Thermo Scientific Multifuge X1 User manual

Thermo Scientific
Multifuge X1
Multifuge X1R
Instructions for Use
50120964-g• 06/ 2020
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WEEE Conformity
This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked by the following symbol:

Thermo Scientific Multifuge X1 / X1R i
T
Preface ....................................................................................................................................... iii
Scope of Supply ....................................................................................................................... iii
Intended Use ............................................................................................................................. iii
Accident Prevention .................................................................................................................iv
Precautions ................................................................................................................................iv
Chapter 1 Introduction and Description ............................................................................................... 1-1
Characteristics of the Multifuge X1 / X1R .............................................................. 1-2
Technical Data ........................................................................................................ 1-3
Directives, Standards and Guidelines ........................................................................... 1-4
Functions and Features ............................................................................................................ 1-5
Mains Supply ........................................................................................................... 1-5
Rotor Selection ........................................................................................................ 1-6
Chapter 2 Before use ............................................................................................................................... 2-1
Before Setting up ..................................................................................................... 2-2
Transporting the Centrifuge .................................................................................... 2-2
Location .................................................................................................................. 2-2
Aligning the Centrifuge ........................................................................................... 2-3
Mains Connection ................................................................................................... 2-3
Storage ..................................................................................................................... 2-4
Chapter 3 Control Panel .......................................................................................................................... 3-1
Control Panel .......................................................................................................... 3-2
Keys ......................................................................................................................... 3-2
Chapter 4 Operation ................................................................................................................................. 4-1
Switch on Centrifuge ............................................................................................... 4-2
Lid Opening ............................................................................................................ 4-2
Close Lid ................................................................................................................. 4-2
Rotor Installation ..................................................................................................... 4-2
Entering Parameters ................................................................................................. 4-4
Centrifugation ....................................................................................................... 4-10
Temperature Adaptation during Standstill ............................................................. 4-11
Short-term Centrifugation ..................................................................................... 4-11
Removing the Rotor .............................................................................................. 4-11
Aerosol-tight Rotors ............................................................................................... 4-12
Aligning the Centrifuge ......................................................................................... 4-12
Table of Contents

Contents
ii Multifuge X1 / X1R Thermo Scientific
Chapter 5 System Menu .......................................................................................................................... 5-1
System Menu .......................................................................................................... 5-2
Chapter 6 Maintenance and Care ......................................................................................................... 6-1
Cleaning Intervalls ................................................................................................... 6-2
Cleaning .................................................................................................................. 6-2
Disinfection ............................................................................................................. 6-3
Decontamination ..................................................................................................... 6-4
Autoclaving .............................................................................................................. 6-5
Service of Thermo Fisher Scientific .......................................................................... 6-5
Chapter 7 Trouble Shooting .................................................................................................................... 7-1
Mechanical Emergency Door Release ...................................................................... 7-2
Troubleshooting by User ......................................................................................... 7-3
When to contact Customer Service .......................................................................... 7-5
Chemical Compatibility Chart ...............................................................................A-1
Index ...........................................................................................................................I-1
Description ............................................................................................................. 5-3

Thermo Scientific Multifuge X1 / X1R iii
P
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Article Number Quantity Check
Centrifuge Multifuge X1 1
Power supply cable 1
50120964 Instruction manual 1
70009824 Anti-corrosion oil 1
Intended Use
•This centrifuge is an IVD accessory, and therefore subject to the Directive 98/79/EC of the
European Parliament and the Council of October 27, 1998 on in vitro diagnostic medical
devices.
•This centrifuge is a laboratory product designed to separate components by generation of Relative
Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected
in appropriate containers, either alone or after addition of reagents or other additives.
•As general-purpose centrifuge, it is designed to also run other containers filled with chemicals,
environmental samples and other non-human body samples.
•This centrifuge should be operated by trained specialists only.

P Preface
iv Multifuge X1 / X1R Thermo Scientific
Accident Prevention
Prerequisite for the safe operation of the Multifuge X1 / X1R is a work environment in compliance
with standards, directives and trade association safety regulations and proper instruction of the user.
The safety regulations contain the following basic recommendations:
•Maintain a radius of at least 30 cm around the centrifuge.
•Implementation of special measures which ensure that no one can approach the centrifuge for
longer than absolutely necessary while it is running.
•The mains plug must be freely accessible at all times. Pull out the power supply plug or disconnect
the power supply in an emergency.
Precautions
In order to ensure safe operation of the Multifuge X1 / X1R, the following general safety
regulations must be followed:
•The centrifuge should be operated by trained specialists only.
•The centrifuge is to be used for its intended use only.
•Plug the centrifuge only into sockets which have been properly grounded.
•Do not move the centrifuge while it is running.
•Do not lean on the centrifuge.
•Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher
Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes,
provided they have been approved for the speed or the RCF value of the rotor.
•Do not use rotors which show any signs of corrosion and/or cracks.
•Do not touch the mechanical components of the rotor and do not make any changes to the
mechanical components.
•Use only with rotors which have been properly installed. Follow the instructions on the Autolock
in section “Rotor Installation” on page 4-2.
•Use only with rotors which have been loaded properly. Follow the instructions given in the rotor
manual.
•Never overload the rotor. Follow the instructions given in the rotor manual.
•Never start the centrifuge when the lid is open.
•Never open the lid until the rotor has come to a complete stop and this has been confirmed in the
display.

P Preface
Thermo Scientific Multifuge X1 / X1R v
•The lid emergency release may be used in emergencies only to recover the samples from the
centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door Release” on
page 7-2).
•Never use the centrifuge if parts of its cover panels are damaged or missing.
•Do not touch the electronic components of the centrifuge or alter any electronic or mechanical
components.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Location: well-ventilated environment, set-up on a level and rigid surface with adequate
load-bearing capacity.
•Rotor installation: make sure the rotor is locked properly into place before operating the
centrifuge.
•Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts
and vessel have to be cleaned carefully.
•Always balance the samples.
Centrifuging hazardous substances:
•Do not centrifuge explosive or flammable materials or substances which could react violently with
one another.
•The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an
explosion-prone environment.
•Do not centrifuge inflammable substances.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without
suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the “Bio-safety
Manual” of the World Health Organization WHO), aerosol-tight biological seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate
disinfection measures have to be taken (see “Disinfection” on page 6-3).
•Highly corrosive substances which can cause material damage and impair the mechanical stability
of the rotor, should only be centrifuged in corresponding protective tubes.
IF A HAZARDOUS SITUATION OCCURS, TURN OFF THE POWER SUPPLY TO THE
CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY.
Intended Use
The centrifuge is to be used for separating materials of different density or particle size suspended in a
liquid.
Maximum sample density at maximum speed: 1,2-----
cm
g
-----
3
-

vi Multifuge X1 / X1R Thermo Scientific
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
P Preface

Thermo Scientific Multifuge X1 / X1R 1-1
1
Introduction and Description
Contents
•“Characteristics of the Multifuge X1 / X1R” on page 1-2
•“Technical Data” on page 1-3
•“Directives, Standards and Guidelines” on page 1-4
•“Functions and Features” on page 1-5
•“Mains Supply” on page 1-5
•“Rotor Selection” on page 1-6

1 Introduction and Description
Characteristics of the Multifuge X1 / X1R
1-2 Multifuge X1 / X1R Thermo Scientific
Characteristics of the Multifuge X1 / X1R
The Multifuge X1 / X1R is an in-vitro diagnostics device according to the In-Vitro Diagnostics
Directive 98/79/EC.
Several rotors with a wide range of tubes can be used.
The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and
low-vibration operation even at high speeds, and guarantees a very long lifetime.
The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time,
temperature, and running profile (acceleration and braking curves). You can choose between the
display of speed and RCF or the entry mode.
These settings can be changed even while the centrifuge is running.
With the help of the PULSE key , you can also centrifuge a sample for just a few seconds, if
called for.
The Multifuge X1 / X1R is equipped with various safety features:
•The housing and rotor chamber consist of steel plate, the interior of armor steel, while the front
panel is made of high-impact resistant plastic.
•The lid is equipped with a view port and a lock.
•The lid of the centrifuge can only be opened while the centrifuge is switched on and the rotor has
come to a complete stop. The centrifuge cannot be started until the lid has been closed properly.
•The integrated rotor detection systems ensures that no inadmissible speed settings can be
preselected.
•Electronic imbalance recognition
•Lid emergency release: For emergencies only, e.g. during power failures (see “Mechanical
Emergency Door Release” on page 7-2)

1 Introduction and Description
Technical Data
Thermo Scientific Multifuge X1 / X1R 1-3
Technical Data
The technical data of the Multifuge X1 / X1R is listed in the following table.
Table 1-1. Technical Data Multifuge X1 / X1R
Feature Value
Environmental conditions -Use in interior spaces
-Altitudes of up to 2,000 m above sea level
-max. relative humidity 80 % up to 31 °C; decreasing linearly up to 50 %
relative humidity at 40 °C.
permissible ambient temperature +2 °C to +35 °C +2 °C to +35 °C
Overvoltage category II II
Pollution degree 2 2
Heat dissipation refrigerated ventilated
230 V 120 V 230 V 120 V
4778 BTU/h 4096 BTU/h 3447 BTU/h 3901 BTU/h
IP 20 20
running time unlimited unlimited
max Speed nmax 15200 rpm (depending on the rotor) 15200 rpm (depending on the rotor)
min Speed nmin 300 rpm 300 rpm
maximum RCF value at nmax 25830 x g 25830 x g
maximum kinetic energy 62.5 kJ 51.7 kJ
noise level at maximum speed < 63 dB (A) < 63 dB (A)
temperature setting range -10 °C to +40 °C
Dimensions refrigerated ventilated
Height
Height with lid open
Width
Depth
Table top height
360 mm
870 mm
623 mm
605 mm
310 mm
360 mm
870 mm
440 mm
605 mm
310 mm
weight without rotor 91.5 kg 57.5 kg
Order No. Refrigerant Quantity Pressure GWP CO2e
75004250 R-134a 0.28 kg 21 bar 1430 0.40 t
Centrifuge
Thermo Scientific Multifuge X1R
Contains fluorinated greenhouse gases in a hermetically sealed system.
Refrigerant
75004251 R-134a 0.28 kg 21 bar 1430 0.40 t
Thermo Scientific Multifuge X1R
Table 1-2. Functions and Features

1 Introduction and Description
Directives, Standards and Guidelines
1-4 Multifuge X1 / X1R Thermo Scientific
Directives, Standards and Guidelines
Table 1-3. Directives, Standards and Guidelines
Region Directive Standard
98/79/EC In Vitro Diagnostics
2011/65/EU RoHS
Directive on the Restriction of the use of
certain Hazardous Substances in electrical
and electronic equipment
Protective golas of:
2006/42/EC Machinery
2014/35/EU Low Voltage
2014/30/EU Electromagnetic Compatibility
(EMC)
EN 61010-1
EN 61010-2-020
EN 61010-2-101
EN 61326-1 Class B
EN 61326-2-6
EN ISO 14971
EN ISO 13485
USA & Canada ANSI/UL 61010-1/CAN/CSA-C22.2 No. 61010-1
IEC 61010-2-020
IEC 61010-2-101
EN ISO 14971
EN ISO 13485
Emitted interference FCC Part 15 CLASS A
NOTE: This equipment has been tested and found to comply
with the limits for a Class A digital device, pursuant to Part 15
of the FCC Rules.
These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in
a commercial environment. This equipment generates, uses,
and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this
equipment in a residential area is likely to cause harmful
interference in which case the user will be required to correct
the interference at his own expense.
Japan
China
IEC 61010-1
IEC 61010-2-020
IEC 61010-2-101
IEC 61326-2-6
IEC 61326-1 Class B
EN ISO 14971
EN ISO 13485
Europe
CFDA listed
FDA listed
Product code JQC
centrifuges for clinical use
Device class 1

1 Introduction and Description
Functions and Features
Thermo Scientific Multifuge X1 / X1R 1-5
Mains Supply
Functions and Features
The following table gives an overview of the important functional and performance characteristicsof the
Multifuge X1 / X1R.
Table 1-4. Functions and Features
Component / Function Description / Features
Structure / Housing Galvanized steel chassis with armored plating
Chamber Stainless steel
Drive Induction drive without carbon brushes
Keys and display Easy-to-clean keypad and display surface
Controls Microprocessor-controlled
Internal memory The most recent data is saved
Functions RCF-selection, temperature control, pretemp with cooling equipped
devices
Acceleration / braking profiles 9 acceleration and 10 braking curves
Rotor recognition Automatic
Imbalance recognition Electronic, contingent on rotor and speed
Lid lock Automatic lid closing and locking starting from an initial hold position
The following table contains an overview of the electrical connection data for the Multifuge X1 / X1R.
This data is to be taken into consideration when selecting the mains connection socket.
Table 1-5. Electrical connection data of the Multifuge X1 / X1R
Cat. Mains
voltage Frequency Rated current
Power
consumption
Equipment
fuse
Building
fuse
75004250 refrigerated 230 V ±10 % 50 / 60 Hz 8 A 1400 W 15 AT 16 AT
75004251 refrigerated 120 V ±10 % 60 Hz 12 A 1200 W 15 AT 15 AT
75004210 ventilated 230 V ±10 % 50 / 60 Hz 6 A 1010 W 15 AT 16 AT
75004211 ventilated 120 V ±10 % 60 Hz 9.5 A 850 W 15 AT 15 AT

1 Introduction and Description
Rotor Selection
1-6 Multifuge X1 / X1R Thermo Scientific
Rotor Selection
The Multifuge X1 / X1R is supplied without a rotor.
Various rotors are available to choose from.
The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.
For more information visit our website at: http://www.thermofisher.com
TX-400 75003629
with round buckets 75003655
TX-200 75003658
with round buckets 75003659
BIOShieldTM 720 75003621
M-20 75003624
Microliter 30 x 2 sealed 75003652
CLINIConicTM 30 x 15 75003623
8 x 50 sealed 75003694
FIBERLiteTM F15-6 x 100 75003698
FIBERLiteTM F13-14 x 50c 75003661
FIBERLiteTM F15-8 x 50c 75003663
FIBERLiteTM F21-48 x 275003664
Microliter 48 x 2 sealed 75003602
HIGHConicTM II 75003620

Thermo Scientific Multifuge X1 / X1R 2-1
2
Before use
Contents
•“Before Setting up” on page 2-2
•“Transporting the Centrifuge” on page 2-2
•“Location” on page 2-2
•“Aligning the Centrifuge” on page 2-3
•“Mains Connection” on page 2-3
•“Storage” on page 2-4

2 Before use
Before Setting up
2-2 Multifuge X1 / X1R Thermo Scientific
Before Setting up
1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.
2. Remove the packaging.
3. Check the order for completeness (see “Scope of Supply” on page iii).
If the order is incomplete, please contact Thermo Fisher Scientific.
Transporting the Centrifuge
•Due to its weight (see “Technical Data” on page 1-3), the centrifuge should be carried by several
people.
•Always lift the centrifuge at both sides.
Figure 2-1. Lifting the centrifuge at both sides.
•The centrifuge can be damaged by impacts.
•Transport the centrifuge upright and if at all possible in its packaging.
Location
The centrifuge should only be operated indoors.
The set-up location must fulfil the following requirements:
•A safety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of the safety zone while centrifuging.
•The supporting structure must be stable and free of resonance, for example a level laboratory
bench.
•The supporting structure must be suitable for horizontal setup of the centrifuge.
WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front
or the back panel.
Always remove the rotor before moving the centrifuge.

2 Before use
Aligning the Centrifuge
Thermo Scientific Multifuge X1 / X1R 2-3
•The centrifuge should not be exposed to heat and strong sunlight.
•The set-up location must be well-ventilated at all times.
Aligning the Centrifuge
The horizontal alignment of the centrifuge must be checked every time after moving it to a different
location.
The supporting structure must be suitable for horizontal setup of the centrifuge.
Mains Connection
Figure 2-2. Mains Connection
1. Turn off the power supply switch on the back (press “0”).
2. Plug the centrifuge into grounded electrical sockets only.
3. Check whether the cable complies with the safety standards of your country.
4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
5. Establish the connection to the power supply with the connecting cable.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
CAUTION If the centrifuge isn’t level, imbalances can occur and the centrifuge can be
damaged.
Do not place anything under the feet to level the centrifuge.
Main Power Switch
Mains plug

2 Before use
Storage
2-4 Multifuge X1 / X1R Thermo Scientific
Storage
•Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected
and decontaminated.
•Store the centrifuge in a clean, dust-free location.
•Be sure to place the centrifuge on its feet.
•Avoid direct sunlight.

Thermo Scientific Multifuge X1 / X1R 3-1
3
Control Panel
Contents
•“Control Panel” on page 3-2
•“Keys” on page 3-2

3 Control Panel
Control Panel
3-2 Multifuge X1 / X1R Thermo Scientific
Control Panel
The control panel contains the keys and displays of the centrifuge (only the power switch is located on
the back of the device). All parameters can be selected and changed during operation.
Keys
The keys allow user intervention for controlling the operating mode as follows:
PULSE
Program selection keys OPEN
Display
Directory key SPEED START
TIME ENTER
Braking and Acceleration Key
TEMP
Changing the display mode Speed / RCF Value
numeric pad
Bucket
STOP
Changing the display mode Running time / Remaining Running time
Changing the display mode chamber / sample temperature
Key Display contents
Start
Normal start of the centrifuge
Stop
End run manually
Open lid
Automatic release (possible only when device is switched on). Emergency
release (see “Mechanical Emergency Door Release” on page 7-2)
Pulse
By pressing the key PULSE the centrifuge starts immediately and
accelerates up to the end speed. Releasing the key initiates a stopping
process at the highest braking curves.
Directory
Use the directory key in order to modify the displayed value
Bucket
Use the bucket key in order to have all available bucket types
displayed in succession.
Braking and
Acceleration Curves
Use the ACC DEC key to toggle between braking and acceleration
curves.
Changing the display
mode
Use the Toggle key to change the display mode. (speed / RCF value,
sample / chamber temperature, run time counter from start or preset
speed on)