UGO BASILE 37360 User manual

UGO BASILE S.R.L.
[email protected] / [e-mail] www. ugobasile .com
instruction manual
Tail-Flick Unit
Cat. No. 37360

UGO BASILE S.R.L.
Via Di Vittorio, 2
21036 GEMONIO, VA, ITALY
Phone: [phone]
instruction manual
Tail-Flick Unit
Cat. No. 37360
[email protected] / [email protected]
www. ugobasile .com
Serial No.
SAFETY CONSIDERATIONS
ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL SAFE-
TY STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND WARN-
INGS WHICH MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO RETAIN
THE INSTRUMENT IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSON-
NEL, AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UN-
DER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITA-
BLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE
HAZARD INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE IN-
STRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated July 2016
Revision 0

www.ugobasile.com
Tail-Flick Unit
General
This new style Tail Flick Unit has been designed to perform rapid precise screening of analgesic drugs via heat stimulation on the rat tail,
according to D’Amour & Smith, see bibliography. It basically consists of an I.R. source, whose radiant energy of adjustable intensity is
focused on the rat tail by an embodied parabolic mirror.
The rat is held by the operator on the instrument unobstructed upper panel (see picture) in such a way that its tail, placed over a ush
mounted window, receives the I.R. energy.
The operator starts the stimulus and the related solid state second counter. When the rat feels pain and icks its tail, a sensor detects it,
stops the second counter and switches o󱐯 the bulb. The reaction time of the animal is thus determinedand automatically recorded.
PAIN and INFLAMMATION
Dedicated Software
Cat. No. 37360
RAPID and PRECISE
SCREENING OF
ANALGESIC DRUGS
ON THE RAT TAIL
Main Features
l Automatic detection of the animal
response
l Data portable to USB pen-drive
stick or to PC (USB)
l Comfortable, unobstructed working surface (no
protruding elements)
l Excellent reproducibility thanks to optics lodged
in a rigid structure & electronically controlled I.R.
ux
Memory Key included
Ugo Basile: more than 10,000 citations

CHECK-LIST
Cat. No. 37360 Tail-Flick Unit
CLIENTE / CUSTOMER______________________________________________
Ordine No. / Order No. ____________________ Data / Date____/_____/______
UB code CAT.No.
 Q.ty DESCRIPTION DESCRIZIONE
37215-303 1 PEDAL SWITCH PEDALE
E-WP 008 1 MAINS CABLE EUROPE CAVO RETE EUROPA
E-WP 008-1 1 USA USA
E-FF 002 2 FUSES (2AF) FUSIBILI (2AF)
E-AU 041 USB
pen-drive 37360-302 1 INSTRUCTION MANUAL MANUALE DI ISTRUZIONE
52050-09 CUB SOFTWARE SOFTWARE CUB
52010-323 1 USB CABLE CAVO USB
OPTIONAL
37360-325 MOUSE HOLDER (25mm I.D.) CONTENITORE TOPO, DIAM. 25mm
37360-330 MOUSE HOLDER (30mm I.D.) CONTENITORE TOPO, DIAM. 30mm
DATE / / Serial No. IMBALLATO DA / PACKED BY
Universal Input 85-264 VAC, 50-60Hz
IMPORTANT/IMPORTANTE:
Check the shipment for completeness immediately after receipt: should you find any discrepancy, please fill in the following part and transmit it to
our fax no. [phone]
Al ricevimento della merce controllate che la spedizione sia completa: in caso di discrepanza, completate il formulario di seguito riportato ed in-
viatelo al nostro fax no. 0332 745488
FROM: Name Company/Institution
DATE REF.
NOTE
MOD.04 REV 0

Page i
37360 Instruction Manual (Rev. 0)
C O N T E N T S
1GENERAL ..........................................................................................................1
1.1PRINCIPLE OF OPERATION .....................................................................................1
2INSTRUMENT DESCRIPTION...........................................................................1
2.1THE COMMAND-DISPLAY MODULE..........................................................................2
2.1.1GRAPHIC DISPLAY 2
2.1.2KEYBOARD 3
2.1.3FUNCTION KEY ABBREVIATIONS 3
3DATA ACQUISITION & DATA PRINT-OUT ......................................................4
3.1DATA PORTABILITY ...............................................................................................4
3.257145 MINIPRINTER .............................................................................................4
4INSTALLATION .................................................................................................5
4.1UNPACKING &PRELIMINARY CHECK ......................................................................5
4.2NOTES ON THE INSTRUCTION MANUAL ....................................................................5
4.3ASSEMBLING THE EQUIPMENT................................................................................5
4.4ENVIRONMENT ......................................................................................................6
4.5BEFORE APPLYING POWER....................................................................................6
4.5.1FUSE HOLDER 6
4.5.2MAIN SWITCH 6
4.5.3MAINS CORD 7
4.6CONNECTIONS ......................................................................................................7
4.6.1BACK PANEL 7
4.7INSTALLATION OF CUB SOFTWARE ........................................................................8
4.8INTENDED USE ......................................................................................................8
4.9GENERAL SAFETY INSTRUCTIONS...........................................................................8
4.10ADDITIONAL SAFETY CONSIDERATION ....................................................................8
5OPERATION ......................................................................................................9
5.1SWITCHING ON.....................................................................................................9
5.2DISPLAY CONTRAST..............................................................................................9
5.3SETTING I.R. INTENSITY.........................................................................................9
5.4INITIAL “DRY RUN”.............................................................................................10
5.5HOLDING THE ANIMAL .........................................................................................10
5.5.1MOUSE RESTRAINER 10
5.6CARRYING OUT THE TEST ON THE ANIMAL.............................................................10
5.6.1NOTE ON WET SURFACES 11
5.7HOW TO SET A CUSTOMIZED EXPERIMENTAL CONFIGURATION ...............................11
5.7.1SEQUENCE NUMBER 11
5.7.2EXPERIMENT NUMBER 12
5.7.3ANIMAL GENDER 12
5.7.4CUT-OFF TIME 12

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37360 Instruction Manual (Rev. 0)
5.8CONFIGURATION FILE ......................................................................................... 13
5.8.1FROM THE MEMORY KEY TO THE INSTRUMENT 13
5.8.2UPLOADING 14
5.8.3DOWNLOADING 14
5.8.4FROM MEMORY KEY TO PC 14
5.8.5FROM PC TO INSTRUMENT 14
6OPERATING WITH MINI PRINTER AND PC.................................................. 14
6.1HOW TO SET THE DATA OUTPUT TO PC ................................................................ 14
6.2HOW TO SET THE DATA OUTPUT TO MINIPRINTER.................................................. 15
6.3DATUM FORMAT ................................................................................................. 15
6.4QUICK SETTING OF THE 37360 TAIL FLICK UNIT ................................................... 16
7MEMORY MANAGEMENT .............................................................................. 17
7.1MEMORY RESET ................................................................................................. 18
7.1.1“MEMORY FULL”MESSAGE 19
7.2SETTING TIME &DATE ........................................................................................ 19
8REPRODUCIBILITY OF THE TEST ................................................................ 20
9CALIBRATION ................................................................................................ 20
9.1RADIOMETER...................................................................................................... 20
9.2CALIBRATION VIA THE 37300 I.R. RADIOMETER.................................................... 21
9.3CALIBRATION WITHOUT I.R. RADIOMETER ............................................................ 22
10MAINTENANCE............................................................................................... 22
10.1ELECTRICAL....................................................................................................... 22
10.1.1BATTERY REPLACEMENT 23
10.2REPLACING THE BULB ........................................................................................ 24
10.3CLEANING THE OPTICS THOROUGHLY .................................................................. 24
10.4LONG INACTIVITY................................................................................................ 24
10.5CUSTOMER SUPPORT ......................................................................................... 25
11INSTRUMENT SPECIFICATIONS................................................................... 25
12ORDERING INFORMATION............................................................................ 26
12.1SPARE PARTS .................................................................................................... 26
12.2OPTIONAL .......................................................................................................... 26
12.3THERMAL MINIPRINTER....................................................................................... 27
13BIBLIOGRAPHY.............................................................................................. 27
13.1METHOD PAPER ................................................................................................. 27

13.2PAPERS MENTIONING UGO BASILE TAIL FLICK UNIT..............................................27
14APPENDIX .........................................................................................................1
FIGURE INDEX
Figure 1 “Front Panel and USB slot”..................................................................................2
Figure 2 “Top Panel”..........................................................................................................5
Figure 3 “Power Module”...................................................................................................6
Figure 4 “Back Panel”........................................................................................................7
Figure 4 “Back Panel”......................................................................................................10
Figure 6 “Acquired Data Window”....................................................................................16

Page 1
37360 Instruction Manual (Rev. 0)
Tail Flick Unit
Cat. 3 7 3 6 0
1 GENERAL
This new style Tail Flick Unit has been designed to perform rapid precise screening of
analgesic drugs on the rat tail, according to D'Amour & Smith, see BIBLIOGRAPHY, par-
agraph 13.1-Method Paper.
The instrument measures the latency of the avoidance response when pain is induced by
radiant heat applied to the animal’s tail.
Its main features are:-

 Direct connection to a PC (USB and serial)

 Adjustable I.R. Intensity

 Multifunction graphic display readout

 USB pen-drive provided with the standard package

 No protruding elements: flush mounted infrared window

 Comfortable, unobstructed working surface

 No stray radiation
1.1 Principle of Operation
The rat is held by the operator on the instrument unobstructed upper panel (see 1, figure
1, figure 2, and datasheet picture) in such a way that its tail, placed over a flush mounted
window, receives the I.R. energy.
The operator starts the stimulus and the related reaction-time counter by the pedal switch
or by function key located on the front panel.
When the rat feels pain and flicks its tail, a sensor detects it, stops the reaction-time
counter and switches off the bulb. The reaction time of the animal is thus automatically
determined to the nearest 0.1 second.
2 INSTRUMENT DESCRIPTION
The Tail Flick Unit basically consists of an I.R. source, whose radiant energy of adjusta-
ble intensity is focused by an embodied parabolic mirror on the rat tail.

Page 2
37360 Instruction Manual (Rev. 0)
The instrument compo-
nents are neatly arranged
in a box of new design,
which contains the I.R.
source, the sensor, the mi-
crocontroller and the elec-
tronic circuit.
The instrument front panel
encompasses the Com-
mand-Display Module (2),
see also paragraph 2.1
and the slot for the
memory key (3), see also
paragraph 3.1-Data Porta-
bility3.1.
Figure 1 “Front Panel and USB slot”
The back panel embodies the power module, see paragraph 4.5, and the connection
module, see paragraph 4.6.
An inclined Mouse Restrainer is supplied as optional, to be used with the mouse to com-
pensate for its tendency to hold its tail at 45 degrees up and therefore away from the heat
source. See also 5.5.1Mouse Restrainer.
2.1 The Command-Display Module
This module of original design is located on the instrument front panel, see figure 1, and
comprises a graphic display and a command keyboard.
The multifunction liquid-crystal graphic-display monitors the withdrawal latency to the
nearest 0.1 second; latency time is the time elapsing from the moment the START key is
activated to the moment the animal flicks its tail.
The graphic display presents all available command: the operator sets the experiment
configuration via the command keyboard located on the right of the display.
2.1.1 Graphic Display
When the instrument is switched on, our
logo with the two mice is displayed.
By depressing F4 (ESC), you enter the user
area.
3
2
1

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37360 Instruction Manual (Rev. 0)
The four lines of the top area show:
1) the actual date
2) the actual time
3) the instrument serial number
4) the firmware version
In all menus, the last two lines show the functions available by depressing the corre-
sponding key on the right of the display.
The right portion of the display window
shows the abbreviation of the key function,
meaning:
 OPR Operation
 FNC Function
 MEM Memory
 ESC Escape
The display contrast can be modified, see paragraph 5.2-Display Contrast.
2.1.2 Keyboard
The keyboard, consisting of 4 soft push-button keys F1, F2, F3 and F4, sets the experi-
ment parameters, see paragraph 2.1.3 for reference.
2.1.3 Function Key Abbreviations
CAL Calibration
CFG Configuration
CLK Clock setting
CUT Cut-off time
DIS Display contrast
DWN Decrement
ENT Enter
EPR External printer
ESC Escape
FNC Function menu
FTR Feature menu
IR I.R. intensity
MEM Memory menu
MPR MiniPrinter
MSK Memory stick
NO No
NUL Annulment
OK Confirm
OPR Operation menu
OUT Out
PRN Data printing selection
RES Data memory reset
STR Start
STP Stop
The F4 key, which has the
function of ESCAPE key, en-
ables the operator to leave a
menu and go back to a pre-
viousl
y
selected one.

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37360 Instruction Manual (Rev. 0)
UP Increment
V.W Data view
YES Yes
> Shift to right
3 DATA ACQUISITION & DATA PRINT-OUT
The Tail Flick Unit is a microprocessor controlled unit. The experimental data, stored in
the 37360 internal memory and shown on its graphic display, can be directly exported to
PC.
The USB connector type B connects directly into the PC USB port. This connector is lo-
cated on the instrument back panel (see paragraph 4.6.1). The connection to PC is also
possible via the serial port RS 232 C by the Delta 9-pin female connector (COM 1).
Communication is managed by the dedicated CUB Package Cat. 52050-09, supplied as
standard.
The CUB 52050 is a Windows® based Data Acquisition Software Package, which ena-
bles the research worker to route to the PC the data originated by all Ugo Basile instru-
ments and store them into individual files, ready to be easily managed by most statistical
analysis packages available on the market. See also paragraph 4.7.
Ask for details!
3.1 Data Portability
Data portability is arranged via the USB pen-drive (E-AU 041) provided with the stand-
ard package. Via the USB slot provided on the front panel, all the experimental data of
one or more sessions can be easily uploaded on the pen-drive, see 1, Figure 1, in para-
graph 2.
Programming the experiment layouts from a remote PC is also made easy by the use of
the pen-drive.
3.2 57145 MiniPrinter
The MiniPrinter 57145 is a compact thermal printer device, supplied as an optional part,
suitable to print experimental data on 58mm wide paper, see paragraph 12.3-Thermal
MiniPrinter
See paragraph 4.6 for connection to the Tail Flick Unit via the 10-pin cable provided with
the Mini-Printer and paragraph 6.2 to enable the data print-out on the MiniPrinter.
For further details, please refer to MiniPrinter Instruction Manual.

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37360 Instruction Manual (Rev. 0)
4 INSTALLATION
4.1 Unpacking & Preliminary Check
Check the contents of the shipment for completeness, packing list to hand, and visually
inspect the instrument as soon you take it out of the packaging. Use the supplied Check
List. The 37360 functional design which avoids protruding elements is the best defence
against rough transport handling.
If the instrument is damaged, inform the carrier immediately, notifying our company. If af-
ter having tested it, the Tail Flick Test fails to meet rated performances, please contact
our after sales service, see paragraph 10.5-Customer Support.
Protect the environment!
Dispose of packaging properly, according to existing and ap-
plicable waste management rules and regulations.

4.2 Notes on the Instruction Manual
The Tail Flick Instruction Manual included in the package (on the USB pen-drive) is nec-
essary for the correct installation and operation of the instrument.
We recommend reading the manual with attention, as it is essential for the correct instal-
lation and operation of the instrument.
Please save the manual, ready to be consulted by the qualified personnel who use the
instrument. Print it, only if necessary.
Our Instruction Manuals are available as free download on our web, see
http://www.ugobasile.com/support/documentation.html. For any additional information
and/or assistance, you are welcome to contact our Service Department (see paragraph
10.5-Customer Support), specifying the serial number of your instrument.
4.3 Assembling the Equipment
Position the Tail Flick on a stable and flat
bench or table surface.
Fit the top panel extension, as indicated in
figure 2, and secure it by tightening the two
screws.
Figure 2 “Top Panel”

Page 6
37360 Instruction Manual (Rev. 0)
4.4 Environment
Avoid direct illumination of the enclosure compartments, e.g., from a
table lamp.
This will definitely upset the animals which dislike full light, not to speak of the risk to
raise their body temperature.
Moreover, intense light may cause the sensor to miss the tail removal, as a light
source from above tends to mask the I.R. energy reflected back by the paw.
We recommend absorbing with cotton wool any occasional large emission of urine during
familiarization or test time. The tail skin, if wet by urine, will be definitely less sensitive to
the thermal stimulus because of the large specific heat of any watery solution, see para-
graph 5.6.1.
4.5 Before Applying Power
Consider the Power Module on the back panel of the Tail Flick, which includes (from left
to right):


the inlet connector of the
mains cord


the mains switch


the fuse holder
Figure 3 “Power Module”
4.5.1 Fuse Holder
The instrument is provided with 2 fuses, on both neutral and live. To replace fuses, if
necessary, pull out the fuse slide, see paragraph 10.1-Electrical.
It is recommended to use fast-blow fuses type F2A; make sure that only fuses with the
required rated current and of the specified types are used for replacement. The use of
repaired fuses and the short circuiting of the fuse holders must be avoided.
4.5.2 Main Switch
This two-pole toggle switch, which complies with international safety standards, provides
a visual cue, to signal the:
- OFF position by O
- ON position by I

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37360 Instruction Manual (Rev. 0)
4.5.3 Mains Cord
It is a standard cable, Cat. # E-WP008, see paragraph 12-ORDERING INFORMATION.
Make sure your power outtake is provided with a reliable ground connection.
4.6 Connections
Connect the mains cord to a power outtake, provided with a reliable earth connection,
protected by a differential earth-leakage switch (CGFI) that breaks at the threshold of
0.03A, within a max. acceptable delay of 1s. The maximum breaking compliance required
by the CGFI is 10kA at rated current is 6A.
4.6.1 Back Panel
Beside the mains module, the back panel embodies a connection module, see figure be-
low:
Figure 4 “Back Panel”
The connection module encompasses, from left to right:
USB this USB type B connector for linking the Tail Flick to the USB port of the PC
COM1 a delta 9-pin female connector for connection to the serial port RS232C of the
PC
COM2 a second delta 9-pin connector (male) for connection to an external serial
printer
MINI
PRINTER a 10-pin connector to the Mini Printer Cat. 57145, see also (see paragraphs
3.2 and 12.3
PEDAL
SWITCH a 4-pin circular connector for the pedal switch. Insert the male connector of the
pedal switch cable until it locks into its socket; a “click” confirms it is locked.
Mismatching the connection is prevented by the different individual pin arrangement.

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37360 Instruction Manual (Rev. 0)
4.7 Installation of CUB software
The CUB software is contained in the USB pen-drive provided with the instrument. Install
it on your PC.
At the first installation, a code will be generated: send the code to registra-
[email protected] to receive an activation key.
From the installation, you have 30 days to enter the activation key; when the 30 days trial
period expires, it will not be possible to activate the software and it will be necessary to
uninstall and install it again (ask for instruction at our Customer Support, see paragraph
10.5).
4.8 Intended Use
The Tail Flick Unit is intended for investigation use on laboratory animals only.
4.9 General Safety Instructions
The following guidelines must be followed to ensure safe operation.
 DO NOT attempt to open or perform any service work
 DO NOT connect up human subjects
4.10 Additional Safety Consideration
a. Place your Tail Flick on a flat surface;
b. Do not obstruct free and comfortable access to the power module;
c. Use original accessories and spare parts only, see also paragraph 12-
ORDERING INFORMATION
d. Immediately disconnect and replace a damaged mains cable;
e. Do not operate the instrument in hazardous environments or outside pre-
scribed environmental limitations (i.e. +18C°/+24C°, 60% relative humidity,
non-condensing);
f. Do not spray any liquid on the connectors;
g. Keep inflammables far from the heating source.
UGO BASILE DOES NOT ACCEPT ANY RESPONSIBILITY FOR PROBLEMS OR
HARM CAUSED TO THINGS OR PERSONS, ARISING FROM:
 incorrect electrical supply;
 incorrect installation procedure;
 incorrect or improper use or, in any case, not in accordance with the purpose
for which the instrument has been designed and the warnings stated in the in-
struction manual supplied with the instrument;

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37360 Instruction Manual (Rev. 0)
 replacement of original components, accessories or parts with others not ap-
proved by the manufacturer;
 servicing carried out by unauthorized personnel
5 OPERATION
5.1 Switching On
Switch on the 37360; the software takes some seconds to perform a complete checking
of the electronic circuit. During the checking, the display shows the mice logo. By de-
pressing ESC, the display shows the software version which is actually installed, e.g.,
“V1.0”, see paragraph 2.1.1.
The software version is necessary in case of communication with our laboratory for oper-
ational or servicing problems.
In case the captions MEMORY LOSS!! appears on the display, please refer to paragraph
10.1.1-Battery Replacement.
5.2 Display Contrast
To modify the display contrast, depress the F2 (FNC) key from the Main Menu, then the
F3 (DIS) key. The display shows:
Keep the arrow keys F1 (UP ) or F2 (DWN)
depressed until you reach the desired dis-
play contrast.
The readability of the liquid crystal display
depends on the angle of view: select the
ideal contrast according to the height of the
table, the operator’s distance and so on.
5.3 Setting I.R. Intensity
Before carrying out the test on the animal, set the I.R. intensity. Enter the I.R. setting
menu, by depressing F1 (OPR), F2 (SET) and F1 (IR ).
The following display will appear:
Now use the F1 (UP ) or F2 (DWN) keys to set intensity to the desired value.

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37360 Instruction Manual (Rev. 0)
5.4 Initial “Dry Run”
We suggest to make an initial “dry run” to realize how the whole works, without the pres-
ence of the animal which will inevitably catch all available attention, in particular before
the operator masters how to position it.
Switch on! Use a strip of white paper, put it as to cover the I.R. window and depress the
pedal switch (momentary action is sufficient). This will turn on the I.R. source and start
the second counter (in 0.1 s steps).
Move away the strip: the bulb is turned off and the counter stops, providing a digital read-
out of the reaction time.
5.5 Holding the Animal
It is advisable to gather some experience in holding the rat/mouse on the top panel.
The animal must be kept firmly but not to the point of causing it a too severe discomfort: it
should in fact flick its tail when the heating reaches the threshold of pain and not as a
consequence of its struggling to slip out from the operator’s hand.
Some researchers roll up a cloth around the rat, leaving the tail protruding, a procedure
that is in most cases satisfactory.
5.5.1 Mouse Restrainer
The availability of mice with specific gene knockout is driving a substantial shift from rats
to mice as a research animal of first choice.
Inclined Mouse Restrainers are available
as optional, to be used with the mouse,
to compensate for its tendency to hold
its tail at 45 degrees up and therefore
away from the heat source.
Two restrainers are available:

 37360-325, I.D. 25 mm

 37360-330, I.D. 30 mm
See also paragraph 12.2-Optional.
Figure 5 “Back Panel”
5.6 Carrying out the Test on the Animal
Repeat the experiment with the animal. Position the animal so that its tail is over the I.R.
window. A trial is commenced by depressing the pedal switch or F1 (STR) on the front
panel, which activates the I.R. source and digital counter.

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37360 Instruction Manual (Rev. 0)
When the animal feels pain and flicks its tail, the I.R. source switches off and the reaction
time counter stops. The withdrawal latency to the nearest 0.1 s is automatically deter-
mined.
The datum shown on the display can be printed on the MiniPrinter or routed to the PC,
see paragraph 6-OPERATING WITH MINI PRINTER AND PC.
5.6.1 Note on Wet Surfaces
We recommend absorbing with cotton wool any occasional large emission of urine during
familiarization or test time. The tail skin, if wet by urine, will be definitely less sensitive to
the thermal stimulus because of the large specific heat of any watery solution.
Be patient and dry the upper panel pane before the first test and at least between each
test.
5.7 How to Set a Customized Experimental Configuration
This function enables the operator to personalize the basic datum by adding, in se-
quence:
 sequence number (see 5.7.1)
 experiment number (see 5.7.2)
 animal gender (see 5.7.3)
From the main menu, depress the F2 (FNC) key, followed by the F1 (OUT) key. Depress
again the key F2 (FTR); the display shows the first setting, the sequence number.
All the available selections for each additional information are displayed in a loop. Scan
them by the F1 (UP ) or F2 (DWN) keys, select the suitable one and confirm the selection
by depressing the F4 (OK) key, or avoid one or more features by depressing the F3
(NUL) key.
The selected features will be maintained even if the instrument is turned off. To erase
one or more features previously set, simply enter this software level, highlight the con-
cerned feature and depress the F3 (NUL) key.
After entering the last feature (M or F), the software goes automatically back to the main
menu.
5.7.1 Sequence Number
The first additional information to be de-
fined is the sequence number. This section
is highlighted as soon as the F2 (FTR) key
is depressed.
Use the F1 (UP ) or F2 (DWN) key to set
the desired sequence number from 01 to
99 and confirm by depressing F4 (OK).

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37360 Instruction Manual (Rev. 0)
The sequence number will automatically increase by one figure at any subsequent
measurement. It trips once a datum has been acquired and recorded, indicating the fresh
sequence number ready for the next measurement.
If a sequence number is not necessary, depress the F3 (NUL) key.
IMPORTANT:
When a fresh experimental session is begun, the sequence number can be
carried on from the number reached at the end of the previous trial or a new
number can be entered, following the instructions outlined above, for exam-
ple starting from 01.
5.7.2 Experiment Number
As soon the F4 (OK) or F3 (NUL) keys are
depressed, followed by the F4 key again,
the second option is highlighted.
The experiment number, from 1 to 9, can
now be selected via the F1 (UP ) or F2
(DWN) key and confirmed via the F4 (OK)
key.
IMPORTANT:
The experiment number is also used to label the data export file on the USB
pen-drive (for example: DATA01.txt, where the experiment number is 1, DA-
TA02.txt, where the experiment number is 2, etc.
5.7.3 Animal Gender
After the experiment number, it is possible
to define the gender of the animal to be
processed: MALE/FEMALE.
Select it by depressing the F1 (UP ) or F2
(DWN) key and confirm it via the F4 (OK)
key; avoid the gender selection by depress-
ing the F3 (NUL) key.
5.7.4 Cut-Off Time
To get over a “false start”, i.e., in case the beam, misaligned with the aimed tail area, has
been accidentally triggered, and to avoid useless overheating of the tail when the animal
does not react within the expected reaction time, a cut-off time can be set.
The preset value indicates the time (in seconds) after which the experiment will be inter-
rupted.

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37360 Instruction Manual (Rev. 0)
Select the cut-off time by depressing the
F1 (OPR), F2 (SET) and F2 (CUT).
Depress the F1 (UP ) or F2 (DWN) key un-
til you reach the desired value and confirm
it via the F4 (OK ) key.
5.8 Configuration File
Up to 6 different experiments can be configured, following the instructions outlined in
previous paragraphs, saved in the memory key, ready to be recalled to the PC and/or to
the instrument.
The involved parameters are:
 I.R. Intensity
 Cut-Off Time
 Experiment Number
 Gender
 Sequence Number
 MiniPrinter
5.8.1 From the Memory Key to the Instrument
With the memory key inserted in its slot on
the front panel, depress F1 (OPR), F2
(SET) and F3 (CFG).
The “READING CONFIGURATION” window ap-
pears; after some seconds, select the de-
sired configuration by the F1 (UP ) or F2
(DWN) key, then confirm the selection by
the F3 (OK ) key.
Under the configuration name, for example
“CONFIG01.CFG”, the related date appears
(save date).
If the configuration is not present, the cap-
tion “NOT PRESENT” appears instead of the
date.