and may give you an electrical shock, especially if
your hands are wet.
Use only designated tools and parts for service and repair. Substitute parts should never
be used, and the SF-3000 should not be modified in any way.
Keep hair, clothing and fingers away from all moving parts.
Install the Sampler Unit on a table or work bench to prevent the system from accidentally
falling.
6.2 Hazardous and Biohazardous Material
All surfaces and components in contact or potentially in contact with patient blood
samples should be considered contaminated. Use of protective garments and gloves is
strongly recommended when operating, maintaining, or servicing the SF-3000.
Do not touch the waste when disposing of it or assembling/disassembling the associated
parts. If accidental contact with waste or any other potentially biohazardous material
occurs, flush the affected area immediately with water and follow your laboratory's
prescribed cleaning and decontamination procedures.
Consider all regulations applicable to your laboratory when operating the Sysmex
SF-3000 instrument.
6.3 Reagent Safety
If reagent comes into contact with skin or clothing, flush it with water. If eyes are
affected, flush with copious amounts of water and obtain medical advice if necessary.

INTRODUCTION, Safety
Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-7
6.4 Laser Safety
The SF-3000 uses a semiconductor laser unit. Though this laser unit is placed in the
shield box, the operator must observe all NOTES, CAUTIONS and WARNINGS
concerning laser handling.
WARNING!: DO NOT remove the shield box cover of the Laser Unit.
Semiconductor Laser
Unit Cover
< Top View >
< Left Side View >
NOTE: This caution label is located
inside the Laser Unit Cover.
Figure 1-2: Location of Laser Unit and Labels
(Left Interior of the Main Unit)
Refer to Chapter 5, Section 6 for further information on laser handling.