Thermo Scientific 3960 Operating instructions

Model 3960 Series
29 cu ft Environmental Chamber
Operating and Maintenance Manual 7003960 Rev. 15
Visit us online to register your warranty
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Thermo Scientific
MANUAL NUMBER 7003960
15 29318/IN-4272 12/14/12 Clarified usage on 3960-06-1 exploded drawing list ccs
14 24921/IN-3939 2/16/12 Removed accessory list - 7-2 ccs
13 27292/IN-4073 9/29/11 Revised drawings on pgs 8-3 and 8-4, spacers and power supply kit ccs
12 25454/IN-3930 4/16/09 Corrected figures on page 1-1 to 3960 ccs
11 24809/IN-3872 10/10/08 Updated temp probe part number to 290138 ccs
10 -- 8/27/07 Removed typo ‘connected to gas supply’ in Start-Up ccs
9 23572/IN-3320 10/03/06 Updated seal on blower motor mount (drawing 1900014-06-1r2) ccs
-- -- 12/2/04 Clarified operating temperature setpoint range ccs
-- -- 1/19/04 Clarified overtemp operation aks
8 21980/IN-3156 11/7/03 Added min/max load and analog output board max external cable length ccs
Preface
29 cu ft Environmental Chamber i
*All units are 50/60 Hz.
Models covered by this manual
Model Voltage*
3960 100-120
3961 200-230
Warning If the chamber is not used in the manner specified is in this
operating manual, the protection provided by the equipment design may
be impaired. s

Thermo Scientificii 29 cu ft Environmental Chamber
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related
to the use of this manual.
©1999 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific 29 cu ft Environmental Chamber iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv 29 cu ft Environmental Chamber
Preface
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29 cu ft Environmental Chamber vThermo Scientific
Table of Contents
Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Install the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Preliminary Cleaning and Disinfecting . . . . . . . . . . . . . . . . . . . . . .1-4
Install the Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Level the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Connect to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Set the Operating Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Set the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrate the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Temperature Stabilization Periods . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Turn All Audible Alarms On/Off . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set Low Temp Alarm Limit (Tracking Alarm) . . . . . . . . . . . . . . . . . .3-2
Enable Temp Alarms to Trip Relay Contacts . . . . . . . . . . . . . . . . . . .3-2
Communications Address - RS485 . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Clean the Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Replace the Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Discarding/ Removing Incubator from Service . . . . . . . . . . . . . . . . . .5-3
Section 1
Section 2
Section 3
Section 4
Section 5

vi 29 cu ft Environmental Chamber Thermo Scientific
Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Connect the Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Connect the RS485 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Connect the Analog Output Boards . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Inner Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4
Shaker Support Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Table of Contents
Section 6
Section 8
Section 7
Section 9
Appendix A

29 cu ft Environmental Chamber 1-1Thermo Scientific
Section 1 Installation and Start-Up
Figure 1-1. Front View
Figure 1-4. Side View
Figure 1-3. Back View
Figure 1-2. Top View

1-2 29 cu ft Environmental Chamber Thermo Scientific
Section 1
Installation and Start-Up
Silence - Silences the audible alarm.
Alarm Indicator - Light pulses on/off during an alarm condition in
the unit.
Mode Select Switch - Used to select Run, Setpoints, Calibration and
System Configuration Modes.
Message Center - Displays system status.
Mode Select Indicators -
• Run: Run Menu
• Set: Set Points Menu
• Cal: Calibrate Menu
• Config:: Configuration Menu
Up and Down Arrows - Increases or decreases the number values,
toggles between choices.
Enter - Stores the value into computer memory.
Heat Indicator - Lights when power is applied to the heaters.
Temp Display – Displays temperature continuously
Scroll for Parameters Arrows - Moves the operator through the choices
of the selected mode.
Control Panel Keys,
Displays & Indicators
Silence Mode System OK 36.9
Enter
Run Set Cal Config
Heat Temp
Scroll for Parameters
Silence
Alarm Indicator
Mode Select
Switch
Message
Center
Mode Select
Indicators
Up and Down
Arrows
Enter
Heat Indicator
Temp Display
Scroll for Parameters
Figure 1-5. Control Panel

29 cu ft Environmental Chamber 1-3Thermo Scientific
Section 1
Installation and Start-Up
Install the Unit
The Model 3960 Series Reach-In Incubator has four basic modes that
allow incubator setup: Run, Setpoints, Calibration and System
Configuration.
• Run is the default mode during normal operation.
• Set is used to enter system setpoints.
• Calibration is used to calibrate various system parameters.
• Configuration allows for custom setup of various options.
The chart below shows the selections under each of the modes.
Scroll for Parameters Arrows: Steps the operator through the parameters
of SET, CAL and CONFIG Modes. The right arrow goes to the next
parameter, the left arrow returns to the previous parameter.
Up Arrow: Increases or toggles the parameter values that have been select-
ed in the SET, CAL, and CONFIG Modes.
Enter: Must press Enter key to save to memory all changed values.
Down Arrow: Decreases or toggles the parameter values that have been
selected in the SET, CAL and CONFIG Modes.
Silence Key: Press to silence the audible alarm. See Section 4 for alarm
ringback times.
Units must be installed against a wall or similar structure. Maintain a three
inch clearance behind the incubator for electrical connections.
Locate the unit on a firm level surface capable of supporting the unit’s
weight of approximately 500 lbs. Position it away from doors and windows
and heating and air conditioning ducts.
Warning This incubator weighs approximately 500 lbs. Have sufficient
personnel available when moving. s
Keypad Operation
RUN SETPOINT CALIBRATION CONFIGURATION
Default Mode Temperature Temp Offset Audible
Overtemp Access Code
Temp Lo Alarm
Temp Relay
RS485 Address

Disinfect all interior surfaces with a general-use laboratory disinfectant.
Rinse thoroughly with sterile distilled water, then 70% ethanol. Dry with a
sterile cloth as needed.
Disinfect the shelf channels and shelves, then rinse with distilled water
before installing.
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment. s
Caution Accidental spills of hazardous materials on or inside this unit are
the responsibility of the user. s
The shelves may be installed at any level in the incubator. Install a shelf
channel on each side. With the tabs pointing up, attach the channel by
locating the rivet into a slotted hole, far end first. Pull the channel toward
the front and slide the front rivet on the channel into the slotted hole and
press down. Make sure that the channels are opposite each other so that
the installed shelf will be level.
Place a bubble-type level on a shelf inside the incubator. Adjust the feet as
needed; counterclockwise to lengthen or clockwise to shorten. Level the
unit front-to-back and left-to-right.
1-4 29 cu ft Environmental Chamber Thermo Scientific
Section 1
Installation and Start-Up
Preliminary Cleaning and
Disinfecting
Install the Shelves
Figure 1-5. Shelf Channel on Side Duct
Level the Unit

See the serial tag on the side of the unit for electrical specifications or refer
to the electrical schematics at the end of this manual.
Warning Connect the incubator to a grounded, dedicated circuit. The
power cord connector is the mains disconnect device for the incubator.
Position the incubator so the unit can be easily disconnected. s
Plug the provided power cord into the power inlet connector on the back
of the cabinet, then into the grounded, dedicated electrical circuit.
The Model 3960 Series also has an internal outlet located on the right side
of the interior back wall. The outlet is to provide power (230W
maximum) to accessory equipment. This outlet is not to be used when the
temperature is above 40°C.
With the incubator properly installed and connected to power, system
setpoints can be entered. The following setpoints can be entered in Set
Mode: Temperature and Overtemperature. To enter Set Mode, press the
Mode key until the Set indicator lights. Press the right and/or left arrow
keys until the proper parameter appears in the message display center. See
Chart 1-1 for more detail.
This incubator has an operating temperature setpoint range of 5.0°C above
ambient to 60.0°C. It is shipped from the factory with a temperature
setpoint of 10.0°C. At this setting, all heaters are turned off. To change the
operating temperature setpoint:
1. Press the Mode key until the Set indicator lights.
2. Press the right arrow until “Temp XX.X” is displayed in the message
center.
3. Press up/down until the desired temperature setpoint is displayed.
4. Press Enter to save the setpoint.
5. Press the Mode key until the Run indicator lights for Run mode or
press the right/left arrow keys to go to next/previous parameter.
Caution Any equipment placed inside chamber must be rated for unit
operating temperature. s
29 cu ft Environmental Chamber 1-5Thermo Scientific
Section 1
Installation and Start-Up
Connect to Electrical
Power
Incubator Start-Up
Set the Operating
Temperature

Caution The independent overtemp system is designed as a safety to
protect the incubator only. It is not intended to protect or limit the
maximum temperature of the cell cultures or customer’s equipment inside
the incubator if an overtemp condition occurs. s
The incubator is equipped with an independent circuit that monitors the
air temperature in the cabinet. The independent overtemp circuit is
designed as a safety for the incubator only. Should the system’s
temperature control fail, this circuit would cut out all heaters when the
cabinet’s temperature reaches the Overtemp setpoint. When an incubator
is operating in an overtemp condition, the temperature control in the
incubator will be ±1°C around the overtemp setpoint.
The overtemp setpoint is set by the factory (default) at 40°C. However, the
overtemp can be reset over a range from 0.5°C above the operating
temperature setpoint to 65°C.
If the incubator’s operating temperature setpoint is set above the overtemp
setpoint, the overtemp setpoint will automatically update to 1°C above the
temperature setpoint. It is recommended that the overtemp setpoint be
maintained at 1°C over the operating temperature setpoint.
To set the Overtemp setpoint:
1. Press the Mode key until the Set indicator lights.
2. Press the right arrow until Otemp XX.X is displayed in the message
center.
3. Press the up or down arrow until the desired Otemp setpoint is
displayed.
4. Press Enter to save the setting.
5. Press the Mode key until the Run indicator lights, or press the right or
left arrow to go to the next or previous parameter.
1-6 29 cu ft Environmental Chamber Thermo Scientific
Section 1
Installation and Start-Up
Set the Overtemp Setpoint

29 cu ft Environmental Chamber 1-7Thermo Scientific
Section 1
Installation and Start-Up

29 cu ft Environmental Chamber 2-1Thermo Scientific
Section 2 Calibration
After the unit has stabilized, several different systems can be calibrated. In
the calibration mode, the air temperature, CO2 and RH levels can be
calibrated to reference instruments. To access the calibration mode, press
the Mode key until the Cal indicator lights. Press the right and/or left
arrow until the proper parameter appears in the message center. See Chart
2-1 at the end of this section for more detail.
Calibration frequency is dependent on use, ambient conditions and
accuracy required. A good laboratory practice would require at least an
annual calibration check. On new installations, all parameters should be
checked after the stabilization period.
Prior to calibration, the user should be aware of the following system
functions. While the unit is in Calibration mode, all system control
functions are stopped so the unit remains stable. Readout of the system
being calibrated will appear on the message center. If no keys are pressed
for approximately five minutes while in Calibration mode, the system will
reset to Run mode so control functions are reactivated.
Caution Before making any calibration or adjustments to the unit, it is
imperative that all reference instruments be properly calibrated. s
Before calibration, allow the cabinet temperature to stabilize. Place the
calibrated instrument in the center of the chamber. The instrument should
be in the airflow, not against the shelf.
Calibrate the
Temperature

2-2 29 cu ft Environmental Chamber Thermo Scientific
Section 2
Calibration
Start-Up - Allow 12 hours for the temperature in the cabinet to stabilize
before proceeding.
Presently Operating - Allow at least 2 hours after the display reaches set-
point for temperature to stabilize before proceeding.
1. Press the Mode key until Cal indicator lights.
2. Press the right arrow until “TEMPCAL XX.X” appears in the message
center.
3. Press the up/down arrow to match the display to the calibrated
instrument.
4. Press Enter to store calibration.
5. Press the Mode key to return to Run or the right/left arrow to go to
next/previous parameter.
Temperature Stabilization
Periods

29 cu ft Environmental Chamber 2-3Thermo Scientific
Section 2
Calibration

29 cu ft Environmental Chamber 3-1Thermo Scientific
Section 3 Configuration
Several features available in the Configuration Mode allow custom setup of
the incubator. These features are listed and described below. All features
may not be necessary in all applications, but are available if needed. To
enter Configuration mode, press the Mode key until the Config indicator
lights. Press the right and/or left arrow until the appropriate parameter
appears in the message center. See Chart 3-1 at the end of this section for
more detail.
The audible alarms can be turned on or off. The factory setting is ON.
1. Press the Mode key until the Config indicator lights.
2. Press the right arrow until Audible XXX is displayed in the message
center.
3. Press up/down arrow to toggle Audible ON/OFF.
4. Press Enter to save the setting.
5. Press the Mode key to return to run mode or right/left to go to
next/previous parameter.
A 3-digit Access Code can be entered to avoid unauthorized personnel
from changing the setpoints, calibration, or configuration. A setting of
000 will bypass the access code. The factory setting is 000.
1. Press the Mode key until the Config indicator lights.
2. Press the right arrow until Acc Code XXX is displayed in the message
center.
3. Press up or down arrow to change the access code.
4. Press Enter to save the access code.
5. Press the Mode key to return to the Run mode or right/left to go to
next/previous parameter.
Turn All Audible
Alarms On/Off
Set an Access Code