UGO BASILE Plantar Test User manual

Instruction manual
Plantar Test
(Hargreaves method)
Revision 1.2
SKU: 37570
Pain
Inammation

SAFETY
CONSIDERATIONS
Your science, our devices
More than 35.000 citations
Although this instrument
has been designed with international
safety standards, it contains
information, cautions and warnings
which must be followed to ensure
safe operation and to retain the
instrument in safe conditions.
Service and adjustments should be
carried out by qualied personnel,
authorized by Ugo Basile organization.
Any adjustment, maintenance and
repair of the powered instrument
should be avoided as much as possible
and, when inevitable, should be carried
out by a skilled person who is aware of
the hazard involved.
Capacitors inside the instrument
may still be charged even if the
instrument has been disconnected
from its source of supply.

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MOD. 13 Rev. 1
CE CONFORMITY STATEMENT
Manufacturer UGO BASILE srl
Address Via G. di Vittorio, 2 – 21036 Gemonio, VA, ITALY
Phone n. [phone]
Fax n. [phone]
We hereby declare that
Instrument. PLANTAR TEST
Catalog number 37570
is manufactured in compliance with the following European Union Directives
and relevant harmonized standards
• 2014/35/UE relating to electrical equipment designed for use within certain voltage limits
• 2014/30/UE relating to electromagnetic compatibility
• 2011/65/UE and 2015/863/UE on the restriction of the use of certain hazardous substances in
electrical and electronic equipment
Account Manager Mauro Uboldi
Nome / Name
October 2020
Date Firma / Signature

The 37570 NEW Plantar Test
(Hargreaves’ Method) brings together tradition
and innovation.
The highly cited and world-wide recognized
thermal stimulator with automatic paw
withdrawal latency measure, has now some
key new features and benets, helping the
user to perform the experiment in a more
efcient and accurate way.
Main features:
• Automatic detection of the animal response
• Data export via USB pen-drive
• Adjustable I.R. Intensity
New features:
• Selectable automatic or manual detection
mode
• Large touch screen display for calibration,
set-up, testing and result review
• TTL input and output signals for synchroni-
sation with other devices
• Firmware update via USB key
Product features and general information

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What’s in the box Plantar Test instruction manual > Page: 5
What’s in the box
SKU: 37570
1 Control unit
1 IR emitter/detector
1 Metal base with 4 columns
1 Glass pane (to be positioned above the base)
3 Sets of animal modular enclosures (up to 12 mice or 6 rats)
1 Power cord according to your country
1 USB pen containing:
This instruction manual
The quality control and warranty certicate
OPTIONAL ITEMS ORDERING INFORMATIONS:
SKU:37300
Heat-Flux I.R. Radiometer
AVAILABLE SPARE PARTS
SKU: E-HR 002
I.R. Bulb (Halogen “Bellaphot”, Mod. 64607 OSRAM, 8V-50W)
SKU: E-BT 008
Battery type CR2032
SKU: E-FT010-1
2 X fuse T1.25A - 6X32 mm

Page: 6 < Plantar Test instruction manual Product features and general information
Product features and general information ...................................................4
What’s in the box .........................................................................5
1 General ................................................................................7
1.1 Principle of Operation ...........................................................7
2 Instrument Description ..................................................................7
2.1 Animal Welfare .................................................................8
2.2 Touch-screen command/display .................................................8
2.3 Notes on Resistive Touch-Screens ................................................9
3 Installation............................................................................10
3.1 Unpacking & preliminary check..................................................10
3.2 Notes on the Instruction Manual ................................................10
3.3 Safety Instruction .............................................................11
3.4 Assembling the instrument .....................................................11
3.5 Before Applying Power .........................................................11
3.6 Intended Use .................................................................12
3.7 Additional Safety Consideration .................................................12
3.8 Connections..................................................................12
4 Operation .............................................................................14
4.1 Main Menu ...................................................................14
4.2 Quick setup for first test .......................................................15
4.3 Set up and Utilities Icons ......................................................17
4.4 Radiometer Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
5 Bibliography...........................................................................22
6 Connections...........................................................................23
6.1 Communication port connection.................................................23
7 Maintenance...........................................................................24
7.1 Electrical .....................................................................24
7.2 Cleaning/disinfection ..........................................................24
7.3 Long Inactivity ................................................................24
7.4 Customer Support .............................................................24
8 Specication ..........................................................................25
Contents

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1 General Plantar Test instruction manual > Page: 7
1 General
The device has been designed to perform screening of analgesic drugs and rodent phenotyp-
ing via heat stimulation on the rat/mouse paw, according to the method designed by Prof K.
Hargreaves.
It basically consists of an I.R. source, whose radiant energy of adjustable intensity is focused on
the rat/mouse paw, while it is freely moving on a glass pane, over which animal enclosures are
positioned to limit its movements and eventually deliver the energy to the paws from under-
neath, when the animal is standing still.
The instrument measures the time latency of the paw withdrawal response when pain elicits it,
given the radiant heat applied to the animal’s paw.
1.1 Principle of Operation
The rat/mouse is normally placed into the animal enclosures from 15 minutes to 1-2 hours
before the test starts (habituation) and the operator waits for the end of any sign of exploration
or discomfort before starting the test itself. The amount of time varies with animal models and
species.
At this point, using the cross centred around the IR emitter, the operator targets the animal paw
and starts the stimulus by pressing the detect on the emitter or on the display.
The reaction time of the animal is scored and automatically recorded.
2 Instrument Description
The Plantar Test Unit consists of an I.R. source, whose radiant energy of adjustable
intensity is focused by a parabolic mirror on the animal paw.
The instrument components are:
The I.R. source, the sensor, the microcontroller and the electronic circuit.
On the instrument front panel are located a touch screen display and several connection ports.
On the back of the device are located the power socket, the power fuse and switch, the emitter
cable connector and the serial number label.
At the bottom of the IR emitter, is located a sticker with the factory calibration data.

Page: 8 < Plantar Test instruction manual 2 Instrument Description
2.1 Animal Welfare
In any analgesia test, great care must be taken to prevent the animal from inadvertent harm.
When an investigator initially begins using the Plantar Test unit, and when rst assessing a new
strain of animals, care must be taken to identify the appropriate amount of stimulus that will
produce the desired response and no/minimum harm.
An adjustable cut-off time (maximum stimulus time) can be set from 5” to 30” to prevent animal
injury.
2.2 Touch-screen command/display
The Plantar Test incorporates a 4.3” touch-screen display, for basic settings and monitoring, via
an intuitive Home menu.
Home menu
During the test (below gure), the touch-screen display, shows the numeric values of the set IR
intensity (in %) and the elapsed time (in seconds).
In addition, at the bottom of the Time counter, the function mode is indicated (auto or manual
detection).
Test page

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2 Instrument Description Plantar Test instruction manual > Page: 9
2.3 Notes on Resistive Touch-Screens
The Ugo Basile Plantar Test touch screen uses resistive technology and can be used with n-
gers, gloves or (recommended) a display pencil.
Resistive touch-screens (differently from commercial capacitive screens).are a better choice for
laboratory applications because of:
• High resistance to dust and water
• Better use with gloved hand or stylus

Page: 10 < Plantar Test instruction manual 3 Installation
3 Installation
3.1 Unpacking & preliminary check
Check the content of the shipment for completeness and visually inspect the instrument as
soon as you take it out of the packaging.
If the box looks damaged, inform the carrier and provide a conditional signature (not a full
signature).
Once unpacking, if the instrument is damaged, notify our company, writing an email to
[e-mail]
If after a test, the instrument fails to meet the expected behaviour and performance, please
contact our after sales service at [e-mail].
Protect the environment: Dispose of packaging properly, according to existing and applicable
waste management rules and regulation.
3.2 Notes on the Instruction Manual
This instruction manual included in the box (in the USB pen drive in PDF format) is necessary
for a correct installation and operation of the instrument.
Please save the manual, keep it ready to be consulted by the qualied personnel using the
instrument, and print it only if necessary.
Our Instruction Manuals are available as free download on our web site, www.ugobasile.com
For any additional information and/or assistance, you are welcome to contact our Service
Department, rst of all by specifying the serial number of your instrument (service@ugobasile.
com)

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3 Installation Plantar Test instruction manual > Page: 11
3.3 Safety Instruction
The following guide lines must be followed to ensure safe operation.
• DO NOT attempt to open or perform any service work before having contacted Ugo Basile
support team
• DO NOT use the device on human subjects
3.4 Assembling the instrument
Place the electronic unit on a stable and at surface and do not block the lateral ventilation
grids. To guarantee the rated performance, a free area should be left around the instrument of
at least 20 cm left, right and rear.
Then screw the 4 columns onto the metal base and position the glass frame pane onto the col-
umn themselves. At this point you are ready to assemble the modular animal enclosures (see
specic manual)
The Plantar test full system
3.5 Before Applying Power
The Power Module (see gure below ) is positioned on the left of the back panel and incorpo-
rates, from left to right, the fuse holder, the ON/OFF switch, the inlet connection of the power
cord.
Power module
The fuse compartment holds two fuses. Use (T1,25A 6X32mm) fuses for operation at both 100 or
240V, for fuse replacement, please refer to paragraph 7.1-Electrical at page 24 of this manual.
The power cord inlet ts a standard C13 cable, Cat. # E-WP008.
Make sure your power out-take is provided with a reliable ground connection.

Page: 12 < Plantar Test instruction manual 3 Installation
3.6 Intended Use
The Plantar Test is intended for investigation use on laboratory animal only.
3.7 Additional Safety Consideration
1. Use original accessories and spare parts only
2. Immediately disconnect and replace damaged main cord
3. Do not obstruct access to the power module
4. Do not obstruct the ventilation grids on booth sides of the cabinet
5. Do not operate in hazardous environment or outside prescribed environmental limitations
6. Do not spray any liquid on the connectors, display, or other parts
Ugo Basile cannot in any way and form be held responsible for damage caused to things and
people and warranty will be void, due to:
• Incorrect electrical supply
• Incorrect installation procedure
• Incorrect or improper use or, in any case, not in accordance with the purpose for which the
instrument has been designed and the warnings stated in the instruction manual supplied
with the instrument
• Replacement of original component, accessories or parts with others not approved by the
manufacturer
• Servicing carried out by unauthorized personnel
3.8 Connections
Connect the mains cord between the power socket of the Plantar Test and the power outlet with
a reliable earth connection.
Connect the emitter cable to the electronic unit and then the power cord.
At this point you can turn the Plantar test on.
Rear view Plantar Test control unit

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3 Installation Plantar Test instruction manual > Page: 13
The connection module on the front panel (Figure below) encompasses the following
connectors, from let to right:
1. Upper and lower USB ports: enable data export to a PC (via a USB pen drive), and allows
rmware upgrades
2. TTL I/O: 15pins D-SUB connector, provides TTL input and output for start/stop command
and mode settings
3. COM: Reserved for maintenance and service purpose
4. Ethernet connector: only for support reason at the factory, not to be used
Connection panel

Page: 14 < Plantar Test instruction manual 4 Operation
4 Operation
Position the Plantar Test electronic unit and base with the glass pane and the animal enclo-
sures in a quiet and at environment.
The Plantar test device
Avoid direct illumination, e.g., from a table lamp.
This will denitely upset the animals, as rodents dislike bright light, not to speak of the risk to
raise their body temperature.
Moreover, intense light may cause the sensor to sometimes deliver inaccurate results, as a light
source from above tends to mask the I.R. energy reected back by the paw.
4.1 Main Menu
Getting familiar with the Plantar Test home page menu is very easy (see gure below).
Main Menu
From the home page, press the “UB logo” button for software and memory information. Press
again the “UB logo” to roll back into main menu page.

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4 Operation Plantar Test instruction manual > Page: 15
4.2 Quick setup for rst test
Animal handling and habituation:
It is advisable to gather some experience in handling rats/mice and in developing the best
habituation protocol before the test is started, so that the pointing and the delivery of energy
to the paw will be more accurate.
You are now ready to congure and run your rst experiment. The following steps will guide you
to quickly congure the Plantar Test device and get the rst results:
Carrying out the test
Put the animal(s) in the cage(s) and wait until they are quiet, with little or no exploration, but
not sleeping.
A trial is initiated by pressing start from the emitter buttons or from the touch screen.
When the animal feels pain and withdraws its paw, the I.R. source switches off and the reaction
time counter stops. The withdrawal latency to the nearest 0.1 s is automatically detected and
stored.
A MANUAL MODE can also be used in this new Plantar Test generation for those cases where the
paw withdrawal is tricky to measure automatically. To measure latency manually, simply press
the button once to start the test and press again to stop it, and the instrument will measure the
latency time.
The start page
Initiating a test is very simple:
1. Press SETUP to adjust the IR intensity and the cut-off time
2. Press START to initiate the test through the touch screen or through one of the two buttons
located in the emitter (according to your preference)

Page: 16 < Plantar Test instruction manual 4 Operation
Looking at the results
The Plantar Test will save the IR intensity and time latency results, in addition to the descriptive
data, if they have been input in the Experiment eld (Treatment, Protocol, ID, Stage, Trial).
Result page
Experiment screen, where the descriptive data shown in the Results screen are input
Exporting the results
Once data have been saved into the Plantar Test internal memory, they can be transferred into
a USB pen drive by simply plugging it into the front USB port and pressing,
- USB STORAGE, followed by
- SAVE DATA
The export window

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4 Operation Plantar Test instruction manual > Page: 17
4.3 Set up and Utilities Icons
The “set up” icon from the home screen is intended to dene the experiment parameters, i.e.
IR intensity (level of energy delivered to the paw) and “cut off time”, ie. the time after which
the energy source will turn off even if there is no paw withdrawal (intended to avoid tissue
damage).
The set-up page
The keyboard screen to input the IR intensity in percentage values
The keyboard screen to input the cut-off value in second
The UTILITIES icon, accessible from the MAIN menu, gives access to:

Page: 18 < Plantar Test instruction manual 4 Operation
• Update
• Date-TIme
• Calibration
• Erase DB
The UPDATE menu requires to insert the USB pen drive and is necessary to updated the device
rmware. Contact the Ugo Basile Support team before updating the rmware.
The update page
The DATE-TIME menu allows to adjust the date and time of the device, as shown in the screen
shot below

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4 Operation Plantar Test instruction manual > Page: 19
The date & time page

Page: 20 < Plantar Test instruction manual 4 Operation
The CALIBRATION, menu is dedicated to the conversion of the arbitrary % units of the Plantar
Test into absolute energy values (SKU 37300).
In addition to this, when a 37300 radiometer (optional, which can be purchased from Ugo Ba-
sile) is available, the emission power of the heat source can be measured and adjusted.
Factory calibration values can be input in the menu below, by simply reading the values from
the sticker attached to the bottom of the unit.
The calibration screen
From the UTILIES screen also the full memory can be erased, by tapping onto the ERASE DB
button