Trace2O HydroTest HTTURB User manual

HydroTest
HTTURB
Portable Turbidity
Meter
Instruction Manual

Introduction
Thank you for selecting the HTTURB portable turbidity meter. This manual provides a step-by-step guide to help you operate the
meter. Please carefully read the following instructions before use.
UNPACKING THE METER
Before unpacking, ensure that the current work environment meets the following conditions.
Relative humidity is less than 80%.
Ambient temperature is greater than 0oC and less than 50oC.
No potential ambient light interference or electromagnetic interference.
The following list describes the standard accessories for the meter. After unpacking, please check all accessories are complete.
If any are damaged or missing, please contact your nearest distributor.
Operating Principles
The HTTURB portable turbidity meter operates on the nephelometric principle of turbidity measurement and is designed to meet
the criteria specified in ISO 7027. The following figure shows the basic optical system of the meter. It includes a light source and
a detector to monitor the light scattered at 90° with respect to the incident beam.

Overview
The HTTURB portable turbidity meter measures turbidity from 0 to 1100 NTU/FNU, or 0 to 275 EBC, or 0 to 9999
ASBC.
Seven optional parameters can be chosen in the setup menu.
Data can be stored and transferred to a computer by the USB interface.
Display
INDEX:
Low battery alarm
When the battery is depleted, this icon will
disappear.
Setup mode icon
Indicates the meter is in setup mode.
Measure mode icon
Indicates the meter is in measure mode.
Memory mode icon
Indicates data is stored into memory.
Calibration mode icon
Indicates the meter is in calibration mode.
Calibration icon
Indicates the meter is calibrating.
Sample chamber
USB interface
Alignment
arrow
Measurement
module
Battery compartment

Keypad
INDEX:
KEY
FUNCTION
MEAS|
Switches the meter on or off.
When in Measurement mode, press this key to take a measurement.
When in Calibration mode or Settings, press this key to exit to the Measurement mode.
CAL |
Press this key to enter Calibration mode.
Press and hold this key for 3 seconds to enter the Setup menu.
MI |◄
Press this key to store the current measured value to memory.
Press this key to increase the value or scroll up through the menu.
MR |►
Press this key to view stored data.
Press this ►key to decrease the value or scroll down through the menu.
ENTER │
Confirms the calibration setting, parameter value, or displayed option.

Inserting the Battery
The HTTURB portable turbidity meter is packaged with one 9V battery. Before using the device insert the battery into the battery
compartment following the procedure outlined below.
1. Remove the battery compartment cover from the meter’s case.
2. Insert the 9V battery into the battery compartment. Note the polarity of the terminals.
3. Replace the battery compartment cover to its original position.
4. Installation is complete.
When the battery is depleted, the meter allows you to use a USB cable connected to a computer as a temporary power
supply.
Power On/Off
●Press the key to turn on the meter. The display will show measuring values and mode icons.
●Press and hold the key for 3 seconds to turn off the meter.

Setup Menu
The HTTURB portable turbidity meter contains an integrated setup menu that allows you to customise display information, such
as date and time, as well as measurement settings, such as measurement resolution and turbidity units.
PARAMETER
DESCRIPTION
OPTIONS
DESCRIPTION
DEFAULT
Set the number of calibration
points
2 to 5 points
2
Date and Time
---
Sets the date and time for data log
---
Sets the default turbidity unit
NTU
Nephelometric Turbidity Unit
NTU
FNU
Formazin Nephelometric Unit
EBC
Turbidity scale of the European Brewery
Commission
ASBC
Turbidity scale of the American Society of
Brewing Chemists
Sets the resolution for
measurement
Resolution: 0.1
0.01
Resolution: 0.01
Set the measurement mode
Single measurement
YES
Continuous measurement
Auto-power off
Enable
NO
Disable
Clear all stored data
Enable
NO
Disable
Restore factory settings
Enable
NO
Disable
SETTING THE DEFAULT OPTION
1.1 Press and hold the key for 3 seconds to enter the setup menu.
1.2 Press the ◄or ►key to select a menu item, press the enter key to confirm
1.3 Press the ◄or ►key to select a desired option, press the enter key to confirm and return to measurement mode.

SETTING THE DATE AND TIME
1.1 Press and hold the key for 3 seconds to enter the setup menu.
1.2 Press the ►key once, the display will show DATE/P-2.
1.3 Press the enter key to confirm. The meter will show the year and the value will flash.
1.4 Press the ◄or ►key to set the year and the enter key to confirm.
1.5 Repeat step 2.4 to set the month, day and time. When the setting is complete, the meter will return to the measurement
mode automatically.
SETTING THE TURBIDITY UNIT
The HTTURB portable turbidity meter is capable of measuring results with one of four turbidity units, including the NTU, FNU,
EBC, and ASBC. The default setting is NTU. The following steps describe how to change the turbidity units of the instrument.
2.1 Press and hold the key for 3 seconds to enter the setup menu.
2.2 Press the ►key until the display shows UNIT/P-3.
2.3 Press the enter key to confirm. The meter will show the default turbidity unit.
2.4 Press the ◄or ►key to select the desired measurement unit.
2.5 Press the enter key to confirm and the meter will return to the measurement mode automatically.
SETTING THE RESOLUTION
The meter contains two resolution options. For high-accuracy measurements we recommend that you select 0.01. The default
option is 0.01. The following steps describe how to change the measuring resolution of the instrument.
3.1 Press and hold the key for 3 seconds to enter the setup menu.
3.2 Press the ►key until the display shows RESO/P-4.
3.3 Press the enter key to confirm. The meter will show 0.01/rESO

3.4 Press the ◄or ►key to select the desired resolution (0.1 or 0.01 NTU).
3.5 Press the enter key to confirm and the meter will return to measurement mode automatically.
CLEAR ALL STORED DATA
Please refer to page 17 (Clear Stored Data).
AUTO-POWER OFF
The HTTURB portable turbidity meter has an auto-power off function. When the auto-power off function is enabled the meter
will automatically turn off if you do not press any key within 15 minutes. The following steps describe how to set the auto-
power off function of the instrument.
4.1 Press and hold the key for 3 seconds to enter the setup menu.
4.2 Press the ◄or ►key until the display shows OFF/P-6.
4.3 Press the enter key to confirm. The meter will now show NO/OFF indicating that the auto-power off function is disabled.
4.4 Press the ◄or ►key to enable or disable this function.
4.5 Press the enter key to confirm and the meter will return to measurement mode automatically.
RESTORE FACTORY SETTINGS
The factory reset option is used to restore the meter back to default settings. If enabled, all of the calibration data and selected
options will be lost or reset; the meter must be recalibrated. If during calibration, the meter shows ERR and it cannot be corrected
by recalibration, the reset function will need to be enabled.
5.1 Press and hold the key for 3 seconds to enter the setup menu
5.2 Press the ◄or ►key until the display shows RST/P-8.
5.3 Press the enter key to confirm. The meter will show NO/rST which indicates the meter will not be reset.
5.4 Press the ◄or ►key to enable or disable this function.



5.5 Press the enter key to confirm and the meter will return to measurement mode automatically. The instrument is now
restored to factory settings.
EXIT THE SETTINGS
To exit the setup menu without saving changes, press the key.
SELECTING THE MEASUREMENT MODE
The HTTURB portable turbidity meter contains two measurement modes, single measurement and continuous measurement
mode. In the single measurement mode, the meter will automatically recognize and record a stable reading and lock the
measurement. In the continuous measurement mode, the meter will continuously update the measured values and can be used
to index or match sample vials. For more information, please refer to Addendum 1 (Indexing and Matching Sample Vials).
6.1 Press and hold the key for 3 seconds to enter the setup menu.
6.2 Press the ◄or ►key until the display shows HOLd/P-5.
6.3 Press the enter key to confirm. The display shows YES/HOLD indicating that the single measurement mode is selected.
6.4 Press the ►key, the display shows NO/HOLD indicating that the continuous measurement mode is selected.
6.5 Select one of the above options, press the enter key to confirm.
Important Notes for Measurement and Calibration
Do not hold the meter by hand during measurements and calibration.
Ensure that the sample in the vial is homogeneous and without air bubbles.
Before starting the measurement, the outside of the vial must be clean and dry. Wipe the vial with a lint-free cloth to
remove fingerprints and water marks.
If the vial has minor scratches or scuffs, add a few drops of silicone oil to the outside of the vial. Wipe with a lint-free
cloth to remove excess oil.
Glass vials and caps must be cleaned thoroughly with deionised water after each measurement. Minor residues from
previous samples can cause inaccurate readings.
Avoid spillage of water in the sample chamber as this may cause damage to the meter.
To prevent errors from ambient light interference, do not use the meter in direct or bright sunlight. Always close the
light shield lid during measurements and calibration.
Ensure that the vial is positioned in the sample chamber correctly, the mark on the vial must be aligned with the arrow
on the meter.


Turbidity Calibration
The HTTURB portable turbidity meter is calibrated with Formazin Standards at the factory and does not require user
calibration before use.
Do not shake or agitate the calibration standard violently; this is to prevent bubble formation.
In order to get accurate results we recommend that you calibrate the meter at least once a month.
The meter supports turbidity calibration up to 5 points with minimum of 2 points. The default calibration points include
0.02, 10.00, 200, 500, and 1000 NTU. During the calibration process you are able to modify the calibration point or
calibration values by pressing the ◄or ►key. For better accuracy, ensure to select a calibration point close to the
sample you are measuring.
SETTING THE NUMBER OF CALIBRATION POINTS
1.1 Press and hold the key for 3 seconds to enter the setup menu. The display will show CAL/P-1.
1.2 Press the enter key to confirm. The display will show 2/CAL.
1.3 Press the ◄or ►key to select the number of calibration points. Press the enter key to confirm. The meter will return to
measurement mode automatically.
CALIBRATE THE METER
2.1 Press the key to enter the calibration mode. The display will show 0.02NTU/CAL 1. If necessary, press the ◄or ►key
to select a calibration point close to the sample value you are measuring.
2.2 Take out the corresponding calibration standard from the carrying case. Wipe the vial with a lint-free cloth to remove
fingerprints.
2.3 Hold the vial cap and gently invert several times. Ensure that the calibration standard is homogeneous and has no air bubbles.
Alignment arrow
(meter)
Alignment arrow (vial)

2.4 Insert the calibration standard into the measurement chamber, ensuring the mark on the vial cap aligns with the arrow on the
meter. Close the light shield lid.
2.5 Press the enter key, the meter will begin calibration. The calibration icon will flash continuously.
2.6 Wait for the reading to stabilise, the meter will automatically show the next calibration point.
2.7 If necessary, press the ►key to select an applicable calibration point.
2.8 Repeat steps 2.2-2.5 until the meter shows End. Calibration is complete.
To exit the calibration mode without saving the calibration, press the key.
Turbidity Measurement
An accurate turbidity measurement depends on good measurement techniques. For example, ensuring the sample vials are
clean, aligning the vials in the sample chamber correctly, covering the vial with the light shield lid during measurements, regular
calibration of the meter, ensuring no bubbles are in the sample, etc. are all ways which will provide reliable results. For more
information, please refer to Important Notes for Measurement and Calibration.
SINGLE MEASUREMENT - SAMPLES WITH LOW TURBIDITY
For low turbidity samples (<200 NTU), we recommend that you use the same vial to perform the measurement and calibration.
3.1 Rinse the vial with approximately 10ml of the sample. Cap the vial with the screw cap and gently inverting it several times.
Discard the used sample and repeat the rinsing procedure twice more.
3.2 Fill the vial with the sample. Cap the vial.
3.3 Allow the vial to stand undisturbed for 1 minute so that bubbles can disappear.
3.4 Wipe the vial with the lint-free cloth to remove water drops and fingerprints. Ensure that the outside of the vial is dry and
clean.
3.5 Place the vial in the measurement chamber, ensuring the mark on the vial and the arrow on the meter are aligned.
3.6 Close the light shield lid. Press the key and the measure icon will begin flashing.
3.7 Wait for the reading to stabilise, the measure icon will stop flashing.

Press the key; the meter will take another measurement.
SINGLE MEASUREMENT - SAMPLES WITH HIGH TURBIDITY
For the high turbidity samples, the solution must be diluted prior to measurement. The dilution water can be obtained by filtering
deionised water through ha < 0.45 µm filter membrane.
4.1 Repeat steps 3.1 –3.5 above and record the measured value.
4.2 Calculate the true turbidity of the original sample using the following formula.
   󰇛󰇜

Where: T = true turbidity of the original sample
Td= measured value
Vs= volume of the original sample (ml)
Vd= volume of the dilution water (ml)
CONTINUOUS MEASUREMENTS
5.1 Ensure that the meter is in the continuous measurement mode.
5.2 Fill the vial with the sample. Cap the vial.
5.3 Gently invert the vial several times to mix the sample.
5.4 Wipe the vial with the lint-free cloth to remove water drops and fingerprints.
5.5 Place the vial in the measurement chamber. Ensure the mark on the vial and the arrow on the meter are aligned.
5.6 Close the light shield lid. Press the key. The measurement icon will begin flashing, the meter will continuously measure
the sample.
5.7 To stop the measurement, press the key again.

Storing and Recalling Data from Memory
The HTTURB portable turbidity meter is capable of storing and recalling up to 100 data sets.
STORING A MEASUREMENT RESULT
During the measurement process, press the MI key to input the measured value into the memory. The memory icon will appear
on the display.
VIEWING THE DATA LOG
1. Press the MR key in the Measure mode. The meter will show the latest stored data. E.g., LOC/1 (Data Log/1).
2. Press the ►key, the display shows the date and time of measurement (format mm-dd, hh-mm).
3. Press the ►key, the display shows the stored data.
4. Press the ►key, the display shows the next data set.
5. Press the key, the meter returns to the measurement mode.
If the meter has no stored data in the memory and the MR key is press, the meter will show - - - -.
Deleting the data log
When the meter memory is full, the display will show FULL when the MI key is pressed. To delete data logs, please follow the
steps below.
1. Press and hold the key for 3 seconds to enter the setup menu.
2. Press the ◄or ►key until the display shows CLR/P-7.
3. Press the enter key, the display shows NO/CLr.
4. Press the ►key to select YES/CLr.
5. Press the enter key to confirm.

Communication
The HTTURB portable turbidity meter is capable of transferring the data to a computer or importing the data to Excel by a Data
Acquisition System software. Before using the software ensure that the Windows 7/8/10 operating system has been installed on
your computer.
RECEIVING DATA
Connect the meter to the computer by a USB cable.
Click the DAS icon on the computer. The system will automatically scan for an available communication port and
show the message box “Found a port on your computer”.
Click the OK button to start the application. If the computer cannot find a communication port, please click the file
“CP210xVCPInstaller_x64.exe”or “CP210xVCPInstaller_x86.exe”to update the drive programme.
Click the “Connect” button. The screen will show “Port is connected” indicating that communication between the meter
and the computer has been established.
Click the OK button to confirm.
Click the Receive button to send any stored data in the meter to the computer.
CREATE AN EXCEL FILE
When the transfer is complete click the “Save as Excel” button. The measured values in the data sheet will
automatically convert to an Excel file.
WARNING:
Once the software is closed, all received data will be lost and cannot be recovered.

Specifications
Model
HTTURB
Measurement Method
ISO 7027 nephelometric method (90°)
Measurement Range
0~1100 NTU/FNU, 0~275 EBC, 0~9999 ASBC
Resolution
0.01 (0~100 NTU), 0.1 (100~999 NTU), 1 (999~1100 NTU)
Accuracy
±2% (0~500 NTU), ±3% (501~1100 NTU)
Calibration Standards
0.02 NTU, 10.00 NTU, 200 NTU, 500 NTU, 1000 NTU
Light Source
Infrared-emitting diode
Detector
Silicon Photodiode
Stray Light
<0.02 NTU
Sample Vials
60(H) ×25(Dia)mm
Memory
Stores up to 100 data sets
Output
USB Communication Interface
Operating Temperature
0~50°C, 32~122°F
Power Requirements
1×9V Battery
Dimensions
180(L)×85(W)×70(H)mm
Weight
300g
Addendum 1. Indexing and Matching Sample Vials
The United States Environmental Protection Agency (U.S. EPA) recommends that vials used for turbidity meter calibration or
sample measurement be indexed.
Its purpose is to obtain a position which provides the lowest turbidity reading. The indexing methods are as follows.
1. Fill the vial using distilled water (<0.5 NTU). Cap the vial.
2. Wipe the vial with a lint-free cloth to remove water marks and fingerprints.
3. Let the vial stand undisturbed for 5 minutes.
4. Place the vial in the sample chamber aligning the arrow on the vial with the arrow on the meter.
5. Press the Meas key to begin the measurement.
6. Slowly rotate the vial by approximately 45 degrees. Close the light shield lid and record the measured value.
7. Repeat step 6 until the lowest turbidity reading is shown.
8. Mark this position on the vial with respect to the alignment arrow on the meter.
MATCHING SAMPLE VIALS
For the best accuracy and repeatability of turbidity measurements, use of one indexed vial is the best choice. If you need to use
several vials, matching of these vials is necessary.
1. Repeat the steps above for each vial and record the measured values.
2. Find the closest measuring value of these vials and mark its position with respect to the alignment arrow on the meter.

Addendum 2. Preparation of Standard Formazin Solutions
Preparation of turbidity-free water
Turbidity-free water is used for the preparation of turbidity standards and is prepared by filtering deionized water through
a 0.45µm or smaller pore-sized membrane.
Preparation of turbidity standards:
Turbidity Standard
Preparation Method
4000 NTU
Dissolve 1g hydrazine sulfate [(NH2)2·H2SO4] in the turbidity-free water and dilute to 100ml in a
volumetric flask.
Dissolve 10g hexamethylenetetramine [(CH2)6N4] in the turbidity-free water and dilute to 100ml
in a volumetric flask.
Mix 5ml of hydrazine sulfate and 5ml of hexamethylenetetramine solutions in a 100ml volumetric
flask and let stand for 24 hours at 25(±3) °C.
1000 NTU
Mix 25ml of 4000 NTU standard in a 100ml volumetric flask and dilute to the mark.
500 NTU
Mix 12.5ml of 4000 NTU standard in a 100ml volumetric flask and dilute to the mark.
200 NTU
Mix 10ml of 4000 NTU standard in a 100ml volumetric flask and dilute to the mark.
Mix 50ml of above standard in a 100ml volumetric flask and dilute to the mark.
10 NTU
Mix 10ml of 4000 NTU standard in a 100ml volumetric flask and dilute to the mark.
Mix 2.5ml of the above standard in a 100ml volumetric flask and dilute to the mark
OR mix 2ml of 500 NTU standard in a 100ml volumetric flask and dilute to the mark.
Warranty
The warranty period for the meter is one year from the date of shipment. Above warranty does not cover the calibration standards.
Out of warranty products will be repaired on a charged basis. The warranty on your meter shall not apply to defects resulting from:
●Improper or inadequate maintenance by the customer.
●Unauthorized modification or misuse.
●Operation outside of the environment specifications of the products.
For more information, please contact the nearest authorised distributor.
The information in this document is subject to change without notice. Copyright © Trace2O Limited, 2017. All rights reserved.

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