Tuttnauer LABSCI 11L CPVG User manual

Operation and Maintenance Manual
Electronic Laboratory Autoclaves
Models LABSCI 11L CPVG
First time published; 07.25.2017
ELG-D: standard autoclave
C: optional system for fast cooling
PV: optional vacuum pump
BH: optional bio hazard filtration
F: optional fan for super-fast cooling
Cat. No. MAN205-0480002EN Rev. H
Heidolph North America , 1235 N Mittel Blvd. Suite B Wood Dale IL 60191
Phone: [phone] Fax: [phone], e-mail: [email protected]

Page 1
Table of Contents
Paragraph page no.
1General..................................................................................................................4
1.1 Introduction .............................................................................................4
1.2 Incoming Inspection .............................................................................6
1.3 Warranty ...................................................................................................6
1.4 Warranty Statement .............................................................................7
1.5 Ordering Information ...........................................................................7
1.6 Accessories ...............................................................................................8
2Safety Instructions................................................................................................8
3Technical Data....................................................................................................10
3.1 Storage Conditions ..............................................................................10
3.2 Operating Conditions ..........................................................................10
3.3 Directives and Standards ..................................................................11
3.4 Electrical Data...........................................................................................11
3.5 Environment Emission Information ...............................................11
3.6 Water Quality ........................................................................................12
3.7 Specifications ........................................................................................13
3.8 Overall Dimensions ..............................................................................14
3.9 Electrical Data .......................................................................................14
3.10 Utilities ....................................................................................................15
3.11 Environment Emission Information ...............................................15
3.12 Construction ..........................................................................................15
3.13 Safety Features .....................................................................................16
3.14 Stickers Description ............................................................................16
3.15 Front View ..............................................................................................18
3.16 Rear View ................................................................................................19
4Control Panel......................................................................................................20
4.1 Control Panel Drawing .......................................................................20
4.2 Description and Functions of the Front Panel Keyboard ........21
4.3 Displayed Error Messages / Symbols ............................................22
4.4 Displayed operational messages / Symbols ...............................23
4.5 Description of Operation ...................................................................24
4.6 Leakage Test - optional for PV (pre-vacuum) models only ...25
4.7 Bowie and Dick Test - optional for PV (pre-vacuum)
models only ............................................................................................25
5Sterilization Programs........................................................................................26
5.1 Program 1: Glass ..................................................................................29
5.2 Program 2: Plastic ...............................................................................30

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5.3 Program 3: Liquid A .............................................................................31
5.4 Program 4: Liquid B –Waste ............................................................32
5.5 Program 5: Liquid A –Cooling (C –cooling models only) ......33
5.6 Program 6: Liquid B –Waste Cooling (C –cooling models
only) .........................................................................................................34
5.7 Program 7: Waste (PV –pre-vacuum models only) .................35
5.8 Program 8: Hollow Load (PV –pre-vacuum models only) .....36
5.9 Program 9: Bio Hazard 1 (BH, bio-hazard models only) .........37
5.10 Program 10: Bio Hazard 2 (BH, bio-hazard models only) ......38
5.11 Program 11: Bio Hazard Liquids (BH, bio-hazard models
only) .........................................................................................................39
5.12 Program 12: Vacuum Test (PV, pre-vacuum models only) ....40
5.13 Program 13: Bowie and Dick Test (PV –pre-vacuum
models only) ..........................................................................................41
5.14 Program 14: Warm-Up .......................................................................42
5.15 Program 15: Isothermal ....................................................................43
5.16 Program 16: Air Mixture ....................................................................44
5.17 Program 17: Glass Test ......................................................................45
5.18 Program 18: Durham ..........................................................................46
6Screens................................................................................................................48
6.1 Screens following a completely successful cycle –"Cycle
Ended" .....................................................................................................48
6.2 Screens following aborted cycles after complete
sterilization stage ................................................................................49
6.3 Screens following a fail cycle: .........................................................49
7Printer.................................................................................................................51
7.1 Printer Output .......................................................................................51
7.2 Printer Handling ...................................................................................53
8Installation..........................................................................................................56
8.1 Placing .....................................................................................................56
8.2 Lifting and carrying .............................................................................56
9Preparation Before Sterilization.........................................................................57
9.1 Instruments ...........................................................................................57
9.2 Wrapped Instruments ........................................................................58
9.3 Packs ........................................................................................................59
9.4 Tubing ......................................................................................................60
9.5 Liquids .....................................................................................................60
10 Operating Instructions........................................................................................61
10.1 Turning on the autoclave ..................................................................61
10.2 Opening the door .................................................................................61
10.3 Loading ....................................................................................................61
10.4 Operations ..............................................................................................62

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10.5 Unloading ................................................................................................63
10.6 Stopping the process and canceling the ERROR message .....64
10.7 Cycle by Clock mode (Start Cycle by Clock) ................................64
10.8 Moving the Autoclave .........................................................................64
10.9 Loading and Unloading the Device .................................................65
11 Checking and Changing Parameters and Other Data .......................................66
11.1 Browsing through the menus ...........................................................66
11.2 Changing a Parameter ........................................................................67
11.3 Quick options screen ...........................................................................69
11.4 Logging in and entering the Main menu ......................................73
11.5 Changing parameters .........................................................................74
11.6 System Parameters .............................................................................75
11.7 Maintenance ..........................................................................................76
11.8 Cycle parameters .................................................................................77
12 Service and Maintenance Instructions...............................................................79
12.1 Preventive and Scheduled Maintenance ......................................79
12.2 Draining the Reservoirs .....................................................................80
12.3 Cleaning the Air Jet .............................................................................82
12.4 Replacing the Door Gasket ...............................................................82
12.5 Checking the Safety Valve ................................................................83
12.6 Cleaning the water outlet strainer .................................................85
12.7 Water Sensor Cleaning .......................................................................86
13 Troubleshooting..................................................................................................87
14 Spare Parts List...................................................................................................92
15 Accessories..........................................................................................................92

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1General
Read the Operating Instructions carefully, before beginning any
operation on the autoclave!
1.1 Introduction
Model LABSCI 11L CPVG is a laboratory sterilizer designed especially
for the sterilization of instruments, liquids, and other materials in
hospital laboratories, laboratories & research institutes, food
laboratories and pharmaceutical facilities.
A computerized control unit ensuring a fully automatic sterilization cycle
controls the autoclave.
The PV (pre-vacuum) models are equipped with a vacuum pump.
The advantages of the pre-vacuum sterilizer in comparison to the
regular gravity displacement steam sterilizer are as follows:
•Removal of air pockets from packs and porous loads and most kinds of
tubes (rubber, plastic etc.) by vacuum at the first stage of the cycle.
•Better steam penetration into the load; resulting in effective
sterilization.
•Better temperature uniformity.
•Better drying of materials with closed doors due to the vacuum
achieved in the chamber at the end of the sterilization cycle.
In BH (bio-hazard) models, a bio-hazard filter installed in the chamber
enables sterilizing bio-hazard load without contaminating the
surrounding.
There is a configuration equipped with fan that allows shortening the
cooling operation.
The temperature and pressure are controlled through sensors placed
inside and outside the media container or bottles.
The autoclave is equipped with A 9Kw generator that produces the
steam required for the sterilization.
A special feature of the autoclave is the fast cooling stage for liquids. In
this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles.
A computerized control unit ensuring a fully automatic sterilization cycle
controls the autoclave.

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The temperature and pressure are controlled through sensors placed
inside and outside the media container or bottles.
The sterilizer is fully automatic with a choice of 10 programs,
eliminating any need for operator intervention during a cycle (program
7-10 are for vacuum test only). A computerized control unit enables
precise control and monitoring of physical parameters and clear
documentation of the sterilization cycles.
The autoclave is equipped with a safety valve, which will discharge at
an overpressure of over 2.8 Bar (40 psi), which is located on the
chamber near the pressure switch and the steam pressure gauge. The
control system provides adequate protection, to ensure the safety of
personnel and reliable operation with a minimum of down time.
The sterilizer has multiple built - in safety devices, which provides
adequate protection to ensure the safety of operating personnel.
The printer prints the preset and actual parameters of the cycle
(temperature, time and pressure).
The autoclave is provided with a pressure gauge that is used as guide
only. Should there be a power failure during the operation of the
autoclave, the pressure gauge indicates to the operator that there is
pressure in the chamber.
A deviation of +1.6% is accepted.
Note:
After operating the sterilizer, brown stains might appear on the bottom
of the chamber. These stains are a result of the heating elements that
are located at the lower external part of the chamber. The brown color
is a common phenomenon, can easily be removed, and will not have
any effect on the sterilized goods.
This manual is intended to give the user a general understanding of
how the autoclave works and indicates the best ways to operate and
take care of it in order to obtain optimum results and a trouble-free
operation. After reading this manual, operating the autoclave should be
straightforward. However, since the autoclave is built using high
technology sensitive components, no attempt should be made by the
user or any other unauthorized person to repair or recalibrate it.
Only technical personnel having proper qualifications, holding
technical documentation and adequate test instrumentation are
authorized to undertake repair or service.

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1.2 Incoming Inspection
The autoclave should be unpacked and inspected for mechanical
damage upon receipt. Observe packing method and retain packing
materials until the unit has been inspected. Mechanical inspection
involves checking for signs of physical damage such as: scratched
panel surfaces, broken knobs, etc.
If damage is apparent, contact your dealer or point of purchase, so that
they may notify the manufacturer and file a claim with the appropriate
carrier.
All Tuttnauer products are carefully inspected prior to shipment and all
reasonable precautions are taken in preparing them for shipment to
assure safe arrival at their destination.
1.3 Warranty
We certify that this instrument is guaranteed to be free from defects in
material and workmanship for one year against faulty components and
assembly with the exception of glassware, lamps and heaters.
The warranty does not include and does not replace routine
treatment and preventive maintenance to be performed according
to instructions in sec. 12.1 (Preventive Maintenance).
Our obligation is limited to replacing the instrument or parts, after our
examination, if within one year after the date of shipment they prove to
be defective. This warranty does not apply to any instrument that has
been subjected to misuse, neglect, accident or improper installation or
application, nor shall it extend to products that have been repaired or
altered outside the factory without prior authorization from us.
The Autoclave should not be used in a manner not described in
this manual!

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1.4 Warranty Statement
To activate your warranty or for warranty information on this unit please
contact your dealer or Heidolph at one of the #'s listed below:
Heidolph North America, 1235 N Mittel Blvd. Suite B Wood Dale IL
60191
Service & Repair Division
Troubleshooting, Service incidents, Warranty repair, Repair, Services
and Repair authorization
Phone: [phone] Fax: [phone], e-mail:
[email protected]
Tuttnauer USA Co., Ltd., 25 Power Drive Hauppauge, NY 11788, USA
[phone], [phone], Fax: [phone]
e-mail: [email protected].
Note:
If you have any questions or there are any difficulties with this
instrument and the solution is not covered in this manual, please
contact your dealer or Heidolph.
Do not attempt to service this instrument yourself.
If there is any difficulty with this instrument, and the solution is not
covered in this manual, contact our representative or us first. Do not
attempt to service this instrument yourself. Describe the difficulty as
clearly as possible so we may be able to diagnose the problem and
provide a prompt solution.
If the autoclave is equipped with a printer, send along a copy of the last
printout for our inspection. If replacement parts are needed, stipulate
the model and serial number of the machine.
No products will be accepted for repair without proper authorization
from us. All transportation charges must be paid both ways by the
owner. This warranty will be void if the unit is not purchased from an
authorized full service Tuttnauer dealer.
1.5 Ordering Information
Several items must be specified when ordering the unit from your dealer.
•The chamber diameter and chamber depth required
•Please specify the supply voltage available (i.e. 115v/208v; 1Ph/3Ph)
•The temperature scale needed (Celsius or Fahrenheit).
•The pressure scale needed (kPa or psi)

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1.6 Accessories
•Baskets –Different size baskets are available for these units. The
baskets are made of stainless steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
The baskets are made of stainless steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
•Stainless steel containers - Different size containers are available for
these units .
The containers are designed for sterilizing waste material. The
containers have vent holes along the upper rim.
2Safety Instructions
The autoclave has unique characteristics. Please read and understand
the operation instructions before first operation of the autoclave. The
following issues may require instructions guidance provided by the
manufacturer: how to operate the autoclave, the door safety
mechanism, the dangers involved in circumventing safety means, how
to ensure that the door is closed, and how to select a correct
sterilization program.
Make sure that you know where the main power switch is, where the
water cut-off valve is and where the compressed air disconnection
valve is located.
Autoclave maintenance is crucial for the correct and efficient function of
the device. We enclose a log booklet that includes maintenance
recommendations, with every device.
The weekly spore test is part of the preventive maintenance plan, along
with the annual validation of the sterilization processes that ensures
appropriate temperature dispersion within the chamber.
Never use the autoclave to sterilize corrosive products, such as: acids,
bases and phenols, volatile compounds or solutions such ethanol,
methanol or chloroform nor radioactive substances.
1. Never start using a new autoclave or a new steam generator, before
the safety, licensing and authorization department has approved it for
use.
2. All autoclave users must receive training in proper usage from an
experienced employee. Every new employee must undergo a training
period under an experienced employee.
3. A written procedure must be established for autoclave operation,
including: daily safety tests, seal inspection and door hinge
inspection, smooth action of the closing mechanism, chamber

Page 9
cleaning, prevention of clogging and preservation from corrosion,
what is permitted and what is prohibited for sterilization and choosing
a sterilization program.
4. Liquids may be sterilized only with the “liquids” program. The
container must be covered but not sealed. Sealed bottles may only be
sterilized using a special program. The bottle must be either Pyrex or
a Borosilicate glass bottle.
5. When sterilizing plastic materials, make sure that the item can
withstand sterilization temperature. Plastic that melts in the chamber
is liable to cause a great deal of damage.
6. Individual glass bottles may be placed within an appropriate container
that will be placed on a tray. Never place glass bottles on the floor of
the autoclave. Never fill more than 2/3 of the bottle volume.
7. On closing the autoclave's door, make sure it is properly locked
before activating.
8. Before withdrawing trays, wear heat resistant gloves.
9. Before opening the door, verify that there is no pressure in the
chamber.
10. Open the door slowly to allow steam to escape and wait 5 minutes
before you remove the load. When sterilizing liquids, wait 10 minutes.
11. Once a month, ensure that the safety valves are functioning, and
once annually a certified tester must conduct pressure chamber
safety tests.
12. Once annually, or more frequently, effective tests must be performed,
i.e., calibration and validation.
13. Examine the condition of assemblies on a regular basis. Make sure
there are no leaks, breaks, blockages, whistles or strange noises.
14. It is required to conduct maintenance operations as instructed.
15. Immediately notify the person in charge of any deviation or risk for the
proper function of the device.

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3Technical Data
3.1 Storage Conditions
Packed or unpacked, autoclave shall be retained in indoor
conditions!
3.2 Operating Conditions
•This device is for indoor use only!
•The sterilizer should be loaded only with autoclavable material!
•The environment shall not exceed an ambient temperature of 40ºC
and a relative humidity of 85% respectively.
•The operation altitude shall not be over 2000 meters (6561 feet)
(ambient pressure shall not be lower than 80 kPa (11.6 psi)).
The autoclave shall not be used in a manner not specified in
this manual!
Do not use the autoclave in the presence of dangerous gases.
Operate the autoclave only in the manner specified in the manual.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Caution!
Waste water should be brought into the public net in accordance
with the local rules or requirements i.e ONLY NON-HAZARDOUS
LIQUIDS SHALL BE DISPOSED IN PUBLIC SEWAGE!

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3.3 Directives and Standards
Tuttnauer. Ltd. company meets the provisions of the following standards:
EN ISO 13485:2012 (Quality Systems for Devices)
ISO 9001:2008 (Quality Systems)
MDD 93/42/EEC (Device Directive)
Tuttnauer. Ltd. company also works in conjunction with and refers to:
AAMI/ANSI-ST8 Hospital sterilizers
ASME American Society of Mechanical Engineers
Section VIII, Division 1, for unfired pressure vessels
EN 13060 Small Steam Sterilizers
UL UL 61010-1
PED 97/23EEC
IEC IEC 61010-2-040 Safety
IEC IEC 61326-1 EMC
ISO 17665-1:2006 (Validation and Routine Control)
3.4 Electrical Data
Property
Value
Total Power
9000W
Voltage
230VAC / 1ph
Amperage
10A
Protection against
electrical shock
Class I (IEC 60601-1)
Mains supply
fluctuation
+/- 10%
Degree of protection
by enclosure
IP31
Note: In order to avoid any injury by electrical hazard, it is recommended that
a ground fault protection device be installed in the electrical panel
feeding the autoclave (local codes may make this mandatory).
3.5 Environment Emission Information
A. Peak sound level generated by the sterilizer is « 70 / dBA with a back
sound level of 60 dBA.
B. Total heat transmitted by the sterilizer is < 150 W/h

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3.6 Water Quality
The distilled or mineral-free water supply shall be according to the table
below:
Physical Characteristics and Maximum acceptable contaminants levels
in water or steam, for steam generator and sterilizers
Contaminants in
water supplied to
generator/chamber
Contaminants in
condensate at steam
inlet to sterilizer
Evaporate residue
≤ 10 mg/l
N/A
Silicate (SiO2)
≤ 1 mg/l
≤ 0.1 mg/l
Iron
≤ 0.2mg/l
≤ 0.1mg/l
Cadmium
≤ 0.005 mg/l
≤ 0.005 mg/l
Lead
≤ 0.05 mg/l
≤ 0.05 mg/l
Rest of heavy metals
except iron,
cadmium, lead
≤ 0.1 mg/l
≤ 0.1 mg/l
Chloride (Cl)
≤ 2 mg/l
≤ 0.1 mg/l
Phosphate (P2O5)
≤ 0.5 mg/l
≤ 0.1 mg/l
Conductivity (at
25°C)
3 to 15 µs/cm
≤ 3 µs/cm
pH value (degree of
acidity)
5 to 7.5
5 to 7
Hardness (Σ ions of
alkaline earth)
≤ 0.02 mmol/l
≤ 0.02 mmol/l
Appearance
Colorless, clean, without sediments
Compliance with the above data should be tested in accordance with
acknowledged analytical methods, by an authorized laboratory.
Attention:
The use of water for autoclaves that do not comply with the table
above may have severe impact on the working life of the sterilizer
and can invalidate the manufacturer’s guarantee.
Use only deionized water, having a maximum conductivity of 15
µs/cm. Conductivity greater than 15 µs/cm may cause failures.
Tap water supply
The range of hardness value 0.7-2.0 mmol/l (70- 200 mg/l CaCO3)
The use of soft water is strictly forbidden!
Please consult a water specialist!

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Drain Cooling
The feed water supplied to the drain cooling must meet the following
requirements:
Hardness: 0.7 - 0.2 mmol/l.
Water temperature shall not exceed 15°C (59°F).
3.7 Specifications
Property
Value
Overall
dimensions
Height
440mm
Width
530mm
Length
760mm
Maximum
dimensions
(door open)
Width
1090mm
Distance
between
supporting
legs
Width
315mm (front legs), 400mm (rear legs)
Length
532mm
Weight per support area
(max. load)
According to overall weight and floor
loading requirements
Net weight
78kg
Shipping volume
0.48 m3
Shipping weight
100 kg
Shipping
dimensions
length
93
width
72
height
72
Max. Allowable Working
pressure (MAWP)
2.8 bar
Chamber
diameter
280mm
Depth
400mm
Chamber Volume
28.5 lit.

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3.8 Overall Dimensions
3.9 Electrical Data
Property
Value
Total Power
3000W
Voltage
230VAC / 1 ph
Amperage
10A
Protection against electrical
shock
Class I (IEC 60601-1)
Mains supply fluctuation
+/- 10%
Degree of protection by
enclosure
IP31
Note: In order to avoid any injury by electrical hazard, it is recommended that
a ground fault protection device be installed in the electrical panel
feeding the autoclave (local codes may make this mandatory).

Page 15
3.10 Utilities
Specifications
Value
Mineral free water
See table in sec. 3.9.
Power supply
* 1 phase, 230VAC ±10%, 60Hz
Recommended circuit
breaker
15A
Compressed Air (PV-
re-vacuum models
only)
1/2" 3 Bar (44-58 psi)
Tap water
1/2", 2-6 Bar (29-44 psi)
Mineral free water
1/2", 2-3 Bar (29-44 psi)
Drain
2" Minimum Withstanding temp. of
80°C (176°F)
* According to the local network.
Attention:
•A switch or circuit-breaker must be included in the building
installation. This switch or circuit-breaker shall be in close
proximity to the equipment, within easy reach of the operator; and
marked as the disconnecting device for the equipment.
•The electrical net must be protected with a current leakage safety
relay.
•The electrical network must comply with local rules or
regulations.
•Verify that there is an easy access to the main power switch, to
the water cut-off valve and to the current leakage safety relay.
3.11 Environment Emission Information
1. The peak sound level generated by the autoclave is 65dBa with
background noise of 48 dBa.
2. The total heat per hour transmitted by the autoclave is <200Wh.
3.12 Construction
The main parts of the autoclave are made of materials as indicated
below:
•Chamber is built of stainless steel.
•Door is made of stainless steel.

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•Trays are made of stainless steel.
•Water reservoir is made of hard plastic material.
•Door handle is made of hard plastic material, which is safe to touch
and thermo-insulated.
3.13 Safety Features
This autoclave includes built-in safety features such as:
•Error message display.
•Temperature dependent door locking system according to European
standards.
•Electronic pressure and temperature measurement.
•Safety relief valve to avoid build-up of excessive pressure.
•Door switch enabling operation to be started only when the door is
closed.
•Water level safety device.
•Excess temperature protection.
3.14 Stickers Description
Symbol
Meaning
Part Number
Location
Caution! Hot
steam.
LAB048-0058
Near the safety
valve
Protective earth
(Ground)
LAB048-0020
Near the earthing
screw
Caution! Hot
surface.
LAB048-0023
On the rear cover
above the water
strainer
Press & Hold
Until “System
Ready” is
Displayed = 10
sec.
LAB048-0461
(Automatic
door only)
On the door right
edge
Don’t Touch
Microswitches
please Contact
Tuttnauer
Service
LAB048-0462
On the door frame
behind the door
cover
Don’t Touch
Microswitches
please Contact
Tuttnauer
Service
LAB048-0463
On the door frame
behind the door
cover

Page 17
ON
OFF
On-Off
LAB048-0018
Near the Power
switch

Page 18
3.15 Front View
No.
Description
No.
Description
1
Main switch circuit
breaker
10
Door switch
2
Operating keyboard
11
Door closing device
3
Printer cover
12
Water reservoir funnel
4
Rear Leg
13
Water level gauge
5
Printer (option)
14
Autoclave cover
15
7
14
17
16
9
7
8
12
13
11
10
5
2
6
4
3
1

Page 19
6
USB connection
15
Display
7
Front Legs
16
Mineral-free water reservoir
cover
8
Mineral-free water
reservoir drain valve
17
Safety valve
9
Door cover
3.16 Rear View
No.
Description
No.
Description
1
Water reservoir cover
7
Tap water inlet
2
Compressed air inlet
8
Overflow drain
3
Compressed air pressure gauge
9
Chamber water strainer
4
Air pressure regulator
10
Main power electrical cable
socket
5
Air pump cover : optional for PV
(pre-vacuum) models only
11
Cut-off
6
Drain
9
1
6
7
8
3
2
4
5
10
11