Thermo Scientific Dionex ICS-5000+ User manual

Dionex ICS-5000+Ion Chromatography
System Installation Instructions
Document No. 065447
Revision 02
December 2014

© 2014 Thermo Fisher Scientific Inc. All rights reserved.
Chromeleon is a registered trademark of Thermo Fisher Scientific Inc. in the
United States. Microsoft and Windows are registered trademarks of Microsoft
Corporation in the United States and other countries.
The following are registered trademarks in the United States and possibly
other countries: KIMWIPES is a registered trademark of Kimberly-Clark
Corporation. Ultem is a registered trademark of General Electric Company.
PEEK is a trademark of Victrex PLC.
All other trademarks are the property of Thermo Fisher Scientific Inc. and its
subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a
product purchase to use in the product operation. This document is copyright
protected and any reproduction of the whole or any part of this document is
strictly prohibited, except with the written authorization of Thermo Fisher
Scientific Inc.
The contents of this document are subject to change without notice. All
technical information in this document is for reference purposes only. System
configurations and specifications in this document supersede all previous
information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this
document is complete, accurate, or error-free and assumes no
responsibility and will not be liable for any errors, omissions, damage, or
loss that might result from any use of this document, even if the
information in the document is followed properly.
Revision history: Revision 01 released November 2012; initial release of
instrument
Revision 02 released December 2014; support for low-temperature Dionex
ICS-5000+Detector/Chromatography Module
Software versions: Chromeleon 7.1 SR2 (or later) or Chromeleon 6.8 SR12
(or later) for a system including a standard DC; Chromeleon 7.2 SR2 (or later)
for a system including a low-temperature DC
For Research Use Only. Not for use in diagnostic procedures.

Doc. 065447-02 12/14 i
1 • Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.4 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.5 Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.6 Fitting and Tube Connection Guidelines . . . . . . . . . . . . . . . . . . . . . . . 9
1.6.1 Connecting High-Pressure Tube Fittings . . . . . . . . . . . . . . . 10
1.6.2 Tightening High-Pressure Fittings . . . . . . . . . . . . . . . . . . . . 11
1.6.3 Cutting Non-Capillary Tubing . . . . . . . . . . . . . . . . . . . . . . . 11
2 • Unpacking Instructions
2.1 Unpacking Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.2 Unpacking the Dionex AS-AP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.3 Unpacking the Dionex ICS-5000+Modules . . . . . . . . . . . . . . . . . . . 19
2.4 Installing the Safety Support Bracket . . . . . . . . . . . . . . . . . . . . . . . . 21
2.5 Removing the Pump Shipping Screws (Optional) . . . . . . . . . . . . . . . 23
2.6 Unpacking the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
2.7 Installing the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
2.8 Unpacking Consumable Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Contents

Dionex ICS-5000+Installation Instructions
ii Doc. 065447-02 12/14
3 • System Setup
3.1 System Setup Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
3.2 Connecting the Power Cords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
3.3 Plugging in the Dionex EGC and CR-TC (RFIC-EG System Only) .29
3.4 Setting Up the DC for Capillary IC Applications . . . . . . . . . . . . . . . .32
3.4.1 Installing the IC Cube Component Cartridges . . . . . . . . . . .33
3.4.2 Installing the EG Degas, CRD, or CES Cartridge . . . . . . . . .34
3.4.3 Installing the Detector in the DC . . . . . . . . . . . . . . . . . . . . . .34
3.5 Setting Up the DC for Analytical IC Applications . . . . . . . . . . . . . . .36
3.5.1 Installing the CD or ED . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
3.5.2 Installing the Suppressor in the DC . . . . . . . . . . . . . . . . . . . .37
3.6 Setting Up the TC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
3.6.1 Installing the Temperature Stabilizer in the TC (Optional) . .38
3.6.2 Installing the Columns in the TC . . . . . . . . . . . . . . . . . . . . . .40
3.6.3 Installing the TC Column ID (Identification) System . . . . . .41
3.7 Connecting the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
3.7.1 Connecting the Autosampler Sample Transfer Line . . . . . . .42
3.7.2 Connecting the Injection Valve Waste Line . . . . . . . . . . . . .43
3.8 Connecting Modules to the Computer . . . . . . . . . . . . . . . . . . . . . . . .45
3.8.1 USB Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
3.8.2 USB Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
3.8.3 USB Connection Examples . . . . . . . . . . . . . . . . . . . . . . . . . .47
3.9 Setting Up the Chromatography Software . . . . . . . . . . . . . . . . . . . . .49
3.9.1 Installing the Software and License . . . . . . . . . . . . . . . . . . .49

Contents
Doc. 065447-02 12/14 iii
3.9.2 Starting the Instrument Controller Service or Server . . . . . . 49
3.9.3 Installing the USB Device Drivers . . . . . . . . . . . . . . . . . . . . 50
3.9.4 Configuring the Modules in Chromeleon . . . . . . . . . . . . . . . 51
3.9.5 Selecting Configuration Properties . . . . . . . . . . . . . . . . . . . 52
3.9.6 Starting the Chromeleon Client . . . . . . . . . . . . . . . . . . . . . . 58
3.10 Connecting Drain Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
3.11 Degassing the Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
3.12 Setting Up the Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
3.13 Setting Up the Piston Seal Wash System . . . . . . . . . . . . . . . . . . . . . 67
3.13.1 Setting Up the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
3.13.2 Activating the Piston Seal Wash Function . . . . . . . . . . . . . . 68
3.14 Priming the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
3.15 Checking the Conductivity of the Water . . . . . . . . . . . . . . . . . . . . . . 70
4 • System Plumbing
4.1 Plumbing a Capillary IC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.1.1 Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.1.2 System Plumbing Checklists . . . . . . . . . . . . . . . . . . . . . . . . 73
4.1.3 Installing the Trap Column for KOH-Based Separations . . 74
4.1.4 Connecting the Pump Outlet . . . . . . . . . . . . . . . . . . . . . . . . 75
4.1.5 Filling and Flushing the CD Regenerant Path . . . . . . . . . . . 75
4.1.6 Checking the Background Conductivity . . . . . . . . . . . . . . . 77
4.1.7 Completing the Installation Qualification . . . . . . . . . . . . . . 77
4.1.8 Completing the Operational Qualification . . . . . . . . . . . . . . 78

Dionex ICS-5000+Installation Instructions
iv Doc. 065447-02 12/14
4.1.9 Filling and Flushing the ED Liquid Path . . . . . . . . . . . . . . . .78
4.1.10 Flushing and Conditioning the Electrolytic Devices . . . . . . .79
4.1.11 Flushing and Installing the Columns . . . . . . . . . . . . . . . . . . .84
4.1.12 Completing the Plumbing for Conductivity Detection . . . . .90
4.1.13 Completing the Plumbing for Electrochemical Detection . . .92
4.1.14 Routing the IC Cube Tubing . . . . . . . . . . . . . . . . . . . . . . . . .93
4.2 Plumbing an Analytical IC System . . . . . . . . . . . . . . . . . . . . . . . . . .95
4.2.1 Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
4.2.2 System Plumbing Checklists . . . . . . . . . . . . . . . . . . . . . . . . .96
4.2.3 Plumbing the Static Mixer or Trap Column . . . . . . . . . . . . .96
4.2.4 Completing the IQ Checklist . . . . . . . . . . . . . . . . . . . . . . . . .98
4.2.5 Performing the System Function Test . . . . . . . . . . . . . . . . . .99
4.2.6 Completing the Operational Qualification . . . . . . . . . . . . . .99
4.2.7 Plumbing the EG for Analytical IC Applications . . . . . . . . .99
4.2.8 Plumbing the DC for Analytical IC Applications . . . . . . . .110
4.2.9 Plumbing the TC for Analytical IC Applications . . . . . . . .114
4.3 Connecting Waste Lines to the Gas Separator Waste Tube . . . . . . .116
4.4 Equilibrating the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .118
4.4.1 Installing a Backpressure Coil . . . . . . . . . . . . . . . . . . . . . . .120
A • Installing Pump Options
A.1 Pressurizing Eluent Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
A.1.1 Installing the TC/VWD/PDA Regulator Bracket . . . . . . . .122
A.1.2 Connecting the Gas Source . . . . . . . . . . . . . . . . . . . . . . . . .122

Contents
Doc. 065447-02 12/14 v
A.1.3 Pressurizing the Eluent Reservoir . . . . . . . . . . . . . . . . . . . 124
A.2 Replumbing the Seal Wash System (DP only) . . . . . . . . . . . . . . . . 125
B • Installing DC Options
B.1 Installing the ED Amperometry Cell . . . . . . . . . . . . . . . . . . . . . . . . 127
B.1.1 Unpacking and Disassembling the Cell . . . . . . . . . . . . . . . 127
B.1.2 Installing a Disposable Working Electrode . . . . . . . . . . . . 130
B.1.3 Installing a Conventional Working Electrode . . . . . . . . . . 130
B.1.4 Installing the pH-Ag/AgCl Reference Electrode . . . . . . . . 133
B.1.5 Installing the PdH Reference Electrode in a Capillary IC
System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
B.2 Installing Low-Pressure Valves in the DC . . . . . . . . . . . . . . . . . . . 141
B.2.1 Installing a Low-Pressure Valve on the DC Rear Panel . . 141
B.2.2 Installing a Low-Pressure Valve Inside the DC . . . . . . . . . 142
B.3 Installing the Injection Valve Panel in the DC . . . . . . . . . . . . . . . . 143
B.4 Installing the Automation Manager (AM) in the DC . . . . . . . . . . . 146
B.4.1 Installing a High-Pressure Valve on the AM . . . . . . . . . . . 148
B.4.2 Installing a Low-Pressure Valve on the AM . . . . . . . . . . . 149
B.4.3 Installing the RCH-1 Reaction Coil Heater on the AM . . . 149
B.4.4 Installing the Temperature Stabilizer on the AM . . . . . . . . 150
B.4.5 Installing the AM in the DC Upper Compartment . . . . . . . 150
B.5 Installing an IC Cube in the DC . . . . . . . . . . . . . . . . . . . . . . . . . . . 152

Dionex ICS-5000+Installation Instructions
vi Doc. 065447-02 12/14
C • Networking Chromeleon 6.8 Computers
C.1 Setting Up a Shared Datasource . . . . . . . . . . . . . . . . . . . . . . . . . . . .158
C.1.1 Setting Up a Shared Datasource on a Workgroup . . . . . . . .158
C.1.2 Setting Up a Shared Datasource on a Domain . . . . . . . . . .159
C.2 Connecting a Client to a Remote Datasource . . . . . . . . . . . . . . . . . .162
C.2.1 Connecting a Server to a Remote Datasource . . . . . . . . . . .163
C.3 Connecting a Client and Server Through a Network . . . . . . . . . . . .163

Doc. 065447-02 12/14 1
1 • Introduction
1.1 Overview
The modular design of the Thermo Scientific Dionex™ICS-5000+Ion
Chromatography System lets you configure and customize components for a
wide range of applications. This manual provides instructions for initial
installation of the components that make up typical ion chromatography (IC)
systems and Reagent-Free™IC (RFIC™) systems with eluent generation
(RFIC-EG™). Configurations for both capillary IC and analytical IC
applications are described. Analytical IC applications can be configured with
either standard bore or microbore plumbing.
Installation instructions for the following Thermo Scientific modules and
components are included:
•Dionex ICS-5000+Dual Pump (DP)
•Dionex ICS-5000+Single Pump (SP)
•Dionex ICS-5000+Eluent Generator (EG)
•Dionex ICS-5000+Detector/Chromatography Module (DC)
•Dionex ICS-5000+Conductivity Detector (CD)
•Dionex ICS-5000+Electrochemical Detector (ED)
•Dionex ICS-5000+IC Cube™(IC Cube)
•Dionex ICS-5000+Automation Manager (AM)
•Dionex ICS-5000+Thermal Compartment (TC)
•Dionex ICS Series AS-AP Autosampler
•Dionex AS-DV Autosampler

Dionex ICS-5000+Installation Instructions
2Doc. 065447-02 12/14
1.2 Related Documentation
In addition to this manual, the following documents are provided on the
Thermo Scientific Reference Library DVD (P/N 053891).
•Dionex ICS-5000+Ion Chromatography System Operator’s Manual
(Document No. 065447)
•Dionex ICS Series Photodiode Array Detector Operator’s Manual
(Document No. 065147)
•Dionex ICS Series Variable Wavelength Detector Operator’s Manual
(Document No. 065141)
•Dionex AS-AP Autosampler Operator’s Manual (Document No. 065361)
•Dionex AS-DV Autosampler Operator’s Manual (Document No. 065259)
•Manuals for consumable products (columns, suppressors, CR-TC, EGC,
CRD)
•Chromeleon 7 Installation Guide (Document No. 7229.0003)
•Installing Chromeleon 6.8 with a Dionex Ion Chromatograph (Document
No. 031883)
1.3 Safety Messages and Notes
This manual contains warnings and precautionary statements that can prevent
personal injury and/or damage to the system when properly followed. Safety
messages appear in bold type and are accompanied by icons, as shown below.
Indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
Indicates a potentially hazardous situation which, if not avoided, may
result in death or serious injury.
Indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate injury.

1 • Introduction
Doc. 065447-02 12/14 3
Messages d'avertissement en français
Warnhinweise in Deutsch
Notes
Informational messages also appear throughout the manual. These are labeled
NOTE and are in bold type:
NOTE NOTES call attention to certain information. They alert you
to an unexpected result of an action, suggest how to optimize
instrument performance, etc.
Indicates that the function or process of the instrument may be
impaired. Operation does not constitute a hazard.
Signale une situation de danger immédiat qui, si elle n'est pas évitée,
entraînera des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures mineures à modérées. Également
utilisé pour signaler une situation ou une pratique qui pourrait
gravement endommager l'instrument mais qui n'entraînera pas de
blessures.
Bedeutet unmittelbare Gefahr. Mißachtung kann zum Tod oder
schwerwiegenden Verletzungen führen.
Bedeutet eine mögliche Gefährdung. Mißachtung kann zum Tod oder
schwerwiegenden Verletzungen führen.
Bedeutet eine mögliche Gefährdung. Mißachtung kann zu kleineren
oder mittelschweren Verletzungen führen. Wird auch verwendet, wenn
eine Situation zu schweren Schäden am Gerät führen kann, jedoch
keine Verletzungsgefahr besteht.

Dionex ICS-5000+Installation Instructions
4Doc. 065447-02 12/14
Sections preceded by the Capillary IC flag contain information that applies to
capillary IC systems only.
Sections preceded by the Analytical IC flag contain information that applies
to analytical IC systems only.
If a section is not flagged, the information in the section applies to both
capillary IC and analytical IC systems.
1.4 Facility Requirements
•Make sure the Dionex ICS-5000+system installation site meets the power
and environmental specifications for each module. Refer to Appendix A
of Dionex ICS-5000+Ion Chromatography System Operator’s Manual
(Document No. 065446) for specifications. The manual is provided on the
Thermo Scientific Reference Library DVD (P/N 053891).
•Make sure the installation site has enough power outlets for all system
components.
•Provide a sturdy, vibration-free lab bench of a height that ensures
convenient access to the interior of each Dionex ICS-5000+module.
•Allow at least 6 cm (2.4 in) of clearance behind and at least 2.5 cm (1 in)
to the sides of the system for power connections and ventilation.
•Provide a suitable waste container or drain system for system waste and
sample waste.
Capillary
IC

1 • Introduction
Doc. 065447-02 12/14 5
•For eluent generation, or when manually preparing eluent and regenerant,
use ASTM Type I (18 megohm-cm) filtered and deionized water that
meets the specifications listed in Table 1-1.
•(Optional) If eluent reservoirs will be pressurized, provide a clean helium
or nitrogen source regulated to approximately 0.17 to 0.34 MPa (25 to
50 psi).
•(Optional) For pressurized reagent delivery, provide a clean helium or
nitrogen source regulated to between 0.60 and 0.85 MPa (80 and 120 psi).
Contaminant Specification
Ions–Resistivity >18.0 (megohm-cm)
Organics–TOC <10 ppb
Iron/Transition Metals* <1 ppb
Pyrogens <0.03 (Eu/mL)
Particulates >0.2 µm <1 (units/mL)
Colloids–Silica <10 ppb
Bacteria <1 (cfu/mL)
* Iron/transition metal content not specified for ASTM
Type I water
Table 1-1. ASTM Filtered, Type I Deionized Water
Specifications for Ion Chromatography
Never pressurize eluent reservoirs above 0.07 MPa (10 psi).
Pressurizing reservoirs above this limit can cause the reservoir to
explode.
Ne mettez jamais les réservoirs d'éluants sous une pression supérieure
à 0,07 MPa (10 lb/po²).
Setzen Sie den Eluentbehälter auf keinen Fall einem Druck über
0,07 MPa aus.

Dionex ICS-5000+Installation Instructions
6Doc. 065447-02 12/14
1.5 Configurations
The table below describes some common Dionex ICS-5000+system
configurations.
System Configuration Typical Modules and Components Included
Single-Analysis Capillary
IC or RFIC-EG System with
Conductivity Detector
•SP
•DC with one IC Cube, including an injection
valve, and capillary versions of the following:
EG degasser, suppressor, and Thermo
Scientific Dionex Carbonate Removal
Device (CRD) (an option for anion analyses)
•Capillary CD
•Capillary column set
•Dionex AS-AP
•For RFIC-EG: EG with one capillary Thermo
Scientific Dionex EGC (Dionex EGC) and
one capillary Thermo Scientific Dionex
Continuously Regenerated Trap Column
(Dionex CR-TC)
Single-Analysis Capillary
IC or RFIC-EG System with
Electrochemical Detector
•SP
•DC with one IC Cube, including an injection
valve and a capillary EG degasser
•Capillary ED
•Capillary column set
•Dionex AS-AP
•For RFIC-EG: EG with one capillary Dionex
EGC and one capillary Dionex CR-TC
Single-Analysis Analytical
IC or RFIC-EG System with
Conductivity Detector
•SP
•DC with one injection valve, one analytical
column set, and one analytical suppressor
•Analytical CD
•Dionex AS-AP
•For RFIC-EG: EG with one analytical Dionex
EGC, one analytical Dionex CR-TC, and one
Thermo Scientific Dionex RFIC+Eluent
Degasser

1 • Introduction
Doc. 065447-02 12/14 7
Single-Analysis Analytical
IC or RFIC-EG System with
Electrochemical Detector
•SP
•DC with one injection valve and one
analytical column set
•Analytical ED
•Dionex AS-AP
•For RFIC-EG: EG with one analytical Dionex
EGC, one analytical Dionex CR-TC, and one
Dionex RFIC+Eluent Degasser
Single-Analysis Analytical
IC or RFIC-EG System with
Variable Wavelength
Detector, Photodiode Array
Detector, or Mass
Spectrometer
•SP
•TC with one injection valve and one
analytical column set
•One detector: Thermo Scientific Dionex ICS
Series Variable Wavelength Detector
(Dionex VWD), Thermo Scientific Dionex
ICS Series Photodiode Array Detector
(Dionex PDA), or Thermo Scientific MSQ™
Mass Spectrometer
•Dionex AS-AP
•For RFIC-EG: EG with one analytical Dionex
EGC, one analytical Dionex CR-TC, and one
Dionex RFIC+Eluent Degasser
Dual-Analysis Capillary IC
or RFIC-EG System •DP
•DC with two IC Cubes
•Two capillary detectors—two CDs, two EDs,
or one CD and one ED
•Dionex AS-AP
•For RFIC-EG: EG with two capillary Dionex
EGCs and two capillary Dionex CR-TCs
System Configuration Typical Modules and Components Included

Dionex ICS-5000+Installation Instructions
8Doc. 065447-02 12/14
For detailed plumbing diagrams for these configurations, refer to Chapter 3,
“Configurations,” in Dionex ICS-5000+Ion Chromatography System
Operator’s Manual (Document No. 065446). The manual is provided on the
Thermo Scientific Reference Library DVD (P/N 053891).
Dual-Analysis Analytical IC
or RFIC-EG System •DP
•DC with two injection valves, two analytical
column sets, and two analytical detectors—
two CDs, two EDs, or one CD and one ED;
-or-
•TC with two injection valves, two column
sets, and two detectors
•Dionex AS-AP
•For RFIC-EG: EG with two Dionex EGCs,
two Dionex CR-TCs, and two Dionex RFIC+
Eluent Degassers
Dual-Analysis Capillary
and Analytical RFIC-EG
Hybrid System
•DP with one capillary pump and one
analytical pump
•DC with one IC Cube in the upper
compartment, one column set and one
injection valve in the lower compartment,
and two detectors
•EG with one capillary Dionex EGC and one
analytical Dionex EGC
System Configuration Typical Modules and Components Included

1 • Introduction
Doc. 065447-02 12/14 9
Figure 1-1 illustrates a typical dual-analysis IC system.
1.6 Fitting and Tube Connection Guidelines
Follow these guidelines when connecting tube fittings in the Dionex ICS-
5000+system.
•Use high-pressure fittings, 10-32 fitting bolts (P/N 074449), and high-
pressure 10-32 double-cone ferrules (P/N 074373). These fittings are used
even in low-pressure systems.
•When connecting tubing to a port, make sure the fitting bolt and ferrule
are at least 2 mm (0.1 in) from the end of the tubing before you insert the
tubing into the port. Do not position the ferrule and fitting bolt flush with
the end of the tubing.
•If you are installing a capillary system, do not shorten or lengthen the
0.062 mm (0.0025 in) ID blue PEEK™(polyether ether ketone) tubing
(capillary tubing) used for Dionex IC Cube connections, and do not
Figure 1-1. Dionex ICS-5000+System for Dual-Analysis RFIC-EG
1
2
34
Dionex ICS-5000+Detector/
Chromatography Module
Dionex ICS-5000+Dual
Pump (DP)
Dionex ICS-5000+
Eluent Generator (EG)
Dionex ICS Series
AS-AP Autosampler
1
2
3
4
Capillary
IC

Dionex ICS-5000+Installation Instructions
10 Doc. 065447-02 12/14
substitute different tubing. This tubing is precision cut at the factory,
using a specialized cutter. Precision cut tubing is included in the Dionex
IC Cube Tubing Kit (P/N 072186).
1.6.1 Connecting High-Pressure Tube Fittings
1. Install the fitting bolt and ferrule onto the tubing. Position the ferrule at
least 2 mm (0.1 in) from the end of the tubing (see Figure 1-2).
2. Insert the tubing into the port until it stops.
3. While maintaining pressure on the tubing to keep it in place in the port,
tighten the fitting bolt fingertight and then tighten the fitting further by
following the instructions in Section 1.6.2.
Figure 1-2. Ferrule and Fitting Bolt Placement for Tubing Connections
Figure 1-3. Ferrule and Fitting Bolt Placement for Tubing Connections
Correct placement of ferrule
and fitting on tubing
Incorrect placement of ferrule
and fitting on tubing


1 • Introduction
Doc. 065447-02 12/14 11
1.6.2 Tightening High-Pressure Fittings
1. Use your fingers to tighten the fitting bolt as tight as you can.
2. After finger-tightening, use a wrench to tighten the fitting an additional
three-quarter turn (270 degrees).
3. If leaks occur, replace the fitting bolt (P/N 074449), ferrule (P/N 074373),
and tubing.
1.6.3 Cutting Non-Capillary Tubing
If a connection does not require precision cut capillary tubing, you can use a
tubing cutter to cut tubing to the required length. Make sure the cut is at a
right angle to the length of the tubing and there are no nicks or burrs on the
end. A tubing cutter (P/N 049584) is included in the DC Ship Kit
(P/N 072011, standard DC; P/N 22171-62000, low-temperature DC). Refer to
the instructions provided with the cutter.

Dionex ICS-5000+Installation Instructions
12 Doc. 065447-02 12/14