Thermo Scientific TRACE 1600 User manual

Thermo Scientific
TRACE 1600/1610
Hardware Manual
Gas Chromatographs
MI-317000-0033 Revision A April 2022

© 2022 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Scientific™ TRACE™ 1600, and TRACE™1610, are trademarks of Thermo Fisher Scientific.
Microsoft® is a registered trademark of Microsoft. Adobe® is a registered trademark of Adobe Systems
Incorporated in the United States and/or other countries. Swagelok® is a registered trademark of Swagelok
Company. Viton® and Vespel® are registered trademark of DuPont. SilFlow®, NoVent®, and FingerTite® are
registered trademarks of Trajan Scientific and Medical. All other trademarks are the property of Thermo Fisher
Scientific and its subsidiaries.
Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana, km 4, 20090 Rodano-Milan, Italy
Tel: [phone]
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history:
First Edition, April 2022- “Original Instructions”
General Lab Equipment. Not for Clinical, Patient, or Diagnostic Use.

fold
fold
Reader’s Survey
TRACE 1600/1610 Hardware Manual, MI-317000-0033, Revision A
If not, please comment below. Attach additional sheets if necessary.
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
Customer Registration Card
Register now…and receive all the privileges associated with being a Thermo Fisher Scientific product user including customer
support, application reports, and technical reports.
Name __________________________________________________Title__________________________________________________________________
Company __________________________________________________ __________________________________________________________________
Address ___________________________________________________ __________________________________________________________________
City/State _________________________________________ Postal Code__________________________________________________________________
Country ___________________________________________________ __________________________________________________________________
Telephone_______________________________________________ Ext. __________________________________________________________________
Serial Number __________________________________ Date purchased __________________________________________________________________
Fold and mail or e-mail to:
Strongly
Agree Agree Neutral Disagree Strongly Disagree
The manual is well organized. 1 2 3 4 5
The manual is clearly written. 1 2 3 4 5
The manual contains all the information I need. 1 2 3 4 5
The instructions are easy to follow. 1 2 3 4 5
The instructions are complete. 1 2 3 4 5
The technical information is easy to understand. 1 2 3 4 5
Examples of operation are clear and useful. 1 2 3 4 5
The figures are helpful. 1 2 3 4 5
I was able to operate the system using this manual. 1 2 3 4 5
MY ORGANIZATION IS: (Check only one) MY PRIMARY APPLICATION IS: (Check only one)
❏Commercial (for profit) lab ❏Analytical
❏Government lab ❏Biomedical
❏Hospital/Clinic ❏Clinical/Toxicology
❏Industrial lab ❏Energy
❏Research Institute ❏Environmental
❏University/College ❏Food/Agricultural
❏Veterinary ❏Forensic/Toxicology
❏Other______________________ ❏Pharmaceutical
❏Research/Education
MY PRIMARY JOB FUNCTION IS: (Check only one) ❏Other______________________
❏Administration
❏Lab management
❏Operator
❏Other______________________
Editor, Technical Publications
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, km 4
20090 Rodano (MI)
Italy
Editor, Technical Publications
Thermo Fisher Scientific GC-GC/MS
2215 Grand Avenue Parkway
Austin TX 78728-3812
Unites States of America

Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
• installation
• re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, km 4, 20090 Rodano - Milan - Italy — Tel: [phone] - Fax: [phone]
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
Thermo Fisher Scientific declares, under sole responsibility, that the product as originally delivered complies with
the requirements of the following applicable European Directives and carries the CE marking accordingly:
• Low Voltage Directive:2014/35/EU
• EMC Directive:2014/30/EU
• RoHS Directive 2011/65/EU, including amending Delegate Directive 2015/863/EU
… and conforms with the following product standards:
Safety
This device complies with:
• IEC 61010-1:2010/AMD1:2016, IEC 61010-2-010:2019
• CAN/CSA C22.2 No. 61010-1
• EN 61010-1:2020, EN 61010-2-010:2015
• UL 61010-1
Electromagnetic Compatibility
This device complies with:
• CISPR 11/EN 55011: Group 1 Class A
• IEC 61326-1:2012
• EN 61326-1:2012
• FCC part 15, Subpart B, §15.107(a) and §15.109(a)

FCC Compliance Statement
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
IMPORTANT: Class A equipment is intended for use in an industrial environment. In others
environments there may be potential difficulties in ensuring electromagnetic compatibility, due
to the conducted as well as radiated disturbances.
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

Declaration of Conformity

China EEP Hazardous Substances Information

WEEE Directive
2012/19/EU
Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the
European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway.
If this product is located in Europe and you want to participate in the Thermo Fisher Scientific Business-to-Business
(B2B) Recycling Program, send an email request to [e-mail] with the following information:
• WEEE product class
• Name of the manufacturer or distributor (where you purchased the product)
• Number of product pieces, and the estimated total weight and volume
• Pick-up address and contact person (include contact information)
• Appropriate pick-up time
• Declaration of decontamination, stating that all hazardous fluids or material have been removed from the product
For additional information about the Restriction on Hazardous Substances (RoHS) Directive for the European Union,
search for RoHS on the Thermo Fisher Scientific European language websites.
IMPORTANT This recycling program is not for biological hazard products or for products that have been medically
contaminated. You must treat these types of products as biohazard waste and dispose of them in accordance with
your local regulations.

Directive DEEE
2012/19/EU
Thermo Fisher Scientific s'est associé avec une ou plusieurs sociétés de recyclage dans chaque état membre de l’Union
Européenne et ce produit devrait être collecté ou recyclé par celle(s)-ci. Pour davantage d'informations, rendez-vous sur
la page www.thermoscientific.fr/rohs.
WEEE Direktive
2012/19/EU
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten
getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Weitere Informationen
finden Sie unter www.thermoscientific.de/rohs.

Thermo Scientific TRACE 1600/1610 Hardware Manual xi
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Power Rating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxiii
Hydrogen Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Using Hydrogen with TRACE 1600/TRACE 1610 . . . . . . . . . . . . . . . . . . xxv
Hydrogen Connection Guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Purchasing Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvii
Properly Storing Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxviii
Hydrogen Safety Codes, Standards and References . . . . . . . . . . . . . . . . . . . xxix
Hazardous Substances Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxx
Venting Toxic Gases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxx
Liquid Nitrogen Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxx
Carbon Dioxide Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxxi
Chapter 1 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Positioning the TRACE 1600/1610 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Installing External Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installing the Injector and Detector Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installing an Injector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Installing a Detector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Making the Gas Supply Plumbing Connections . . . . . . . . . . . . . . . . . . . . . . . . . 8
Testing for Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Connecting the Oven Cryogenic System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Oven Cryo System with Carbon Dioxide Connection. . . . . . . . . . . . . . . . . . 14
Oven Cryo System with Liquid Nitrogen Connection. . . . . . . . . . . . . . . . . . 16
Connecting the PTV/PTVBKF Cryogenic System . . . . . . . . . . . . . . . . . . . . . . 19
PTV/PTVBKF Cryo System with Carbon Dioxide Connection . . . . . . . . . . 19
PTV/PTVBKF Cryo System with Liquid Nitrogen Connection . . . . . . . . . . 22
Contents

Contents
xii TRACE 1600/1610 Hardware Manual Thermo Scientific
Coupling to a Mass Spectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Making the Duct Couple with an ISQ Series or TSQ Series Mass
Spectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Making the Duct to Couple with a DFS, IRMS, or ICP-MS Mass
Spectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Setting Handshake Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Installing the Autosampler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Mounting an Autosampler on the GC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Connecting the AI/AS 1610 in Standard Configuration . . . . . . . . . . . . . . . . 33
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Instrument Start-up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Connecting TriPlus 500 HS, TriPlus RSH SMART, or TriPlus 100
Autosampler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Setting Autosampler Handshake Parameters . . . . . . . . . . . . . . . . . . . . . . . . . 36
Installing the Data System Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Making Power Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Setting the LAN Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Making the LAN Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Configuring the Data System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Column Installation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Using the Correct Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Installing the Adapters for Encapsulated Graphite Ferrules . . . . . . . . . . . . . . 48
Installing the Column Rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Installing the Column the First Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
iConnect Column Lock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Chapter 2 Performing Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
Read Me First . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Maintenance Supplies and Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Cleaning Stainless Steel Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Maintenance Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Powering On the TRACE 1600/1610. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Powering Off the TRACE 1600/1610. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Cleaning the Instrument Externally. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Replacing a Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Chapter 3 Performing Injectors Routine Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Maintaining a Split/Splitless Injector (SSL) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Replacing the SSL Septum. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Cleaning or Replacing the SSL Glass Liner . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Replacing a SSL Broken Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Replacing the SSL Carrier and Split Lines Filters. . . . . . . . . . . . . . . . . . . . . . 93
Replacing the SSL Body Head O-rings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Contents
Thermo Scientific TRACE 1600/1610 Hardware Manual xiii
Maintaining a Split/Splitless Injector with Backflush (SSLBKF) . . . . . . . . . . . . 97
Replacing the SSLBKF Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Cleaning or Replacing the SSLBKF Glass Liner. . . . . . . . . . . . . . . . . . . . . . 102
Replacing a SSLBKF Broken Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Replacing the SSLBKF Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . 106
Replacing the SSLBKF Body Head O-Rings . . . . . . . . . . . . . . . . . . . . . . . . 108
Maintaining a Gas Sampling Valve Injector (GSV) . . . . . . . . . . . . . . . . . . . . . 110
Connecting the Sample In and Out Lines . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Replacing the Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . . . . . . . 113
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Replacing the Sample Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL). . . . . . . . 116
Replacing the Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Cleaning or Replacing the Glass Liner. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Replacing the Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . . . . . . . 122
Replacing the Body Head O-rings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Maintaining a Programmable Temperature Vaporizing Injector (PTV). . . . . . 126
Replacing the PTV Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Cleaning or Replacing the PTV Glass Liner . . . . . . . . . . . . . . . . . . . . . . . . 130
Replacing the PTV Broken Liner. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Replacing the PTV Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . . . 135
Maintaining a Programmable Temperature Vaporizing Injector with
Backflush (PTVBKF) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Replacing the PTVBKF Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
Cleaning or Replacing the PTVBKF Glass Liner . . . . . . . . . . . . . . . . . . . . . 141
Replacing the PTVBKF Broken Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Replacing the PTVBKF Carrier and Split Lines Filters . . . . . . . . . . . . . . . . 146
Chapter 4 Performing Detectors Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149
Maintaining a Flame Ionization Detector (FID) . . . . . . . . . . . . . . . . . . . . . . . 150
Cleaning or Replacing the FID Collecting Electrode . . . . . . . . . . . . . . . . . . 153
Replacing the FID Ignition Glow-plug . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
Maintaining a Nitrogen Phosphorous Detector (NPD). . . . . . . . . . . . . . . . . . 159
Replacing the NPD Thermionic Source . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
Cleaning or Replacing the NPD Collecting Electrode . . . . . . . . . . . . . . . . . 169
Maintaining a Thermal Conductivity Detector (TCD) . . . . . . . . . . . . . . . . . . 177
Bake-out Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Measuring the Carrier Gas Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Shutting Down the TCD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Maintaining an Electron Capture Detector (ECD) . . . . . . . . . . . . . . . . . . . . . 180
Detector Chemical Contamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
Wipe Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Cleaning or Replacing the Collecting Electrode (Anode). . . . . . . . . . . . . . . 183

Contents
xiv TRACE 1600/1610 Hardware Manual Thermo Scientific
Maintaining a Flame Photometric Detector (FPD) . . . . . . . . . . . . . . . . . . . . . 186
Installing the FPD Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190
Removing the FPD Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192
Cleaning or Replacing the FPD Jet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
Cleaning or Replacing the FPD Interferential Filter . . . . . . . . . . . . . . . . . . 197
Replacing the FPD Ignition Glow-plug. . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Maintaining a Pulsed Discharge Detector (PDD) . . . . . . . . . . . . . . . . . . . . . . 207
Chapter 5 GC Main Frame Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .209
Removing/Replacing the GC Top Cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210
Removing/Replacing the GC Left Side Panel . . . . . . . . . . . . . . . . . . . . . . . . . 211
Removing/Replacing the GC Right Side Panel . . . . . . . . . . . . . . . . . . . . . . . . 213
Removing/Replacing the GC Back Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215
Removing/Replacing the GC Front Door Cover . . . . . . . . . . . . . . . . . . . . . . . 217
Removing/Replacing the Electronic Module . . . . . . . . . . . . . . . . . . . . . . . . . . 222
Replacing the Oven Heater Baffle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225
Replacing the Oven Heater Temperature Sensor . . . . . . . . . . . . . . . . . . . . . . . 229
Replacing the Oven Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 233
Replacing the Flap Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239
Chapter 6 Injectors Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .243
Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and
PTVBKF Injectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
Removing/Replacing an Injector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
Cleaning the SSL Injector Body. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 248
Cleaning the SSLBKF Injector Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252
Cleaning the HeS-S/SL Injector Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
Cleaning the PTV Injector Head Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . 260
Cleaning the PTVBKF Injector Head Assembly . . . . . . . . . . . . . . . . . . . . . . . 268
Chapter 7 Detectors Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .277
Removing/Replacing a Detector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 277
Measuring the FID Gas Flows. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 280
Cleaning or Replacing the FID Jet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
Measuring the NPD Gas Flows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 291
Cleaning or Replacing the NPD Jet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 301
Measuring the FPD Gas Flows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 309
Cleaning or Replacing the FPD Mirror Metal Plug . . . . . . . . . . . . . . . . . . . . . 311
Cleaning or Replacing the FPD Filter-side Heat Shields . . . . . . . . . . . . . . . . . 316
Cleaning or Replacing the FPD Flame-side Heat Shields. . . . . . . . . . . . . . . . . 324
Replacing the FPD Photomultiplier Tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . 334
Chapter 8 Installing Optional Kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .345
Installing the Oven Exhaust Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 346

Contents
Thermo Scientific TRACE 1600/1610 Hardware Manual xv
Installing the Merlin Microseal High Pressure Valve Kit . . . . . . . . . . . . . . . . . 347
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 347
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 347
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector . . . . . . 352
Installing the Packed Column Adapters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 359
Installing the HS Adapter Kit on the SSL/SSLBKF Injector . . . . . . . . . . . . . . 365
Installing the Large Volume Splitless Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 376
Installing the Manual On/Off Valve for Single Gas Line. . . . . . . . . . . . . . . . . 379
Connecting a SSL/PTV Backflush System. . . . . . . . . . . . . . . . . . . . . . . . . . . . 382
Connecting a SSL/PTV Backflush System for High Temperature . . . . . . . . . . 390
Connecting a GSV Backflush System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 396
Installing the NoVent Microfluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399
Connecting the NoVent Microfludics Module to the TRACE
1600/1610 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399
Installing the Mounting Bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 402
Preparing the NoVent Microfluidics Restrictor Tubing. . . . . . . . . . . . . . . . 405
Attaching the Ferrule and Nut to the GC Column . . . . . . . . . . . . . . . . . . . 408
Attaching the New Tubing to the Transfer Line . . . . . . . . . . . . . . . . . . . . . 410
Connecting the Capillaries to the Microfluidics Splitter . . . . . . . . . . . . . . . 414
Configuring the Post-Column. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 416
Using the Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 418
Installing a FTIR Make-up Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 420
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector . . . . . . 424
Performing the Dual FPD Detector Configuration . . . . . . . . . . . . . . . . . . . . . 433
Installing the Pressure Regulator Kit for Gas Sampling Valve . . . . . . . . . . . . . 444
Installing the Packed Columns Support Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . 447
Installing the ECD Exhaust Vent Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 452
Chapter 9 Adding Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .455
Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module . . . . . . . . . . . . . 456
Adding a GSV Injector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 459
Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module . . . . . . 465
Adding a NPD Detector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 469
Adding an Aux Temperature/Cryo Module. . . . . . . . . . . . . . . . . . . . . . . . . . . 478
Adding a Helium Saver Injector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 483
Installing the Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 483
Installing the Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 489
Checking for Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 491

Adding a PDD Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 493
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 493
Installing a PDD Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 494
Plumbing the Gas Lines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 498
Connecting the High Voltage and Pulses Cables . . . . . . . . . . . . . . . . . . . . . 500
Installing the Capillary Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 505
Installing the Packed Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 505
Testing for Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 505
Performing Initial Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 506
Adding a Generic Detector Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 507
Preliminary Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 507
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 507
Removing the GC Back Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 508
Assembling the GDI Electrical Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . 509
Replacing the Encapsulated Flow Restrictors. . . . . . . . . . . . . . . . . . . . . . . . 511
Installing and Connecting the GDI Electrical Interface . . . . . . . . . . . . . . . . 512
Connecting a GDI Interface to the TRACE 1600 Auxiliary Oven. . . . . . . . 517
Installing a GDI Mechanical Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 518
Connecting the Detector Gas Tubing to the Manifolds. . . . . . . . . . . . . . . . 520
Connecting Heater and Signal Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 525
Restarting the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 526
Performing the Third-party Detector Start-up and Optimization . . . . . . . . 526
Configuring and Setting GDI Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . . 526
Adding an Analog Output Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 528
Chapter 10 Adding Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .533
Adding the Oven Cryo System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 534
Oven Cryo System Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 534
Installing the Oven Cryo System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 536
Adding the PTV and PTVBKF Cryo System. . . . . . . . . . . . . . . . . . . . . . . . . . 547
PTV/PTVBKF Cryo System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . 548
Installing the PTV/PTVBKF Cryo System . . . . . . . . . . . . . . . . . . . . . . . . . 549
Adding an Auxiliary Gas System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 558
Auxiliary Gas Module Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 559
Auxiliary Gas Interface Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 559
Preliminary Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 561
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 561
Installing the Auxiliary Gas Interface on the Right Wall of the Oven . . . . . 562
Installing the Auxiliary Gas Interface on the Oven for HRMS. . . . . . . . . . . 565
Installing and Connecting the Auxiliary Gas Module . . . . . . . . . . . . . . . . . 568
Adding the Hydrogen Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 576
Chapter 11 Upgrading Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .583
Upgrading a TRACE 1600 to a TRACE 1610 . . . . . . . . . . . . . . . . . . . . . . . . 584

Contents
Thermo Scientific TRACE 1600/1610 Hardware Manual xvii
Upgrading a Stand Alone TRACE 1600/1610 to MS Version. . . . . . . . . . . . . 590
Preliminary Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 590
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 590
Coupling with the ISQ Series or TSQ Series Mass Spectrometer. . . . . . . . . 590
Updating HMI Software From USB Stick. . . . . . . . . . . . . . . . . . . . . . . . . . . . 595
Chapter 12 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .599
Investigating Power Supply Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600
TRACE 1600/1610 will not power-on . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600
Investigating Communication Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600
Software is not communicating with the TRACE 1600/1610 . . . . . . . . . . 600
TRACE 1600/1610 does not start or is not ready . . . . . . . . . . . . . . . . . . . . 600
Cannot download methods to the TRACE 1600/1610 . . . . . . . . . . . . . . . 600
Sample data are not acquired. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 601
GC is not communicating with the PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . 601
Autosampler is not communicating with the PC . . . . . . . . . . . . . . . . . . . . . 601
Investigating Sensitivity Issues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 601
Poor sensitivity or sudden loss in sensitivity. . . . . . . . . . . . . . . . . . . . . . . . . 601
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 602
TRACE 1600 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 602
TRACE 1610 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 602
Contacting Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 607
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .609

Thermo Scientific TRACE 1600/1610 Hardware Manual xix
P
Preface
This manual contains detailed information about installing, maintaining, and
troubleshooting the Thermo Scientific™ TRACE™ 1600 and TRACE™ 1610 gas
chromatographs.
About Your System
Thermo Scientific systems provide high-caliber gas chromatography (GC) instrumentation.
Your TRACE 1600/TRACE 1610 GC system can be a stand-alone unit or coupled with other
instruments. GC represents a powerful analytical separation technique. Complex mixtures of
individual compounds can be injected into the GC, either manually or by using an
autosampler, and then separated the eluate for presentation to the detector. The detector
generates signals of the GC eluate and its components. These signals are then processed by a
Thermo Scientific™ Chromatography Data System for qualitative identification, as well as
accurate and precise quantification of the individual compounds present in the sample.
IMPORTANT Thermo Scientific systems optimize the separation and detection
capabilities of GC by providing high performance analytical capabilities for both research,
and routine applications. More information about the use of this system can be found in
related documentation sources, and by using the provided contact information.
WARNING Thermo Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment might be impaired. If
you maintain a system outside the specifications listed in this guide, failures of many
types, including personal injury or death, might occur. The repair of instrument failures
caused by operation in a manner not specified by the manufacturer is specifically excluded
from the standard warranty and service contract coverage.
WARNING Operation of this system requires the use of chemical substances with
different hazard specifications. Before using any chemicals, read the hazard indications
and information reported in the Safety Sheet supplied by the manufacturer, referring to
the relevant CAS (Chemical Abstract Service) number.

Preface
Power Rating
xx TRACE 1600/1610 Hardware Manual Thermo Scientific
Power Rating
TRACE 1600/TRACE 1610 gas chromatograph
• 120 Vac ±10%, 50/60 Hz, 2000 VA
• 230 Vac ±10%, 50/60 Hz, 2000 VA
Detailed instrument specifications are in the Product Specifications Sheet.
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
To find out more about our products
Go to http://www.thermofisher.com for information about our products.
To get local contact information for sales or service
Go to http://www.unitylabservice.com/en/home.html
Related Documentation
In addition to this guide, Thermo Scientific provides the following documents for the
TRACE 1600 and TRACE 1610.
TRACE 1600 /1610 Document Set, P/N MI-317AH0-0038
• TRACE 1600/1610 Preinstallation Requirements Guide, P/N MI-317000-0032
•TRACE 1600/1610 Hardware Manual, P/N MI-317000-0033
• TRACE 1600 /1610 User Guide, P/N MI-317000-0034
•TRACE 1600/1610 Spare Parts Guide, P/N MI-317000-0035