Thermo Scientific VF-F20-A User manual

*4827.6001-EN-1.0*
Vanquish
Integral Fraction
Collector FT
VF-F20-A
Operating Manual
4827.0601-EN Revision 1.0 •August 2022
thermoscientific

Copyright © 2022 Thermo Fisher Scientific Inc. All rights reserved.
Original Operation Manual
The hardware descriptions in this manual revision refer to the device VF-F20-A
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Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 3
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Page 4 Integral Fraction Collector FT (VF-F20-A) Operating Manual

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Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 5
Contents
Contacting Us...................................................................................... 3
1 Using this Manual ...........................................................................10
1.1 About This Manual................................................................................................11
1.2 Conventions..........................................................................................................12
1.2.1 Safety Messages.......................................................................................12
1.2.2 Special Notices and Informational Notes.................................................12
1.2.3 Typographical Conventions......................................................................13
1.3 Reference Documentation....................................................................................15
2 Safety..............................................................................................18
2.1 Safety Symbols and Signal Words.........................................................................19
2.1.1 Safety Symbols and Signal Words in this Manual....................................19
2.1.2 Observing this Manual.............................................................................19
2.1.3 Safety Symbols on the Device..................................................................20
2.1.4 Applicable Symbols on the Device...........................................................20
2.1.5 Rating Plate..............................................................................................20
2.2 Intended Use.........................................................................................................21
2.3 Safety Precautions ................................................................................................22
2.3.1 General Safety Information .....................................................................22
2.3.2 Qualification of the Personnel .................................................................23
2.3.3 Personal Protective Equipment ...............................................................23
2.3.4 Electrical Safety Precautions....................................................................24
2.3.5 General Residual Hazards ........................................................................25
2.3.6 In Case of Emergency...............................................................................27
2.4 Solvent and Additive Information.........................................................................28
2.4.1 General Compatibility ..............................................................................28
2.4.2 Allowed pH Ranges ..................................................................................28
2.4.3 Allowed Concentrations...........................................................................29
2.4.4 Further Information .................................................................................29
2.5 Compliance Information.......................................................................................30
3 Device Overview .............................................................................32

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3.1 Integral Fraction Collector Features .................................................................... 33
3.2 Operating Principle .............................................................................................. 34
3.3 Interior Components............................................................................................ 41
3.4 Fraction Collector Compartment ......................................................................... 43
3.4.1 Thermostatting........................................................................................ 43
3.4.2 Carousel................................................................................................... 44
3.4.3 Rack Type Identification.......................................................................... 44
3.4.4 Condensation Drainage........................................................................... 45
3.5 Leak Detection ..................................................................................................... 46
3.6 Operation............................................................................................................. 47
4 Unpacking and Transport................................................................49
4.1 Unpacking the Device .......................................................................................... 50
4.2 Scope of Delivery ................................................................................................. 54
5 Installation......................................................................................55
5.1 Safety Guidelines for Installation......................................................................... 56
5.2 Installing the Device............................................................................................. 57
5.3 Site Requirements................................................................................................ 59
5.3.1 Power Considerations ............................................................................. 59
5.3.2 Power Cord.............................................................................................. 60
5.3.3 Condensation .......................................................................................... 61
5.4 Accessing the Interior Components..................................................................... 62
5.5 Setting Up the Hardware ..................................................................................... 63
5.5.1 System Arrangement............................................................................... 63
5.5.2 Connecting the Device ............................................................................ 65
5.6 Setting Up the Flow Connections......................................................................... 68
5.6.1 General Information and Guidelines....................................................... 68
5.6.2 Guiding Capillaries and Tubing Through the System .............................. 69
5.6.3 Connecting Fittings, Capillaries, and Tubing ........................................... 71
5.6.4 Guiding Liquids to Waste ........................................................................ 78
5.7 Needle Wash System ........................................................................................... 80
5.7.1 Choosing the Needle Wash Liquid .......................................................... 81
5.7.2 Connecting the Needle Wash Reservoir ................................................. 81
5.8 Fraction Collection Valve ..................................................................................... 85
5.8.1 Port assignments of the Fraction Collection Valve ................................. 85

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5.9 Flush System .........................................................................................................86
5.9.1 Choosing the Flush Solvent......................................................................86
5.9.2 Connecting Flush Solvent Reservoir.........................................................86
5.9.3 Connecting the Delay Capillary................................................................90
5.9.4 Connecting the Flush Buffer Loop............................................................91
5.10 Turning On the Device ..........................................................................................93
5.11 Setting Up the Device in the Software..................................................................94
6 Operation........................................................................................96
6.1 Introduction to this Chapter.................................................................................97
6.2 Safety Guidelines for Operation ...........................................................................98
6.3 Control Elements ................................................................................................100
6.3.1 Keypad....................................................................................................100
6.3.2 Status Indicators ....................................................................................102
6.4 Power On/Off Control.........................................................................................104
6.5 Preparing the Device for Operation....................................................................105
6.5.1 Thermostatting the Fraction Collector Compartment...........................107
6.5.2 Loading the Carousel..............................................................................107
6.5.3 Rack Type Settings .................................................................................111
6.5.4 Needle Height Setting............................................................................111
6.6 Important Operating Parameters .......................................................................113
6.7 Delay Volume Determination (DVD)...................................................................120
6.7.1 Delay Volume and Time.........................................................................120
6.7.2 When to Perform DVD Function............................................................121
6.7.3 How to Perform DVD Function ..............................................................122
6.7.4 Delay Volume Capillary Drop Down Menu ............................................124
6.7.5 Vanquish Modules enabling Delay Volume Determination...................125
6.7.6 Vanquish Detector Flow Cells enabling Delay Volume Determination..126
6.7.7 Delay Volume Calculator........................................................................127
6.8 Vial Pusher Positions...........................................................................................132
6.8.1 Adjust to AboveVial Pusher Position......................................................133
6.8.2 Adjust to InVial Pusher Position.............................................................135
6.9 Optimizing the Performance of the Device ........................................................137
6.10 Shutting Down the Device ..................................................................................138
6.10.1 Short-Term Shutdown (Interruption of operation) ...............................138
6.10.2 Long-Term Shutdown.............................................................................139

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7 Maintenance and Service..............................................................142
7.1 Introduction to Maintenance and Service......................................................... 143
7.2 Safety Guidelines for Maintenance and Service................................................ 144
7.3 General Rules for Maintenance and Service...................................................... 147
7.4 Routine and Preventive Maintenance ............................................................... 148
7.4.1 Maintenance Plan ................................................................................. 148
7.4.2 Cleaning or Decontaminating the Device.............................................. 149
7.4.3 Predictive Performance......................................................................... 151
7.5 Before Maintenance .......................................................................................... 153
7.5.1 Preparing the Device for Maintenance................................................. 153
7.5.2 Securing the Needle Drive..................................................................... 154
7.6 Removing the Front Plate .................................................................................. 155
7.7 Needle Wash Lines............................................................................................. 159
7.7.1 Needle Wash Liquid Guidelines ............................................................ 159
7.7.2 Replacing Needle Wash Lines ............................................................... 160
7.7.3 Performing A Needle Wash Cycle ......................................................... 162
7.8 Flush System ...................................................................................................... 164
7.8.1 Flush Solvent Guidelines ....................................................................... 164
7.8.2 Replacing Flush Purge Lines .................................................................. 165
7.8.3 Performing A Flush Purge Cycle............................................................ 171
7.9 Replacing the Needle Capillary .......................................................................... 172
7.10 Replacing the Needle Unit ................................................................................. 177
7.11 Replacing the Drain Pump Head with Tubing .................................................... 181
7.12 Replacing the Fraction Collection Valve Stator and Rotor................................. 183
7.13 After Maintenance............................................................................................. 186
7.14 Replacing the Main Power Fuses ....................................................................... 187
7.15 Updating the Device Firmware .......................................................................... 189
7.16 Replacing the Doors........................................................................................... 191
7.17 Transporting or Shipping the Device.................................................................. 193
7.18 Slide-In Module.................................................................................................. 197
7.18.1 Removing the Slide-In Module.............................................................. 197
7.18.2 Installing the Slide-In Module ............................................................... 199
8 Troubleshooting............................................................................202
8.1 General Information about Troubleshooting .................................................... 203

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8.2 Messages ............................................................................................................205
8.3 Operating Issues .................................................................................................212
8.3.1 Resolving Front Leakage ........................................................................212
8.3.2 Resolving Clogging in the Device ...........................................................213
8.3.3 Temperature Setpoint Cannot Be Achieved ..........................................214
9 Specifications................................................................................216
9.1 Performance Specifications ................................................................................217
9.2 Physical Specifications ........................................................................................220
10 Accessories, Consumables and Replacement Parts......................222
10.1 General Information ...........................................................................................223
10.2 Ship Kit ................................................................................................................224
10.3 Optional Accessories...........................................................................................226
10.4 Consumables and Replacement Parts ................................................................228
11 Appendix.....................................................................................232
11.1 Compliance Information.....................................................................................233
11.1.1 Declarations of Conformity....................................................................233
11.1.2 WEEE Compliance ..................................................................................234
11.1.3 FCC Compliance .....................................................................................235
12 Index...........................................................................................236

1•Using this Manual
Page 10 Integral Fraction Collector FT (VF-F20-A) Operating Manual
1Using this Manual
This chapter provides information about this manual, the conventions used
throughout the manual, and the reference documentation that is available
in addition to this manual.