Thermo Scientific Finnpipette User manual

1
Instructions for Use
Bedienungsanleitung
Guide d´utilisation et d´entretien
Instrucciones de uso
Fixed Volume
Finnpipette®
1 0 . 0
Finnpipette®
1 . 0 0
Thermo Electron Oy
P.O.Box 100, Fin-01621 Vantaa, Finland
Tel. [phone], fax -358-9-3291 0414
www.thermo.com/finnpipette

2
1 0 0 0
Finnpipette ®
Finnpipette 1 0 0 0
Finnpipette
1 0 0 0
R
A
C
B
1 2 3 4
A
C
B
1 2 3 4
4
3
A
C
B
1 2 3 4 5
A
C
B
1 2 3 4
5
6
8
9
10
11
1
2

3
CONTENTS
PRODUCT DESCRIPTION 4
PIPETTE OPERATION 4
PIPETTING TECHNIQUES 5
CALIBRATION 6
MAINTENANCE 8
TROUBLE SHOOTING 10
PACKAGE 10
SPARE PARTS 38
ENGLISH
INHALT
PRODUKTBESCHREIBUNG 11
HANDHABUNG DER FINNPIPETTE 11
PIPETTIER-TECHNIKEN 12
KALIBRIEREN 14
WARTUNG 16
STÖRUNGEN BEHEBEN 19
VERPACKUNG 19
ERSATZTEILE UND ZUBEHÖR 38
DEUTSCH
SOMMAIRE
DESCRIPTION 20
UTILISATION DE LA PIPETTE 20
CONSEILS DE PIPETAGE 21
CALIBRATION 22
MAINTENANCE 24
ERREURS ET CAUSES 27
CONDITIONNEMENT 27
PIÈCES DÉTACHÉES 38
FRANÇAIS
CONTENIDO
DESCRIPCION DEL PRODUCTO 28
FUNCIONES 28
TECNICAS DE PIPETEO 29
CALIBRACION 30
MAINTENIMIENTO 32
PROBLEMAS Y SOLUCIONES 34
PRESENTACION 34
PIEZAS DE RECAMBIO 38
ESPAÑOL
13
A
B
C
12
7

4
2
1
PRODUCT DESCRIPTION
The Finnpipette Fixed Volume is an autoclavable digital pipette. It operates on the air
displacement principle (i.e. an air interface) and uses detachable, disposable tips.
The delivery volume is displayed on a readout window on the handle.
The sixteen different models of Finnpipette Fixed Volume pipettes cover a volume
range from 1 µl to 10 ml.
Order No. Volume Range Finntip
4501000 1 µl 10
4501010 2 µl 10
4501020 5 µl 10
4501030 10 µl 10
4501040 20 µl 250, 200 Ext
4501050 25 µl 250, 200 Ext
4501060 50 µl 250, 300, 200 Ext
4501070 100 µl 250, 300, 200 Ext
4501080 200 µl 250, 300, 200 Ext
4501090 250 µl 1000
4501100 500 µl 1000
4501110 1000 µl 1000
4501120 2 ml 5 ml
4501130 3 ml 5 ml
4501140 5 ml 5 ml
4501150 10 ml 10 ml
DIGITAL DISPLAY
The delivery volume is clearly indicated in the large digital display on the handle.
RAW MATERIALS
The Finnpipette Fixed Volume is made of mechanically durable and chemically
resistant materials which allow repeated autoclaving of the complete pipette at 121°C.
DESCRIPTION OF TIPS
Finntips are recommended for use with the Finnpipette Fixed Volume.
They are made of natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
PIPETTEOPERATION
TIP EJECTION
To help eliminate the risk of contamination, each pipette is fitted with a tip ejector system.
The tip ejector system consists of a soft-touch tip ejector and specially designed
gearing mechanism. To release the tip, point the pipette at suitable waste receptacle
and press the tip ejector with your thumb.
ENGLISH

5
4
13
SAFETY LABEL
You can mark the pipette application your initials the calibration date, etc. on the safety label.
Remove the clear plastic window on the edge closest to the push button (use the
service tool that comes with the pipette, or a screwdriver). Mark the adhesive label
with a felt-tipped or other pen and snap the window back in place.
SHELF HANGER
You can attach the pipette shelf hanger on a counter, pipette stand or anywhere where
you want to hang your pipette.
Clean the area where you plan to attach the shelf hanger. Apply two stickers to the
underside of the shelf hanger. Press the shelf hanger firmly into place -- on a shelf,
countertop or pipette stand. To use, hang the grippy finger rest on the shelf hanger.
PIPETTING TECHNIQUES
Push and release the push button slowly at all times,
particularly when working with high viscosity liquids.
Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone.
Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with the
solution that you will be pipetting. Hold the pipette in an upright position while aspirating
liquid. The grippy should rest on your index finger. Make sure that the tips, pipette and
solution are at the same temperature.
FORWARD TECHNIQUE
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm
and slowly release the push button. Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push button to the first stop. After a
delay of about one second, continue to depress the push button all the way to the
second stop. This action will empty the tip.
4. Release the push button to the ready position.
If necessary, change the tip and continue pipetting.
REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very
small volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button. This action will fill the tip. Withdraw the tip from
the liquid touching it against the edge of the reservoir to remove excess liquid.
12
3
Figures 3-6:
A = Ready position
B = First stop
C = Second stop
ENGLISH

6
6
5
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
REPETITIVE TECHNIQUE
The repetitive technique offers a rapid and simple procedure for repeated delivery of
the same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button. This action will fill the tip. Withdraw the tip
from the liquid touching against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
PIPETTING WHOLE BLOOD
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the push button to the first stop,
making sure the tip is well below the surface.
2. Release the push button slowly to the ready position. This will fill the tip. Keep the
tip in the solution.
3. Depress the push button to the first stop and release slowly. Keep repeating this
procedure until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.
CALIBRATION
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified
with distilled or deionized water. The pipettes are constructed to permit re-adjustment
for liquids of different temperature and viscosity.
DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation value of the balance should
be chosen according to the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.00 1 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests
are done in a draft-free room at a constant (±0.5°C) temperature of water, pipette and
air between 15°C to 30°C. The relative humidity must be above 50%. Especially with
ENGLISH

7
8
ENGLISH
volumes under 50 µl the air humidity should be as high as possible to reduce the effect of
evaporation loss. Special accessories, such as the evaporation trap, are recommended.
PROCEDURE TO CHECK CALIBRATION
The pipette is checked with the maximum volume (nominal volume) and with the
minimum volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings
is done with both volumes. A pipette is always adjusted for delivery (Ex) of the
selected volume.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the accuracy (A) and precision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
TABLE 1: Maximum permissible errors according ISO8655
ADJUSTMENT
Adjustment is done with the service tool.
1. Place the service tool into the openings
of the calibration nut at the top of the handle.
2. Turn the service tool clockwise to
increase, or counterclockwise to
decrease the volume.
3. After adjustment check the calibration
according to the instructions above.
FORMULAS FOR CALCULATING
RESULTS
Conversion of mass to volume
V = (w + e) x Z
V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl conversion
Evaporation loss can be significant with low volumes. To determine mass loss,
dispense water to the weighing vessel, note the reading and start a stopwatch. See
how much the reading decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might
be 10 seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an
evaporation trap or lid on the vessel is used the correction of evaporation is usually
unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and
pressure. A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion
table on page 36.
Volume Accuracy Precision
µl µl % s.d.* µl cv%
1 ±0.04 ±4.0 0.04 4.0
2 ±0.05 ±2.5 0.04 2.0
5 ±0.07 ±1.4 0.07 1.4
10 ±0.09 ±0.9 0.08 0.8
20 ±0.12 ±0.6 0.10 0.5
25 ±0.15 ±0.6 0.13 0.5
50 ±0.3 ±0.6 0.2 0.4
100 ±0.4 ±0.4 0.3 0.3
200 ±0.8 ±0.4 0.6 0.3
250 ±1.0 ±0.4 0.8 0.3
500 ±1.5 ±0.3 1.5 0.3
1000 ±3.0 ±0.3 0.3 0.3
2000 ±6.0 ±0.3 4.0 0.2
3000 ±9.0 ±0.3 6.0 0.2
5000 ±15.0 ±0.3 10.0 0.2
10 ml ±30.0 ±0.3 20.0 0.2

8
9
8
ENGLISH
Accuracy (systematic error)
Accuracy is the difference between the dispensed volume and the selected volume of
a pipette.
A = V - V0A = accuracy
V = mean volume
V0= nominal volume
Accuracy can be expressed as a relative value: A% = 100% x A / V0
Precision (random error)
Precision refers to the repeatability of the pipettings. It is expressed as standard
deviation (s) or coefficient of variation (cv)
s = standards deviation
v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV) CV = 100% x S / V
MAINTENANCE
When the Finnpipette Fixed Volume is not in use, make sure it is stored in an upright
position. We recommend a Finnpipette stand for this purpose.
SHORT-TERM CHECKING
The pipette should be checked at the beginning of each day for dust and dirt on the
outside surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except 70 %
ethanol should be used to clean the pipette.
LONG-TERM MAINTENANCE
If the pipette is used daily it should be checked every three months. The servicing
procedure starts with the disassembly of the pipette.
DISASSEMBLING 1-40 µl PIPETTES
1. Press the tip ejector.
2. Insert the maintenance pliers under the ejector bar to release the tip ejector.
3. Remove the tip cone by pressing with maintenance pliers.
4. Pull out the piston and the spring.
5. Keep the tip cone vertically and push out with piston the rest of the piston assembly.
Then keep the tip cone upside down and tap all parts from tip cone. Remember
keep all parts in order on table for reassembly.
6. Clean the piston, the piston spring and the O-rings with a dry napless cloth.
7. Check the tip cone for foreign particles.
8. Grease the cleaned parts with the lubricant that comes with the pipette.
9. Reassemble the pipette components.
All 1-40 µl: First, slide the spring 14, spring support 15 and tube 16 back on the piston.
Compress the spring with fingers by pressing piston and spring support 15 agains eachother.
11-40 µl: Slide bigger O-ring 17, smaller O-ring 18, spring support 19 (sharp edges
against spring) and small spring 20 on the piston.
1
)(
2
-
-
=
n
VV
Si
S
i=1
n

9
8
10
11
8
9
14
ENGLISH
3-10 µl: First slide O-ring tube 17 (larger hole first), bigger O-ring 18, smaller O-
ring 19 and O-ring support 20 on the piston. Then slide small spring 21, spring
support 22 (sharp edges against spring) and O-ring 22 on the O-ring support 20.
1-2 µl: First slide O-ring tube 17 (larger hole first) and sealing combination 18 on
the piston. Then slide small spring 19, spring support 20 (sharp edges against
spring) and O-ring 21 on the sealing combination 18.
All 1-40 µl: Carefully slide the entire assembly into the tip cone and release your fingers.
10. With the push button depressed all the way carefully attach the tip cone to the
handle so that the adapter opening is on the tip ejector side. Do not bend the thin
piston wire when assembling. Press in the snap joints.
11. Assemble the tip ejector and check the calibration according to the instructions.
DISASSEMBLING 41-1000 µl PIPETTES
1. Press the tip ejector.
2. Insert the pliers under the ejector bar to release the tip ejector.
3. Remove the tip cone using the maintenance pliers.
4. Pull out the piston.
5. Remove the O-ring, O-ring support and spring from the tip cone.
6. Clean the piston, the piston spring and the O-ring with a dry napless cloth.
7. Check the cylinder for foreign particles.
8. Grease the cleaned parts with the lubricant that comes with the pipette.
9. Slide parts over the piston pressing down the large spring. Attach the tip cone to the
handle so that the adapter opening is on the tip ejector side, and press in the snap joints.
10. Check the calibration according to the instructions.
DISASSEMBLING 2-10 ml PIPETTES
1. Press the tip ejector.
2. Insert the pliers under the ejector bar to release the tip ejector.
3. Remove the part 2 from part 1 of the tip ejector using the maintenance pliers to
release the snap joint.
4. Remove the cylinder by pressing part 1 of the tip ejector firmly towards the cylinder.
This action releases the snap joint so you can remove the cylinder.
5. Clean the O-ring and cylinder. Regrease the O-ring and the cylinder.
6. Assemble the parts in the opposite order of disassembly. All joints are snap fit and
can be pushed together by hand. Be careful not to bend the pipette during
assembly because this could damage the snap joints or the piston.
7. Check the calibration according to the instructions.
STERILIZATION
The entire pipette can be sterilized by autoclaving it at 121°C (252°F) (minimum 20
minutes). No special preparations are needed for autoclaving. You can use steam
sterilization bags if needed.
After autoclaving the pipette must be cooled to room temperature for at least two hours.
Before pipetting, make sure that the pipette is dry. We recommend that you check the
calibration of 1-1000 µl pipettes after every 25th sterilization cycle and of 2-10 ml
pipettes after every 10th sterilization cycle.

10
Solution
Attach firmly
Clean tip cones attach
new tips
Clean and grease O-ring
and cylinder.
Grease accordingly
Change the O-ring
Follow instructions carefully
Attach firmly
Recalibrate according
to instructions
Recalibrate with
the liquids in question.
Defect
Leakage
Inaccurate dispensing
Inaccurate dispensing
with certain liquids
PACKAGE
The Finnpipette Fixed Volume is shipped in a specially designed package containing
the following items:
1. The Finnpipette 4. Finntip sample 7. Calibration certificate
2. Service tool 5. Tube of grease 8. Shelf hanger
3. Maintenance pliers 6. Instruction manual 9. Two stickers
TROUBLE SHOOTING
The table below lists possible problems and their solutions.
Possible reason
Tip incorrectly attached
Foreign particles
between tip and tip cone
Foreign particles between the
piston, the O-ring and the cylinder
Insufficient amount of grease on
cylinder and O-ring
O-ring damaged
Incorrect operation
Tip incorrectly attached
Calibration altered: caused
by misuse, for example
Unsuitable calibration.
High viscosity liquids may
require recalibration.
CAUTION!
The Finnpipette is designed to allow easy in-lab service. If you would
prefer to have us or your local representative service your pipette,
please make sure that the pipette has been decontaminated before you
send it to us.
Please note that the postal authorities in your country may prohibit or
restrict the shipment of contaminated material by mail.
ENGLISH