Thermo Scientific Finnpipette User manual

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Instructions for Use
Bedienungsanleitung
Guide d´utilisation et d´entretien
Instrucciones de uso
Digital Multichannel
Finnpipette®
3 0 0
Thermo Fisher Scientific
P.O.Box 100, Fin-01621 Vantaa, Finland
Tel. [phone], fax -358-9-3291 0414
www.thermo.com/finnpipette

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Finnpipette
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Finnpipette
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Finnpipette
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CONTENTS
PRODUCT DESCRIPTION 4
PIPETTE OPERATION 4
PIPETTING TECHNIQUES 5
CALIBRATION 6
MAINTENANCE 8
TROUBLE SHOOTING 10
PACKAGE 11
SPARE PARTS 39
ENGLISH
INHALT
PRODUKTBESCHREIBUNG 12
HANDHABUNG DER FINNPIPETTE 12
PIPETTIER-TECHNIKEN 13
KALIBRIEREN 15
WARTUNG 17
STÖRUNGEN BEHEBEN 19
VERPACKUNG 20
ERSATZTEILE UND ZUBEHÖR 39
DEUTSCH
SOMMAIRE
DESCRIPTION 21
UTILISATION DE LA PIPETTE 21
CONSEILS DE PIPETAGE 22
CALIBRATION 24
MAINTENANCE 26
ERREURS ET CAUSES 28
CONDITIONNEMENT 28
PIÈCES DÉTACHÉES 39
FRANÇAIS
CONTENIDO
DESCRIPCION DEL PRODUCTO 29
FUNCIONES 29
TECNICAS DE PIPETEO 30
CALIBRACION 31
MAINTENIMIENTO 33
PROBLEMAS Y SOLUCIONES 36
PRESENTACION 37
PIEZAS DE RECAMBIO 39
ESPAÑOL
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PRODUCT DESCRIPTION
The Finnpipette Digital Multichannel is an autoclavable digital pipette. It operates on the air
displacement principle (i.e. an air interface) and uses detachable, disposable tips.
The adjusted delivery volume is displayed digitally on a readout window on the handle.
The six different models of Finnpipette Digital Multichannel pipettes cover a volume range
from 0.5 µl to 300 µl.
Order No. Channel Volume Range Finntip
4510000 8 0.5 µl to 10 µl 10
4510010 12 0.5 µl to 10 µl 10
4510020 8 5 µl to 50 µl 250, 300
4510030 8 50 µl to 300 µl 250, 300
4510040 12 5 µl to 50 µl 250, 300
4510050 12 50 µl to 300 µl 250, 300
4510070 16 5 µl to 50 µl Finntip 50
DIGITAL DISPLAY
The adjusted delivery volume is clearly indicated in the large digital display on the
handle.
RAW MATERIALS
The Finnpipette Digital is made of mechanically durable and chemically resistant
materials which allow repeated autoclaving of the complete pipette at 121°C.
DESCRIPTION OF TIPS
Finntips are recommended for use with the Finnpipette Digital Multichannel.
They are made of natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
PIPETTEOPERATION
SETTING THE DELIVERY VOLUME
1. Set the delivery volume using the push button on the top of the pipette.
To increase the delivery volume, turn the push button counterclockwise.
To decrease the delivery volume, turn it clockwise.
2. Make sure that the desired delivery volume clicks into place and that the digits are
completely visible in the display window.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range may jam the mechanism
and eventually damage the pipette.
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TIP EJECTION
To help eliminate the risk of contamination, each pipette is fitted with a tip ejector system.
The tip ejector system consists of a soft-touch tip ejector and specially designed gearing
mechanism. To release the tip, point the pipette at suitable waste receptacle and press the
tip ejector with your thumb.
SAFETY LABEL
You can mark the pipette application your initials the calibration date, etc. on the safety
label.
Remove the clear plastic window on the edge closest to the push button (use the service
tool that comes with the pipette, or a screwdriver). Mark the adhesive label with a felt-
tipped or other pen and snap the window back in place.
SHELF HANGER
You can attach the pipette shelf hanger on a counter, pipette stand or anywhere where
you want to hang your pipette.
Clean the area where you plan to attach the shelf hanger. Apply two stickers to the
underside of the shelf hanger. Press the shelf hanger firmly into place -- on a shelf,
countertop or pipette stand. To use, hang the grippy finger rest on the shelf hanger.
PIPETTING TECHNIQUES
Push and release the push button slowly at all times,
particularly when working with high viscosity liquids.
Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone.
Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and
empty the tip 2-3 times with the solution that you will be pipetting. Hold the pipette in an
upright position while aspirating liquid. The grippy should rest on your index finger. Make
sure that the tips, pipette and solution are at the same temperature.
FORWARD TECHNIQUE
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm
and slowly release the push button. Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push button to the first stop. After a
delay of about one second, continue to depress the push button all the way to the
second stop. This action will empty the tip.
4. Release the push button to the ready position.
If necessary, change the tip and continue pipetting.
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Figures 4-7:
A = Ready position
B = First stop
C = Second stop
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REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very small
volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button.
This action will fill the tip. Withdraw the tip from the liquid touching it against the
edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
REPETITIVE TECHNIQUE
The repetitive technique offers a rapid and simple procedure for repeated delivery of the
same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button. This action will fill the tip. Withdraw the tip from
the liquid touching against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
PIPETTING OF HETEROGENEOUS SAMPLES
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the push button to the first stop,
making sure the tip is well below the surface.
2. Release the push button slowly to the ready position. This will fill the tip. Keep the
tip in the solution.
3. Depress the push button to the first stop and release slowly. Keep repeating this
procedure until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.
CALIBRATION
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water. The pipettes are constructed to permit re-adjustment for liquids
of different temperature and viscosity.
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DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation value of the balance should be
chosen according to the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.00 1 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are
done in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air
between 15°C to 30°C.
The relative humidity must be above 50%. Especially with volumes under 50 µl the air
humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
PROCEDURE TO CHECK CALIBRATION
The pipette is checked with the maximum volume (nominal volume) and with the
minimum volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is
done with both volumes. A pipette is always adjusted for delivery (Ex) of the selected
volume.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
TABLE 1: Maximum permissible errors according ISO8655
Range Channel Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1-10 µl 8, 12 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16
5-50 µl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0
30-300 µl 8, 12 300 ±8.0 ±2.7 3.0 1.0
30 ±8.0 ±26.7 3.0 10.0
ADJUSTMENT:
Adjustment is done with the service tool.
1. Place the service tool into the openings of the calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or counterclockwise to decrease the
volume.
3. After adjustment check the calibration according to the instructions above.
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FORMULAS FOR CALCULATING RESULTS
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense
water to the weighing vessel, note the reading and start a stopwatch. See how much the
reading decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be
10 seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an
evaporation trap or lid on the vessel is used the correction of evaporation is usually
unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and
pressure. A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table
on page 38.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a
pipette.
A = V - V0A = inaccuracy
V = mean volume
V0= nominal volume
Inaccuracy can be expressed as a relative value: A% = 100% x A / V0
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard
deviation (s) or coefficient of variation (cv)
s = standards deviation
v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV) CV = 100% x S / V
MAINTENANCE
When the Finnpipette Digital Multichannel is not in use, make sure it is stored in an upright
position. We recommend a Finnpipette stand for this purpose.
SHORT-TERM CHECKING
The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except 70 % ethanol
should be used to clean the pipette.
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LONG-TERM MAINTENANCE
If the pipette is used daily it should be checked every three months. The servicing
procedure starts with the disassembly of the pipette.
DISASSEMBLING ASSEMBLING MULTICHANNEL PIPETTES
1. Press down the tip ejector.
2. Insert the maintenance pliers under the tip ejector bar to release the tip ejector.
3. Remove the tip cone module by pressing it with the maintenance pliers
4. Press the spring and remove the locking pieces from the groove. Remove the
spring, spring support and o–ring.
5. Place the maintenance key in the adapter groove and pull off the adapter.
6. Pull out the tip ejector adapter. Lift the upper end of the tip ejector bar slightly and
push it back. Lift out the module spring.
7. Use a screwdriver to remove the four/six screws in the module cover and lift off
the cover.
8. Remove the piston bar and clean the pistons with a dry nap–free cloth.
9. Clean the tip cones.
10. If needed, replace the seal by carefully releasing the cover ring from its snap joint
with the screwdriver. Remove all the parts from the tip cone. Clean all the parts.
Reassemble the tip cone.
5–50 µl and 50–300 µl: Take one piston. Slide cover ring 32 (larger hole), spring 33,
support ring 35, (o–ring 37 bigger 5-50µl) and o–ring 36 (smaller) onto the piston.
Grease the o–ring with the lubricant provided in the pipette package. Slide all the parts
into the tip cone and close the snap joint of the cover ring.
0.5–10 µl: Take one piston. Slide cover ring 32 (larger hole), support 35, o–ring 36
(bigger), o–ring 37 (smaller) and o–ring support 38 onto the piston. Then slide
spring 39, spring support 40 (sharp edges first) and o–ring 41 onto the o–ring sup
port 38. Grease the o–rings with the lubricant provided in the pipette package. Slide
all the parts into the tip cone and close the snap joint of the cover ring.
11. Grease cleaned pistons with the lubricant provided in the pipette package.
12. Install the piston bar with pistons and tip cones in the cover and close the cover
with the four/six screws.
13. Place the tip ejector and module spring on the neck of the module. Press the spring
below the tip ejector. Close the tip ejector with the tip ejector adapter.
14. Use the maintenance key to slide the adapter to wider groove in the module neck.
15. Slide the o–ring, spring support and spring onto the piston bar and lock with the
locking pieces.
16. Attach the tip cone module to the handle and the tip ejector adapter to the tip ejector bar.
SERVICE INSTRUCTIONS FOR MULTICHANNEL PIPETTE TIP CONES
To ensure even performance between all channels in a multichannel pipette, all tip cones
have to be changed at the same time, if any of them needs to be changed. Don’t mix tip
cones of different packages, because one bag contains a matched set of tip cones.
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Solution
Attach firmly
Clean tip cones attach
new tips
Clean and grease O-ring
and cylinder.
Grease accordingly
Change the O-ring
Follow instructions carefully
Attach firmly
Recalibrate according
to instructions
Recalibrate with
the liquids in question.
Defect
Leakage
Inaccurate dispensing
Inaccurate dispensing
with certain liquids
TROUBLESHOOTING
The table below lists possible problems and their solutions.
Possible reason
Tip incorrectly attached
Foreign particles
between tip and tip cone
Foreign particles between the
piston, the O-ring and the cylinder
Insufficient amount of grease on
cylinder and O-ring
O-ring damaged
Incorrect operation
Tip incorrectly attached
Calibration altered: caused
by misuse, for example
Unsuitable calibration.
High viscosity liquids may
require recalibration.
STERILIZATION
The entire pipette can be sterilized by autoclaving it at 121°C (252°F) (minimum 20
minutes). No special preparations are needed for autoclaving. You can use steam
sterilization bags if needed.
After autoclaving the pipette must be cooled to room temperature for at least two hours.
Before pipetting, make sure that the pipette is dry. We recommend that you check the
calibration of after every 25th sterilization cycle.
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