Thermo Scientific STP-120 User manual

Thermo Scientific Spin Tissue Processor Microm STP 120
1
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Thermo Scientific
SPIN TISSUE PROCESSOR
Microm STP-120
INSTRUCTION MANUAL

Thermo Scientific Spin Tissue Processor Microm STP 120
2
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
SPIN TISSUE PROCESSOR
Model STP-120
INSTRUCTION MANUAL
Version 2.30
English
04/2011

Thermo Scientific Spin Tissue Processor Microm STP 120
3
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Safety Precautions..........................................................................
Warning symbols.........................................................................
Intended use and safety precautions...........................................
Certification.................................................................................
Warranty .....................................................................................
Technical characteristics.................................................................
Reception and emplacement ..........................................................
Transport and unpacking.............................................................
Emplacement..............................................................................
Connection..................................................................................
Control panel..................................................................................
Programming..................................................................................
Basket cycle................................................................................
Example of a process..................................................................
Entering the program...................................................................
Starting the program....................................................................
Delayed start...............................................................................
Stopping the program..................................................................
End of the program......................................................................
Temperature display....................................................................
End of process display................................................................
Setup Menu....................................................................................
Changing the language...............................................................
Changing the date and time ........................................................
Working with two baskets............................................................
Power failure...............................................................................
Emergency Movements...............................................................
Maintenance...................................................................................
Cleaning......................................................................................
Changing the filter.......................................................................
Replacing the battery ..................................................................
Alarms.........................................................................................
Reseting the unit.........................................................................
Reagents.....................................................................................
Technical Service Centres...........................................................
Contents

Thermo Scientific Spin Tissue Processor Microm STP 120
4
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Safety precautions
These instructions should be read carefully and fully
understood before installing and operating the unit.
This manual uses the following symbols:
DANGER:
Special warning messages to prevent harm to persons and/or
serious damage to equipment. For your own safety, please
observe these instructions carefully.
CAUTION, WARNING:
Special precautionary measures to prevent damage to equipment.
For a long lifetime of the equipment, please observe these
instructions carefully.
NOTE:
Special instructions regarding operation of the instrument.
BIOHAZARD:
Warning of biological danger.
Warning symbols $
&
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Thermo Scientific Spin Tissue Processor Microm STP 120
5
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Dear valued Customer,
Thank you for buying this instrument.
Before putting the instrument into operation, please read these
operating instructions carefully to familiarize yourself with its
proper operation and function. Non-compliance with these
instructions means that you are invalidating the standards of safety
and use for which this instrument was designed.
The STP 120 is an efficient spin tissue processor with carousel
system for embedding specimens in paraffin.
Only skilled or specially trained personnel must operate the
instrument. The listed and marked safety measures as well as the
regulations and hygiene measures of your respective lab must
strictly be observed.
Ser. No………………………………………..
Please enter the serical number, which is placed on the type plate
on the rear side of the instrument. This way, questions and service
can be handled faster.
Operating instruction no.: 387718
Issued on April 18th, 2011
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf
Germany
Phone: [phone]
Fax: [phone]
Email: in[email protected]
Internet: www.thermoscientific.com
Intended use and safety
precautions

Thermo Scientific Spin Tissue Processor Microm STP 120
6
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
DANGER:
INSTRUMENT GROUNDING
To avoid injury from electrical current, the instrument must be
connected with the safety ground. The instrument is equipped with
a three wire ground plug. The power outlet must be connected to
the safety ground and must meet the International Electrotechnical
Commission (IEC) regulations.
DANGER:
MAINS VOLTAGE
Do not remove the covers from the instrument during operation.
Any replacement or adjustment of components should only be
carried out by authorised trained maintenance staff. Disconnect
the unit from the mains power supply before opening or removing
any covers.
DANGER:
RISK IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable
gases.
DANGER:
RISK OF RADIOACTIVE RADIATION
When working with radioactive specimens, all procedures
applicable to protection against radiation should be observed.
DANGER:
RISK OF INFECTION
Use appropriate safety and disinfection measures when working
with infectious specimens.
DANGER:
HAZARD OF MALFUNCTION
To avoid the hazard of malfunction of an instrument, it must only
be operated in a controlled electromagnetic environment. This
means that transmitters such as mobile phones must not be
operated in their close vicinity.
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Thermo Scientific Spin Tissue Processor Microm STP 120
7
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
HAZARD OF BIOLOGICAL DANGER
Specimens used during the intended operation of the instrument
might potentially be infectious. For this reason, it is recommended
to observe the general laboratory regulations concerning
protection against danger of infection. Information on
decontamination media, their use, dilution and effective range of
application can be read in the Laboratory Biosafety Manual : 1984
of the World Health Organization.
When working with radioactive contaminated material, appropriate
safety and disinfection measures must be carried out. According to
the rules and regulations concerning the handling of radioactive
contaminated material of the respective laboratory, safety clothing
(e.g. particle mask, gloves, protective shoe covers) must be worn.
Separate taking back of electrical and electronic instruments
in the countries of the European Union:
This is to be applied in countries of the European Union and other
European countries with a separate collecting system within the
waste management.
This product, being an electro and/or electronic instrument, must
be treated separately within the waste management process
(WEEE).

Thermo Scientific Spin Tissue Processor Microm STP 120
8
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Thermo Fisher Scientific Microm International GmbH certifies that
this instrument has been tested and checked carefully. Its
technical data was verified before shipment to be in accordance
with the published specifications. The instrument complies with
applicable international safety regulations.
This Thermo Scientific product is warranted against defects in
material and workmanship for a period of 1 year. Parts which
prove to be defective during the warranty period will be repaired or
replaced free of charge by Thermo Fisher Scientific Microm
International GmbH. No other warranty is expressed or implied.
Unauthorized modification or repair by third party persons will void
the warranty.
The warranty will expire in case of improper or wrong use of the
instrument and in case the warning and precautionary messages
are not observed. Thermo Fisher Scientific Microm International
GmbH is not liable for any occurring damage.
Once the guarantee period has expired, a maintenance contract
should be signed to ensure the unit is kept in optimum operating
condition.
For more information about maintenance contracts, please contact
your local distributor.
Although every effort is made to ensure that the characteristics
and information contained in this manual are correct, undetected
printing errors are beyond the manufacturer's control and so no
responsibility can be accepted.
This manual is subject to modifications and changes without
previous notification.
This instruction manual will be supplied together with each
instrument. Further copies can be ordered at the nearest sales
office by giving the serial number of the instrument, the number of
the instruction manual and the date of issue.
Cat. no.
German 387717
English 387718
Spanish 387720
French 387719
Certification
Warranty

Thermo Scientific Spin Tissue Processor Microm STP 120
9
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Technical characteristics
Type STP-120
EC Directives Refer to notes on next pageApplicable Standards
Electromagnetic compatibility
SizesDiameter of the carrousel 850 mm
Height 500 –700 mm
Diameter of the turning circle 670 mm
Weight
Including packing 145 kg
Net (fully equipped) 70 kg
Electrical installation
Rated Voltage (±10%) 100…120 V 1.7 A 50…60 Hz
220…240 V 0.9 A 50…60 Hz
Fuses 115 V (2 x T4AH)
230 V (2 x T2AH)
Battery Nickel-Cadmium (12V 600
mA)
Overvoltage category 2
Pollution degree II
Sound pressure < 70 dBA
Capacity
Reagent stations
Number of reagent vessels 10 (9, if 3 are for paraffin.)
Volume per reagent vessel 1.8 L
Baskets for the specimen holders
Number of baskets 1 (option of 2)
Basket capacity 120 specimen holders
Paraffin stations
Number 2 (option of 3)
Volume 1.8 L
Rated Voltage 24 V ~
Rated power per station 100 VA
Temperature range 50 –70 °C
Hysteresis margin 0.1 –2 °C
Temperature cut-off 80 °C (± 4 °C)
Thermal protection Manual reset

Thermo Scientific Spin Tissue Processor Microm STP 120
10
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Programming
Number of programs 10 (selectable)
Programmable infiltration time per station from 1 s to 99 h 59 m
Rotation stirring selectable
Vertical shake selectable
Centrifugation selectable
Centrifugation time selectable
Program start delay selectable without time limit
Environmental conditions
at a max. rel. humidity of 60% without condensation
Temperatures from + 5 to + 40 °C
Storage temperature range from –20 °C to +50°C
Altitude up to 2000 m M.S.L.
Floor loading requirements 160,0 kg /m2
For indoor use only

Thermo Scientific Spin Tissue Processor Microm STP 120
11
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Reception and emplacement
During transport the carrousel should always be kept in an upright
position.
Your carrousel SPIN TISSUE PROCESSOR has been fully tested
and has undergone rigorous processes of verification and control
before being packed. Open the packing carefully and make sure
that the machine has not suffered any damage during transport. If
any damage is observed, immediately report this fact to the
distributor and the company insuring the transport.
Remove any support or brace holding the machine inside the
packing box.
CAUTION:
Two people are required to remove the carrousel from the packing
box. Take care to always hold the carrousel as shown in the figure.
Never lift the processor by the upper cover of the carrousel as
this will cause irreparable damage to the unit!
Transport and unpacking
&

Thermo Scientific Spin Tissue Processor Microm STP 120
12
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Choose a suitable flat surface measuring at least 900 mm in width
x 900 mm depth and with 750 mm of headroom.
CAUTION:
During operation, the cover of the processor is raised about 200
mm above its rest position.
It is absolutely necessary that the emplacement of the
processor has at least 750 mm of free headroom otherwise
the carrousel will be irreparably damaged when it opens.
Make sure that:
nthe surface is perfectly smooth, flat and stable.
nthe site is well ventilated.
nthere is sufficient surrounding space to allow easy access while
safely supporting the weight of the machine (Refer to sizes and
weights in the section on characteristics).
WARNING::
When selecting the emplacement for the machine, remember that
the Main ON/OFF switch is located at the rear and that it must be
accessible at all times.
Manual rotation of the whole unit:
Apart from the automatic rotating system for the processor cover,
the base of the unit is also fitted with wheels. This enables the
whole unit to be turned by hand to enable easy access to all the
reagent vessels and paraffin baths should it be placed close
against a wall.
CAUTION
Never turn the unit holding it by the cover.
Before turning the unit, hold it by the reagent vessel support table
and proceed as follows:
nuse the €button, to raise the carrousel cover to its uppermost
position.
nturn off the unit at the Main ON/OFF switch and unplug the
power supply cable (see next page). This will enable the unit to
be turned on its axis without any risk of damaging the power
cable.
Emplacement
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Thermo Scientific Spin Tissue Processor Microm STP 120
13
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
The electronic unit of the carrousel is suitably protected and
adequately shielded according to EC Standards on emission and
reception of interference. Nevertheless, the unit should not be
installed close to other high power equipment that could generate
vibrations or strong electrical interference.
The carrousel is supplied with a power cable for connection to a
standard outlet with earth. The electrical connection is located at
the rear.
CAUTION:
Before connecting the power cable, make sure that the machine is
adjusted to operate at the supply voltage.
The factory settings for the power supply are for operation with
220-240 V ~. If the unit is operated with 100 –120 V~, open the
cover of the power plug, remove the fuse holder and invert its
position so that the visor shows 115 V the correct operating
voltage. The fuses should also be replaced by others
corresponding to rated value as indicated in the table below.
1Insert a screwdriver here to open the cover of
the power plug and invert the fuse-holder with
the voltage selector
2Window showing the operating voltage (in the
example 220V)
3On/Off switch.
Pressing:
I = start
O = stop
(the image shows the stop position)
4Standard power plug with earth connection.
Values of the fuses
depending on the operating
voltage
110-120 V -4 A (2 x T4AH)
220-240 V -2 A (2 x T2AH)
Connection
&

Thermo Scientific Spin Tissue Processor Microm STP 120
14
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Initial turn-on
Before assembling the baths, start the unit to raise the carrousel.
DANGER:
Never attempt to turn, raise or lower the carrousel cover by
hand or force it in any way!
Apart from the risk of personal injury, the machine could
suffer irreparable damage. Always use the keys on the control
panel to make any manual movements and keep hands away
from any moving parts.
Connect the power supply cable and start the unit using the stop-
start switch located at the rear of the unit, above the plug for the
power supply cable.
When the power is connected the unit automatically begins a
machine test cycle and the fume exhaust fan will being operating.
This message appears on the display (V1.XX indicates the
software version).
This is followed by:
FILTER CHANGE XXXX HOURS (Refer to 'Changing the filter),
and then:
C10 C11 C12
16º 16º 16°
indicating the temperature of the paraffin baths.
NOTE:
These messages may appear in a language other than your own.
If you want to change the language now, refer to section Changing
the language.
Furthermore, the date and time perhaps do not coincide with those
in your country. If you want to adjust the time, refer to chapter
Changing the date and time.
The display indicates (in the pre-set language), a message like the
one shown here.
Press the €button once. Note that the carrousel opens to a half-
way position and the basket holder begins to turn.
When the carousel stops opening, press the €key once again to
continue opening the basket holder as far as possible. The screen
will display the message indicating 'Take sample' and the reagent
vessel located at 'C01'.
If the basket holder is not physically in the position of reagent
vessel 1, press the key •repeatedly to advance the basket to the
position of reagent vessel No 1. Disconnect the unit from the
power supply.
$
SPIN TISSUE
PROCESSOR V1.XX
•
01/01
READY 12:50
TAKE SAMPLE
C 011

Thermo Scientific Spin Tissue Processor Microm STP 120
15
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Now you can place the reagent vessels and paraffin baths in
position.
Connect the paraffin baths.
CAUTION
Before connecting or disconnecting the paraffin baths, make sure
that the unit is disconnected from the power supply by turning
off the main switch located at the rear of the unit.
Now you can turn the unit on again.
&

Thermo Scientific Spin Tissue Processor Microm STP 120
16
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Main parts of the spin tissue processor
The model shown in the figure corresponds to the version with two
basket holders, three paraffin baths and glass reagent vessels.
Depending on the processor model, the reagent vessels may be
either glass or aluminium:
The marks in the centre indicate the
maximum fill level for the reagent or
paraffin.
The dimple on the bottom allows the
vessel to be correctly centred in
position.
DANGER:
Under no circumstances should the maximum fill level be
exceeded as the process will not be performed correctly and
reagent or paraffin could be spilled.
!
$
Carrousel cover
Basket holder
Reagent vessels
Paraffin baths
Control Panel
Basket with specimen
s

Thermo Scientific Spin Tissue Processor Microm STP 120
17
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Connection of the paraffin baths
The paraffin baths are connected from the rear of the unit.
For easier access to the rear part of the apparatus, the whole unit
can be turned on itself.
Do not turn the unit holding it by the carrousel cover. Refer to
instructions and precautions to be taken when turning the unit by
hand.
CAUTION
Before connecting or disconnecting the paraffin baths, make sure
that the unit is disconnected from the power supply by turning
off the main switch located at the rear of the unit.
CAUTION:
Always use the following connections:
nthe connection marked with
the number 10 only if a third
paraffin bath has been
installed in position 10
nthe connection marked with
the number 11 only if the
paraffin bath has been
installed in position 11
nthe connection marked with
the number 12 only if the
paraffin bath has been
installed in position 12
Note the grooves in the edge of the processor table to allow
connection of the cables to the paraffin baths.
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Thermo Scientific Spin Tissue Processor Microm STP 120
18
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Placement of the baskets
The baskets are held in position
by a four point bayonet
connector.
When fitting the baskets, make
sure that the four studs fit into
the four grooves on the edge;
turn the basket as far as
possible inside the groove and
allow it to rest on the pivots of
the basket holder (see figure).
Before beginning to process tissues, you should become familiar
with the handling and programming of the machine. Design a small
test program and perform some manual movements without any
reagents in the vessels or samples in the baskets.

Thermo Scientific Spin Tissue Processor Microm STP 120
19
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
Control panel
The control panel at the front of the carrousel contains all the keys
required to handle and program the carrousel. The keyboard is of
the membrane type. Activate the keys by pressing in the centre of
the corresponding icon or indicator. Do not use sharp objects as
they could damage the membrane and the keyboard.
The actions of the keys:
SHAKE
Vertical shake. Pressing this key lights the
indicator light and activates the SHAKE function.
This function enables vertical shaking of there
basket during the stirring process (refer to
Basket cycle).
•
Press this key to make the machine perform a
partial rotation, moving the basket holder to the
next station. This is only possible when the
basket holder is in the upper position.
€
This key raises and lowers the basket holder as
required. If it is pressed when the basket holder
is moving, it will reverse the direction. The STOP
key stops all movement
POWER
The LED lights up to indicate that the unit is
connected to the power supply.
A flashing LED indicates that the unit is
operating on the emergency battery (for more
information refer to section Power failure)
START
Key to start the automatic operating cycle
(program). When the unit is operating in
automatic mode, the pilot beside the key is lit.
STOP
Key to stop the automatic operating cycle. If
this key is pressed the microprocessor asks for
confirmation of whether the process is to be
aborted. Once the stop is confirmed, the
program is cancelled and the machine must be
controlled in manual mode using the keys €
and •to place the basket holder above the
first reagent vessel. The STOP key also halts
any movement of the machine at any time.
TIMER
Allows delaying the start. Refer to Delayed
start. When the TIMER is active the display
appears as dimmed the pilot light of the key is
lit
LOCK
Pressing this key blocks all the keys on the
control panel (to prevent accidental operation).
Restore the key actions by holding down this
key for more than two seconds.
x2
This key should always be pressed when the
machine is fitted with a double basket holder
(optional accessory). For more information,
refer to section Working with two baskets
UP
When adjusting values it
increases the value
displayed, or goes back to
the previous option
DOWN
When adjusting values it
decreases the value
displayed, or goes to the
next option.
PROGRAM
Use this key to access the
mode for editing programs
or making adjustments.
ENTER
Validates the option or
adjustments made.
For more information, refer
to Programming
08/02
READY
09:12

Thermo Scientific Spin Tissue Processor Microm STP 120
20
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 718 -English
CAUTION
Before executing the first real process with reagents and paraffin,
remember that paraffin can sometimes take a long time to melt.
After a long period of disconnection (for example the first start or
after power failure), the basket will not descend into the paraffin
bath (even though this is programmed) until the default safety
period has elapsed.
When testing the programming, skip paraffin bath positions 11 and
12 (and 10 if working with two baskets) by assigning them a time
period of 00:00.
If you program a time period of more than 00:00 in positions 11
and 12 (10, if working with three paraffin baths), the screen will
display this message, where XX is the number of the reagent
vessel programmed prior to paraffin bath 11 (10 if a third bath is
installed).
•
WAITING FOR PARAFFIN
Po 1•• C XX••• TR=00:00