Thermo Scientific iBright CL750 User manual

For Research Use Only. Not for use in diagnostic procedures.
iBright™CL750 Imaging System
USER GUIDE
For use with the iBright™CL750 Imaging System
Catalog Numbers A44116
Publication Number MAN0018652
Revision C.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018652
Revision Date Description
C.0 24 August 2020 Adding SAE chapter due to addition of SAE instrument functionality.
B.0 23 September 2019
•Updated the components list (see Table 1 on page 6).
•Added web link to interactive training demonstrations (see “Product description” on page 6).
•Updated the emission filters for imaging in Universal mode (see “Universal Mode” on page 16).
•Added additional outputs for TPN-normalized data.
•Added additional information for resetting factory defaults, backing up, and restoring settings (see
“Settings” on page 27).
A.0 June 7 2019 New document. Describes installation, operation, and maintenance of the iBright™Imaging Systems.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2020 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■Product information ................................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 6
■Start, sign on, and configure the instrument ....................................... 8
Create an instrument profile ...................................................... 8
Create an instrument account and link to Connect using a cloud account .............. 8
Mobile device .............................................................. 9
PC using Connect account ................................................... 9
PC using instrument Connect app ............................................ 9
Use instrument to link to Connect account .................................... 10
Link to Connect using an existing instrument account .............................. 10
If the unit is o ................................................................. 10
If the unit is on and in sleep mode ................................................ 11
If the unit is on and in active mode ............................................... 11
■Operate the iBright™imager ........................................................ 12
Choose imaging mode and start image capture .................................... 12
Chemi Blots ................................................................... 13
Image using Chemi Blots ................................................... 13
Nucleic Acid Gels .............................................................. 14
Image using Nucleic Acid Gel ............................................... 14
Protein Gels ................................................................... 15
Image using Protein Gels ................................................... 15
Universal Mode ................................................................ 16
Options available in modes ...................................................... 17
Image Gallery .................................................................. 20
View images .............................................................. 20
iBright™CL750 Imaging System User Guide 3

■Export and analyze images ......................................................... 21
Export images ................................................................. 21
Export new images ........................................................ 21
Export gallery images ...................................................... 21
Select an Export file type ................................................... 22
Archive/Backup g2i image files .............................................. 23
Analyze images ................................................................ 24
Analyze new images ....................................................... 24
Analyze gallery images ..................................................... 26
■Maintain the instrument ............................................................. 27
Settings ....................................................................... 27
Manage accounts .......................................................... 29
Run instrument diagnostics ..................................................... 30
Clean the instrument ........................................................... 30
Replace the instrument fuses .................................................... 31
Materials required .......................................................... 31
Replace the fuses .......................................................... 31
Move the instrument ........................................................... 32
Prepare for shipping ....................................................... 33
■Invitrogen™iBright™SAE Software Solution for 21 CFR Part 11 Support ..... 34
Security Audit e-Signature (SAE) for the iBright™Imager ............................ 34
Activate your license key ........................................................ 35
Enable SAE mode .............................................................. 35
Disable SAE mode ............................................................. 36
Sign in to the instrument in SAE mode ............................................ 36
Change your password in SAE mode ............................................. 37
SAE user roles ................................................................. 37
SAE privileges for default user roles .............................................. 38
Non-SAE user roles ............................................................ 38
Acquire images in SAE mode .................................................... 39
Use the Gallery in SAE mode .................................................... 39
File status ................................................................ 39
Filter Gallery by file status .................................................. 39
Archive/Backup g2i image files in SAE mode ................................. 39
Commit changes ............................................................... 40
Sign data ..................................................................... 41
Unlock files ................................................................... 42
Contents
4iBright™CL750 Imaging System User Guide

Generate an audit history ....................................................... 42
View the e-Signature history ..................................................... 43
Export image files .............................................................. 43
Generate and export Analysis Report ............................................. 43
■Instrument specifications ........................................................... 44
Instrument dimensions and specifications ......................................... 44
Electrical requirements ......................................................... 44
Environmental requirements ..................................................... 45
Network requirements .......................................................... 46
Instrument and computer connections ............................................ 47
■Safety ................................................................................. 49
Symbols on this instrument ..................................................... 49
Safety alerts on this instrument .............................................. 50
Location of safety labels on the instrument .................................... 51
Safety information for instruments not manufactured by Thermo Fisher Scientific ...... 51
Instrument safety .............................................................. 51
General ................................................................... 51
Physical injury ............................................................. 52
LED (Light-Emitting Diode) .................................................. 52
Electrical safety ........................................................... 53
Cleaning and decontamination .............................................. 54
Instrument component and accessory disposal ................................ 54
Safety and electromagnetic compatibility (EMC) standards .......................... 55
Safety compliance ......................................................... 55
EMC ..................................................................... 55
Environmental design ...................................................... 56
Chemical safety ................................................................ 58
Biological hazard safety ......................................................... 59
■Documentation and support ....................................................... 60
Related documentation and references ........................................... 60
Customer and technical support ................................................. 61
Limited product warranty ........................................................ 61
Contents
iBright™CL750 Imaging System User Guide 5

Product information
IMPORTANT! Before using this product, read and understand the information in
the “Safety” appendix in this document.
Product description
The iBright™CL750 Imaging System allows users to image chemiluminescent
Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and
visible protein gels.
The iBright™imager uses a simple design and intuitive workflows to deliver high-
resolution images. It can be run directly from the touchscreen to start and create
images, while also providing on-board software for image analysis. To further analyze
data, the instrument can be integrated with the iBright™Image Analysis Software
available for desktop and Connect.
Go to thermofisher.com/ibrightcl750training for interactive visual demonstrations
of how to install, operate, and maintain your instrument.
Contents
Table 1 Included imager system parts
Component Quantity
iBright™Imaging System 1
Power cord (region specific) 1
Quick Reference Guide 1
White transilluminator screen 1
iBright™Imaging System Sample Blot 1
Sample stage/turntable 1
Product Information Sheet 1
Safe Imager™Viewing Glasses 1
6iBright™CL750 Imaging System User Guide

Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage A33829
High-Power USB Wi-Fi Module A26774
Product information
Contents
iBright™CL750 Imaging System User Guide 7

Start, sign on, and configure the
instrument
Create an instrument profile
1. Touch to sign in to your instrument user account.
2. Touch Get started.
3. Touch Create a Profile.
4. Enter a Screen name.
5. Enter and confirm a four-digit pin.
6. Touch Create profile.
7. (Optional) If you want to link your instrument to your Connect account, see “Use
instrument to link to Connect account” on page 10 or “Link to Connect using
an existing instrument account” on page 10.
Create an instrument account and link to Connect using a
cloud account
1. Touch to sign in to your instrument user account.
2. Touch Get Started.
3. Click on Connect.
4. Link your account using one of these methods:
•Mobile device — Connect using a QR generated by the instrument and
scanned by the instrument Connect mobile app.
•PC — Connect using a link code that is entered into the instrument Connect
app or your online Connect account.
•Instrument — Enter your Connect account information directly on the
instrument.
8iBright™CL750 Imaging System User Guide

Mobile device
1. Touch Mobile Device.
2. Launch instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on QR code.
6. Scan QR code on the instrument.
Your Connect account should now be linked to your instrument account.
PC using Connect account
1. Touch PC.
2. Login to your Connect account online.
3. Click on
.
4. Click on .
5. Select iBright from the drop-down menu.
6. Enter the linking code and click on Send.
Your Connect account should now be linked to your instrument account.
PC using instrument Connect app
1. Touch PC.
2. Launch the instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on Linking code.
6. Enter the linking code and click Send.
Your Connect account should now be linked to your instrument account.
Start, sign on, and configure the instrument
Create an instrument account and link to Connect using a cloud account
iBright™CL750 Imaging System User Guide 9

Use instrument to link to Connect account
1. Click on Instrument.
2. Enter your Connect username and password.
3. Click Link account.
Your Connect account should now be linked to your instrument account.
Link to Connect using an existing instrument account
Linking to Connect after account creation can only be done during the export
process.
1. Click on .
2. Select an image.
3. Click on Actions.
4. Click on Export.
5. Click on Choose destination.
6. Click on Cloud.
7. Click on Sign in.
8. Connect using one of the methods outlined in “Create an instrument account
and link to Connect using a cloud account” on page 8.
If the unit is o
1. Ensure the unit is plugged in and turn on using the switch on the back of
instrument.
The instrument will go directly to the sign-in page.
2. Enter a Screen name.
3. Enter and confirm a 4-digit pin.
Note: For those not logged on as a user (guest), acquired images are saved to
a guest gallery. The guest gallery is not private. Any logged in user can view and
modify images residing in the guest gallery.
4. From the Welcome screen, choose the appropriate Mode from the drop-down
menu.
Start, sign on, and configure the instrument
Link to Connect using an existing instrument account
10 iBright™CL750 Imaging System User Guide