Thermo Scientific iBright CL1500 User manual

For Research Use Only. Not for use in diagnostic procedures.
iBrightImaging Systems
USER GUIDE
For use with the iBrightCL1500 Imaging System and the iBright
FL1500 Imaging System
C
Catalog Numbers
A43678, A44114, A44240 (CL1500) and A43679, A44115, A44241 (FL1500)
Publication Number
MAN0018592
Revision B.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to t
thermofisher.com/symbols-definition
.
The information in this guide is subject to change without notice.
DISCLAIMER
: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,
INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING YOUR USE OF IT.
Revision history:
Pub. No. MAN0018592
Revision Date Description
B.0 23 September 2019
• Updated the components list (see Table 1 on page 6).
• Updated the recommended excitation/emission wavelengths to image fluorescent Western blots
(see “Fluorescent Blots (FL1500 model only)“ on page 14).
• Updated the emission filters for the iBrightFL1500 Instrument (see Table 4 on page 17).
• Added additional outputs for TPN-normalized data.
• Added additional information for resetting factory defaults, backing up, and restoring settings (see
“Settings“ on page 28).
A.0 June 7 2019 New document. Describes installation, operation, and maintenance of the iBrightImaging Systems.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept
the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2019 Thermo Fisher Scientific Inc. All rights reserved.

C
Contents
ŶProduct information ....................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 6
ŶStart, sign on, and configure the instrument ............................ 8
Create an instrument profile ...................................................... 8
Create an instrument account and link to Connect using a cloud account ............... 8
Mobile device ............................................................... 9
PC using Connect account .................................................... 9
PC using instrument Connect app ............................................. 9
Use instrument to link to Connect account ..................................... 9
Link to Connect using an existing instrument account ............................... 10
If the unit is off ................................................................ 10
If the unit is on and in sleep mode ................................................ 10
If the unit is on and in active mode ................................................ 11
ŶOperate the iBrightimager ............................................ 12
Choose imaging mode and start image capture .................................... 12
Chemi Blots ................................................................... 13
Image using Chemi Blots ................................................... 13
Fluorescent Blots (FL1500 model only) ........................................... 14
Image using Fluorescent Blot ............................................... 14
Nucleic Acid Gels .............................................................. 15
Image using Nucleic Acid Gel ................................................ 15
Protein Gels ................................................................... 16
Image using Protein Gels ................................................... 16
Universal Mode ................................................................ 17
Options available in modes ...................................................... 19
Image Gallery ................................................................. 22
View images .............................................................. 22
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Imaging Systems User Guide
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ŶExport and analyze images .............................................. 23
Export images ................................................................. 23
Export new images ........................................................ 23
Export gallery images ...................................................... 23
Select an Export file type ................................................... 24
Analyze images ................................................................ 25
Analyze new images ....................................................... 25
Analyze gallery images ..................................................... 27
ŶMaintain the instrument ................................................. 28
Settings ....................................................................... 28
Manage accounts .......................................................... 30
Run instrument diagnostics ..................................................... 31
Clean the instrument ........................................................... 31
Replace the instrument fuses .................................................... 31
Materials required ......................................................... 31
Replace the fuses .......................................................... 32
Move the instrument ........................................................... 33
Prepare for shipping ....................................................... 33
ŶInstrument specifications ............................................... 34
Instrument dimensions and specifications ......................................... 34
Electrical requirements ......................................................... 34
Environmental requirements .................................................... 34
Network requirements .......................................................... 35
Instrument and computer connections ............................................ 36
ŶSafety ...................................................................... 38
Symbols on this instrument ..................................................... 38
Safety alerts on this instrument ............................................. 39
Location of safety labels on the instrument ................................... 40
Safety information for instruments not manufactured by Thermo Fisher Scientific ..... 40
Instrument safety .............................................................. 40
General .................................................................. 40
Physical injury ............................................................ 40
LED (Light-Emitting Diode) .................................................. 41
Electrical safety ........................................................... 41
Cleaning and decontamination ............................................... 42
Instrument component and accessory disposal ................................ 42
Contents
4
4
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Imaging Systems User Guide

Safety and electromagnetic compatibility (EMC) standards .......................... 43
Safety compliance ......................................................... 43
EMC ...................................................................... 43
Environmental design ...................................................... 44
Chemical safety ................................................................ 45
Biological hazard safety ......................................................... 46
ŶDocumentation and support ............................................. 47
Related documentation ......................................................... 47
Customer and technical support ................................................. 47
Limited product warranty ....................................................... 47
Contents
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Imaging Systems User Guide
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5

P
Product information
IMPORTANT!
ȱȱȱǰȱȱȱȱȱȱȱ
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Product description
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ǰȱȱȱȱȬȱȱȱȱ¢ǯȱȱȱ¢£
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ȱȱȱȱǯ
Contents
Table 1 Included imager system parts
Component Quantity
iBrightImaging System 1
Power cord (region specific) 1
Quick Reference Guide 1
White transilluminator screen 1
iBrightImaging System Sample Blot 1
Sample stage/turntable 1
Product Information Sheet 1
Safe ImagerViewing Glasses 1
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Imaging Systems User Guide

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Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage A33829
High-Power USB Wi-Fi Module A26774
Product information
Contents
iBright
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Imaging Systems User Guide
7

S
Start, sign on, and configure the
instrument
Create an instrument profile
1.
ȱ ȱȱȱȱȱ¢ȱȱȱǯ
2.
ȱȱǯ
3.
ȱȱȱęǯ
4.
ȱȱȱǯ
5.
ȱȱęȱȱȬȱǯ
6.
ȱȱęǯ
7.
ǻǼȱȱ¢ȱȱȱȱ¢ȱȱȱ¢ȱȱǰȱȱȃ
ȱȱȱȱȱȃȱȱȱşȱȱȃȱȱȱȱ
¡ȱȱȃȱȱȱŗŖǯ
Create an instrument account and link to Connect using a cloud
account
1.
ȱ ȱȱȱȱȱ¢ȱȱȱǯ
2.
ȱȱǯ
3.
ȱȱǯ
4.
ȱ¢ȱȱȱȱȱȱDZ
Ȋȱȱȯȱȱȱȱȱȱ¢ȱȱȱ
ȱ¢ȱȱȱȱȱǯ
Ȋȱȯȱȱȱȱȱȱȱȱȱȱȱȱ
ȱȱ¢ȱȱȱǯ
Ȋȱȯȱȱ¢ȱȱȱȱ¢ȱȱ
ǯ
8
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Imaging Systems User Guide

1
1.
ȱȱǯ
2.
ȱȱȱȱǯ
3.
ȱȱ¢ȱȱȱȱȱȱǯ
4.
ȱȱ ǯ
5.
ȱȱȱǯ
6.
ȱȱȱȱȱǯ
ȱȱȱȱȱȱȱȱ¢ȱȱǯ
1.
ȱǯ
2.
ȱȱ¢ȱȱȱǯ
3.
ȱȱ ǯ
4.
ȱȱ ǯ
5.
ȱȱȱȱȬȱǯ
6.
ȱȱȱȱȱȱȱǯ
ȱȱȱȱȱȱȱȱ¢ȱȱǯ
1.
ȱǯ
2.
ȱȱȱȱȱǯ
3.
ȱȱ¢ȱȱȱȱȱȱǯ
4.
ȱȱ ǯ
5.
ȱȱ ȱǯ
6.
ȱȱȱȱȱȱǯ
ȱȱȱȱȱȱȱȱ¢ȱȱǯ
1.
ȱȱǯ
2.
ȱ¢ȱȱȱȱǯ
3.
ȱ ȱǯ
ȱȱȱȱȱȱȱȱ¢ȱȱǯ
Mobile device
PC using Connect
account
PC using
instrument
Connect app
Use instrument to
link to Connect
account
Start, sign on, and configure the instrument
Create an instrument account and link to Connect using a cloud account
iBright

Imaging Systems User Guide
9

Link to Connect using an existing instrument account
ȱȱȱȱȱȱȱ¢ȱȱȱȱȱ¡ȱǯ
1
1.
ȱȱ ǯ
2.
ȱȱǯ
3.
ȱȱǯ
4.
ȱȱ¡ǯ
5.
ȱȱȱǯ
6.
ȱȱǯ
7.
ȱȱȱǯ
8.
ȱȱȱȱȱȱȱȱȃȱȱȱȱ
ȱȱȱȱȱȱȃȱȱȱŞǯ
If the unit is off
1.
ȱȱȱȱȱȱȱȱȱȱȱȱȱȱȱ
ǯ
ȱȱȱȱ¢ȱȱȱȬȱǯ
2.
ȱȱȱǯ
3.
ȱȱęȱȱŚȬȱǯ
Note:
ȱȱȱȱȱȱȱȱǻǼǰȱȱȱȱȱȱ
ȱ¢ǯȱȱȱ¢ȱȱȱǯȱ¢ȱȱȱȱȱȱ
¢ȱȱȱȱȱȱ¢ǯ
4.
ȱȱȱǰȱȱȱȱȱȱȱȬ
ǰȱȱ ȱȱȱȱȱȱȱȱȱȱǯ
5.
ȱ ȱȱȱȱǻȱȱ¢¢ȱȱȱȱǼǯȱ
ȱȱ¢ȱȱȬȱȱȱȱȱǯ
If the unit is on and in sleep mode
1.
ȱȱȱȱǯ
2.
ȱ ȱȱȱȱȱȱȱȱȱȱȱęǯȱȱȃȱȱ
ęȃȱȱȱŞȱȱȱȱȱȱȱȱȱęǯ
Note:
ȱȱȱȱȱȱȱȱǻǼǰȱȱȱȱȱȱ
ȱ¢ǯȱȱȱ¢ȱȱȱǯȱ¢ȱȬȱȱȱȱ
ȱȱȱȱ¢ȱȱȱȱȱȱ¢ǯ
Start, sign on, and configure the instrument
Link to Connect using an existing instrument account
10
iBright

Imaging Systems User Guide

3
3.
ȱȱȱǯ
4.
ȱȱęȱȱŚȬȱǯ
5.
ȱȱȱǰȱȱȱȱȱȱȱȬ
ǰȱȱȱǰȱȱȱȱȱȱȱȱȱ
ǯ
6.
ȱ ȱȱȱȱǻȱȱ¢¢ȱȱȱǼǯȱ
ȱȱ¢ȱȱȬȱȱȱȱȱǯ
If the unit is on and in active mode
1.
ȱȱ¢ȱȱǰȱȱ ȱȱȱȱȱȱȱȱȱȱęǯȱȱ
ȃȱȱȱęȃȱȱȱŞȱȱȱȱȱȱȱȱ
ęǯ
Note:
ȱȱȱȱȱȱȱȱǻǼǰȱȱȱȱȱȱ
ȱ¢ǯȱȱȱ¢ȱȱȱǯȱ¢ȱȬȱȱȱȱ
¢ȱȱȱȱȱȱ¢ǯ
2.
ȱȱȱǰȱȱȱȱȱȱȱȬ
ǰȱȱ ȱȱȱȱȱȱȱȱȱȱǯ
3.
ȱ ȱȱȱȱǻȱȱ¢¢ȱȱȱǼǯȱ
ȱȱ¢ȱȱȬȱȱȱȱȱǯ
Start, sign on, and configure the instrument
If the unit is on and in active mode
iBright

Imaging Systems User Guide
11

O
Operate the iBright

imager
Choose imaging mode and start image capture
ȱȱȱDZ
1.
ȱȱȬȱȱȱȱȱȱǯ
Ȋȱȱȱȱȱǯ
ȊȱȱȱȱĚȱǯ
Ȋȱȱȱȱȱȱȱȱǯ
Ȋȱȱȱȱǰȱȱȱǯ
Ȋȱȱȱȱȱȱǰȱȱ
ǰȱĚȱȦȱȱǯȱȱ¢ȱ
ȱȱȱȱȱȱ¢ȱȱȱȱȱȱ¢
ǯ
2.
ȱ ȱȱȱǯ
3.
ȱȱȱȱȱȱȱȱǯ
4.
ȱ ȱȱȱǯ
ȱ¢ȱǰȱ£ǰȱǰȱȱȱȱȬȱ
ǯ
Note:
ȱ¢ȱȱȱȱȱȱ£ȱȱǰȱȱ
ȱȱȱȱȱȱǯ
Ȋ ȱȱȱȱȱǰȱȱȱȃȱȱȱȃȱ
ȱŗřǯ
Ȋ ȱȱȱȱȱǰȱȱȱȃȱȱǻŗ ŖŖȱ
¢ǼȃȱȱȱŗŚǯ
Ȋ ȱȱȱȱȱȱǰȱȱȱȃȱȱȱ
ȃȱȱȱŗ ǯ
Ȋ ȱȱȱȱȱǰȱȱȱȃȱȱȱȃȱ
ȱŗŜǯ
Ȋ ȱȱȱȱǰȱȱȱȃȱȃȱȱȱŗŝǯ
12
iBright

Imaging Systems User Guide

Chemi Blots
ȱȱȱȱȱǯ
1
1.
ȱȱ¡ǯ
ȱȱȱȱȱȱȱ¡ǰȱȱȱȱȱ
ȱȱȱ¡ȱǯȱȱȱȱȱȱǯ
2.
ȱȱȱȱǯȱȱȱȱȱȱ
ȱ¡ȱȱȱęȱȱȱȱǯ
3.
ǻǼȱȱȱ¡ȱȱȱȱȱDZ
Ȋ ȱ ȱȱ ǯ
Ȋ ȱȱȱȱȱǯ
Ȋ ȱȱȱȱ¡ȱȱȱȱȱ¡ȱȱȱȱȱ
¢ȱȱȱǯ
ȱȱȱȱȱȬǯ
4.
ȱȱǻȱȱȱȱǼǯ
5.
ȱȱȱȱȱǯ
ȱȱȱȱ¢ȱȱ¢ȱȱȱȱ¢ǯ
6.
ȱȱȱǰȱȱ¡ǰȱ¢ǰȱȱ¢£ǯȱȱȱ
ǰȱȱȱȱȱ£ȱȱȱȱȱȱȱȱ
ǰȱȱȱȱȱŘȱȱȱ¡ȱǯ
Image using
Chemi Blots
Operate the iBrightimager
Chemi Blots
iBright
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Imaging Systems User Guide
13

Fluorescent Blots (FL1500 model only)
ȱȱȱĚȱȱȱȱȱȱȱȱȱȱŚȱ
ȱ¡ȱȱǻǼȱȱ¡¢ȱŚ ȬŚŞ ȱǰȱ ŗ Ȭ Ś ȱǰ
ŜŖŞȬŜřŘȱǰȱŜŗŖȬŜŜŖȱǰȱȱŝŚ ȬŝŜ ȱDzȱ¡ȱȱ
ǻǼȱ ŖŞȬ ŝȱǰȱ ŜŞȬŜŗŝȱǰȱŜŝ ȬŝŘŖȱǰȱŝŗŖȬŝřŖȱȱȱŞŖŖȬŞ Ŗȱǯ
1
1.
ȱȱȱ¢ȱȱȱȱȱȱȱ¢ȱȱȱȬȱǯ
¢ǰȱȱȱȱ¢ȱȱȱȱȱȱȬȱǰȱȱ
¢ȱȱȱĴȱȱȱȱȱȱǯȱȱȱȱ¢ȱȱ
¢ȱȱȱȱȱȱȱȱȱȱ¢DZȱ¢ȱǰ
ȱǰȱ¡ȱǰȱȱȱǯ
a.
ȱȱȱ¡ȱ¢ǰȱȱȱ¢ȱȱĴǯȱȱ¢ȱȱ
ȱȱ¢ȱȱ¢ȱȱȬȱ¢ȱȱȱĚȱ¢ȱǯ
¢ȱȱȱȬȱ¢ȱȱȱ£ȱ¢ȱȱ¢ȱǰ
ǰȱȱȱǯ
b.
ȱȱȱȱȱȱȱȱȱȱȱ¢ǯ
Note:
ȱ¢ȱȱȱȱȱȱȱȱȱȱȱȱ¢ǯ
c.
ȱȱȱȱ¢ȱȱȱǰȱȱȱ¢ȱȱ
Ĵȱȱȱȱȱȱǯȱȱęǯ
2.
ȱǯ
3.
ȱȱ¡ǯȱ¢ǰȱȱȱ¡ǰȱȱ
ȱ¡ǯȱȱȱ¡ǰȱȱȱȱ¢ȱȱȱ
ȱȱȱ¢ȱǯ
ȱȱȱȱȱȱȱȱ¡ȱȱȱ¢ȱ¢ȱǯȱ
ȱȱȱȱȱȱȱȱ¡ȱȱȱ
ǯȱȱȱȱȱǯ
4.
ȱȱȱȱǯȱȱȱȱȱȱ
ȱ¡ȱȱȱęȱȱȱȱǯȱȱȱȱěǰȱǰ
ȱȱȱȱȱȱȱǯ
5.
ȱȱȱȱȱȱ¡ȱȱȱ¢ȱȱȱȱ¢ȱ
ǰȱȱȱȱǻ¢Ǽȱȱ¢ȱȱȱDZ
Ȋ ȱ ȱȱ ǯ
Ȋ ȱȱȱȱȱǯ
Ȋ ȱȱȱȱ¡ȱȱȱȱȱ¡ȱȱȱȱȱ
¢ȱȱȱǯ
ȱȱȱȱȱȬǯ
6.
ȱȱȱȱȱǯ
ȱȱȱȱ¢ȱȱ¢ȱȱȱȱ¢ǯ
7.
ȱȱȱǰȱȱ¡ǰȱ¢ǰȱȱ¢£ǯȱȱȱ
ǰȱȱȱȱȱ£ȱȱȱȱȱȱȱȱ
ǰȱȱȱȱȱŘȱȱȱ¡ȱǯ
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14
iBright
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Imaging Systems User Guide

Nucleic Acid Gels
ȱȱȱȱȱȱȱȱȱĚȱȱȱȱ
ȱȱǰȱȜȱǰȱȜȱȱǻȱȱǼǰȱȜȱȱȱ
ǯ
1
1.
ȱȱ¡ǯȱ¢ǰȱȱȱȱȱ¡ȱǰȱȱ
ȱȱȱǯ
ȱȱȱȱȱȱȱ¡ȱȱȱȱȱ
ȱȱȱȱ¡ȱǯȱȱȱȱȱȱǯ
2.
ȱȱȱȱǯȱȱȱȱȱȱ
ȱ¡ȱȱȱęȱȱȱȱǯ
3.
ȱȱȱȱȱȱ¡ȱǰȱȱȱDZ
Ȋ ȱ ȱȱ ȱȱǯ
Ȋ ȱȱȱȱȱǯ
Ȋ ȱȱȱȱ¡ȱȱȱȱȱ¡ȱȱȱȱȱ
¢ȱȱȱǯ
ȱȱȱȱȱȱȬǯ
4.
ȱȱȱȱȱȱȱȱ¡ȱǯ
ȱȱȱȱȱȱ¢ȱȱȱȱ¢ǯ
5.
ȱȱȱǰȱȱ¡ǰȱ¢ȱȱ¢£ǯȱȱȱ
ǰȱȱȱȱȱȱ£ȱȱȱȱȱ
ȱǰȱȱȱȱȱŘȱȱȱ¡ȱǯ
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Nucleic Acid Gel
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Nucleic Acid Gels
iBright
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Imaging Systems User Guide
15

Protein Gels
ȊȱȱDZȱȱȱȱȱȱ
ȱȱȱȱȱȬȱ
ȱȱȬȱȱǯ
ȊȱȱDZȱȱȱȱ
ȱȱȱȱȱȬȜȬȱ
ȱȱȜ¢Ȭȱȱǯ
1
1.
ȱȱ¢ȱȱȱȱȱȱǻȱȱǼȱ
ĚȱȱǻȱȱǼǯ
2.
ȱȬ£ȱȱȱ ȱȱȱȱȱȱȱ¢ȱȱ
ǯ
IMPORTANT!
ȱȱȱȱȱȱȱȱǰ
ȱȱȱȱȱȱȱǯ
3.
ȱȱ¡ǯȱ¢ǰȱȱȱȱȱ¡ȱǰȱȱ
ȱȱȱǯ
ȱȱȱȱȱȱȱ¡ȱȱȱȱȱ
ȱȱȱȱ¡ȱǯȱȱȱȱȱȱǯ
4.
ȱȱȱȱǯȱȱȱȱȱȱ
ȱ¡ȱȱȱęȱȱȱȱǯȱȱȱȱěǰȱǰ
ȱȱȱȱȱȱȱǯ
5.
ȱȱȱȱȱȱ¡ȱǰȱȱȱDZ
Ȋ ȱ ȱȱ ȱȱǯ
Ȋ ȱȱȱȱȱǯ
Ȋ ȱȱȱȱ¡ȱȱȱȱȱ¡ȱȱȱȱȱ
¢ȱȱȱǯ
ȱȱȱȱȱȱȬǯ
6.
ȱȱȱȱȱȱȱȱ¡ȱǯ
ȱȱȱȱȱȱ¢ȱȱȱȱ¢ǯ
7.
ȱȱȱǰȱȱ¡ǰȱ¢ȱȱ¢£ǯȱȱȱ
ǰȱȱȱȱȱȱ£ȱȱȱȱȱ
ȱǰȱȱȱȱȱŘȱȱȱ¡ȱǯ
Image using
Protein Gels
Operate the iBrightimager
Protein Gels
16
iBright

Imaging Systems User Guide

Universal Mode
ȱȱȱȱȱȱȱȱȱ¢ȱǻǰȱĚǰȱȦ
Ǽǯ
T
Table 3 Illumination sources, excitation filters, and emission filters for the iBright

CL1500 Instrument.
Illumination Excitation Filters Emission Filters
White Epi-LED 455-485 nm
400-700 nm
568-617 nm
ND filter [1] 400-700 nm
Green trans 490-520 nm
[1] Neutral density filter decreases the intensity of the white Epi-LED for visible imaging.
Table 4 Illumination sources, excitation filters, and emission filters for the iBright

FL1500 Instrument.
Illumination Excitation Filters Emission Filters
White Epi-LED
455-485 nm
400-700 nm
508-557 nm
568-617 nm
675-720 nm
710-730 nm
800-850 nm
515-545 nm
608-632 nm
610-660 nm
nIR Epi-LED 745-765 nm
ND filter 400-700 nm
Green trans 490-520 nm
1.
ȱȱȱȱȱȱȱȱȱěȱ¢ȱȱȱ
ȱȬȱǯ
¢ǰȱȱȱȱ¢ȱȱȱȱȱȱȬȱǰȱȱ
¢ȱȱȱĴȱȱȱȱȱȱǯȱȱȱȱ¢ȱȱ
¢ȱȱȱȱȱȱȱȱȱȱ¢DZȱ¢ȱǰ
ȱǰȱ¡ȱǰȱȱȱǯ
a.
ȱȱȱ¡ȱ¢ǰȱȱȱ¢ȱȱĴǯȱȱ¢ȱȱ
ȱȱ¢ȱȱ¢ȱȱȬȱ¢ȱȱȱĚȱ¢ȱǯ
¢ȱȱȱȬȱ¢ȱȱȱ£ȱ¢ȱȱ¢ȱǰ
ǰȱȱȱǯ
b.
ȱȱȱȱȱȱȱȱȱȱȱ¢ǯ
Note:
ȱ¢ȱȱȱȱȱȱȱȱȱȱȱȱ¢ǯ
c.
ȱȱȱȱ¢ȱȱȱǰȱȱȱ¢ȱȱ
Ĵȱȱȱȱȱȱǯȱȱęǯ
Operate the iBrightimager
Universal Mode
iBright

Imaging Systems User Guide
17

ȱȱȱȱ¢ȱȱȱ¢ȱȱȱȱȱȱ
ȱ¢ȱȱȱǯ
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ȊȱǽǾDZȱȱȱȱĚȱȱȱȱ¡ȱ¢
ȱȱȱȱȱȱęǯ
ȊȱDZȱȱȱȱĚȱȱȱȱȱ
¡ȱ¢ȱȱȱȱȱȱ ŜŞȬŜŗŝȱȱęȱǻ
ȱȱǼǯ
ȊDZȱȱȱȱĚȱȱȱȱȱȱȱ
¡ȱȱȱ¢ȱȱȱȱȱȱ ŜŞȬŜŗŝ
ȱęȱȯȱȱȱȱǯ
ȊDZȱȱȱȱȱȱȱȱȱ
ǻȱǰȱȱǰȱǯǼȱȱȱȬȱ
ȱȱ¢ȱęȱȱȱȱęǯ
ȊDZȱȱȱ£ȱȱȱȱ£ȱȱ
ȱȱȱȱȱ¢ȱǯ
2
2.
ȱȱ¡ǯȱ¢ǰȱȱȱ¡ǰȱȱ
ȱ¡ǯȱȱȱȱȱȱȱȱȱ¡
ȱȱ¢ȱ¢ȱǯȱȱȱȱȱȱȱȱ
ȱ¡ȱȱȱȱǯȱȱȱȱȱǯ
3.
ȱȱȱȱǯȱȱȱȱȱȱ
ȱ¡ȱȱȱęȱȱȱȱǯ
4.
ȱȱȱȱȱȱ¡ȱȱȱ¢ȱȱȱȱȱ¢ȱ
ǰȱȱȱȱǻ¢Ǽȱȱ¢ȱȱȱDZ
Ȋ ȱ ȱȱ ȱȱǯ
Ȋ ȱȱȱȱȱǯ
Ȋ ȱȱȱȱ¡ȱȱȱȱȱ¡ȱȱȱȱȱ
¢ȱȱȱǯ
Note:
ȱȱ¡ȱȱȱǰȱȱȱȱ
ȱȱȱǯ
5.
ȱȱȱȱȱȱȱȱȱȱȱȱ¡
ǯȱȱǻǼȱȱȱȱȱ¢ȱȱȱ
¢ǯ
6.
ȱȱȱǰȱȱ¡ǰȱ¢ǰȱȱ¢£ǯȱȱȱ
ǰȱȱȱȱȱȱ£ȱȱȱȱȱ
ȱǰȱȱȱȱȱřȬ ȱȱȱ¡ȱǯ
Operate the iBrightimager
Universal Mode
18
iBright

Imaging Systems User Guide

Options available in modes
ȱȱěȱȱȱȱȱĚȱȱȱȱ
ȱȱȱǯ
T
Table 5
Camera
Suboption Detail
Resolution/Sensitivity Changes binning setting to increase resolution/decrease
sensitivity or decrease resolution/increase sensitivity.
Zoom/Focus
Changes zoom level to increase or decrease image area.
Optimizes focus for sharpness.
Note:
Optical zoom 1X to 2X and digital zoom 2X to 8X.
Sample rotation
Imager mechanically rotates sample ±10° depending on
orientation. Rotation is automatic, but also allows ability to
adjust in More OptionsCamera Settings.
Table 6
Routines
Suboption Detail
Multi-exposure Series of 5 preset exposure times producing one image for
each time period.
Signal accumulation
Series of exposures where user defines the first capture
(time interval), the last capture (cumulative capture time)
and the total number of captures.
Note:
Images acquired using Signal Accumulation are not
recommended for publication or analysis. This feature is
recommended for determining an optimal exposure time.
Table 7
Image adjust
Suboption Detail
Contrast
Grayscale differentiation between image features. Can use
Auto contrast (high, medium, low) or view raw image with
no contrast. User can manually adjust Gamma, White,
Black using slider bars.
Channels and Layers
Allow user to navigate between individual channels
associated with a common image file; pertains only to
Chemi Blot, Fluorescent Blot, and Universal modes (e.g.,
Channels: membrane, Alexa Fluor488, Alexa Fluor555,
Composite). Layers allow toggling on and off analysis tools.
Operate the iBrightimager
Options available in modes
iBright

Imaging Systems User Guide
19

Suboption Detail
Zoom Increases or decreases digital zoom to select an area of the
image.
Invert Produces a negative or positive (white to black or black to
white) of the displayed image.
Saturation False-colors saturated white pixels (65,536) as red to
differentiate from non-saturated pixels.
T
Table 8 Other options
Suboption Detail
Region of Interest
Specify an area on the image to determine the optimal
exposure time for that region using the Smart Exposure
tool.
Manual exposure Manually select an exposure time for sample.
Smart exposure
Predicts optimal exposure for minimizing pixel saturation
and maximizing dynamic range for a specific sample.
Renders a preview of how the image will appear at the
recommended exposure time.
Band excision (Nucleic Acid
Gels mode)
Opens drawer and turns on green transilluminator to allow
for safe band excision on nucleic acid gels.
Membrane overlay (Chemi
and Fluorescent Blot
modes)
Allows visible membrane overlay to see any visible
prestained markers, which aid in identifying molecular
weights for unknown samples.
2UP view (Fluorescent Blot
mode)
After a Smart Exposure or Capture, images are displayed
by default in a 2UP view.
• Top image displays individual channels in grayscale.
Switch between individual channels by touching the
channel you want to display and edit. After a dye is
selected, that channel image is displayed in the top
view and becomes editable so the user can increase or
decrease the exposure time dial and see in real time
the effect on the image preview.
• Bottom image displays the color composite with
channels displayed as different false colors overlaid
on a black background. Individual channels can be
toggled on and off in the composite image by touching
at the left of the appropriate channel name.
• For the Visible channel color composite, the signal is
falseϣcolored overlaid on a white background.
Example: This channel is used to display
Coomassieϣstained protein gels.
Operate the iBrightimager
Options available in modes
20
iBright

Imaging Systems User Guide