Thermo Scientific Evolution 300 User manual

Evolution™ 300 and Evolution 600
User Guide

The information in this publication is provided for reference only. All information
contained in this publication is believed to be correct and complete. Thermo Fisher
Scientific shall not be liable for errors contained herein nor for incidental or consequential
damages in connection with the furnishing, performance or use of this material. All
product specifications, as well as the information contained in this publication, are subject
to change without notice.
This publication may contain or reference information and products protected by
copyrights or patents and does not convey any license under our patent rights, nor the
rights of others. We do not assume any liability arising out of any infringements of patents
or other rights of third parties.
We make no warranty of any kind with regard to this material, including but not limited
to the implied warranties of merchantability and fitness for a particular purpose.
Customers are ultimately responsible for validation of their systems.
© 2005-2007 Thermo Fisher Scientific Inc. All rights reserved. No part of this publication
may be stored in a retrieval system, transmitted, or reproduced in any way, including but
not limited to photocopy, photograph, magnetic or other record, without our prior written
permission.
For technical assistance, please contact:
Technical Support
Thermo Fisher Scientific
5225 Verona Road
Madison, WI 53711-4495
U.S.A.
Telephone: [phone] (U.S.A.) or [phone] (worldwide)
Fax: [phone] (worldwide)
E-mail: [email protected]
World Wide Web: http://www.thermo.com/spectroscopy
Microsoft, Windows and Windows NT are either trademarks or registered trademarks of
Microsoft Corporation in the United States and/or other countries. Spectralon is a
registered trademark of Labsphere, Inc. All other trademarks are the property of Thermo
Fisher Scientific Inc. and its subsidiaries.
269-165900, Rev. B

Contents
Introduction.........................................................................................1
Conventions used in this manual .............................................................. 2
Questions or concerns............................................................................... 3
Spectrophotometer Basics ....................................................................4
Spectrophotometer components................................................................ 4
Connectors ............................................................................................ 5
Accessories............................................................................................8
Manual accessories .................................................................................... 8
Installation............................................................................................. 9
Standard rectangular cell holder ........................................................... 10
Adjustable micro cell holder.................................................................10
Long pathlength rectangular cell holder ............................................... 12
Cylindrical cell holder.......................................................................... 13
Thermostatted single cell holder .......................................................... 13
Test tube/vial holder ............................................................................ 15
Rectangular cell holder......................................................................... 15
Fiber optic sampling accessory ............................................................. 16
DRA-EV-600 Diffuse Reflectance accessory ........................................18
Solid Sample Holder............................................................................ 19
Smart Accessories .................................................................................... 20
System accessories ................................................................................... 21
Installing Smart Accessories..................................................................... 22
Using the Instrument .........................................................................25
Local Control.......................................................................................... 25
Main Menu page .................................................................................27
Text entry ............................................................................................ 37
Editing numeric fields..........................................................................38
Pop-up menus...................................................................................... 39
File operation....................................................................................... 39
Scan application................................................................................... 41
Fixed application.................................................................................. 45
Quantitation application...................................................................... 48
Kinetics application.............................................................................. 50
Multicomponent application ............................................................... 54
Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide i

ii Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific
Biological tests .....................................................................................56
Using UVcalc .......................................................................................68
Instrument verification tests .................................................................71
VISIONsecurity and VISIONpro.............................................................74
Maintenance.......................................................................................75
Routine maintenance ..............................................................................75
Cleaning instrument exterior...................................................................75
Internal printer........................................................................................76
Light sources (Evolution 600 only) .........................................................78
Deuterium lamp ..................................................................................80
Tungsten-halogen lamp .......................................................................81
Mercury lamp (Evolution 300 and Evolution 600) ..............................82

Introduction
Congratulations on your purchase of an Evolution™ 300 or Evolution 600
spectrophotometer! Our spectrophotometers integrate advanced hardware
features with the power and flexibility of a wide range of Smart
Accessories™.
Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 1

Conventions used in this manual
This manual includes safety precautions and other important information
presented in the following format:
Note Notes contain helpful supplementary information. 
Notice Follow instructions labeled “Notice” to avoid damaging the system
hardware or losing data. 
Caution Indicates a hazardous situation which, if not avoided, could result in minor
or moderate injury. 
Warning Indicates a hazardous situation which, if not avoided, could result in death
or serious injury. 
Danger Indicates a hazardous situation which, if not avoided, will result in death or
serious injury. 
2 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific

The following safety symbols are used on this product:
Symbol Description Indication
Black graphical
symbol inside a
yellow triangle with a
black triangular band
This is a warning symbol. The graphic in
this symbol is used to alert the user to
potential hazards.
Black graphical
symbol inside a red
circular band with a
red diagonal bar
This is a prohibition symbol. The graphic in
this symbol is used to alert the user to
actions that shall not be taken or shall be
stopped.
White graphical
symbol inside a blue
circle
This is a mandatory action symbol. It is
used to indicate that an action shall be
taken to avoid a hazard.
Black graphical
symbol inside a
yellow triangle with a
black triangular band
This is the general warning sign. Failure to
heed the safety precautions could result
in personal injury.
White graphical
symbol inside a blue
circle
This is the general data loss or property
damage symbol and is not related to
personal injury. Failure to heed these
precautions can result in irreparable
damage to property or permanent data
loss.
Questions or concerns
In case of emergency, follow the procedures established by your facility. If
you have questions or concerns about safety or need assistance with
operation, repairs or replacement parts, you can contact our sales or service
representative in your area or use the information at the beginning of this
document to contact us.
Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 3

Spectrophotometer Basics
This chapter describes the major components of your spectrophotometer.
Spectrophotometer components
The following illustration identifies some major components visible on the
outside of a typical Evolution spectrophotometer. (Some components may
not be present on your spectrophotometer.)
Contrast
Keypad
LCD screen
Zero
Run
789
456
123
0
.-
Optional printer
Floppy di
sk
drive
Sliding door
Sample
compartment
P
ort for optional
power supply
4 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific

Connectors
The following illustrations show the locations of the connectors on each
side of the spectrophotometer.
Right side
Zero
Run
789
456
123
0
.-
PRINTER KEY BD EXT ACCY RS232 DISPLAY
EXT
Connector Function
Printer Use to connect a printer with a parallel interface.
Key Bd Use to connect an English keyboard (this is supplied by
us to meet electrical emission requirements).
Ext Use to connect an external TTL (or contact closure)
trigger.
Accy Use to connect an external accessory.
RS232 Use to connect a computer with an RS232 interface.
Ext Display Use to connect to a computer monitor.
Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 5

Left side
Port for optional
Peltier power supply
Power ON/OFF switch
Power supply
connection
Power cord
Zero
Run
789
456
123
0
.-
Danger Avoid shock hazard. Always power off the spectrophotometer and
disconnect the power supply from the wall outlet or power strip before
disconnecting the power supply from the spectrophotometer. 
Inside sample compartment
The following illustrations show the locations of the connectors inside the
sample compartment.
Evolution 300
Reference
connector
Sample
connector
6 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific