Thermo Scientific ERGOSTAR HM 200 User manual

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
1
Thermo Scientific
SECTION TRANSFER SYSTEM Microm STS
For ROTARY MICROTOMES / ERGOSTAR HM 200
387712 Issue 8
INSTRUCTION MANUAL

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
2
CERTIFICATION
Thermo Fisher Scientific Microm International GmbH certifies that this
instrument has been tested and checked carefully. Its technical data was
verified before shipment to be in accordance with the published
specifications.
The instrument complies with applicable international safety regulations.
WARRANTY
This product is warranted against defects in material and workmanship for a
period of 1 year. Parts which prove to be defective during the warranty
period will be repaired or replaced free of charge by Thermo Fisher Scientific
Microm International GmbH. No other warranty is expressed or implied.
Unauthorized modification or repair by third party persons will void the
warranty.
The warranty will expire in case of improper or wrong use of the instrument
and in case the warning and precautionary messages are not observed.
Thermo Fisher Scientific Microm International GmbH is not liable for any
occurring damage.
Errors and omissions excepted. Subject to amendment and improvement
without further notice.
This instruction manual will be supplied together with each instrument.
Further copies can be ordered at the nearest sales office by giving the serial
number of the instrument, the number of the instruction manual and the date
of issue.
This instruction manual is available in the following languages:
Cat. No.
German: 387711
English: 387712
French: 387713

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
3
INTENDED USE
Dear Customer,
Thank you for buying this Thermo Scientific instrument.
Before putting the instrument into operation, please read these
operating instructions carefully to familiarize yourself with its proper
operation and functions.
Only skilled or specially trained personnel must operate the
instrument, i.e. clamping the specimen, trimming, sectioning and
taking off the sections from the instrument. The listed and marked
safety measures as well as the regulations and hygiene measures of
your respective lab must strictly be observed.
Ser. No. :..................................................
Please enter the serial number, which is placed on the type plate on
the rear side of the instrument. This way, questions and service can
be handled faster.
Instruction manual no. 387712
Issued on 02.10.2014

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
4
Intended use
Safety precautions
PART 1 INTRODUCTION
1-1 Description of the Section Transfer System STS
1-2 Technical data Section Transfer System
PART 2 OPERATING INSTRUCTIONS
2-1 Initial turn-on
2-2 Trim sectioning of specimens
2-3 Fine sectioning of specimens
2-4 Draining and cleaning the water bath and the pump reservoir
2-5 Cleaning the pump filter
2-6 Disposable blade carrier TE
2-7 Standard equipment
2-8 Additional equipment
PART 3 THEORY OF OPERATION OF THE SECTION TRANSFER SYSTEM
3-1 Water circulation
3-2 Cutting and detaching a section
PART 4 WORKING WITH THE SECTION TRANSFER SYSTEM
4-1 Preparing the Section Transfer System
4-2 Coarse feed and trimming function
4-3 Sectioning and picking up sections
4-4 Working with the STS in combination with the Cool-Cut
4-5 How to avoid malfunctions
PART 5 MAINTENANCE
PART 6 CLEANING AND CARE
6-1 Cleaning
6-2 Disposal of the instrument after final shutdown

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
5
SAFETY PRECAUTIONS
WARNING SIGNALS AND SYMBOLS
The installation and routine use of the STS is easy and safe if the instructions in this manual are being
observed.
However, the situations which might be risky for the personnel or equipment are specially marked in
this manual with the following symbols and messages.
Note:
Special instructions regarding operation of the instrument.
Caution:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.
Danger:
Special warning messages to prevent harm to persons and/or serious damage to equipment.
For your own safety, please observe these instructions carefully.
Biohazard:
Warning of biological danger.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European countries
with a separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately
within the waste management process (WEEE).

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
6
SAFETY PRECAUTIONS
A T T E N T I O N !
Please observe the following general precautions during operation of this instrument.
Failure to comply with these precautions violates safety standards and the intended
use of the instrument. Thermo Fisher Scientific Microm International GmbH is not
liable for misuse of the instruments and failure to comply with basic safety
requirements.
INSTRUMENT GROUNDING
To avoid injury from electrical current, the instrument must be connected with the safety ground. The
instrument is equipped with a three wire ground plug. The power outlet must be connected to the
safety ground and must meet the International Electro technical Commission (IEC) regulations.
CAUTION: MAINS VOLTAGE
Never remove instrument covers during operation. Component replacements as well as adjustments
must only be made by trained service personnel. Unplug the unit before removing or opening the
covers. Only original spare parts must be used for replacement work.
DANGER IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable gases.
HAZARD OF RADIOACTIVE RADIATION
When working with radioactive specimens observe all applicable radiation safety procedures. When
working with radioactive contaminated material, appropriate safety and disinfection measures must be
carried out. According to the rules and regulations concerning the handling of radioactive
contaminated material of the respective laboratory, safety clothing (e.g. particle mask, gloves,
protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
7
HAZARD OF WATER
Set up and operate the separate control unit in a way that it is protected from water.
HAZARD OF BIOLOGICAL DANGER
Specimens used during the intended operation of the instrument might potentially be infectious. For
this reason, it is recommended to observe the general laboratory regulations concerning protection
against danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be
read in the Laboratory Biosafety Manual : 1984 of the World Health Organization.
HAZARD OF MALFUNCTION
To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled
electromagnetic environment. This means that transmitters such as mobile phones must not be
operated in their close vicinity.
DANGER WHEN USING OPERATING MATERIALS
Xylene and other solvents may be considered hazardous. For this reason, please observe the
stipulated safety measures to avoid contact with eyes and skin (e.g. wear protective gloves).
WASTE DISPOSAL
All debris, waste as well as infectious and radioactive contaminated material from operation must be
disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning liquids
as well as section waste must be disposed of according to the respective regulations for special waste!

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
8
Fig. 1
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Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
9
1 INTRODUCTION
1-1 Description of the Section Transfer System STS
The system consists of a special disposable blade carrier TE with integrated transfer bridge, a heated
water bath as well as a control unit.
The Section Transfer System can be used along with all current rotary microtomes and the Ergostar
HM 200.
It takes a few minutes only to upgrade the microtome from the common paraffin sectioning technique
to the STS method.
The STS is used solely for tissue specimens embedded in paraffin. During the sectioning process with
the STS, sections glide on a water film via the disposable blade carrier TE and the transfer bridge and
are then transferred into the water bath.
Water circulation is guaranteed by a pump, the capacity of which can be adjusted.
The water temperature of the water bath can be adjusted between ambient temperature and +45°C.
Only use demineralized or distilled water!!
Due to the use of the STS damage to the tissue sections can be diminished by gliding on the water
flow. The sections are stretched during the cutting process due to the smooth water flow. This way,
extremely thin as well as high-quality sectioning results can be obtained.
The common manual transfer of the sections into a water bath is no longer necessary.
When the sections are generated, their quality can be checked immediately, i.e. when the ribbon or
the individual sections are stretched on the water flow of the disposable blade carrier TE. The second
quality check can be carried out in the water bath facilitated by the integrated section illumination.
The sections which are not used, e.g. from trimming paraffin blocks, can be guided directly into a
waste basket via the water flow on the disposable blade carrier TE.
The water bath, the waste container as well as the waste filter of the STS can easily be removed and
cleaned.
The integrated water container for the pump circulation is freely accessible and allows for easy
cleaning.

Thermo Scientific Section Transfer System Microm STS
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
10
1-2 TECHNICAL DATA SECTION TRANSFER SYSTEM
Heated water bath: +45°C
Volume of heated water bath: 800 ml
Volume of pump reservoir: 600 ml
Required liquid: demineralized or distilled water
Flow rate: 0 ml/min. - 500 ml/min.
Operating conditions: +10°C to + 35°C (at max. 60% rel. humidity of the air)
altitude up to 2000 M.S.L.
for indoor use only
Pollution degree: 2
Overvoltage category: II
Acoustic pressure: 40 dB(A)
Power requirements: 220 - 240 V, 50/60 Hz, 0,7 A
100 - 120 V, 50/60 Hz, 1,4 A
Internal protection of the secondary circuit: 1 x T4AH; 1 x T2AH
Weight: control unit: 2,4 kg
STS complete: 4,4 kg
disposable blade carrier TE: 2,8 kg
Dimensions of the STS: Ø 300 mm x height: 130 mm
Dimensions of the control unit: 265 x 160 x 80 mm (long x wide x high)