Thermo Scientific thermoscientific Vanquish UHPLC System User manual

thermoscientific
Vanquish
UHPLC System
Operating Manual
4820.3601-EN Revision 4.0 •December 2019

Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved.
Original Operating Manual
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Contacting Us
UHPLC System
Operating Manual
Page 3

Contacting Us
Page 4 UHPLC System
Operating Manual

Contents
UHPLC System
Operating Manual
Page 5
Contents
1 Using this Manual ........................................................................ 9
1.1 About this Manual ................................................................................................10
1.2 Conventions..........................................................................................................11
1.2.1 Safety Messages....................................................................................... 11
1.2.2 Special Notices and Informational Notes ................................................. 11
1.2.3 Typographical Conventions ...................................................................... 12
1.3 Reference Documentation ...................................................................................13
2 Safety......................................................................................... 15
2.1 Safety Symbols and Signal Words.........................................................................16
2.1.1 Safety Symbols and Signal Words in This Manual .................................... 16
2.1.2 Observing this Manual ............................................................................. 16
2.1.3 Safety Symbols on the System ................................................................. 17
2.2 Intended Use ........................................................................................................18
2.3 Safety Precautions................................................................................................19
2.3.1 General Safety Information...................................................................... 19
2.3.2 Qualification of the Personnel ................................................................. 19
2.3.3 Personal Protective Equipment................................................................ 21
2.3.4 General Residual Hazards......................................................................... 22
2.3.5 In Case of Emergency ............................................................................... 24
2.4 Solvent and Additive Information ........................................................................25
2.4.1 General Compatibility .............................................................................. 25
2.4.2 Allowed pH Ranges................................................................................... 26
2.4.3 Allowed Concentrations ........................................................................... 26
2.4.4 Further Information ................................................................................. 27
3 System Overview ....................................................................... 29
3.1 System Description (Standard Configuration) ......................................................30
3.1.1 Solvent Rack ............................................................................................. 31
3.1.2 System Base ............................................................................................. 32
3.2 Operation .............................................................................................................33
4 Unpacking .................................................................................. 35
4.1 Unpacking.............................................................................................................36
4.2 Scope of Delivery..................................................................................................37

Contents
Page 6 UHPLC System
Operating Manual
5 Installation................................................................................. 39
5.1 Safety Guidelines for Installation .........................................................................40
5.2 Installing the System ............................................................................................41
5.3 Site Requirements ................................................................................................43
5.3.1 Workbench............................................................................................... 43
5.3.2 Power Considerations .............................................................................. 44
5.3.3 Power Cord............................................................................................... 44
5.3.4 Condensation ........................................................................................... 45
5.3.5 Operating Conditions ............................................................................... 45
5.4 Setting Up the Hardware......................................................................................46
5.4.1 System Stack Setup .................................................................................. 46
5.4.2 Signal Cable Connections ......................................................................... 54
5.4.3 Power Connections .................................................................................. 59
5.4.4 Cable Clips ................................................................................................ 60
5.4.5 System Base Lock ..................................................................................... 61
5.5 Setting Up the Flow Connections .........................................................................64
5.5.1 General Information and Guidelines........................................................ 64
5.5.2 Flow Connections Overview..................................................................... 65
5.5.3 Guiding Capillaries and Tubing Through the System................................ 66
5.5.4 Connecting Fittings, Capillaries, and Tubing ............................................ 68
5.5.5 Connecting the Waste Lines..................................................................... 70
5.6 Turning On the System .........................................................................................78
5.7 Setting Up the System in the Software.................................................................79
5.8 Flushing the System..............................................................................................81
6 Operation................................................................................... 83
6.1 Introduction to this Chapter.................................................................................84
6.2 Safety Guidelines for Operation ...........................................................................85
6.3 Power On/Off Control ..........................................................................................86
6.4 Preparing the System for Operation.....................................................................87
6.5 Use of Solvents and Additives ..............................................................................89
6.6 Operating the System from the Software ............................................................92
6.6.1 Starting the Instrument Controller and Client ......................................... 92
6.6.2 Controlling the System from the ePanel Set ............................................ 93
6.6.3 Monitoring the Baseline........................................................................... 94
6.6.4 Running Automated Sample Analysis....................................................... 95
6.7 Shutting Down the System ...................................................................................97

Contents
UHPLC System
Operating Manual
Page 7
7 Maintenance and Service ......................................................... 101
7.1 Introduction to Maintenance and Service......................................................... 102
7.2 Safety Guidelines for Maintenance and Service................................................ 103
7.3 General Rules for Maintenance and Service ..................................................... 105
7.4 Routine and Preventive Maintenance............................................................... 106
7.4.1 Maintenance Plan .................................................................................. 106
7.4.2 Cleaning or Decontaminating the System .............................................. 106
7.5 Transporting or Shipping the System ................................................................ 109
8 Troubleshooting....................................................................... 113
8.1 General Information about Troubleshooting .................................................... 114
8.2 System Troubleshooting.................................................................................... 115
8.2.1 Peak Shape ............................................................................................. 115
8.2.2 Ghost Peaks, Negative Peaks and Spikes ............................................... 116
8.2.3 Peak Area Precision................................................................................ 117
8.2.4 Flow........................................................................................................ 119
8.2.5 Pressure.................................................................................................. 120
8.2.6 Retention Time Variation ....................................................................... 121
8.2.7 Baseline .................................................................................................. 121
8.2.8 Temperature Control ............................................................................. 123
8.2.9 Power and Communication.................................................................... 124
8.2.10 System Diagnostics................................................................................. 125
9 Specifications ........................................................................... 129
9.1 System Specifications ........................................................................................ 130
9.2 Solvent Rack and System Base Specifications ................................................... 131
10 Accessories, Consumables and Replacement Parts ................... 133
10.1 General Information.......................................................................................... 134
10.2 Ship Kit............................................................................................................... 135
10.3 Optional Accessories ......................................................................................... 137
10.4 Consumables and Replacement Parts ............................................................... 138
10.4.1 Capillaries and Tubing ............................................................................ 138
10.4.2 Solvents and Wash Systems ................................................................... 139
10.4.3 Fuses and Cables .................................................................................... 140
10.4.4 Other Parts ............................................................................................. 140

Contents
Page 8 UHPLC System
Operating Manual
11 Appendix.................................................................................. 141
11.1 Compliance Information.................................................................................... 142
11.1.1 Declarations of Conformity .................................................................... 142
11.1.2 WEEE Compliance .................................................................................. 143
11.1.3 FCC Compliance...................................................................................... 143
11.1.4 Manual Release History.......................................................................... 143
Index........................................................................................ 145

1 • Using this Manual
UHPLC System
Operating Manual
Page 9
1 Using this Manual
This chapter provides information about this manual, the conventions
used throughout the manual, and the reference documentation that is
available in addition to this manual.

1 • Using this Manual
Page 10 UHPLC System
Operating Manual
1.1 About this Manual
This manual provides instructions for installation, set up, start up, shut
down, operation, maintenance and troubleshooting of your system.
The layout of this manual is designed to provide quick reference to the
sections of interest to the user. To obtain a full understanding of your
system, read this manual thoroughly.
This manual also contains safety messages, precautionary statements,
and special notices that can prevent personal injury, damage to the
system, or loss of data when followed properly.
Note the following:
•The descriptions in this manual refer to a standard one-stack
VanquishTM system configuration. Not all descriptions necessarily
apply to your particular system.
•Illustrations in this manual are provided for basic understanding.
They can vary from the actual model of the system. However, this
does not influence the descriptions. No claims can be derived from
the illustrations in this manual.