Thermo Scientific Finnpipette FOCUS User manual

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Product specifications are subject to change without prior notice. Finnpipette®and
Finntip®are registered trademarks of Thermo Scientific.
See the latest version at www.thermo.com/finnpipette
Änderung von Produktbeschreibungen ist vorbehalten ohne vorherige Benachrichtigungt.
Finnpipette®und Finntip®sind eingetragene Warenzeichen von Thermo Scientific.
Sehen Sie die neueste Version an www.thermo.com/finnpipette
Las especificaciones del producto están sujetas a cambio sin notificación previa.
Finnpipette®y Finntip®son marcas registradas de Thermo Scientific.
La última versión está disponible en www.thermo.com/finnpipette
Les spécifications des produits peuvent être modifiées sans préavis. Finnpipette®et
Finntip®sont des marques déposées de Thermo Scientific.
Voir la dernière version sur www.thermo.com/finnpipette
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English
CONTENTS
PRODUCT DESCRIPTION 4
PIPETTE OPERATION 5
PIPETTING TECHNIQUES 5
CALIBRATION AND ADJUSTMENT 7
MAINTENANCE 9
TROUBLE SHOOTING 11
PACKAGE 11
SPARE PARTS 48-54
Deutsch
Français
Español
SOMMAIRE
DESCRIPTION DU PRODUIT 21
UTILISATION DE LA PIPETTE 22
METHODES DE PIPETAGE 22
CALIBRAGE ET RÉGLAGE 23
ENTRETIEN 26
CONDITIONNEMENT 29
EN CAS DE PROBLEME 29
PIÈCES DÉTACHÉES 48-54
CONTENIDO
DESCRIPCIÓN DEL PRODUCTO 30
USO DE LA PIPETA 31
TÉCNICAS DE PIPETEO 31
CALIBRACIÓN Y AJUSTE 32
MANTENIMIENTO 35
PAQUETE 37
SOLUCIÓN DE PROBLEMAS 38
PIEZAS DE RECAMBIO 48-54
INHALT
PRODUKTBESCHREIBUNG 12
PIPETTENFUNKTION 13
PIPETTIERMETHODEN 13
KALIBRIERUNG UND JUSTIERUNG 15
WARTUNG 17
PACKUNG 20
FEHLERBEHEBUNG 20
ERSATZTEILE UND ZUBEHÖR 48-54
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Product description
The Finnpipette FOCUS is an autoclavable pipette. It operates on the air displacement
principle and uses detachable, disposable tips.
The adjusted delivery volume is displayed on a numeric readout window on the handle.
The eleven different models of Finnpipette FOCUS pipettes with short tip cones cover a
volume range from 0.3 µl to 1000 µl.
The thirteen different models of Finnpipette FOCUS pipettes with standard tip cones cover
a volume range from 0.3 µl to 10 ml.
The sixteen different models of Finnpipette FOCUS Fixed Volume pipettes with short tip
cones cover a volume range from 1 µl to 10 ml.
The twelve different models of Finnpipette FOCUS Fixed Volume pipettes with standard tip
cones cover a volume range from 1 µl to 1000 µl.
Numeric display
The adjusted delivery volume is clearly indicated in the numeric display on the handle.
Raw materials
The Finnpipette FOCUS is made of mechanically durable and chemically resistant materials
which allow repeated autoclaving of the complete pipette at 121°C (252°F).
The user should verity that the pipette and tip is resistant to the liquid which it is intended to be used.
Description of tips
Finntips are recommended for use with the Finnpipette FOCUS.
They are made of natural colour polypropylene, generally regarded as the only contamination
free material suitable for tips. Finntips are also autoclavable (121°C / 252°F).
Order No.
Short Standard Volume Range Finntip
4600100 4600150 0,3 µl to 3 µl 10
4600110 4600160 0,5 µl to 5 µl 10
4600080 4600170 1 µl to 10 µl 10
4600010 4600180 1 µl to 10 µl 250 Universal, 200 Ext
4600120 4600190 3 µl to 30 µl 50
4600130 4600200 3 µl to 30 µl 250 Universal, 200 Ext
4600090 4600210 5 µl to 50 µl 50
4600020 4600220 5 µl to 50 µl 250 Universal, 200 Ext
4600030 4600230 10 µl to 100 µl 250 Universal, 200 Ext
4600040 4600240 30 µl to 300 µl 300
4600050 4600250 100 µl to 1000 µl 1000
- 4600400 0,5 ml to 5 ml 5 ml
- 4600410 1 ml to 10 ml 10 ml
Order No.
Short Standard Volume Range Finntip
4601000 4601160 1 µl 10
4601010 4601170 2 µl 10
4601020 4601180 5 µl 10
4601030 4601190 10 µl 10
4601040 4601200 20 µl 250 Universal, 300, 200 Ext
4601050 4601210 25 µl 250 Universal, 300, 200 Ext
4601060 4601220 50 µl 250 Universal, 300, 200 Ext
4601070 4601230 100 µl 250 Universal, 300, 200 Ext
4601080 4601240 200 µl 250 Universal, 300, 200 Ext
4601090 4601250 250 µl 250 Universal, 300
4601100 4601260 500 µl 1000
4601110 4601270 1000 µl 1000
4601120 - 2 ml 5 ml
4601130 - 3 ml 5 ml
4601140 - 5 ml 5 ml
4601150 - 10 ml 10 ml
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Pipette operation
Setting the delivery volume
1. Rapid adjustment: Set the delivery volume using the push
button on the top of the pipette. To increase the delivery
volume, turn the push button counterclockwise.
To decrease the delivery volume, turn it clockwise.
2. Fine adjustment: Set the last digit (0-9) of the delivery
volume by turning the fine adjustment ring.
3. Make sure that the desired delivery volume clicks into place
and that the digits are completely visible in the display window.
The adjustable volume is limited to the specific volume range.
Using excessive force to turn the push button outside the range may jam
the mechanism and eventually damage the pipette.
Tip ejection
To help eliminate the risk of contamination, each pipette is fitted with a tip
ejector system.
The tip ejector system consists of a soft-touch tip ejector and specially
designed gearing mechanism. To release the tip, point the pipette at suitable
waste receptacle and press the tip ejector with your thumb.
Shelf hanger
You can attach the pipette shelf hanger on a counter, pipette stand or
anywhere where you want to hang your pipette.
Clean the area where you plan to attach the shelf hanger. Apply two stickers
to the underside of the shelf hanger. Press the shelf hanger firmly into place
— on a shelf, counter top or pipette stand. To use, hang the grippy finger rest
on the shelf hanger.
Pipetting techniques
General
Special care shall be taken to avoid liquid being aspirated into the interior
working of the pipette.
Push and release the push button slowly at all times, particularly when working
with high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 3-5 times with the
solution that you will be pipetting. Hold the pipette in an upright position while aspirating
liquid. The grippy should rest on your index finger. Make sure that the tips, pipette and
solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the liquid by gently depressing the
push button to the first stop. After a delay of
about one second, continue to depress the
push button all the way to the second stop.
This action will empty the tip.
4. Release the push button to the ready position.
If necessary, change the tip and continue pipetting.
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Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very small volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
This action will fill the tip.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push button at the first stop.
Some liquid will remain in the tip and this should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into
the container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the
same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
This action will fill the tip.
Withdraw the tip from the liquid touching
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push button at the first stop. Some liquid will
remain in the tip and this should not be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with sample.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress
the push button to the first stop, making sure
the tip is well below the surface.
2. Release the push button slowly to the ready
position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly.
Keep repeating this procedure until the
interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty
the tip.
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Calibration and adjustment
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that
the use of other pipetting techniques may affect the calibration results. The pipettes are
constructed to permit re-adjustment for other pipetting techniques or liquids of different
temperature and viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be
chosen according to the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.00 1 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are
done in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air
between 15°C to 30°C.
The relative humidity must be above 50%. Especially with volumes under 50 µl the air
humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed
for forward method.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
TABLE 1: Maximum permissible errors according ISO8655
Range Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
0,3-3 µl 3 ±0.125 ±4.2 0.075 2.5
0,3 ±0.125 ±42 0.075 25
0,5-5 µl 5 ±0.125 ±2.5 0.075 1.5
0,5 ±0.125 ±25 0.075 15
1-10 µl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
3-30 µl 30 ±0.50 ±1.7 0.20 0.7
3 ±0.50 ±17 0.20 6.7
5-50 µl 50 ±0.50 ±1.0 0.20 0.4
5 ±0.50 ±10 0.20 4.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
30-300 µl 300 ±4.0 ±1.3 1.5 0.5
30 ±4.0 ±13 1.5 5.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
0,5-5 ml 5000 ±40.0 ±0.8 15.0 0.3
500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3
1000 ±60.0 ±6.0 30.0 3.0
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Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1 ±0.04 ±4.0 0.04 4.0
2 ±0.05 ±2.5 0.04 2.0
5 ±0.07 ±1.4 0.07 1.4
10 ±0.09 ±0.9 0.08 0.8
20 ±0.12 ±0.6 0.10 0.5
25 ±0.15 ±0.6 0.13 0.5
50 ±0.3 ±0.6 0.2 0.4
100 ±0.4 ±0.4 0.3 0.3
200 ±0.8 ±0.4 0.6 0.3
250 ±1.0 ±0.4 0.8 0.3
500 ±1.5 ±0.3 1.5 0.3
1000 ±3.0 ±0.3 0.3 0.3
2 ml ±6.0 ±0.3 4.0 0.2
3 ml ±9.0 ±0.3 6.0 0.2
5 ml ±15.0 ±0.3 10.0 0.2
10 ml ±30.0 ±0.3 20.0 0.2
Adjustment
Adjustment is done with the service tool.
1. Place the service tool into the openings of the
calibration nut at the top of the handle. Keep fine
volume adjustment in place.
2. Turn the service tool clockwise to increase, or
counterclockwise to decrease the volume.
3. After adjustment check the calibration according
to the instructions above.
Formulas for calculating results
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense
water to the weighing vessel, note the reading and start a stopwatch. See how much the
reading decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be
10 seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap
or lid on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and
pressure. A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on
page 47.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V0A = inaccuracy
V = mean volume
V0= nominal volume
Inaccuracy can be expressed as a relative value:
A% = 100% x A / V0
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation
(s) or coefficient of variation (cv)
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s = standards deviation
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S
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v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV) CV = 100% x S / V
Maintenance
When the Finnpipette FOCUS is not in use, make sure it is stored in an upright position. We
recommend a Finnpipette stand for this purpose.
The part # refer to exploded views beginning at page 48 and 52 Standard model.
Daily checking
The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette. Particular attention should be paid to the tip cone. No other
solvents except 70 % ethanol should be used to clean the pipette.
Short-term service
If the pipette is used daily it should be checked and lubricated every three months.
Pipettes up to 1000 µl
1. Remove the lower tip ejector sleeve 13 by pulling it out (snap fitting).
2. Push down the ejector button and take the upper ejector top 11
between fingers.
3. Release the ejector button to up position, but hold the tip ejector top
11 in down position with the fingers.
4 Remove the tip cone by pulling it out (snap fitting).
5. Pull out the piston 14 and the spring 15 but not spring 16.
6. Clean the piston 14 and the piston spring 15 with a dry napless cloth.
7. Check the tip cone for foreign particles.
8. Grease the cleaned parts with the lubricant that comes with the pipette.
9. Reassemble the pipette components in reverse order.
5 ml and 10 ml pipettes / 2-10 ml pipettes
1. Pull down the tip ejector sleeve.
2. Note that the tip ejector button is in fully up position.
3. Remove the tip ejector by pulling it out.
4. Remove the ejector sleeve by pulling it to two parts
(snap fitting).
5. Remove the cylinder 14 by pushing the piston to the
down position and push the snap joints to release and
remove the cylinder 14.
6. Push the piston forward and clean it with a dry
napless cloth.
7. Check the tip cone for foreign particles.
8. Grease the cleaned parts with the lubricant that comes
with the pipette.
9. Reassemble the pipette components in reverse order.
Long-term service
If the pipette is used daily it should be serviced every six months.
Pipettes up to 1000µl
1. Remove the lower tip ejector sleeve 13 by pulling it out (snap fitting).
2. Push down the ejector button and take the upper ejector top 11 between fingers.
3. Release the ejector button to up position, but hold the tip ejector top 11 in down
position with the fingers.
4. Remove the tip cone by pulling it out (snap fitting).
5. Pull out the piston 14 and the spring 15.
6. Remove spring hatch 25 by pressing it down and in the same time turning 90 degrees.
Take out the spring 16.
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7. Remove the rest of the parts from tip cone with the piston.
8. Clean the parts with a dry napless cloth.
9. Check the tip cone for foreign particles.
10. Grease the cleaned parts with the lubricant that comes with the pipette.
5 ml and 10 ml pipettes / 2-10 ml pipettes
The long-term service for 5ml and 10ml pipettes is the same as short-term service.
Reassembly:
1-10 µl, 0.5-5 µl and 0.3-3 µl pipettes:
1. Put the spring hatch 25, the spring 16, spring support 17, (part 26 only standard model)
and the tube 18 back on the piston.
2. Slide the bigger O-ring 19, smaller O-ring 20 and tube 21 on the piston.
3. Slide the small spring 22, spring support 23 and O-ring 24 on the tube 21.
4. Carefully slide the entire assembly into the tip, then push the spring hatch 25 down and
turn it 90 degrees.
5. Pull out the piston, insert the spring 15 on piston, and put the piston back into the tip cone.
6. Take the upper ejector top 11 between fingers and pull it down.
7. Push the tip cone into the handle while holding the ejector top 11 down.
8. Reassemble the lower tip ejector sleeve 13 (snap fitting).
5-50 µl and 3-30 µl pipettes / 20-50 µl pipettes:
1. Put the spring hatch 25, the spring 16, spring support 17 and the tube 18 back on
the piston.
2. Slide the bigger O-ring 19, smaller O-ring 20 and O-ring support 21 on the piston.
3. Slide the small spring 22 on the piston.
4. Carefully slide the entire assembly into the tip, then push the spring hatch 25 down and
turn it 90 degrees.
5. Pull out the piston, insert the spring 15 on piston, and put the piston back into the
tip cone.
6. Take the upper ejector top 11 between fingers and pull it down.
7. Push the tip cone into the handle while holding the ejector top 11 down.
8. Reassemble the lower tip ejector sleeve 13 (snap fitting).
10-100µl pipette / 100 µl pipettes:
1. Put the spring hatch 25, the spring 16, spring support 17 and the O-ring 20 back on
the piston.
2. Carefully slide the entire assembly into the tip, then push the spring hatch 25 down and
turn it 90 degrees.
3. Pull out the piston, insert the spring 15 on piston, and put the piston back into the
tip cone.
4. Take the upper ejector top 11 between fingers and pull it down.
5. Push the tip cone into the handle while holding the ejector top 11 down.
6. Reassemble the lower tip ejector sleeve 13 (snap fitting).
30-300µl pipette / 200-300 µl pipettes:
1. Put the spring hatch 25, the spring 16, spring support 17 and the O-ring 20 back on
the piston.
2. Carefully slide the entire assembly into the tip, then push the spring hatch 25 down and
turn it 90 degrees.
3. Pull out the piston, insert the spring 15 on piston, and put the piston back into the
tip cone.
4. Take the upper ejector top 11 between fingers and pull it down.
5. Push the tip cone into the handle while holding the ejector top 11 down.
6. Reassemble the lower tip ejector sleeve 13 (snap fitting).
100-1000µl pipette / 500-1000 µl pipettes:
1. Put the spring hatch 25, the spring 16, spring support 17 and the O-ring 20 back on
the piston.
2. Put the spring 15 on the piston.
3. Carefully slide the entire assembly into the tip cone.
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