Thermo Scientific Centra-W Cell Washer User manual

SERVICE
MANUAL
SM2392
Revision 1
Centra-W
CellWasher
Cat. No. 2392 -- For 100/120/220/240 VAC, 50/60 Hz

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Centra-W Cell Washer Service Manual
TABLE OF CONTENTS
1DESCRIPTION ................................................................................................................. 4
2INSTALLATION ................................................................................................................ 5
2.1 ReceivingInspection ................................................................................................... 5
2.2 Unpacking ................................................................................................................... 5
2.3 PowerConfiguration.................................................................................................... 7
Voltage........................................................................................................................ 7
Fuses .......................................................................................................................... 7
Power Cord ................................................................................................................. 8
2.4 TubingInstallation ....................................................................................................... 8
3 OPERATION ...................................................................................................................... 9
3.1 ControlPanel .............................................................................................................. 9
3.2 Description of Operation.............................................................................................. 11
3.3 OperationalPrecautions.............................................................................................. 13
3.4 Saline Solution - Priming and Volume Calibration ....................................................... 13
Priming........................................................................................................................ 13
VolumeCalibration ...................................................................................................... 14
3.5 Wash Mode................................................................................................................ 15
3.6 Spin Mode................................................................................................................... 16
Timed.......................................................................................................................... 16
Untimed (Hold) ............................................................................................................ 16
3.7 AgitateMode............................................................................................................... 16
3.8 OperatingProcedure ................................................................................................... 17
3.9 Anti-HumanGlobulin ................................................................................................... 18
4MAINTENANCE................................................................................................................ 19
4.1 PreventiveMaintenance.............................................................................................. 19
Daily............................................................................................................................ 19
Weekly ........................................................................................................................ 19
Three Months .............................................................................................................. 20
4.2 ReplacementParts...................................................................................................... 21
4.3 Guard Bowl Removal .................................................................................................. 21
4.4 Pump Tubing Replacement ......................................................................................... 21
4.5 Decontamination ......................................................................................................... 23
4.6 Cover Interlock Bypass ............................................................................................... 24
5WARRANTY ..................................................................................................................... 25
5.1 Condition Of Returned Equipment............................................................................... 25
6 SPECIFICATIONS.............................................................................................................. 26

2Centra-W Cell Washer Service Manual
7SERVICE .......................................................................................................................... 27
7.1 Warnings And Cautions............................................................................................... 27
7.2 General ....................................................................................................................... 27
7.3 Troubleshooting .......................................................................................................... 28
8CABINET .......................................................................................................................... 29
8.1 CabinetRemoval......................................................................................................... 29
8.2 Control Panel Removal ............................................................................................... 29
8.3 Latch Assembly........................................................................................................... 29
8.4 Hinges......................................................................................................................... 30
8.5 Cover Assembly .......................................................................................................... 31
8.6 BaseAssembly Components ...................................................................................... 31
9 POWER.............................................................................................................................. 32
9.1 Power Circuit............................................................................................................... 32
9.2 BlowingFuses............................................................................................................. 32
10 CIRCUIT BOARD.............................................................................................................. 34
10.1 General ................................................................................................................. 34
10.2 LEDs ..................................................................................................................... 34
10.3 Fuse ...................................................................................................................... 35
10.4 Test Points ............................................................................................................ 35
11 DRIVEASSEMBLY........................................................................................................... 36
11.1 General ................................................................................................................. 36
11.2 Motor..................................................................................................................... 36
11.3 Brake..................................................................................................................... 37
11.4 DecantSolenoid .................................................................................................... 37
11.5 Rotor Repair.......................................................................................................... 37
11.6 Rotor Dependant Parts.......................................................................................... 40
12 PUMP .............................................................................................................................. 41
12.1 General ................................................................................................................. 41
12.2 Pump Motor........................................................................................................... 41
12.3 Flow Switch ........................................................................................................... 42
13 DRAWINGS...................................................................................................................... 43
IMPORTANT
This manual may not contain information on all changes that have occurred to the subject instru-
ment since the manual issue date. It was prepared for use by IEC authorized factory-trained
service or dealer personnel who are kept current through a program of service letters and bulletins
and training seminars.
This manual contans WARNINGS against operating procedures which could result in an accident
and/or personal injury. It also contains CAUTIONS against procedures which could result in
damage to your centrifuge or accessory equipment. Read this manual thoroughly before operating
or servicing this centrifuge.

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Centra-W Cell Washer Service Manual
LIST OF ILLUSTRATIONS
Figure Page
2-1 Unpacking..................................................................................................5
2-2 Voltage Selection.......................................................................................7
3-1 Centra-W Wash Cycle .............................................................................11
3-2 Rotor And Plunger Installation.................................................................14
4-1 Pump Assembly.......................................................................................21
4-2 Cover Interlock Bypass............................................................................23
Chapter 13 Drawings ......................................................................................43

4Centra-W Cell Washer Service Manual
1 DESCRIPTION
The Model Centra-W Cell Washer is manufactured by the International
Equipment Company. This benchtop cell washer is used to automatically
perform the washing phase of the anti-globulin procedure in tests using up to
six drops of blood sample. The cell washer will accommodate either twelve 12
x 75 mm tubes, or twelve 10 x 75 mm tubes. It is registered as a device in
accordance with FDA regulations governing the distribution and use of such
products.
The Cell Washer incorporates a power entry module which permits you to
select operation with 100, 120, 220, 230 or 240 Vac, 50/60 Hz power.
The Centra-W cell washer contains a microprocessor-controlled centrifuge
and a peristaltic pump for dispensing saline solution.
A cabinet supports a centrifugation chamber which is a removable plastic
guard bowl. A drive assembly is mounted to the cabinet base. An induction
motor is mounted to the drive assembly providing brushless operation. The
motor has permanently-sealed self-lubricating ball bearings. The motor shaft
projects through the center of the guard bowl. A solenoid plunger and a rotor
is set on the motor shaft. A cover assembly forms the top section of the
chamber. The aluminum cover assembly consists of a feed-dispensing
nozzle for inserting saline solution into the tubes in the rotor, a cylindrical
splash guard for directing the decanted waste into the drain hole in the
chamber, a latch, and a viewport for easy speed verification.
When the cover is closed and latched, an interlock prevents the cover from
opening when the rotor is in motion. The interlock also prevents operation
when the cover is not closed and latched except in the CALIBRATE and
AGITATE mode. The remaining electrical and electronic components, along
with the saline solution peristaltic pump and the rotor drive assembly, are
located on the base section of the cabinet.

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Centra-W Cell Washer Service Manual
2 INSTALLATION
2.1 Receiving Inspection
The Cell Washer is shipped in a special carton designed to protect it from
hazards in transit. Before signing the delivery receipt and accepting the
shipment, inspect the shipping carton for any signs of mishandling, such as
broken or dented sides or SHOCKWATCH® activation. Any observed
damage must be stated, in writing, on the delivery receipt before signing the
receipt. A normal or undamaged carton does not necessarily ensure that the
contents are undamaged. If mishandling or shipping damage is suspected,
contact the office of the carrier so that a representative may be called in to
witness the unpacking. IEC is not responsible for damage incurred in transit.
2.2 Unpacking
CAUTION: The Cell Washer weighs 45 lbs. (20.4 kg). Unpacking
and installation may require two people. Do not use any
sharp instrument to open the inner carton packaging,
damage to the instrument may result.
Note: Refer to Figure 2-1 while unpacking.
1. Remove the small box containing the rotor (Figure 2-1).
2. Remove the Power Cords and fuses.
3. Remove the top cardboard supports.
4. Remove the cardboard protector from the Cell Washer.
5. Grip the cell washer under the top edge of the front panel and
under the top edge of the pump enclosure and lift it free of the
carton. (Have someone hold the carton, if necessary).
6. Place the cell washer on a sturdy, level bench near a waste
container or drain to receive decanted saline.

6Centra-W Cell Washer Service Manual
CAUTION: The bottom of the cell washer must be high enough so
that the drain hose is pitched downward from the unit to
the drain, with no restriction to gravity flow. The drain
hose may not be extended or constricted. Reduction of
the drain flow can cause saline back up resulting in
seriouscorrosion damage.
Allow a minimum of 12 cm (5 in.) of clearance on all
sides of the cell washer for proper air cooling
circulation.
Do not plug in the power cord. The Cell Washer should
not be turned on at this time.
7. Carefully inspect the cell washer
for external damage. Any
damage discovered which is
attributable to mishandling or
shipping should be documented,
and a signed inspection report
should be furnished to the
shipping company.
8. Complete the warranty
registration card and return it to
IEC.
Figure 2-1 Unpacking

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Centra-W Cell Washer Service Manual
Voltage
Fuse
Cat No.
100V/120V
250V 5A T
49946
220V/240V
250V 2.5A T
49937
2.3 Power Configuration
The Cell Washer uses AC power at 100,120, 220, 230 or 240 volts, at 50 or
60 Hz. The unit is shipped without fuses installed and must be configured for
power at your site. Do not plug in the cell washer until you have
configured it correctly. Variations in line voltage or frequency will affect the
unit’s speed and other characteristics.
Caution: configuring the cell washer incorrectly will void your warranty.
Voltage Note: Refer to figure 2-2 while performing this procedure.
Locate the power entry module on the rear of the unit (Figure 2-2). On the
right side of this module is the fuse drawer. A small latch on the left holds the
drawer in place. Press the latch and slide the drawer out. When removing the
fuse drawer, be careful that the fuses do not fall out.
If the number visible in the window differs from the voltage at your site,
remove the square insert, rotate it, and reinstall it so that the correct voltage is
displayed through the window and insert appropriate fuse value (see chart
below).
Note: Use 240V fuse drawer position for 230V service.
CAUTION: This instrument must be configured for the correct line
voltage and must have correct fuse values. Incorrect
settings may cause fuses to blow and/or cause damage
to electrical controls.
Fuses From the following table, determine the correct fuses. Insert two fuses in the
fuse drawer and replace the fuse drawer in the power entry module. Make
sure it locks into place.
220V/230V/240V

8Centra-W Cell Washer Service Manual
Figure 2-2 Voltage Selection
Power Cord The unit requires a grounded power supply (3-prong receptacle). If your
facility does not have properly grounded power outlets, arrange for proper
grounding. Do not remove the power cord plug to attach a power plug that
does not have a grounding pin.
WARNING: Grounding - this instrument is equipped with a three
prong plug for safety purposes. The wall outlet must be
a three wire receptacle (one being GROUND). If the
outlet is not, then contact an electrician for wiring
changes.
The unit is provided with two power cords. One is suitable for North America
and Japan. The other is a “Continental Europe” type. Attach the appropriate
power cord to the power entry module receptacle on the Cell Washer. Do not
connect to AC outlet at this time.
2.4 Tubing Installation
The cell washer is shipped with the pump tubing already installed in the
peristaltic pump at the rear of the cell washer. The saline supply tubing
(including volume control flow valve and supply adapter) is also connected
(by a 90° elbow) to the pump tubing which extends from the pump. Connect
the saline supply tubing to a saline solution supply container using the
adapter.
NOTE: The cell washer has a gravity drain. Therefore be sure the drain
tubing flows downward and that it is not restricted in any way.
For best results, locate the cell washer next to a sink for
draining purposes. Periodically inspect the drain tubing to
ensure its proper operation. If the drain line becomes kinked or
restricted, drained liquid can back up the drain hose and into
the motor compartment causing motor failure.

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Centra-W Cell Washer Service Manual
3 OPERATION
3.1 Control Panel
The membrane control panel contains switches which are actuated by
pressure. The switches will sound (beep) when pressed. Indicators light when
corresponding keys are pressed.
Power Switch The Power Switch is located at the front lower-right of the cabinet. This
On-Off rocker switch is used to control the main power coming from the line
cord at the rear of the cabinet. When the Power Switch is pushed to the ON
position the STOP and the OPEN COVER light emitting diode (LED)
indicators on the control panel will light and zeroes will appear in the TIME
and CYCLE digital displays.
Run This key is used to start the wash or spin run. The indicator to the left of
this key will light when the RUN key is pressed, indicating that the instrument
is in the run mode.
Stop This key may be used at any time and in any mode to stop a run. The
indicator to the left of this key will light when the stop key is pressed,
indicating that the unit is in the brake mode.
Note: No changes can be made during a run. All keys are disabled except
for the STOP and CHECK keys.
Check This key is used to temporarily interrupt a wash cycle at the end of its current
phase (i.e. at the end of fill, spin, decant, or agitate). The cover may be
opened (by pressing the OPEN COVER key once the OPEN COVER LED
lights) for access to the rotor. Closing the cover and pressing the RUN key
will resume the wash cycle run where it was interrupted. The check function
can only be used in the wash mode. The indicator to the left of this key lights
when the CHECK key is pressed to signal that the unit is in the check mode.
Open Cover This key is used to power the cover solenoid and open the cover. The
indicator to the left of this key will light whenever the Power Switch is in the
On position and the rotor is stopped. Press the OPEN COVER key to release
the cover.