Thermo Scientific EVOS M5000 User manual

EVOS™M5000 Imaging System
Catalog Number AMF5000SV
Pub.No. MAN0017783 Rev. C.0
Note: For safety and biohazard guidelines, see the “Safety” appendix in the following product documentation: EVOS™M5000 Imaging
System User Guide (Pub.no.MAN0017563). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate
protective eyewear, clothing, and gloves.
Standard items included
EVOS™M5000 Imaging System
EVOS™M5000 Imaging System, includes the following components and pre-installed accessories:
• 18.5 in articulated LCD monitor (1920 ×1080 pixel resolution)
• Embedded PC (4GB RAM)
• Manual X-Y stage with position-sensing technology
• 5‑position objective turret
• Condenser with 4‑position turret
• Light cube shipping restraint (remove before use)
• Stage lock pin (remove before use)
• EVOS™Condenser Light Shield
• Light cube tool (remove before use)
• Blank light cube (remove before use)
• LED light cubes, as ordered
• Objectives, as ordered
EVOS™M5000 Accessories Kit
EVOS™M5000 Accessories Kit (located in the instrument box), contains:
• Wireless mouse and keyboard
• USB receiver (for wireless mouse and keyboard connection)
• USB Wi-Fi adaptor (for wireless network connection to Connect applications and mapped network drives)
• USB 3.0 flash drive, 16 GB (for image storage, preloaded with user documentation)
• EVOS™Vessel Holder, Multiwell Plate (Cat. No. AMEPHV022)
• EVOS™Vessel Holder, Two 25 × 75 mm slides (Cat. No. AMEPVH001)
• EVOS™Vessel Holder, Universal (Cat. No. AMEPVH009)
• EVOS™Calibration Slide (Cat. No. AMEP4720)
• EVOS™Light Shield
• UV shield assembly
• Condenser Slider, Block (Cat. No. AMEP4688)
• Condenser Slider, 4X Pupil (Cat. No. AMEP4738)
• Condenser Slider, Diusion for Brightfield Applications (Cat. No. AMEPDFS1)
• Universal power supply (12 V, 5 A) and power cord (type B, North America)
• EVOS™Dust Cover
• Accessories box with adjustable compartments
INSTALLATION GUIDE
For Research Use Only. Not for use in diagnostic procedures.

• Hex key, 2mm
• Mouse pad
EVOS™M5000 Imaging System user documentation
• EVOS™M5000 Imaging System Quick Start Guide, printed (Pub. No. MAN0017765)
• EVOS™M5000 Imaging System Installation Guide, printed (Pub. No. MAN0017783)
• USB 3.0 flash drive (located in the accessories box) contains:
– EVOS™M5000 Imaging System User Guide (Pub. No. MAN0017763)
– EVOS™M5000 Imaging System Quick Start Guide (Pub. No. MAN0017765)
– EVOS™M5000 Imaging System Installation Guide (Pub. No. MAN0017783)
Note: Contact your distributor if anything is missing. If you do not have your distributor information, contact Technical Support. Damage
claims must be filed with the carrier; the warranty does not cover in-transit damage.
Operating environment and site requirements
• The dimensions of the EVOS™M5000 Imaging System are 18 × 23 × 18 in (W × H × D) (46 × 59 × 46 cm). The system requires a
benchtop of approximately 36×36in (92×92cm).
• If the system includes the optional EVOS™Onstage Incubator (Cat. No. AMC2000), then add 16 in (40 cm) to the width of the bench.
• Allow at least 5 cm (2 in) free space at the back of the instrument to allow for proper ventilation and prevent overheating of electronic
components.
• Place the EVOS™M5000 Imaging System on a level surface away from vibrations from other pieces of equipment. Tabletop
centrifuges, vortex mixers, and other laboratory equipment can vibrate the instrument during a run and cause a decrease in
instrument performance.
• The EVOS™M5000 Imaging System should be installed away from direct light sources such as windows. Ambient light can enter the
imaging path and aect the image quality.
• Operating temperature range: 4°–32°C (40°–90°F).
• Relative humidity range: 30–90%.
• Operating power: 100–240 VAC, 1.8A
• Frequency: 50–60Hz
• Electrical input: 12VDC, 5A
IMPORTANT! Do not position the instrument so that it is dicult to turn o the main power switch located on the back of the instrument
base. In case of an instrument malfunction, turn the main power switch to the OFF position and disconnect the instrument from the wall
outlet.
Cell culture hood set up
The EVOS™M5000 Imaging System fits in cell culture hoods that are at least 24in (61cm) deep and 36in (92cm) high with a 30in
(76 cm) opening. If your cell culture hood is smaller, it may be possible to fit the instrument by turning it at a slight angle.
2EVOS™M5000 Imaging System Installation Guide

Instrument exterior components and mechanical controls
Front view
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2
3
54
6
11
8
8
7
10
9
1LCD monitor
2Condenser
3Condenser light shield
4Vessel holder and thumb screws
5Mechanical X-Y stage
6Stage X- and Y-axis positioning knobs
7Objective selection wheel
8Focusing knobs
9USB-A 3.0 port
10 Objective
11 Phase ring selector
EVOS™M5000 Imaging System Installation Guide 3

Rear view
1LCD monitor
2Mechanical X-Y stage
3Focusing knobs
4USB-A 3.0 port (2×)
5Power switch
6Single-pin power input port (12VDC, 5A)
7DisplayPort (video output)
8Network port
9USB-A 2.0 ports (2×)
10 Stage X- and Y-axis positioning knobs
11 Instrument passwords sticker
Prepare for installation
Receive and inspect the shipment
1. Verify that the items shown on the shipping list are the same items that you ordered at the time of purchase.
2. Carefully inspect the shipping containers and report any damage to the Thermo Fisher Scientific service representative. Record any
damage or mishandling on the shipping documents.
Move the instrument to the installation site
1. Clear the installation site of all unnecessary materials.
2. If possible, move the crated instrument and other shipping containers to the installation site.
CAUTION! PHYSICAL INJURY HAZARD. Lift or move the instrument using proper lifting techniques. We recommend that
you lift or move the crated instrument with the assistance of others and the use of appropriate moving equipment. Improper
lifting can cause painful and permanent back injury. Depending on the weight, moving or lifting an instrument may require two or
more persons.
4EVOS™M5000 Imaging System Installation Guide

Install the instrument
Unpack the instrument
1. Open the shipping box and remove the accessory box.
2. Carefully lift the instrument out of the box by grasping it firmly with both hands under the support arm.
IMPORTANT! Do not subject the EVOS™M5000 Imaging System to sudden impact or excessive vibration. Handle the instrument
with care to prevent damage.
3. Place the instrument on a flat, level surface that will be free from vibration and leave enough room around it for the stage to move
freely.
4. Tilt the LCD monitor upright.
5. Examine the instrument carefully for damage incurred during transit.
6. Unpack the accessories box and verify all parts are present. See “Standard items included” on page1 for the list of standard items
included in the shipment.
Note: Contact your distributor if anything is missing. If you do not have your distributor information, contact Technical Support. Damage
claims must be filed with the carrier; the warranty does not cover in-transit damage.
Note: Make sure to set aside packaging and foam for future transport and storage. Re-install the stage lock pin and the light cube
shipping restraint before moving or transporting the instrument. Always ensure that the instrument is properly cushioned and braced to
prevent damage.
Remove shipping restraints
The EVOS™M5000 Imaging System is equipped with two shipping restraints (stage lock pin and light cube shipping restraint) to protect
the instrument from shock and vibration during transport. You must remove the shipping restraints before you power on the system.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Library.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1
1
1
3
2
1Stage lock pin
2Light cube shipping restraint
3Light cube tool
1. Pull firmly to remove the stage lock pin and release the X- and Y-axis brakes.
2. Using the X-axis and Y-axis stage positioning knobs, move the stage back to obtain access to the light cube shipping restraint,
which is centered under the back of the stage.
Note: The light cube shipping restraint is secured with the light cube tool to the blank light cube installed in the light cube turret.
Used together, they immobilize the light cube turret to protect it during transport.
EVOS™M5000 Imaging System Installation Guide 5

Figure1Before stage reposition
Figure2After stage reposition
3. Unscrew and remove the light cube tool, which secures the shipping restraint block to the blank light cube.
4. Remove the shipping restraint block and store it in the accessories box. Removal of the restraint block provides access to the blank
light cube in the light cube turret.
Note: The blank light cube is red and does not have the grooved copper top of the LED light cubes.
1Blank light cube
2Thread hold
3Screws
5. Using the light cube tool, loosen the two screws that secure the blank light cube to the instrument. You do not need to remove the
screws.
6. Screw the light cube tool to the thread hold on the light cube, then lift the blank light cube up and out of the light cube turret. Store
the blank light cube and the light cube tool in the accessories box.
7. Place the desired EVOS™Vessel Holder on the stage.
Note: Store the shipping restraints and the light cube tool for future use in the accessories box provided with your system. Always
re-secure the X-Y stage with the stage lock pin and re-install the light cube restraint before moving the instrument.
IMPORTANT! Before changing light channels, ALWAYS verify the light cube restraint has been removed. Attempting to change
the light channels while the restraint is in place can seriously damage the mechanism. This type of damage is not covered by the
manufacturer’s warranty.
6EVOS™M5000 Imaging System Installation Guide

Install the UV light shield
1. Verify that the EVOS™Condenser Light Shield is installed on the condenser assembly. The condenser shield is pre-installed and
helps reduce the potential eects of overhead lighting on your image.
2. Pull the condenser light shield out from the condenser head.
3. Secure the UV light shield mount to the top of the condenser light shield using the two screws supplied with the UV shield assembly
(not the protruding screws on the mount).
4. Clip the condenser light shield with the attached UV light shield mount back onto the condenser head.
EVOS™M5000 Imaging System Installation Guide 7

5. Peel the protective paper from the UV light shield, then slide the orange UV light shield over the two protruding screws on light shield
mount to attach it to the instrument.
Note: The UV light shield is provided as a safety feature and should be installed whenever the unit is in operation. The UV light shield
is removable for access to the condenser sliders used in transmitted light mode. Simply unhook it from the screws on the UV light
shield mount.
Connect the instrument
1. Confirm that the power switch is OFF (located on the back; see “Rear view” on page4).
2. Connect the USB receiver for the wireless mouse and keyboard to an available USB-A 2.0 port (located on the back; see “Rear
view” on page4).
3. Connect the power adapter and power cord. Ensure a tight connection.
4. Plug the power adapter into the power input port on the instrument (see “Rear view” on page4).
5. Plug the power cord into a power outlet and check for the light on the power supply.
8EVOS™M5000 Imaging System Installation Guide

Power on the EVOS™M5000 Imaging System
1. Turn the instrument power switch (located on the back; see “Rear view” on page4) to the ON position.
2. When the Capture tab is displayed, the EVOS™M5000 Imaging System is ready to use.
IMPORTANT! You must remove all shipping restraints before you power on the EVOS™M5000 Imaging System to prevent damage
(“Remove shipping restraints” on page5).
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Revision history:Pub.No.MAN0017783 C.0
Revision Date Description
C.0 14 November 2023 Add Stage View tool and update screens and content.
B.0 25 January 2019 Update image of capture screen.
A.0 27 August 2018 New installation guide for EVOS™M5000 Imaging System.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all
applicable Limited Use Label Licenses.
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14 November 2023