UGO BASILE 47650 User manual

UGO BASILE S.R.L.
[email protected] / [email protected] www.ugobasile.com
instruction manual
Rotarod Mouse
Cat. No. 47650
E C T Unit
Cat. No. 57800

UGO BASILE S.R.L.
Via Di Vittorio, 2
21036 GEMONIO, VA, ITALY
Phone: [phone]
instruction manual
Rotarod mouse
Cat. No. 47650
[e-mail] / s[email protected]
www.ugobasile.com
Serial No.
SAFETY CONSIDERATIONS
ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL
SAFETY STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND
WARNINGS WHICH MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO
RETAIN THE INSTRUMENT IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED
PERSONNEL, AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT
UNDER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN
INEVITABLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF
THE HAZARD INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE
INSTRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated March 2021
Revision 2.2

CHECK-LIST
47650 Mouse Rota-Rod
CLIENTE / CUSTOMER______________________________________________
Ordine No. / Order No. ____________________ Data / Date____/_____/______
UB code
CAT.No.
Q.ty

DESCRIPTION
DESCRIZIONE
E-WP 008
MAINS CABLE
EUROPE
CAVO RETE
EUROPA
E-WP 008-1
U.S.A.
U.S.A.
47650-320
5
TRIP BOX
VASCHETTA
E-FT 013
2
FUSE (T2A)
FUSIBILE (T2A)
E-AU 041 USB
pen-drive
47650-302
1
INSTRUCTION MANUAL
MANUALE DI ISTRUZIONE
X-PAD
SOFTWARE
SOFTWARE
DATE / /
Serial No.
IMBALLATO DA / PACKED BY
Set for
115V 50 Hz
230V 50 Hz
115V 60 Hz
230V 60 Hz
IMPORTANT/IMPORTANTE:
Check the shipment for completeness immediately after receipt: should you find any discrepancy, please fill in the following part and transmit it
to our fax no. [phone]
Al ricevimento della merce controllate che la spedizione sia completa: in caso di discrepanza, completate il formulario di seguito riportato ed
inviatelo al nostro fax no. 0332 745488
FROM: Name
Company/Institution
DATE
REF.
NOTE
MOD.04 REV 0

Page i
47650 Instruction Manual (Rev. 2.1)
C O N T E N T S
1GENERAL .............................................................................................................1
1.1 PRINCIPLE OF OPERATION........................................................................................ 1
2INSTRUMENT DESCRIPTION.............................................................................1
2.1.1 NOTES ON RESISTIVE TOUCH-SCREENS 3
2.2 ROTA-ROD FEATURES .............................................................................................3
2.3 EXPERIMENTAL CONFIGURATION.............................................................................. 4
2.4 DATA COLLECTION AND MANAGEMENT ....................................................................4
3INSTALLATION ....................................................................................................4
3.1 UNPACKING &PRELIMINARY CHECK ........................................................................4
3.2 NOTES ON THE INSTRUCTION MANUAL......................................................................5
3.3 GENERAL SAFETY INSTRUCTIONS............................................................................. 5
3.4 ASSEMBLING THE INSTRUMENT ................................................................................5
3.5 BEFORE APPLYING POWER......................................................................................5
3.5.1 MAINS SWITCH 6
3.5.2 FUSE HOLDER 6
3.5.3 MAINS CORD 6
3.6 INTENDED USE.........................................................................................................6
3.7 ADDITIONAL SAFETY CONSIDERATION......................................................................6
3.8 CONNECTIONS .........................................................................................................7
3.8.1 USB HARD DISK 7
3.8.2 KEYBOARD 7
3.9 INSTALLATION OF X-PAD SOFTWARE.......................................................................7
4PRELIMINARY...................................................................................................... 7
4.1 PLACING THE MICE ON THE ROTOR...........................................................................7
4.2 ADJUSTING THE DETECTION SENSITIVITY..................................................................9
4.3 TRAINING................................................................................................................. 9
5OPERATION ......................................................................................................... 9
5.1 HOME PAGE ..........................................................................................................10
5.1.1 SYSTEM ICONS 10
5.1.2 HOME PAGE ICONS 10
5.2 EXPERIMENT.......................................................................................................... 11
5.3 SET-UP................................................................................................................. 12
5.3.1 CONSTANT SPEED 12
5.3.2 ACCELERATED/DECELERATED SPEED 12
5.3.3 REVERSE ROTATION 13
5.3.4 CUSTOM RAMP 13
5.3.5 ROCKING 14
5.4 START BUTTON...................................................................................................... 14
5.4.1 STARTING CONSTANT SPEED 14

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47650 Instruction Manual (Rev. 2.1)
5.4.2 STARTING A RAMP 15
5.4.3 STARTING A REVERSE ROTATION 16
5.5 RESULTS............................................................................................................... 18
5.6 USB STORAGE...................................................................................................... 18
5.6.1 SAVE DATA 19
5.6.2 LOAD PROTOCOL 19
5.7 UTILITIES............................................................................................................... 19
5.7.1 SOFTWARE UPDATE 20
5.7.2 SETTING DATE &TIME 21
5.7.3 DIAGNOSTICS 21
5.7.4 RESET HD 22
5.8 SIGNAL OUTPUT .................................................................................................... 22
5.8.1 PIN-OUT 22
6MAINTENANCE.................................................................................................. 23
6.1 ELECTRICAL .......................................................................................................... 23
6.2 CLEANING ............................................................................................................. 23
6.3 LONG INACTIVITY................................................................................................... 24
6.4 CUSTOMER SUPPORT ............................................................................................ 24
747650 SPECIFICATIONS................................................................................... 24
8ORDERING INFORMATION.............................................................................. 25
8.1 OPTIONAL ............................................................................................................. 25
9BIBLIOGRAPHY................................................................................................. 26
9.1 METHOD PAPERS................................................................................................... 26
9.2 PAPERS DEALING WITH UGO BASILE MOUSE ROTA-ROD....................................... 26
FIGURE INDEX
Figure 1 “Main Menu”..............................................................................................................2
Figure 2 “Trip-Box”..................................................................................................................3
Figure 3, "Power Module”........................................................................................................5
Figure 4, "Connection Module”................................................................................................8
Figure 5 “Placing the Mice on the Rotor”.................................................................................8
Figure 6 “Detection Sensitivity” ...............................................................................................9

Page 1
47650 Instruction Manual (Rev. 2.1)
Mouse Rota-Rod NG
Cat. 4 7 6 5 0
1 GENERAL
The “Rota-Rod” technique has been originated by a 1957 paper of N.W. Dunham and
T.S. Miya (see paragraph 9.1-Method Papers) and has proved to be of great value in
research involving screening of drugs which are potentially active on motor co-ordination.
Ugo Basile designed the first industrial Rota-Rod in the 1960s. The name we coined
soon became so popular, now everybody knows this instrument as RotaRod!
The Rota-Rod is the reference test to screen drugs potentially active, or having side
effects, on motor coordination.
The 47650 Rota-Rod NG (Next Generation), is an evolution of the original model and the
result of many years of research in cooperation with the latest development in behavioral
and pharmacological research.
1.1 Principle of Operation
When a mouse falls off its cylinder section onto the trip-box below, the plate boxes and
the corresponding magnetic switch is activated, thereby recording the animal endurance
time in seconds.
The display shows the actual angular speed (RPM). At the end of a run, the display
shows for each animal the running time, the rotation mode and the rotation speed at the
time the animal fell off, combined with information preset by the user.
The record related to each lane can be zeroed during the operation by resetting the
related trip plate. This allows the operator to place a fresh mouse on a particular rotor
lane while the mice on the other lanes are running.
2 INSTRUMENT DESCRIPTION
The 47650 combines the same functionality of the previous version, now considered the
standard, with additional new features: surprisingly silent operation, much easier
experimental organization and data management.
The Mouse Rota-Rod consists of five 3cm diam. cylinders, which are suitably machined
to provide grip. Six 25cm diam. dividers make for five lanes, each 5.7cm wide,
enabling five mice to be on the rotor simultaneously. The height to fall is 16cm.

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47650 Instruction Manual (Rev. 2.1)
The rotor, whose angular speed can be preset by the operator, turns on ball bearings. It
is driven by a silent D.C. geared motor. Drive speed is unaffected by voltage variations,
friction, or wear. This ensures that all trials can be repeated in constant operating
conditions. The standard rod rotation is CCW.
The 47650 incorporates a 4”3
touch-screen, for basic setting
and monitoring, via an intuitive
panel.
The picture shows the Rota-Rod
main menu.
Figure 1 “Main Menu”
During the test, the touch-screen
indicates the actual speed (RPM)
and the status of the rotor
section.
The test results for each section,
can be browsed in a condensed
view.
The brand new X-PAD software, included as standard, provides a user-friendly interface
to set the experiment and a powerful tool to manage the results easily on one’s PC. Data
exchange from the instrument to the PC is done via the USB flash drive provided.

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47650 Instruction Manual (Rev. 2.1)
See also paragraphs 2.3-Experimental Configuration and 2.4-Data Collection and
Management.
2.1.1 Notes on Resistive Touch-Screens
If you have in mind the sensitivity to touch of your tablet or smart-phone, you might think
the Rota-Rod touch screen does not react promptly, and you have to press down harder.
This is because we employ a resistive touch-screen (while most smart phones use a
capacitive screen); the resistive screens are better of our application because of
▪High resistance to dust and water
▪Better use with gloved hand or stylus (because the conductive technology is
dependent on the conductive nature of human body, it doesn’t work if the
user is wearing gloves).
2.2 Rota-Rod Features
The main features of the new model are:
▪SPEED: adjustable in the range 3-80 RPM, in steps of 1 RPM
▪MODE: constant, ramp (accelerating), reverse, multi-step ramp (NEW!)
▪ROTATION: forward, reverse and rocking
▪DRIVE: totally silent motor. Zero noise!
▪CONTROLS: 4”3 touch-screen to set and monitor the test
▪SOFTWARE: X-PAD brand new, user-friendly version, to set the experiment
and manage the results
▪DETECTION:
while maintaining the reliable
magnet-contact detection system,
the new model introduces new trip-
boxes replacing the old trip-plates.
The boxes confine the animals
when they fall off the rod and are
made of stainless steel to
guarantee sturdiness and easy
cleaning (they can be autoclaved).
Figure 2 “Trip-Box”

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47650 Instruction Manual (Rev. 2.1)
2.3 Experimental Configuration
Via the new X-PAD software, the
operator can easily organize the
experiment on her/his PC, and
upload it to the Rota-Rod via the
USB flash drive provided.
Treatments, protocols, stages,
animals, and various test features
(speed, mode, revolution, etc.) can
be quickly defined and saved for
future use.
Configurations are easily:
▪exported to Text, Excel or Pdf reports
▪saved to cloud to DropBox, OneDrive, GoogleDrive
2.4 Data Collection and Management
A basic version of the collected data can be viewed on the touch-screen; when
transferred to PC via USB drive, test results appear in full version.
The software automatically classifies the data, combining configuration settings with test
results. The user can add information, before or after the test.
Results appear in a tree-like structure, where columns can be dragged and dropped to
customize the layout.
Data are quickly:
▪exported to Text, Excel or Pdf reports
▪saved to cloud to DropBox, OneDrive, GoogleDrive
3 INSTALLATION
3.1 Unpacking & Preliminary Check
Check the contents of the shipment for completeness, packing list to hand, and visually
inspect the instrument as soon you take it out of the packaging. Use the supplied Check
List.
If the instrument is damaged, inform the carrier immediately, notifying our company. If
after having tested it, the instrument fails to meet rated performances, please contact our
after sales service, see paragraph 6.4-Customer Support.