Thermo Scientific VF-F20-A User manual

*4827.6001-EN-1.0*
Vanquish
Integral Fraction
Collector FT
VF-F20-A
Operating Manual
4827.0601-EN Revision 1.0 •August 2022
thermoscientific

Copyright © 2022 Thermo Fisher Scientific Inc. All rights reserved.
Original Operation Manual
The hardware descriptions in this manual revision refer to the device VF-F20-A
Trademarks
Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated.
Microsoft and Windows are trademarks of Microsoft Corporation.
MP35N is a registered trademark of SPS Technologies.
PharMed is a registered trademark of Saint-Gobain Performance Plastics.
Torx is a trademark of Acument Intellectual Properties, LLC.
All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.
Disclaimer
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. The document is copyright protected; any reproduction of the
whole or any part of this document is strictly prohibited, except with the written authorization
of Thermo Fisher Scientific Inc.
This manual is provided "as is." The contents of this manual are subject to being changed,
without notice, in future revisions.
Thermo Fisher Scientific Inc. makes no representations that this document is complete,
accurate, or error-free. Thermo Fisher Scientific Inc. assumes no responsibility and will not be
liable for any errors, omissions, damage, or loss that might result from any use of this document,
even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale.
The Terms and Conditions of Sale shall govern all conflicting information between the two
documents.
Printed manual version only
Printed in Germany on 100% chlorine-free bleached, high-white paper that is produced in an
environmentally friendly process, leading to a paper profile of zero CO2 emissions.
Manufacturer's address
Dionex Softron GmbH, Part of Thermo Fisher Scientific, Dornierstrasse 4, D-82110 Germering.

Contacting Us
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 3
Contacting Us
There are several ways to contact us:
Ordering Information
For ordering information or sales support for HPLC products, contact
your local Thermo Fisher Scientific sales organization. For contact
information, go to Contact Us on http://www.thermoscientific.com.
Technical Assistance
For technical support for HPLC products, contact your local Thermo
Fisher Scientific support organization. For contact information, go to
Contact Us on http://www.thermoscientific.com.

Contacting Us
Page 4 Integral Fraction Collector FT (VF-F20-A) Operating Manual

Contacting Us
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 5
Contents
Contacting Us...................................................................................... 3
1 Using this Manual ...........................................................................10
1.1 About This Manual................................................................................................11
1.2 Conventions..........................................................................................................12
1.2.1 Safety Messages.......................................................................................12
1.2.2 Special Notices and Informational Notes.................................................12
1.2.3 Typographical Conventions......................................................................13
1.3 Reference Documentation....................................................................................15
2 Safety..............................................................................................18
2.1 Safety Symbols and Signal Words.........................................................................19
2.1.1 Safety Symbols and Signal Words in this Manual....................................19
2.1.2 Observing this Manual.............................................................................19
2.1.3 Safety Symbols on the Device..................................................................20
2.1.4 Applicable Symbols on the Device...........................................................20
2.1.5 Rating Plate..............................................................................................20
2.2 Intended Use.........................................................................................................21
2.3 Safety Precautions ................................................................................................22
2.3.1 General Safety Information .....................................................................22
2.3.2 Qualification of the Personnel .................................................................23
2.3.3 Personal Protective Equipment ...............................................................23
2.3.4 Electrical Safety Precautions....................................................................24
2.3.5 General Residual Hazards ........................................................................25
2.3.6 In Case of Emergency...............................................................................27
2.4 Solvent and Additive Information.........................................................................28
2.4.1 General Compatibility ..............................................................................28
2.4.2 Allowed pH Ranges ..................................................................................28
2.4.3 Allowed Concentrations...........................................................................29
2.4.4 Further Information .................................................................................29
2.5 Compliance Information.......................................................................................30
3 Device Overview .............................................................................32

Contacting Us
Page 6 Integral Fraction Collector FT (VF-F20-A) Operating Manual
3.1 Integral Fraction Collector Features .................................................................... 33
3.2 Operating Principle .............................................................................................. 34
3.3 Interior Components............................................................................................ 41
3.4 Fraction Collector Compartment ......................................................................... 43
3.4.1 Thermostatting........................................................................................ 43
3.4.2 Carousel................................................................................................... 44
3.4.3 Rack Type Identification.......................................................................... 44
3.4.4 Condensation Drainage........................................................................... 45
3.5 Leak Detection ..................................................................................................... 46
3.6 Operation............................................................................................................. 47
4 Unpacking and Transport................................................................49
4.1 Unpacking the Device .......................................................................................... 50
4.2 Scope of Delivery ................................................................................................. 54
5 Installation......................................................................................55
5.1 Safety Guidelines for Installation......................................................................... 56
5.2 Installing the Device............................................................................................. 57
5.3 Site Requirements................................................................................................ 59
5.3.1 Power Considerations ............................................................................. 59
5.3.2 Power Cord.............................................................................................. 60
5.3.3 Condensation .......................................................................................... 61
5.4 Accessing the Interior Components..................................................................... 62
5.5 Setting Up the Hardware ..................................................................................... 63
5.5.1 System Arrangement............................................................................... 63
5.5.2 Connecting the Device ............................................................................ 65
5.6 Setting Up the Flow Connections......................................................................... 68
5.6.1 General Information and Guidelines....................................................... 68
5.6.2 Guiding Capillaries and Tubing Through the System .............................. 69
5.6.3 Connecting Fittings, Capillaries, and Tubing ........................................... 71
5.6.4 Guiding Liquids to Waste ........................................................................ 78
5.7 Needle Wash System ........................................................................................... 80
5.7.1 Choosing the Needle Wash Liquid .......................................................... 81
5.7.2 Connecting the Needle Wash Reservoir ................................................. 81
5.8 Fraction Collection Valve ..................................................................................... 85
5.8.1 Port assignments of the Fraction Collection Valve ................................. 85

Contacting Us
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 7
5.9 Flush System .........................................................................................................86
5.9.1 Choosing the Flush Solvent......................................................................86
5.9.2 Connecting Flush Solvent Reservoir.........................................................86
5.9.3 Connecting the Delay Capillary................................................................90
5.9.4 Connecting the Flush Buffer Loop............................................................91
5.10 Turning On the Device ..........................................................................................93
5.11 Setting Up the Device in the Software..................................................................94
6 Operation........................................................................................96
6.1 Introduction to this Chapter.................................................................................97
6.2 Safety Guidelines for Operation ...........................................................................98
6.3 Control Elements ................................................................................................100
6.3.1 Keypad....................................................................................................100
6.3.2 Status Indicators ....................................................................................102
6.4 Power On/Off Control.........................................................................................104
6.5 Preparing the Device for Operation....................................................................105
6.5.1 Thermostatting the Fraction Collector Compartment...........................107
6.5.2 Loading the Carousel..............................................................................107
6.5.3 Rack Type Settings .................................................................................111
6.5.4 Needle Height Setting............................................................................111
6.6 Important Operating Parameters .......................................................................113
6.7 Delay Volume Determination (DVD)...................................................................120
6.7.1 Delay Volume and Time.........................................................................120
6.7.2 When to Perform DVD Function............................................................121
6.7.3 How to Perform DVD Function ..............................................................122
6.7.4 Delay Volume Capillary Drop Down Menu ............................................124
6.7.5 Vanquish Modules enabling Delay Volume Determination...................125
6.7.6 Vanquish Detector Flow Cells enabling Delay Volume Determination..126
6.7.7 Delay Volume Calculator........................................................................127
6.8 Vial Pusher Positions...........................................................................................132
6.8.1 Adjust to AboveVial Pusher Position......................................................133
6.8.2 Adjust to InVial Pusher Position.............................................................135
6.9 Optimizing the Performance of the Device ........................................................137
6.10 Shutting Down the Device ..................................................................................138
6.10.1 Short-Term Shutdown (Interruption of operation) ...............................138
6.10.2 Long-Term Shutdown.............................................................................139

Contacting Us
Page 8 Integral Fraction Collector FT (VF-F20-A) Operating Manual
7 Maintenance and Service..............................................................142
7.1 Introduction to Maintenance and Service......................................................... 143
7.2 Safety Guidelines for Maintenance and Service................................................ 144
7.3 General Rules for Maintenance and Service...................................................... 147
7.4 Routine and Preventive Maintenance ............................................................... 148
7.4.1 Maintenance Plan ................................................................................. 148
7.4.2 Cleaning or Decontaminating the Device.............................................. 149
7.4.3 Predictive Performance......................................................................... 151
7.5 Before Maintenance .......................................................................................... 153
7.5.1 Preparing the Device for Maintenance................................................. 153
7.5.2 Securing the Needle Drive..................................................................... 154
7.6 Removing the Front Plate .................................................................................. 155
7.7 Needle Wash Lines............................................................................................. 159
7.7.1 Needle Wash Liquid Guidelines ............................................................ 159
7.7.2 Replacing Needle Wash Lines ............................................................... 160
7.7.3 Performing A Needle Wash Cycle ......................................................... 162
7.8 Flush System ...................................................................................................... 164
7.8.1 Flush Solvent Guidelines ....................................................................... 164
7.8.2 Replacing Flush Purge Lines .................................................................. 165
7.8.3 Performing A Flush Purge Cycle............................................................ 171
7.9 Replacing the Needle Capillary .......................................................................... 172
7.10 Replacing the Needle Unit ................................................................................. 177
7.11 Replacing the Drain Pump Head with Tubing .................................................... 181
7.12 Replacing the Fraction Collection Valve Stator and Rotor................................. 183
7.13 After Maintenance............................................................................................. 186
7.14 Replacing the Main Power Fuses ....................................................................... 187
7.15 Updating the Device Firmware .......................................................................... 189
7.16 Replacing the Doors........................................................................................... 191
7.17 Transporting or Shipping the Device.................................................................. 193
7.18 Slide-In Module.................................................................................................. 197
7.18.1 Removing the Slide-In Module.............................................................. 197
7.18.2 Installing the Slide-In Module ............................................................... 199
8 Troubleshooting............................................................................202
8.1 General Information about Troubleshooting .................................................... 203

Contacting Us
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 9
8.2 Messages ............................................................................................................205
8.3 Operating Issues .................................................................................................212
8.3.1 Resolving Front Leakage ........................................................................212
8.3.2 Resolving Clogging in the Device ...........................................................213
8.3.3 Temperature Setpoint Cannot Be Achieved ..........................................214
9 Specifications................................................................................216
9.1 Performance Specifications ................................................................................217
9.2 Physical Specifications ........................................................................................220
10 Accessories, Consumables and Replacement Parts......................222
10.1 General Information ...........................................................................................223
10.2 Ship Kit ................................................................................................................224
10.3 Optional Accessories...........................................................................................226
10.4 Consumables and Replacement Parts ................................................................228
11 Appendix.....................................................................................232
11.1 Compliance Information.....................................................................................233
11.1.1 Declarations of Conformity....................................................................233
11.1.2 WEEE Compliance ..................................................................................234
11.1.3 FCC Compliance .....................................................................................235
12 Index...........................................................................................236

1•Using this Manual
Page 10 Integral Fraction Collector FT (VF-F20-A) Operating Manual
1Using this Manual
This chapter provides information about this manual, the conventions used
throughout the manual, and the reference documentation that is available
in addition to this manual.

1•Using this Manual
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 11
1.1 About This Manual
This manual describes the functional features and operating principle of
your Vanquish™device and provides instructions for installation, set up,
start up, shut down, operation, maintenance and troubleshooting.
The layout of this manual is designed to provide quick reference to the
sections of interest to the user. To obtain a full understanding of your
device, read this manual thoroughly.
This manual also contains safety messages, precautionary statements,
and special notices that can prevent personal injury, damage to the
device, or loss of data when followed properly.
Note the following:
•The device configuration may vary; therefore, not all descriptions
necessarily apply to your particular device.
•If some detail applies to only one model or variant, the model or
variant is identified by name.
•The descriptions in this manual refer to a Vanquish system
configuration with optical detector. Not all descriptions necessarily
apply to your particular system.
•Illustrations in this manual are provided for basic understanding.
They can vary from the actual model of the device or component.
However, this does not influence the descriptions. No claims can be
derived from the illustrations in this manual.
The descriptions in this manual assume that the device is installed in the
Vanquish system stack. If this is not the case, additional hardware is
required and must be ordered separately. The information in this
manual applies correspondingly.

1•Using this Manual
Page 12 Integral Fraction Collector FT (VF-F20-A) Operating Manual
1.2 Conventions
This section describes the conventions that are used throughout this
manual.
1.2.1 Safety Messages
The safety messages and precautionary statements in this manual
appear as follows:
•Safety messages or precautionary statements that apply to the
entire manual and all procedures in this manual are grouped in the
Safety chapter.
•Safety messages or precautionary statements that apply to an entire
section or to multiple procedures in a section appear at the
beginning of the section to which they apply.
•Safety messages that apply to only a particular section or procedure
appear in the section or procedure to which they apply. They appear
different from the main flow of text.
Safety messages are often preceded by an alert symbol and/or alert
word. The alert word appears in uppercase letters and in bold type.
Make sure that you understand and follow all safety messages
presented in this manual.
1.2.2 Special Notices and Informational Notes
Special notices and informational notes in this manual appear different
from the main flow of text. They appear in boxes and a note label
identifies them. The label text appears in uppercase letters and in bold
type.
NOTICE
Highlights information necessary to prevent damage to the device or
invalid test results.

1•Using this Manual
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 13
TIP
Highlights information of general interest or helpful information that can
make a task easier or optimize the performance of the device.
1.2.3 Typographical Conventions
These typographical conventions apply to the descriptions in this
manual:
Data Input and Output
The following appears in bold type:
Input that you enter by the keyboard or that you select with the
mouse
Buttons that you click on the screen
Commands that you enter by the keyboard
Names of, for example, dialog boxes, properties, and parameters
For brevity, long expressions and paths appear in the condensed form,
for example: Click Start > All Programs > Thermo Chromeleon 7 >
Services Manager > Start Instrument Controller.
References and Messages
References to additional documentation appear italicized.
•Messages that appear on the screen are identified by quotation
marks.
Viewpoint
If not otherwise stated, the expressions left and right in this manual
always refer to the viewpoint of a person that is facing the device from
the front.
Particularly Important Words
Particularly important words in the main flow of text appear italicized.

1•Using this Manual
Page 14 Integral Fraction Collector FT (VF-F20-A) Operating Manual
Electronic Manual Version (PDF)
The electronic version (PDF) of the manual contains numerous links that
you can click to go to other locations within the manual. These include:
•Table of contents entries
•Index entries
•Cross-references (in blue text), for example, to sections and figures

1•Using this Manual
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 15
1.3 Reference Documentation
In addition to this operating manual, other documentation is available
for reference.
Hardware Documentation
Additional hardware documentation includes the following:
•Operating manuals for the other modules of the Vanquish system
•Vanquish System Operating Manual
•Instrument Installation Qualification Operating Instructions
TIP Thermo Fisher Scientific provides up-to-date operating manuals as
PDF (Portable Document Format) files that you can access from our
customer manuals web site. To open and read the PDF files, Adobe™
Reader™or Adobe™Acrobat™is required. Go to the following web site:
www.thermofisher.com/HPLCmanuals.
Software Documentation
Additional software documentation includes the following:
•Chromeleon™ Help and documents
The Chromeleon Help provides extensive information and
comprehensive reference material for all aspects of the software.
In addition, the following documentation is available (availability
depends on the software version):
•Instrument Configuration Manager Help
For basic information about device installation and configuration,
refer to the Installation Guide; for specific information about a
certain device, refer to the Instrument Configuration Manager Help.
In Chromeleon 7, devices are called modules.
•Quick Start Guide
For information about the main elements of the user interface and
step-by-step guidance through the most important workflows, refer
to the Quick Start Guide.

1•Using this Manual
Page 16 Integral Fraction Collector FT (VF-F20-A) Operating Manual
TIP The Chromeleon Help and documents are included in the software
shipment.
Third-Party Documentation
Refer also to the user documentation provided by the manufacturers of
third-party components and materials, for example, Safety Data Sheets
(SDSs).

1•Using this Manual
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 17

2•Safety
Page 18 Integral Fraction Collector FT (VF-F20-A) Operating Manual
2Safety
This chapter provides general and specific safety information and informs
about the intended use of the device.

2•Safety
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 19
2.1 Safety Symbols and Signal Words
2.1.1 Safety Symbols and Signal Words in this Manual
This manual contains safety messages to prevent injury of the persons
using the device.
The safety symbols and signal words in this manual include the
following:
Always be aware of the safety information. Do not proceed until you
have fully understood the information and consider the consequences of
what you are doing.
CAUTION
Indicates a hazardous situation that, if not avoided, could result in minor
or moderate injury.
WARNING
Indicates a hazardous situation that, if not avoided, could result in
serious injury.
2.1.2 Observing this Manual
Observe the following:
•Before installing or operating the device, read this manual carefully
to be familiar with the device and this manual. The manual contains
important information with regard to user safety as well as use and
care of the device.
•Always keep the manual near the device for quick reference.
•Save this manual and pass it on to any subsequent user.
Read, understand, and comply with all safety messages and
precautionary statements presented in this manual.

2•Safety
Page 20 Integral Fraction Collector FT (VF-F20-A) Operating Manual
2.1.3 Safety Symbols on the Device
The table lists the safety symbols that appear on the device or on labels
affixed to the device. Follow the safety notices in this manual to prevent
the risk of operator injury or damage to the device.
Symbol
Description
Indicates a potential hazard. Refer to this manual to avoid the risk of
personal injury and/or to prevent damage to the device.
I

Power supply is on
Power supply is off
Indicates alternating current.
Indicates a potential pinch point hazard. Keep your hands clear to
avoid harming your hands.
2.1.4 Applicable Symbols on the Device
Symbol
Description
Need to remove the shipment lock before powering on the
device.
Consult instructions for use.
2.1.5 Rating Plate
The rating plate is present on the device near the electrical connections.
The rating plate indicates the serial number, part number, module
name, revision number, line and fuse rating, and the manufacturer's
address.
TIP An additional type label on the leak tray of the device indicates the
serial number, part number, module name, and revision number. To
facilitate device identification, have the information from this label
available when communicating with Thermo Fisher Scientific.
˜