UGO BASILE 57800 User manual

E C T U N I T
Cat. No. 5 7 8 0 0

UGO BASILE
BIOLOGICAL RESEARCH APPARATUS INSTRUCTION MANUAL
Via G. Borghi 43
21025 COMERIO - Varese, ITALY E C T U N I T
Cat. No. 5 7 8 0 0
Series No. Mfg. date
THIS INSTRUMENT IS WIRED FOR
115 Volts – 60 Hz
115 Volts – 50 Hz
230 Volts – 50 Hz
230 Volts – 60 Hz OPERATION
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ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL SAFETY
STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND WARNINGS
WHICH MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO RETAIN THE IN-
STRUMENT IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSONNEL,
AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UN-
DER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITA-
BLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE
HAZARD INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE IN-
STRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated March 2009
Revision 1

E C T UNIT
Cat. No. 5 7 8 0 0
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RE ES SE EA AR RC CH H A AN NI IM MA AL LS S
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Pa ar rt ti ic cu ul la ar rl ly y u us se ef fu ul l f fo or r: :- -
general screening of potentially neurotropic substances
evaluating the depressant or stimulating action of drugs on the CNS
endocrinological investigations on the relationship between the nervous system and the hypophysis.
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UGO BASILE S.R.L.
BIOLOGICAL RESEARCH
APPARATUS
CHECK-LIST
CAT. 57800 E C T UNIT
CLIENTE / CUSTOMER______________________________________________
No. Ordine / Order No. ____________________ Data / Date____/_____/______
UB code CAT.No.
 Q.ty
DESCRIPTION
 DESCRIZIONE
57800-001 1 PULSE GENERATOR GENERATORE DI IMPULSI
57800-002 1
SET OF AURICULAR ELECTRODES
SET DI ELETTRODI AURICOLARI
57800-302 1 INSTRUCTION MANUAL MANUALE DI ISTRUZIONE
E-WP 008
1 MAINS CABLE EUROPE CAVO RETE EUROPA
E-WP 008-1 U.S.A. U.S.A.
E.WP 008-2 U.K U.K
M-LM 345 57800-301 1 DUST COVER COPERTINA
E-FT 010-1 2 FUSES FOR 115V (T 1,25 A ) FUSIBILI PER 115 V (T 1,25 A)
E-FT 010-1 2 FUSES FOR 230V (T 1,25 A ) FUSIBILI PER 230 V (T 1,25 A)
E-PE 001
1 2 MM. BLACK BANANA PLUG SPINOTTO NERO 2 MM
M-LM 080-F 1 “DANGER” LABEL TARGHETTA “DANGER”
Verificare che cambio tensione e fusibili inseriti nel blocco alimentazione corrispondano ai dati di targa
Check that the voltage selector and the fuses correspond to what indicted in the rating marks
OPTIONS
57800-003 1
SET OF CORNEAL ELECTRODES
57800-320 1
SET OF 4 FELT PADS FOR 57800-003
57800-010 1
BIPOLAR INVERTER
Serial No.
57800-015 1
ECT MONITOR, including following accessories:-
Serial No.
E-WP 008 1
MAINS CABLE
E-WP 027 1
CONNECTION CABLE, 2mm PLUG
E-WP 019 1
BNC CABLE
E-WP 045 1
POWER SUPPLY
E-WP 026 1
CONNECTION CABLE, 4mm PLUG
DATE / / Serial No. IMBALLATO DA / PACKED BY
Set for
115V 50 Hz 230V 50 Hz 115V 60 Hz 230V 60 Hz
IMPORTANT/IMPORTANTE:
Check the shipment for completeness immediately after receipt: should you find any discrepancy, please fill in the following part
and transmit it to our fax No. [phone]
Al ricevimento della merce controllate che la spedizione sia completa: in caso di discrepanza, completate il formulario di seguito
riportato ed inviatelo al nostro fax No. 0332 745488
FROM: Name
Company/Institution
DATE REF.
NOTES
MOD.04 REV 0

UGO BASILE - I TALY Page i
57800 Instruction Manual (Rev. 1)
C O N T E N T S
1GENERAL.......................................................................................................................1
1.1 I
NSTRUMENT DESCRIPTION ................................................................................................1
1.1.1 P
ULSE G ENERATOR 3
1.1.2 R
ESET 3
1.1.3 S
HOCK 3
1.1.4 C
URRENT S ETTING AND “V IOLATION ” 3
1.1.5 S
HOCK P ARAMETER S WITCHES 3
1.2 E
LECTRODES ....................................................................................................................3
1.2.1 E
LECTRODE C HECKING 4
2INSTALLATION..............................................................................................................4
2.1 U
NPACKING & P RELIMINARY C HECK .................................................................................4
2.2 N
OTES ON THE I NSTRUCTION M ANUAL ..............................................................................4
2.3 B
EFORE A PPLYING P OWER ...............................................................................................4
2.3.1 M
AINS S WITCH 5
2.3.2 F
USE H OLDER 5
2.3.3 M
AINS C ORD 5
2.4 C
ONNECTIONS ..................................................................................................................5
2.5 W
ARNING .........................................................................................................................5
2.6 A
DDITIONAL S AFETY C ONSIDERATION ...............................................................................6
3OPERATION...................................................................................................................7
3.1 S
WITCHING O N .................................................................................................................7
3.2 S
ELECTING P ULSE P ARAMETERS ......................................................................................7
3.3 E
LECTRODE H ANDLING .....................................................................................................7
3.4 E
LECTRODE P OSITIONING .................................................................................................8
3.5 S
HOCK D ELIVERY .............................................................................................................8
3.5.1 S
HOCK /R ESET S EQUENCE 8
3.5.2 P
EDAL S WITCH 9
4TESTING.........................................................................................................................9
4.1 O
UTPUT M ONITORING BY CRT..........................................................................................9
4.2 O
HMETER C IRCUIT C HECK ..............................................................................................10
5CALIBRATION .............................................................................................................10
5.1 P
RELIMINARY .................................................................................................................10
6MAINTENANCE............................................................................................................11
6.1 E
LECTRICAL ...................................................................................................................11
6.2 L
ONG I NACTIVITY ............................................................................................................11
6.3 C
USTOMER S UPPORT .....................................................................................................11

UGO BASILE - I TALY Page ii
57800 Instruction Manual (Rev.1)
7ECT SPECIFICATIONS............................................................................................... 12
7.1 S
HOCK P ULSE T RAIN ..................................................................................................... 12
7.2 M
ISCELLANEOUS ........................................................................................................... 12
8ORDERING INFORMATION........................................................................................ 12
8.1 O
PTIONAL ...................................................................................................................... 13
8.2 B
IPOLAR I NVERTER 57800-010...................................................................................... 13
8.3 ECT
M ONITOR 57800-015............................................................................................. 14
8.3.1 H
OW TO U SE THE D EVICE 14
8.3.2 R
ATIO C ONVERTION 15
8.3.3 E
XAMPLES 15
8.3.4 M
ONITOR S PECS . 16
9BIBLIOGRAPHY............................................................................................................ 1
FIGURE INDEX
Figure 1 “Upper Panel”..................................................................................................... 2
Figure 2 “Power Module”.................................................................................................. 5
Figure 3 “ECT Monitor” .................................................................................................. 14
Figure 4 “ECT Monitor – Back Panel” ............................................................................ 15
Figure 5 “ECT Monitor – Front Panel”............................................................................ 15

UGO BASILE - I TALY Page 1
57800 Instruction Manual (Rev. 1)
ECTUNIT
Cat. 57800
1GENERAL
The ECT Apparatus is specially designed for neurochemical and neuropharmacological
research. It is particularly useful for:-
a) general screening of potentially neurotropic substances
b) evaluating the depressant or stimulating action of drugs on the CNS
c) endocrinological investigations on the relationship between the nervous sys-
tem and the hypophysis.
A constant current output is used which ensures reproducible results and accurate de-
termination of the EC threshold while also pinpointing any variation in the threshold
brought about by drugs having specific action on the cortex and subcortical regions.
The shock parameters have been selected after consulting the most recent literature, in
order to determinate the range of requirements likely to be needed when operating with
mice and rats.
The solid state circuit supplies from 1 to 299 rectangular pulses per second, .1 to .9 ms in
width, for duration variable from .1 to 9.9 seconds. Shock current can be accurately pre-
set on a scale of 1-99 mA.
The actual intensity of the shock depends on the current which runs through the subject’s
body. The instrument automatically adjusts the current to the value preset by the opera-
tor, irrespective of the animal’s skin resistance and skin/electrode interface conditions.
Accordingly, the intensity of the current running through each animal in any given ex-
perimental group is identical.
The standard auricular electrodes supplied allow a single operator to deliver shock to a
number of animals in a short time. The special output circuit enables any type of elec-
trode to be used, e.g., corneal or temporal electrodes, which can be provided on request.
1.1 Instrument description
The instrument consist of:-
1) a constant current Pulse Generator 57800-001, which comprises:-
a pulse train generator
a power amplifier

UGO BASILE - I TALY Page 2
57800 Instruction Manual (Rev. 1)
a pulse transformer
two power supplies
2) a set of Electrodes (auricular electrodes 57800-002 are supplied as standard)
Figure 1 “Upper Panel”

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57800 Instruction Manual (Rev. 1)
1.1.1 Pulse Generator
The Pulse Generator is lodged into a resilient cabinet of original design. Its upper panel,
see Figure 1 “Upper Panel”, features the following controls:-
1.1.2 Reset
When the apparatus is on, to deliver a shock, the circuit should first be reset by keeping
the RESET key depressed (for about 1 second) until the illuminated green legend
READY is ON.
This prevents inadvertent shock delivery due to accidentally depressing the shock key.
IF THE RESET AND THE SHOCK KEYS ARE ACCIDENTALLY DEPRESSED AT THE
SAME TIME, THERE IS NO OPERATION.
1.1.3 Shock
This red key causes the instrument to deliver the shock pulse train. Once the RESET
(green) LED is ON, the SHOCK key should be depressed throughout the duration of
the shock, which is monitored by the illuminated red legend.
The shock duration timer (monitored by the red LED) is in fact independent on the time
the key is depressed .
In other words, when releasing the shock key, you cut the power off.
This type of actuating arrangement operates as a safety device; even in the unlikely
event of an electronic breakdown, no shock can be delivered unless the output stage is
energized by the key in the depressed position.
1.1.4 Current Setting and “Violation”
The dual thumb-wheel “CURRENT” presets the shock current between 1 and 99 mA.
In the case the preset current cannot be delivered (for instance due to high animal im-
pedance or to disconnected electrodes), the VIOLATION legend and the “beeper” come
alive, see also paragraph 3.4-Electrode Positioning.
1.1.5 Shock Parameter Switches
These switches set the shock parameters, i.e., pulse width in ms, frequency in pulses per
second, shock duration in seconds and current in mA.
1.2 Electrodes
The ECT Unit is provided as standard with Auricular Electrodes 57800-002. Corneal
Electrodes 57800-003 are also available as optional, see paragraph 8-ORDERING IN-
FORMATION. Different types of electrodes are available on request.

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57800 Instruction Manual (Rev. 1)
1.2.1 Electrode Checking
The electrodes can be switch to an internal ohmmeter circuit by depressing the ECT-
LOOP CHECKING key.
The Ohmic resistance of the subject is thus displayed on the 2 ½ -digit LCD display, cali-
brated in KOhm, enabling the research worker to be sure that the electrodes are correctly
located and that contact resistance is below 20-25,000 Ohm.
The Ohmic range is 0-199 KOhm. If the load resistance is over 199 KOhm, the display
MSD (main significant digit) only (i.e., the left one) is energized and shows “I”.
2INSTALLATION
2.1 Unpacking & Preliminary Check
Check the contents of the shipment for completeness, packing list to hand, and visually
inspect the instrument as soon you take it out of the packaging. Use the Check List sup-
plied.
If the instrument is damaged or, after having tested it, fails to meet rated performances,
notify the carrier and our company immediately.
P
Pr ro ot te ec ct t t th he e e en nv vi ir ro on nm me en nt t! !
Dispose of packaging properly, according to existing and ap-
plicable waste management rules and regulations.
2.2 Notes on the Instruction Manual
The 57800 Instruction Manual included in the Electronic Unit package is necessary for
the correct installation and operation of the instrument.
We recommend keeping the manual in good conditions, ready to be consulted by the
qualified personnel who use the instrument.
Free of charge copies of the instruction manual are available upon request: please con-
tact our service department (see paragraph 6.3-Customer Support) specifying the series
number of your instrument.
2.3 Before Applying Power
Take a look at the Power Module, on the left of the Electronic Unit back-panel, which en-
compasses – from left to right - the fuse holder, the mains switch and the inlet connection
of the mains cord:

UGO BASILE - I TALY Page 5
57800 Instruction Manual (Rev. 1)
Figure 2 “Power Module”
2.3.1 Mains Switch
This two-pole toggle switch, which complies with international safety standards, provides
a visual cue, meaning:-
OFF when the “O” side is depressed
ON when the “I” side is depressed
2.3.2 Fuse Holder
The fuse holder comprises two fuses, one on the live, and the other on the neutral. For
operation at 220-230 Volts, we recommend 630 mA timed fuses (type T630). Use 800
mA fuses (type T800) for operation at 115 Volts. To replace the fuses, see paragraph
6.1-Electrical.
2.3.3 Mains Cord
It is a standard cable, Cat. # E-WP008. Make sure your power outtake is provided with a
reliable ground connection, see also 2.4 & 2.6.
2.4 Connections
Connect the mains cord to a power outtake, provided with a reliable earth connection,
protected by a differential earth-leakage switch (CGFI) that breaks at the threshold of
0.03A, within a max. acceptable delay of 1s. The maximum breaking compliance required
by the CGFI is10kA at rated current is 6A.
Connect the two electrode wires (red and black) to the corresponding socket receptacles
(following the colour scheme) on the upper panel (see Figure 1 “Upper Panel”).
2.5 Warning
The EC treatment must be carried out on an unobstructed non-conductive working sur-
face, e.g. , a wooden table, a tile covered laboratory bench, etc.
Ensure that the working surface is clean and dry. Conductive surfaces as top of metallic
desks, cabinets, etc., must be avoided.
slot indentation

UGO BASILE - I TALY Page 6
57800 Instruction Manual (Rev. 1)
The ECT output is not “floating” and consequently the current would run through the sub-
ject’s body, following “wrong” paths, in case the conductive surface happens to be
earthed. If the conductive surface is not earthed accidental contact with the operator or
other laboratory people may be really dangerous.
! WARNING !
DUE TO THE HIGH VOLTAGE INVOLVED, THE OPERATOR SHOULD
ALWAYS WEAR DRY RUBBER GLOVES WHEN HANDLING ELEC-
TRODES CONNECTED TO THE ECT UNIT IN A LIVE EXPERIMENT.
A LABEL ON THE INSTRUMENT REMINDS THAT. PLEASE DO NOT
REMOVE THIS PLATE
2.6 Additional Safety Consideration
UGO BASILE DOES NOT ACCEPT ANY RESPONSIBILITY FOR PROB-
LEMS OR HARM CAUSED TO THINGS OR PERSONS ARISING FROM:

INCORRECT ELECTRICAL SUPPLY;

INCORRECT INSTALLATION PROCEDURE;

INCORRECT OR IMPROPER USE OR, IN ANY CASE, NOT IN AC-
CORDANCE WITH THE PURPOSE FOR WHICH THE INSTRUMENT
HAS BEEN DESIGNED AND THE WARNINGS STATED IN THE IN-
STRUCTION MANUAL SUPPLIED WITH THE INSTRUMENT;

REPLACEMENT OF ORIGINAL COMPONENTS, ACCESSORIES OR
PARTS WITH OTHERS NOT APPROVED BY THE MANUFAC-
TURER;

SERVICING CARRIED OUT BY UNAUTHORIZED PERSONNEL.
a. Place your ECT Unit 57800 on a steady flat surface (e.g. your experimentation
table).
b. Do not obstruct free and comfortable access to the power module.
c. Use original accessories and spare parts only, see also paragraph 8-
ORDERING INFORMATION.
d. Immediately disconnect and replace a damaged mains cable.
e. Do not operate in hazardous environments or outside prescribed environ-
mental limitations (i.e. +10C° / +40C°, 95% relative humidity, non-condensing),
see also paragraph 12-ECT SPECIFICATIONS.
f. Do not spray any liquid on the connectors; see also paragraph 6-
MAINTENANCE.

UGO BASILE - I TALY Page 7
57800 Instruction Manual (Rev. 1)
3OPERATION
3.1 Switching On
Switch on the 57800 by acting on the Mains Switch placed on the back side of the in-
strument; see paragraph 5-Mains Switch and in particular Figure 2 “Power Module”.
3.2 Selecting Pulse Parameters
An ample selection is provided (see 7) to enable the research worker to reproduce most
experimental schedules quoted in the literature.
As a suggestion, to get familiar with the instrument, the following parameters could be se-
lected for a threshold EC seizures in a 25-30 g mouse:-
Current : 15-18 mA
Shock Duration : 1 s
Frequency : 100 pulses per second
Pulse Width : 0.5 ms
3.3 Electrode Handling
! WARNING !
DUE TO THE HIGH VOLTAGE INVOLVED, THE OPERATOR
SHOULD ALWAYS WEAR DRY RUBBER GLOVES WHEN HAN-
DLING ELECTRODES CONNECTED TO THE ECT UNIT IN A LIVE
EXPERIMENT.
A PLASTIC PLATE ATTACHED TO ONE INSTRUMENT SIDE RE-
MINDS THAT. PLEASE DO NOT REMOVE THIS PLATE
The ear electrodes should be dipped in Ringer or similar salty solution to soak the felt
pads before clipping them to the animal’s ears, to decrease contact resistance, which will
further drop if grease and dirt are removed from the ears with cotton wool and ether be-
fore starting an experiment. In alternative, smear some conductive gel (e.g. the one
commonly used for ECG practice) to the felt-pads on the ear electrodes.
The latter provision is not indispensable, since the subject’s resistance is never a major
problem: the main feature of the apparatus, the constant current output, ensures repro-
ducible results irrespective of the position of the electrodes and skin/electrode interface
conditions.
However, by providing the least possible load resistance, the output voltage for a given
current set will also be the minimum possible, because of the Ohm’s law.
This makes the whole experimenting safer and less demanding for the ECT Unit high
voltage components.

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57800 Instruction Manual (Rev. 1)
3.4 Electrode Positioning
It is convenient to restrain the animal by holding its neck skin between forefinger and
thumb and pressing it gently against the table, while applying the electrodes with the
other hand.
We suggest to release the animal before administering the shock, as it is safer the
operator hand to be far from the electrodes: despite the gloves, extra caution helps when
handling high voltages.
Moreover, when convulsions take place, experience has shown that the animal may get
hurt when restrained even by the hand of the operator, not to speak of any other holder,
cubicle, tight harness, etc., which are definitely unsuitable.
When operating with mice, because of their small size and extreme motility, as soon the
operator releases the animal, it may attempt to turn or jump or both in the legitimate effort
to get rid of the electrodes. In this way the electrodes may come in contact over its head.
The instrument is protected against short circuits, so no damage will arise, but – of
course – in case of a “short”, there will be no ECT!
Experience will teach how to avoid shorts, how to prevent the wires to get entangled, etc.
Some “dry runs” may also help to practice the technique of holding the animal, placing
the electrodes, etc.
In the early stage of any experimental schedule, it is advisable to check the subject’s re-
sistance (see also 1.2.1 & 3.3). Do not get discouraged if you read 30-50,000 Ohm which
may lead you to think, after a check on your pocket calculator, that a current of, say, 90
mA is unattainable.
The Ohmic resistance of the subject decreases in fact by a factor of 3-4 after the first
shock pulse.
In any case, during the shock phase, if the preset current and the load are incompatible
(see also 1.1.4), the yellow VIOLATION legend and the beeper come alive.
3.5 Shock Delivery
3.5.1 Shock/Reset Sequence
To deliver the shock, in case the green legend is OFF (see also paragraph 1.1.2-Reset),
the circuit should be reset by holding the RESET key depressed for about one second.
The green legend lights, indicating that the apparatus is READY. Once the reset is done,
depress the SHOCK key and hold it thoroughly depressed until the red legend SHOCK
goes out (see also paragraph 1.2).

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57800 Instruction Manual (Rev. 1)
3.5.2 Pedal Switch
The shock can be delivered by an optional pedal switch, Cat. 57800-005, branched via
the connector placed on the instrument back. The pedal switch which performs, from the
circuit angle, the same function of the SHOCK key. See 3.5.1.
The pedal switch leaves both hands of the operator free, which may be
helpful when using corneal electrodes, but involves extra risk.
EXERT EXTRA CAUTION!
4TESTING
4.1 Output Monitoring by CRT
Being the ECT output a pulse signal, it is not possible to check its correct operation with
a conventional volt/ammeter: the use of a CRT is indispensable to monitor shape, width
and intensity of the pulses; moreover it is necessary to rely on a voltage divider, to han-
dle a voltage which is not compatible with conventional oscilloscopes.
To monitor the output signal, connect first a non-inductive resistor, 10,000 Ohm, 10W,
across the shock socket receptacles (red & black), to provide a convenient load. To test
at max. power parameters, i.e., max. duration, max. current, etc., the load resistor power
should go up to 30W. Three pieces 33 KOhm, 11 W, easily available on the market, con-
nected in parallel, will do the job.
Then pick up the voltage to be monitored by CRT from the black socket receptacle on the
front panel and the small black jack nearby (GND). Adjust the oscilloscope scale on
0.5V/division, .1 ms time base.
Operate the ECT Unit, setting shock duration to a somewhat large value (6 s) to provide
a sufficient monitoring time. 0.2 Volt displayed on the CRT corresponds to a current
through the load of 10 mA. Maximum displayed voltage is 1.98 Volts, corresponding to 99
mA.
Pulse parameters as set by thumbwheel switches (see paragraph 1.1.5-Shock Parameter
Switches) can be nicely visualized and measured; current adjusting is possible by acting
on the appropriate trimmer pot., located on the main circuit board..
A simpler and safer way to monitor the ECT output is combining the ECT
with our ECT Monitor Cat. No. 57800-015, see paragraph 8.3-ECT Monitor
57800-015.
This useful accessory enables the researcher to connect the ECT Unit to an
oscilloscope or a data acquisition system, without risking any damage due
to accidental wrong connections.

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57800 Instruction Manual (Rev. 1)
4.2 Ohmeter Circuit Check
The ECT circuit check can be carried out after a five minutes warm-up time.
Depress the ECT-loop checking key (no matter whether the instrument is on READY or
not).
W A R N I N G : DO NOT PERFORM ECT-LOOP CHECKING DURING
SHOCK DELIVERY!
The diplay will indicate one-blank-blank (see also paragraph 1.2.1-Electrode Checking)
on the KOhm scale, which means over 200 KOhm.
In fact, there is no “load” across the shock socket receptacles. Connect the electrodes to
the socket receptacles by the appropriate fork terminals and short them, for instance by
holding them together by 3-4 turns of small gauge copper wire wrapped around.
Depress the key again: the pointer will mark zero-zero KOhm.
For a possible zero adjusting, in case of deviation (e.g., the display shows zero-one), act
on the S.Z. (set zero) trimmer pot. located aside the display.
As a further check, connect a 10,000 Ohm resistor across the socket receptacles. The
display, when depressing the key, will indicate one-zero.
The purpose of this Ohmmeter is in fact to ascertain the continuity of the shock circuit
and to give an order of magnitude of its resistance.
A mispositioned electrode, broken electrode wire, loose fork connection, dry felt pad/s,
short circuit, etc., would otherwise be difficult to spot in the course of an experiment (see
also paragraphs 1.2.1, 3.3 & 3.4.
5CALIBRATION
5.1 Preliminary
Both frequency and duration of pulses are synchronized with the mains frequency, there-
fore they do not need any adjustment.
At 1-2 year intervals, it is convenient to check the current scale exactitude.
In case a calibration is necessary, it must be carried out at our laboratory only. Cali-
bration involves working inside the unit where AC power is available at several points
when the upper panel is removed; personal injury or death may result from contact with
high voltage circuits.
Please get in touch with our service department, see 6.3-Customer Support.

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57800 Instruction Manual (Rev. 1)
6MAINTENANCE
While any service of the instrument is to be carried out by Ugo Basile personnel or by
qualified personnel, authorized by UGO BASILE organization, this section of the instruc-
tion manuals describes normal maintenance procedures which can be carried out at the
customer’s facilities.
UNPLUG THE MAINS CORD BEFORE CARRYING OUT ANY MAINTE-
NANCE JOB!
6.1 Electrical
To inspect and/or replace the fuses, disconnect the mains cable first! Insert a minia-
ture screwdriver in the slot indentation, see paragraph 2.3, and snap out the slide which
houses the fuses.
For operation at 220-230 Volts, we recommend 630 mA timed fuses (type T630). Use
800 mA fuses (type T800) for operation at 115 Volts.
Snap in the fuse slide: the mechanical “click” ensures that it is locked. Check the voltage
flag before applying electrical power.
6.2 Long Inactivity
The instrument does not require any particular maintenance after long inactivity, except
cleaning.
6.3 Customer Support
For any further information you may desire concerning the use and/or maintenance of the
Dynamic Plantar Aesthesiometer, please do not hesitate to get in touch with our local dis-
tributor or with our service department at:-
UGO BASILE
Viale G. Borghi 43
21025 COMERIO – Varese, ITALY
Phone : [phone]
Fax : [phone]
@ e-mail : [email protected]
We recommend you to get in touch with our service department before sending any in-
strument to our factory for repair.

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57800 Instruction Manual (Rev. 1)
7ECT SPECIFICATIONS
7.1 Shock Pulse Train
Rectangular Positive Pulses : by H.V. Transformer
Constant Current : by feedback network
Pulse Rise & Fall Time : 20 µs
Pulse Width (ms) : 0.1 to 0.9 in 0.1 ms steps ± 1%
Frequency (pulses/s) : 1 to 299 in 1 pulse/s steps ± 1%
Shock Duration : 0.1 to 9.9 in 0.1 s steps ± 1%
Pulse Voltage : max. 2.5 KV
Current Range : 1 to 99 mA in 1 mA steps ± 2%
Output Load (at max current) : min. 0 Ohm – max. 25 KOhm
KOhm Display : 0 to 199 KOhm, 1 KOhm resolution
7.2 Miscellaneous
Commands : via thumb-wheels
Read-out : liquid-crystal display
Starting : via keys on the electronic unit panel
Power Requirement: 115 or 230 V, 50/60 Hz, 75 W max.
Operating Temperature : 15° to 30° C
Sound Level : < 70 dB (A)
Dimensions : cm 26 (w) x 13 (d) x 12 (h)
Total Weight : 10.20 Kg
Shipping Weight : 20.00 Kg approx.
Packing Dimensions : 80 x 60 x 44 cm
8ORDERING INFORMATION
57800 ECT Unit, new model, standard package including
57800-001 Pulse Generator
57800-002 Set of Auricular Electrodes
57800-301 Dust Cover
57800-302 Instruction Manual
57800-320 Set of Felt Pads for Auricular Electrodes

UGO BASILE - I TALY Page 13
57800 Instruction Manual (Rev. 1)
E-WP008 Mains Cable
Set of 2 fuses for either 115 VAC or 230 VAC mains
8.1 Optional
57800-003 Set of Corneal Electrodes
Different types of electrodes are available on request.
57800-010 Bipolar Inverter
57800-015 ECT Monitor, complete with connection cables
8.2 Bipolar Inverter 57800-010
The purpose of the optional Inverter 57800-010 is to provide biphasic output, i.e., to al-
ternate the polarity of the pulses delivered by ECT Unit 57800.
Because of the upper frequency operational limit of the reed-delays, the combination
ECT-INVERTER can operate up to 100 Hz maximum.
The Inverter has its own power supply and relative mains connection. Its mains switch
and fuses (T250mA) are located on the back panel.
A small-gauge cable comes out from the back panel, provided with a LEMO 4-pin con-
nector at its free end. This connector should be plugged to the ECT Unit’s matching con-
nector located on the back panel.
This cable brings the synchronizing signal to the Inverter, to operate its reed-relay
switches.
The ECT output (a bipolar cable is provided) should be connected to the Inverter socket
receptacles marked FROM ECT 57800).
The inverter socket receptacles marked OUTPUT will be connected to the electrodes
choosen by the experimenter, either auricular or corneal.
Both ECT Unit and Inverter should obviously be ON for the biphasic operation. If the In-
verter is accidentally OFF, there is no output. Switching on both instruments does not in-
volve any priority problem, so to speak. Either one can be switched on fist.
The VIOLATION and ECT LOOP checking functions are operative even when the output
passes through the Inverter.
During the shock phase, the Inverter buzzes at moderate but audible acoustic level. It is-
the reed-relay operation which involves the vibration of metal reeds, inside glass am-
poules. This slight noise doesn’t mean “something is wrong”: it is physiological.

UGO BASILE - I TALY Page 14
57800 Instruction Manual (Rev. 1)
8.3 ECT Monitor 57800-015
The Ugo Basile ECT Monitor has been designed to check (via an Oscilloscope) and/or
to acquire (via a Data Acquisition System) the output signal of an UGO BASILE ECT
Unit 57800.
The 57800-015 is fully iso-
lated from the ECT Unit, in
order to avoid ground loop
and electrical shock.
With the multi-position se-
lector, it is possible to
change the gain ratio be-
tween the ECT current
signal, and the voltage
output signal of the
57800-015.
Gain up to x50 allows one
to measure even small
current across the elec-
trodes.
Figure 3 “ECT Monitor”
8.3.1 How to Use the Device
Before operating the 57800-015, a simple setup procedure is necessary.
Connect the ECT unit to the Monitor 57800-015 using the cables provided: the larger ca-
ble connects to the “black socket receptacle”, the smaller one to the “ECT ground”
socket, see Figure 4 “ECT Monitor – Back Panel”.
POWER
SU
PPLY ECT
G
R
OU
ND ECT BLACK
SOC
KET