Thermo IEC Centra-GP8 User manual

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OPERATION
MANUAL
OM3121
Revision 7
Centra-GP8
Ventilated Centrifuge
Cat. No. 3121 -- For 100/120/220/240 VAC, 50/60 Hz
Centra-GP8R
Refrigerated Centrifuge
Cat. No. 3122 -- For 120 VAC, 60 Hz
Cat. No. 3125 -- For 220/240 VAC, 50/60 Hz
Centra-GP8(F)
Ventilated Floor Model Centrifuge
Cat. No. 3127 -- For 100/120/220/240 VAC, 50/60 Hz
Centra-GP8R(F)
Refrigerated Floor Model Centrifuge
Cat. No. 3128 -- For 120 VAC, 60 Hz
Cat. No. 3129 -- For 220/240 VAC, 50/60 Hz
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Centra-GP8/GP8R Operation Manual 1
TABLE OF CONTENTS
1INTRODUCTION .................................................................................................2
2INSTALLATION ...................................................................................................3
2.1 ReceivingtheUnit......................................................................................3
2.2 SitePreparation .........................................................................................3
ClearanceEnvelope ..................................................................................3
2.3 PowerConfiguration ..................................................................................3
TableForElectric Configuration................................................................4
Voltage .......................................................................................................5
Fuses .........................................................................................................5
PowerCord ................................................................................................6
2.4 Moving the Unit ..........................................................................................6
3OPERATION....................................................................................................... 7
3.1 The Front Panel ......................................................................................... 7
3.2 RotorandAccessories .............................................................................. 10
Balance ......................................................................................................10
Vibration .....................................................................................................11
RotorInstallation/Removal ........................................................................ 11
AddingRotors ............................................................................................11
DeletingRotors .......................................................................................... 12
3.3 Starting And Stopping A Run ....................................................................12
3.4 OperatingModes ....................................................................................... 13
ManualOperation ...................................................................................... 13
RapidCondition(GP8R Only) ...................................................................13
HoldMode.................................................................................................. 14
3.5 StoredPrograms........................................................................................ 14
Locking/Recall/Add/Change ......................................................................14
3.6 Refrigeration(GP8ROnly) ........................................................................ 15
3.7 Fail-SafeSystems ..................................................................................... 16
4ACCESSORIES................................................................................................... 17
4.1 SpeedandForceTables ...........................................................................17
4.2 Derating Table for Dense Samples ...........................................................23
4.3 ChemicalResistanceTable....................................................................... 24
4.4 DecontaminationTable..............................................................................25
4.5 Nomograph ................................................................................................26
5MAINTENANCE...................................................................................................27
5.1 Cleaning .....................................................................................................27
5.2 CoverInterlock Bypass .............................................................................29
5.3 Calibration.................................................................................................. 29
5.4 BrushReplacement ................................................................................... 29
5.5 Warranty ....................................................................................................30
5.6 Conditionof ReturnedEquipment .............................................................31
5.7 Table of Spare Parts..................................................................................31
5.8 Fuses Not Replaceable By The Operator.................................................32
6SPECIFICATIONS............................................................................................... 33
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Centra-GP8/GP8R Operation Manual
2
1 INTRODUCTION
TheCentra-GP8seriesaregeneral purpose centrifuges designed foruse
in medical, industrial and scientific laboratories. The Centra-GP8 series is
available in the following models.
Benchtopmodels: 3121 - GP8 100/120/220/240Vac,50/60 Hz
3122 - GP8R 120 Vac, 60 Hz
3125 - GP8R 200/220/240Vac,50/60Hz
Kneewellmodels: 3123 - GP8(K) 100/120/220/240Vac,50/60 Hz
3124 - GP8R(K)120 Vac, 60 Hz
3126 - GP8R(K) 200/220/240 Vac, 50/60 Hz
FloorModels: 3127 - GP8(F) 100/120/220/240Vac,50/60 Hz
3128 - GP8R(F)120 Vac, 60 Hz
3129 - GP8R(F)220/240 Vac, 50/60 Hz
The Centra-GP8 series can develop a maximum relative centrifugal force
(RCF) of 4630 xg using the 822A rotor. The centrifuges will accommodate
a range of centrifuge tubes and devices including 750 mL bottles,
microplates, cytological slide carriers, and microsample tubes. Maximum
sample load is 3 Liters.
Designed for ease of use, the Centra-GP8 has an ergonomic touch pad
control panel and bright, easily read LED displays. The unit can be
operated in manual mode, hold, or one of 35 programmable operations.
Programs can also be modified at run time, offering unlimited run
variations. In addition, rotor number entry permits automatic calculation of
RCF. Other features include a coast mode and separate acceleration and
deceleration controls for maintaining delicate samples such as those with
densitygradients.
All refrigerated models allow you to select chamber temperatures from
-5°C to 40°C and will maintain 2°C at full speed with the 218 rotor. All
refrigerated models also offer a Rapid Condition function for pre-cooling
or pre-heating the rotor and sample chamber. Repeat runs with precisely
the same temperature, speed and time setting can be achieved with the
touch of a button.
The internal microprocessor that allows this simplified operation also
ensuresrepeatableresults,preventinginadvertent loss of sample,and
evenalertsoperatorswhen periodic maintenance isdue.
The Centra-GP8 series rugged steel cabinet and rigid construction
providequietoperation andlong-termreliabilitycoupled withimpressive
safety features. A fail-safe cover interlock prevents the rotor from running
unless the cover is closed. It also prevents the cover from being opened
until the rotor has slowed to less than 90 RPM, even if the power fails. If a
load-imbalance occurs, a sensor shuts the unit down and triggers a
warningmessage.
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Centra-GP8/GP8R Operation Manual 3
2 INSTALLATION
IEC ships the centrifuge in a carton that protects it from shipping hazards.
Follow the unpacking instructions on the carton. Be sure to complete the
postage-paid warranty card and return it to IEC (U.S. and Canada) or to
thelocaldistributor(Export).
2.2 Site Preparation
For benchtop units, place the unit on a smooth, clean, dry surface to
ensure that the suction feet grip firmly. The surface must be rigid, stable
andleveltoensurequiet, vibration-free operation.
Clear the area beneath the unit of debris and loose material such as
paper. Allow 8 cm (3 inches) of clearance near the ventilation grill of
refrigeratedunits.
For Kneewell and Floor Model units, make sure that the floor is clean,
stable and level, and that the unit has at least 8 cm (3 inches) of
clearance for the GP8 and 16 cm (6 inches) of clearance for the GP8R at
the rear for ventilation.
Warning: Lock the front wheels before starting a run to avoid
dangerous movement. To gain access to the caster locks, pull the
centrifuge forward about 15 cm (6 inches) and then swing backwards to
swivel the casters to the front.
Section 6 of this manual provides specific dimensions and specifications
for each of the Centra-GP8 units.
Clearance Envelope InternationalElectrotechnicalCommission standard1010part 2-20limits
the permitted movement of a laboratory centrifuge to 300mm in the event
of a disruption. The user should therefore mark the clearance envelope
boundaryaround thecentrifuge,orlaboratorymanagementprocedures
should require that no person or any hazardous materials are within such
a boundary while the centrifuge is operating.
2.3 Power Configuration
Do not plug in the centrifuge until you have configured the power
correctly. For best results, the centrifuges should be used on a
dedicated line. Variations in line voltage or frequency will affect the unit’s
speed and other characteristics. Less than nominal line voltage may
preventthecentrifugefromreaching published specifications ofspeed
and/or temperature. Also, power line voltage at some locations may sag
when the refrigeration system turns on.
Caution: Configuring the centrifuge incorrectly may damage the
equipment and will void your warranty.
2.1 Receiving the Unit
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Centra-GP8/GP8R Operation Manual
4
Table for Electric Configuration
3121, 3123, 3127
100/120/220/240 120Voltage (AC) 200/220/240
50/60 60Frequency (Hz) 50/60
3122, 3124, 3128Model No. 3125, 3126, 3129
Fuse Requirement
(all fuses are 250 V,
Slo-Blo Type T
glass 5 x 20 mm)
10 A 15 A 10 A
Power Line
Ampacity
Note: Power line ampacity requirement provides for sufficient current to permit
effective performance.
200
220
240 V
two
16A at {
one
20A at 120 V
Voltage Range 108-132 V
90-110 V; set 100 V
108-132 V; set 120 V
198-242 V; set 220 V
216-264 V; set 240 V
Centra-GP8 &
GP8(K/F) Centra-GP8R & GP8R(K/F) Centra-GP8R & GP8R(K/F)
180-220 V; set 200 V
198-242 V; set 220 V
216-264 V; set 240 V
two
16A at 100 120 V
220
240 V
two
6.3A at
{
{
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Centra-GP8/GP8R Operation Manual 5
Voltage Use a volt meter to measure the voltage at your site. For models 3121,
3123, 3125, 3126, 3127 and 3129: Locate the power entry module on the
lower left side of the unit. On the right side of the module is the fuse
drawer. A small latch on the left holds this drawer in place. Press the
latch and slide the drawer out. If the number visible in the window differs
from the voltage at your site, remove the square insert, rotate it, and
reinstall it so that the correct voltage is displayed through the window.
Fuses Install appropriate fuses for the voltage at your site.
Centra-GP8 & Centra-GP8(K) (model #3121, #3123, #3127):
two 16A fuses for 100/120 V; or two 6.3A fuses for 220/240 V.
Centra-GP8R&Centra-GP8R(K)(model#3122,#3124,#3128):
Thesemodelshavenoinsertorwindowandarealreadyconfiguredfor
use with 120V, 60 Hz only.
Centra-GP8R & Centra-GP8R(K) (model #3125, #3126, #3129):
two 16A fuses.
Ensuring that the fuses are securely in place, reinstall the entire drawer in
the side of the centrifuge.
Fuse Drawer
Remove and
Rotate for
Proper Voltage
Fuse
240
Power Entry Module
Latch
Fuse
Drawer
Voltage
Selection
Window
POWER ENTRY MODULE FUSEDRAWER
Circuit Breaker Centrifuges are fitted with either a single pushbutton circuit breaker or a
dualpushbuttonswitch/circuitbreaker. Thedual pushbutton switch/circuit
breaker may be used as an On/Off switch for the centfuge as follows:
The circuit breakers are located in the base of the centrifuge. The dual
pushbutton swicth/circuit breaker is identified by the red and green
buttons. Pressing the green button connects power and resets the
breaker. Power may be disconnected by pressing the red button or by
unplugging the power cord from the centrifuge.
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Centra-GP8/GP8R Operation Manual
6
Power cord TheCentra-GP8Rrequires agroundedpower supply(3-prongpower
outlet). If your facility does not have properly grounded outlets, arrange
forpropergrounding.
IEC provides two power cords with each Centra-GP8 and Centra-GP8R.
One is suitable for North America, Japan and Korea. The other has bare
wires at one end so other plug types can be attached.
Caution: Do not remove the grounding pin from the centrifuge
power cord. Do not use the bare wire power cord to attach a power
plug that does not have a grounding pin. Use only the appropriate
power cord supplied by IEC.
If the bare wire power cord is used, install the selected plug and attach
the cord to the receptacle on the lower left side of the centrifuge. Plug into
the power outlet.
Warning: The power cord(s) provided with the unit is correctly rated
for the highest current demand. This power cord should not be
interchanged with cords from equipment with lower current
demand. Exchange of power cords between equipment may create
a fire hazard.
2.4 Moving the Unit
If you relocate a GP8 series centrifuge to a different power source, please
refer to section 2.3 to check the power requirements and, if necessary,
reconfigurethepower.
Benchtop First, remove the rotor and accessories. Next, release the seal
on the suction cups that adhere the Centra-GP8/R to the work surface.
Lift the edge of each suction cup to release the seal and insert an object
such as a tongue depressor underneath to prevent the cup from
resealing. Position the device in its new location and check the cups to
ensuretheyaregrippingthe benchtop properly.
Kneewell To move the kneewell centrifuge, remove the rotor and accessories.
Unlock the front casters and grasp the centrifuge by its side handles and
wheel into position. Maneuver it backward to expose the locking casters
again. Lock down the casters.
Warning: Lock the front wheels before starting a run to avoid
dangerousmovement.
Floor Model To move the floor model centrifuge, remove the rotor and accessories.
Unlock the front casters and grasp the centrifuge by the corners of the
cabinet and wheel into position. Maneuver it backward to expose the
locking casters again. Lock down the casters.
Warning: Lock the front wheels before starting a run to avoid
dangerousmovement.
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Centra-GP8/GP8R Operation Manual 7
3 OPERATION
3.1 The Front Panel
FRONT PANEL OF THE CENTRA-GP8R
Run parameters are selected using the touch switches on the
control panel. An audible beep signals when a switch has been
pressed. The arrow keys are used to select the different parameters.
The Digital Display indicates both the actual and set parameters for rotor
speed or g-force, run time, chamber temperature, program number and
rotor number or rotor radius. The display normally operates at full
brightnesswhenindicatingactual run conditions.However,the display
dims when indicating set conditions, or while the arrow keys are being
usedtochange parameters.
The On/Off button turns on the power to the display and to the
refrigeration system (GP8R). The unit must be on to open the lid, or
operatethecontrols.
Arrow keys are used to view or change the desired settings for
temperature, RPM/RCF, time or rotor/radius. Pressing an arrow once
changes the display momentarily from actual readings to the program
settings. Pressing the arrow twice allows you to re-program the run
parameters. To raise or lower a setting by one increment, press and
release the appropriate arrow key. To adjust in greater increments, hold
the arrow key down and the settings will change slowly at first and then
accelerate. As you approach the proper setting, release the button and
then press it repeatedly to select the exact setting. When a button is
released for three seconds, the display returns to actual readings.
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Centra-GP8/GP8R Operation Manual
8
The number under this symbol represents temperature in whole degrees
Celsius. This can be set between -5 and 40°C in 1° increments. The
display range is -9 to 45° C, and the accuracy is ±1°C from 2°C to
ambient. If the actual temperature is not within 5° of the set temperature
when the run button is pressed, the run will start, a beep will sound for 1.5
seconds to alert the user, and the temperature display will alternate
between the set point and the actual temperature until the chamber
temperature is within 5° of the setting.
The display under this symbol indicates either the rotor speed (RPM), or
the Relative Centrifugal Force (RCF). Speeds are shown in increments of
10 rpm. RCF is shown in 1xg increments below 100xg and 10xg
increments above 100xg. Speed can be set between 500 and 6000 rpm
in 10 rpm increments. RCF can be set in 1xg increments up to 100xg,
and 10xg increments from 100xg to 4630xg. When entering speed, the
last digit is fixed at zero and cannot be changed. The accuracy of the
speed control is ±10 rpm.
This button toggles between RPM and RCF. When RPM is selected, the
speed indicator displays revolutions per minute. When RCF is selected,
the speed indicator displays relative centrifugal force, and the rotor/radius
indicator displays either the rotor number or the rotor radius. RCF is only
indicated when a rotor number has been selected in the rotor/radius
display.
Time can be set in 1 second intervals up to 59 seconds, 15 second
intervals from 1 to 5 minutes, and 1 minute intervals from 5 to 360
minutes. During a run, the display indicates time remaining in minutes.
Below 10 minutes, the time is displayed in minutes and seconds. The run
timing accuracy is better than 10 milliseconds.
Two timing modes are available: Acc, for countdown to start at the
beginning of acceleration; and SPd, for countdown to start when the rotor
has reached 95% of set speed. Press the time down arrow key to scroll
below zero. Acc or SPd will appear, indicating the current timing mode.
Press and release the time down arrow key to toggle between Acc or
SPd. With the correct timing mode in the time display, press the time up
arrow key to select the run time. If the time up key is not pressed at this
time, the originally programmed time will be retained. Acc and SPd only
toggle when the down key is pressed. If a program has been recalled
from memory, altered, and not saved, the manual timing mode is the
same as the original program.
The rotor/radius display indicates either the selected rotor number or the
rotor radius in centimeters. This display illuminates when the rotor is
selected. The applicable IEC rotor numbers are supplied in the memory,
along with their maximum or most common radius in centimeters. The
key under rotor/radius toggles between the two. To select a rotor
number, toggle to ROTOR and press an arrow key under the rotor
display. To change the radius, toggle to RADIUS, and press an arrow
key under the rotor display. Note that the radius cannot be changed to a
radius larger than the maximum radius for that rotor. The display
changes back to rotor number after three seconds.
RPM
RCF
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Centra-GP8/GP8R Operation Manual 9
are lit, to the slowest acceleration, when only one indicator is lit.
The tortoise and hare beside this symbol control rotor braking. Six brake
profiles are available. Press the tortoise to decrease braking, the hare to
increase. As each level is selected another indicator lights up until all
indicators are lit. The sixth profile, coast from set speed, is indicated
when all lights are out.
The profiles for acceleration and deceleration are shown in the graphs
below. Press the tortoise and hare buttons to select the rates which are
most appropriate for your application. For example, for delicate
separations, use Accel 1 (slow) and a coast mode.
Gentleaccelerationandbraking can beselectedwhen centrifuging
delicate samples. The gentle settings prevent the mixing of density
gradients and the breakup of pellets. The yellow acceleration and
decelerationmodeLED’sindicatewhich rates have been chosen(one
LED equals setting of 1-slow; five LEDs equals setting of 5-fast).
The tortoise and hare beside this symbol control rotor acceleration to 300
RPM. After 300 RPM, acceleration is always at maximum rate. Press
the tortoise to decrease acceleration, the hare to increase. Five accel.
profiles are available, ranging from fast acceleration, when all indicators
ACCELERATION AND DECELERATION RATES
0 10 20 30 40
0
100
200
300
400
Accel. 5
Accel. 4
Accel. 3
Accel. 2
Accel. 1
RPM
Time (sec.)
GP8/R Acceleration Rates
0 10 20 30
0
200
400
600
800
1000
1200 5
4
3
2
1
Time
RPM
Full Brake
GP8/R Deceleration Rates
Full Brake; Half Brake From 250 rpm
Full Brake; Coast From 250 rpm
Full Brake; Half Brake From 500 rpm
Full Brake; Coast From 500 rpm
5 4 3
21
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Centra-GP8/GP8R Operation Manual
10
This button saves the currently displayed setting as stored program 1
through 35. (see section 3.5)
This button starts a run, using the desired settings shown on the display
panel. The associated green light blinks while the rotor approaches the
set speed. Then the light stays on until the end of the run.
This button stops the run. (A run will also stop when the set time has
elapsed.) The associated red light blinks as the rotor decelerates. (It also
blinks if an error occurs; see section 3.7) The light stays on when the
rotor stops. Three beeps signal that the rotor has stopped.
This button unlocks the cover. The button is inoperative during a run, and
the cover will not unlock until the rotor speed is below 90 rpm. The cover
must be closed to start a run. The associated yellow light is on whenever
the cover is open.
3.2 Rotor and Accessories
Balance A balanced load is essential for the safe operation of all centrifuges.
An unbalanced load produces vibration and can damage the unit. A 2
gram load imbalance, at a speed of 4600 rpm, imparts a force equivalent
to 9.1 kg at rest (20 pounds). Therefore, always make sure that the rotor
is loaded symmetrically with a full complement of accessories, and with a
full (or paired) set of tubes. Tube adapters should also be installed
symmetrically.
IEC rotors are dynamically balanced at the factory. IEC matches
removable parts (trunnion rings, shields, buckets and carriers) to within
1.0 gram, and stamps the weight on each piece. Check these markings
whenever you interchange parts to ensure that opposite parts are
matched in weight. The total weight of samples and removable parts
loaded in opposing positions must be equal in weight to within 1.0 gram.
The position numbers, present on many rotors and adapters, identify
opposingtubepositions.
To obtain good dynamic balance, the opposite loads must not only be
equal in mass, but must also have the same center of gravity. Opposing
containers must be alike in shape, thickness and distribution of glass or
plastic. This is especially important for large containers. Tubes loaded
into swinging bucket rotors must likewise be symmetrical around the axis
of rotation. Verify this by rotating the entire rotor 180° by hand: the loads
should be in the same apparent positions (not in the mirror image). In
addition, the loads within each bucket must also be symmetrical around
the bucket’s pivot axis. Verify this by ensuring that each bucket is loaded
and doesn’t tilt vertically when the rotor is at rest. Maintaining balance
within each bucket ensures that the bucket and the tubes swing out
horizontallywhenthe rotorreachesoperating speed,applyingcentrifugal
force to the bottom of the tubes. Failure to achieve full swing-out causes
vibration,prematurewearand may resuspendsamples.
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Centra-GP8/GP8R Operation Manual 11
For example, load tubes in the
followingmanner:
1. Load four tubes.
Positions 3, 6, 10, 13
or 2,5,9,12
or 1,4,8,11
2. Load six tubes.
Positions6,7,3,13,14,10
or5,7,2,12,14,9
or1,7,4,8,14,11
3. Load an odd number of tubes.
Not recommended (unless a
dummy tube is used for balance.)
Samples of different specific gravities may be processed in the same run,
provided that the samples of a given type are balanced around the rotor
as though they were the only ones in the rotor.
Vibration All centrifuges have critical speeds at which vibration occurs.
As the speed increases beyond the critical speed vibration will cease.
This inherent condition also occurs during deceleration. An imbalanced
load intensifies these critical vibrations. Do not operate this centrifuge
continuously at observed critical speeds.
Rotor Installation The rotors specified for this centrifuge are secured to the drive motor
shaft by a hex nut which is tightened or loosened with the IEC Part No.
1787 wrench, which is supplied with the machine. Before placing the
rotor onto the shaft, remove the hex nut, then be sure that the tapered
hole in the rotor and the taper of the shaft are clean. Taking care not to
damage the threads, carefully lower the rotor onto the shaft, then tighten
the hex nut to secure the rotor to the motor shaft. NOTE: Do not use
any other tool to tighten the hex nut as overtightening could occur
and the threads could be damaged.
Rotor Removal Use the IEC Part No. 1787 wrench to loosen the hex nut which holds the
rotor to the shaft. Remove the nut by turning it counterclockwise. Use
both hands to keep the rotor horizontal and pull the rotor up and off the
shaft. Periodic lubrication of the taper with BLC (see Section 5.1) will
assureeasyrotorremoval.
Adding Rotors As new rotors are released by IEC, the rotor number and radius can be
added to the rotor menu. To do so, press the hidden key located behind
the “C” in IEC. Prog x.x will appear. Press RPM arrows until "rotor Add"
appears. Press the file key and "Rotor" will appear next to the Rotor/
Radius display. Press an arrow key under the rotor display to select the
new rotor number. Press the file key. "Radius" appears. Use arrow keys
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Centra-GP8/GP8R Operation Manual
12
to select maximum radius. Press file key again. "RPM" will be
illuminated. Use the RPM arrow key to select the maximum RPM for the
rotor. Push file again. Unit beeps three times to acknowledge addition of
rotor. Press "C" in IEC to return to normal operation. Note: Up to five
rotors may be added to memory. When the rotor memory is full, the unit
displays "FUL" under the rotor symbol.
Deleting Rotors Push "C" in IEC. Use RPM arrows to scroll to "rotor Add." Use the
time arrows to select "dEL" instead of "Add." Press file key. "Rotor"
appears. Use rotor arrows to select rotor to be deleted. Press file key to
delete. Unit beeps three times to acknowledge deletion of rotor.
3.3 Starting and Stopping a Run
Install as described in Section 2 of this manual. Plug in the power cord.
For models with a circuit breaker, ess the green button (see Section 2.3).
Read section 3.1 for a general description of the front panel. The desired
settings (press arrow key to display momentarily) shown on the front
panel always govern the operation of the unit. The number or symbol
displayed above the PROGRAM keys describes the operating mode of
the unit. It’s important that the unit be in the correct mode for the desired
operation(seeSection3.4).
Starting A Run Press the On/Off switch in the lower right corner of the front panel.
The Front Panel displays the set parameters for Speed, Time,
Temperature(GP8R),ProgramNumber, and Accelerationand
Deceleration(brake)settingsandrotor number current when themachine
was turned off. The stop indicator is illuminated showing that the machine
is not running. After 3 seconds the display changes to the actual
parameters.
Press the cover button to open the Centra-GP8, and install a rotor per
rotor installation instructions. To close the cover, lower the centrifuge
cover to approximately 6 inches open. With a slight flick of the wrist,
firmly push down the cover so that the resulting momentum engages the
latches. Since the GP8 series has a two-point interlock system, both
mechanisms must be fully engaged for operation to proceed. If the run
button is pressed when only one interlock is engaged, a "Lid" message
will appear in the speed display. Should this occur, close the cover
again. The yellow lid light turns off when the cover is fully latched.
Press the arrow keys twice to start changing each parameter. The key
may be pressed repeatedly or held to increment the parameter. Press
the accel or decel key to select the appropriate acceleration and braking
rates. Press START. A three beep signal sounds, the start indicator
blinks and the display changes to the set parameters. After 1.5 seconds
the run starts and the display changes back to the actual run parameters.
To view the set parameters for three seconds, press and release any
arrow key at any time during a run.
Stopping A Run A run will end when the set time expires, or press the stop button
to end a run and begin deceleration as selected.
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Centra-GP8/GP8R Operation Manual 13
3.4 Operating Modes
The PROGRAM symbol can be one of the following operating modes:
blank: The unit is set to manual operation.
1-35: The unit is under control of the stored program with the number
shown. (see Section 3.5)
C: The unit is set to Rapid Condition.
H: The unit is set to hold mode, in which it runs until you stop it.
These digits and symbols appear above the PROGRAM arrows when the
arrowsare pressed.
Whenever you change the unit’s mode of operation, the rest of the front
panel assumes the state it was in the last time that mode was selected.
Parameters may be changed during a run in manual mode or HOLD
mode.Temperature andacceleration/decelerationsettingscanbe
changed during Rapid Condition. The centrifuge will adjust to the new run
parameters when the display changes back to run mode 3 seconds after
the last key is released.
Manual Operation Press the PROGRAM keys until the PROGRAM display is blank. Next,
select a desired temperature, rpm (or RCF, if rotor number is selected),
run time, acceleration, rotor/radius, timing and deceleration modes. Then
press START. The spin stops automatically at the end of the desired
interval. To manually stop the spin, press the STOP button.
During manual operation, the MINUTES display counts down the
remaining time in the current spin.
When the timing mode is ACC, the run time that you specify includes
acceleration time and begins when you press the START button. When
the timing mode is SPd, count down starts when 95% of set speed is
reached. Deceleration begins when the specified time elapses.
You can change the settings during a manual run to affect the run in
progress. If you change the time settings, the unit adjusts the display
countdown accordingly. If the revised run time is less than the current
time remaining, reducing the time setting may end the run. You cannot
change the unit's program, rotor/radius, or timing modes during a spin.
Rapid Condition When the chamber temperature is above the set temperature, RAPID
(GP8R Only) CONDITION will run a rotor at 500 rpm to increase air circulation in the
chamber to quickly cool the chamber to the set point. When the chamber
temperature is below the set temperature, RAPID CONDITION will run
the rotor at 3400 rpm to warm the chamber to the set temperature. When
the temperature has been reached a three beep signal will sound and the
rotor will brake to rest. (Some smaller rotors may not be able to warm the
chamber to the higher temperature settings.)
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Centra-GP8/GP8R Operation Manual
14
Hold Mode In HOLD mode the centrifuge will run at the current settings for speed
and temperature until the STOP button is pressed. The time display will
count up until STOP is selected. The run time display is retained until the
lid is opened and closed again. HOLD uses the current settings for
acceleration and braking. Set parameters can be changed during a run in
the HOLD mode.
3.5 Stored Programs
The Centra-GP8 series has an internal memory capable of holding 35
sets of run parameters. Each set, or program, is stored and can be
recalled by selecting a program number (1-35). Programs are retained in
memory even if the power is turned off. When necessary, a program can
be modified for a particular run or changed permanently. You cannot
change the unit's program, rotor/radius, or timing modes during a spin.
Locking Programs Programs can be locked from the program lock in the special function
menu. Press the "C" in IEC. Use RPM arrows to scroll until "Loc P" is
displayed. Use the program arrow keys to select the program to be
locked. Pressing the following keys in the following order will lock or
unlock a program: Start, Stop, Cover Open, Stop, Start and File (Save).
The display will alternate between the program number and an "L"
indicating that the values of the program are locked and cannot be
changed.
Recall Program Press a program arrow key to select the appropriate program number.
The programmed run parameters will be displayed and will become the
set parameters. To begin this run, simply press START.
Add/Change Program Select a program number with the program arrow keys. The current
program parameters will appear on the display. Modify the desired
parameters using the parameter arrow keys, or the ACCEL or BRAKE
switches. To make the changes permanent, press the PROGRAM SAVE
(file folder) key. The program number will stop flashing, and the new
program will be displayed and will remain in memory until further changes
are made. To make changes temporary, press START without pressing
the PROGRAM SAVE (file folder) key. The program display goes blank
to indicate that the values are now stored in the manual program and the
instrument is not operating from the program mode at this time. As long
as the PROGRAM SAVE (file folder) key is not pressed, the original
programremainsunchanged.
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Centra-GP8/GP8R Operation Manual 15
3.6 Refrigeration (GP8R Only)
Whenever the cover is closed and the unit is switched ON, the rotor
chamber is cooled as necessary to maintain the desired temperature
setting. However, using the keyboard so that a cold temperature is
momentarilydisplayed(forexample, stepping through thestored
programs)doesnot activaterefrigeration.
If a temperature higher than ambient is specified, the unit does not heat
the rotor chamber except through the normal heating effect of the
equipment (i.e. rotor air friction).
If the rotor chamber is not at the temperature specified, it does not abort
the spin. However, if the rotor chamber differs by 5°C or more from the
specified temperature at the start of a run, the unit sounds an audible
alarm. The °C display switches between the actual and programmed
temperature until the two temperatures come within 5°C. This shows the
reason for the alarm. Press the STOP button if the run should not
continue at the actual temperature.
The unit is not designed for use as a refrigerator. The natural fanning
action of the rotating horizontal and fixed angle rotors serves to maintain
a uniform temperature distribution inside the chamber. Therefore, at zero
RPM, set and actual chamber temperatures may be different.
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Centra-GP8/GP8R Operation Manual
16
3.7 Fail-Safe Systems
Interlock: Lid cannot be opened when motor is energized or when rotor
speed exceeds 90 rpm. The lid latch is a mechanical latch opened by
momentary engagement of a solenoid. In the event of a power failure, the
door latch can be actuated with a tool for sample recovery.
User Diagnostics: Warning messages will appear in the display and
seven beeps will sound to alert the user of conditions that require
attention:
bAL This message appears in the time display when an imbalanced rotor
is run. The rotor decelerates with full brake to rest. The cover must be
opened to reset this warning.
LId This message appears in the time display If the lid is not fully closed
when START is pressed.
HEAd This message warns that a run has been started with no rotor in
the chamber. The cover must be opened to reset this warning.
PFAIL A power failure was detected during a run. Rotor will be stopped at
programmeddecelerationwhenpoweris restored. This warningis
cleared by opening the cover.
bruSh This message appears (every 800 hours of use) at the end of
every run if it is time to check or change the brushes.
Error Codes: Software will monitor and provide appropriate control,
including front panel error messages in the time display, when any of the
followingconditionsoccur:
Error 001: No tachometer
Error002: Overspeed
Error003: Runaway
Error 004: Chamber temperature in excess of 45°C.
Error 005: Fail-safe time-out
Error006:COPWatchdog/Op-Code Trap error
Error 007: Stack error
Error 008: No COP - COP watchdog system not active
Error009:Undefined interrupt
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Centra-GP8/GP8R Operation Manual 17
ROTOR 216 4-place Swinging Bucket Rotor
(includes four Cat. No. 316S buckets)
No. of
Places
Tube
Volume
(ml)
Adapter
Number Maximum
Speed (rpm)
Max
RCF
(xg)
Max
Radius
(cm) Max Tube Size
O.D. x Length (mm)
148 0.25/0.4 5737
148 0.5/0.7 5737
148 5 5737
108 1.5 5827
108
76
7-10 5827
10-20 5719
3400
3400
3400
3400
3400
3400
3400
36 15 5719 3400
2400
1890
2440
1940
2440
2440
2440
28 40-50 5707 2440
12
12
20
28 50
50
15
50 5703w/323
5703w/7323
5805
5807 3400
3400
3400
3400 2460
2070-2460
2390
2430
19.0
18.8
18.9
18.9
16-19
18.5
18.9
14.6
18.9
15.0
18.9
18.6 0.25/0.4 microtubes
0.5 microtubes/B-D Microtainers
12.4x91
1.5 microtubes
18.0x130
14.5x130
Falcon/Corning plastic conical
29.6x130
29.5x115
29.6x126
17.4x126
29.4x118
850 5704
8 100 5704
4140 5780
4 250 5780
4
4
500 5781
750 -
3400
3400
3400
3400
3400
3400
3400
4Blood
Bags 2039
3400
2380
2530
2460
2550
2530
2550
2520
4Micro-
plates 5782 2210
4Cyto-
slides
5799w/1024 1500
19.5
17.1
19.0
19.7
19.6
19.0
19.7
19.6
18.4 29.5x125
44.8x136
63.4x143
63.4x138
98.3x138
77.1x145
Single, double pack bags
86Wx128L
25Wx75L
480
4 ACCESSORIES
4.1 Speed And ForceTables
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Centra-GP8/GP8R Operation Manual
18
ROTOR 218 4-place Swinging Bucket Rotor
(includes windshield cover and four Cat. No. 3218 sealed b
No. of
Places
Tube
Volume
(ml)
Adapter
Number Maximum Speed (rpm)
Max
RCF
(xg)
Max
Radius
(cm) Max Tube Size
O.D. x Length (mm)
148 0.25 5737
148 0.4 5737
148 0.5 5737
108 1.5 5827
76
148
45719
3-5 5737
4600
4600
4600
4600
4600
4600
4600
108 7-10 5827
4600
3850
4300
4260
4140
4330
4330
4330
76 10-15 5719 4330
12
24
48
28 15
15
15
12 5703
5712
5712
5719 4600
4600
4600
4600 4330
4330
4330
4330
18.3
18.3
18.3
18.3
18.3
18.3
18.3
18.2
18.0
17.5
18.3
16.3 0.25 microtube
0.4 microtube
B-D Microtainer
1.5/2.0 microtube
12.4x118
18.0x65
14.5x118
18.0x118
18.0x127
Falcon/Corning Conical
Falcon/Corning Conical
Falcon/Corning Conical
12 15 5703
28 40-50 5707
20 50 5805
12 50 5703
12
4
40-50 5703
150 5780
4600
4600
4600
4600
4600
4600
4600
4 200 5780
4600
4400
4330
4470
4400
4400
4450
4470
4225 5780 4470
4 250 5780 4600
18.9
18.9
19.2
18.8
18.3
18.9
18.6
18.6
18.6 17.5x126
29.6x118
Falcon/Corning Conical
Falcon/Corning Conical
63.4x139
29.4x118
63.4x137
63.4x139
63.4x139
4550
4 500 5781 4000
1500
4Blood
Bags 2077
4600
4550
4470
4750 -4550
4Plates 5782 4000
18.9
19.2
14.8
19.2 77.3x145
Single, double pack
98.4x140
86x128
2650
4Slides 5799 1500
18.4 Microscope slides
460
Rotor 218A 4-place Swinging Bucket Rotor
(includes windshield, cover and four cat. no. 3218 buckets)
24
140
175/225
5784
4600
4600
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