UGO BASILE 37370 User manual

UGO BASILE S.R.L.
[email protected] / [e-mail] www. ugobasile .com
instruction manual
Plantar Test ( Hargreaves Apparatus )
Cat. No. 37370

UGO BASILE S.R.L.
Via Di Vittorio, 2
21036 GEMONIO, VA, ITALY
Phone: [phone]
instruction manual
Plantar Test
Cat. No. 37370
[email protected] / [email protected]
www. ugobasile .com
Serial No.
SAFETY CONSIDERATIONS
ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL SAFE-
TY STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND WARN-
INGS WHICH MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO RETAIN
THE INSTRUMENT IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSON-
NEL, AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UN-
DER VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITA-
BLE, SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE
HAZARD INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE IN-
STRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated October 2013
Revision 0

www.ugobasile.com
Plantar Test
(Hargreaves Apparatus)
General
Determination of acute nociceptive thermal threshold in laboratory animals has primarily relied upon the tail ick and
hot plate methods.
Although both methods are used frequently in pharmacological studies, they are not without limitation. In addition,
neither method has been extended to investigating behavioural responses to hyperalgesia.
The Plantar Test represents a remarkable advance in methodology, as it combines the best features of all other methods
of measuring pain sensitivity. Unique to the Plantar Test, the animal is unrestrained and unhandled during experi-
ments.
PAIN and INFLAMMATION
For Rats
Cat. No. 37370
AUTOMATIC
MEASUREMENT OF THE
ANIMAL RESPONSE
Main Features
l Automatic detection of paw with-
drawal (no visual score needed!)
l I.R. intensity adjustable in the
interval 01-99 (in one digit steps)
l Software included
l Modular animal enclosure, from 3 to 12 spaces,
conveniently designed to restrain mice or rats
l Optional 37300 Radiometer for calibration
l Data portability via the included memory key
l NEW: orofacial stimulation by optional holders
For Mice
Ugo Basile: more than 10,000 citations

CHECK-LIST
Cat. No. 37370 Plantar Test
(Hargreaves Apparatus)
CLIENTE / CUSTOMER______________________________________________
Ordine No. / Order No. ____________________ Data / Date____/_____/______
UB code CAT.No.
 Q.ty DESCRIPTION DESCRIZIONE
37370-001 1 CONTROL UNIT UNITÀ DI CONTROLLO
37370-002 1 MOVABLE I.R. SOURCE UNITÀ DI STIMOLAZIONE I.R.
37000-003 1 COMPLETE BASE BASE COMPLETA
37370-327 4 SUPPORTING COLUMNS COLONNE DI SUPPORTO
37370-005 1 FRAMED GLASS PANE PANNELLO IN VETRO CON CORNICE
3 x M-S 085 37000-006 1 MODULAR ANIMAL ENCLOSURE
(3 MODULES) GABBIA MODULARE (3 MODULI)
E-WP 008 1 MAINS CABLE EUROPE CAVO RETE EUROPA
E-WP 008-1 USA USA
E-FF 002 2 FUSES (2AF) FUSIBILI (2AF)
E-AU 041 USB
pen-drive 37370-302 1 INSTRUCTION MANUAL MANUALE DI ISTRUZIONE
52050-10 CUB SOFTWARE SOFTWARE CUB
52010-323 1 USB CABLE CAVO USB
OPTIONAL
37000-145 1 PANEL MOUNT PRINTER STAMPANTE A PANNELLO
37400-305 1 PACKAGE OF 10 PAPER ROLLS PACCO DA 10 ROTOLI CARTA
37370-278 1 ADDITIONAL STIMULATION BASE BASE STIMOLAZIONE AGGIUNTIVA
DATE / / Serial No. IMBALLATO DA / PACKED BY
Universal Input 85-264 VAC, 50-60Hz
IMPORTANT/IMPORTANTE:
Check the shipment for completeness immediately after receipt: should you find any discrepancy, please fill in the following part and transmit it to
our fax no. [phone]
Al ricevimento della merce controllate che la spedizione sia completa: in caso di discrepanza, completate il formulario di seguito riportato ed in-
viatelo al nostro fax no. 0332 745488
FROM: Name Company/Institution
DATE REF.
NOTE
MOD.04 REV 0

Page i
37370 Instruction Manual (Rev. 0)
C O N T E N T S
1GENERAL ..........................................................................................................1
1.1PRINCIPLE OF OPERATION .....................................................................................1
2INSTRUMENT DESCRIPTION...........................................................................2
2.1THE MOVABLE I.R. SOURCE (HEAT STIMULATOR)...................................................2
2.2THE PLATFORM/GLASS-PANE/ENCLOSURE ASSEMBLY ...........................................2
2.2.1ANIMAL ENCLOSURE 3
2.3CONTROLLER .......................................................................................................3
2.3.1COMMAND DISPLAY MODULE 3
2.3.2GRAPHIC DISPLAY 4
2.3.3KEYBOARD 4
2.3.4FUNCTION KEY ABBREVIATIONS 4
3DATA ACQUISITION & DATA PRINT-OUT ......................................................5
3.1DATA PORTABILITY ...............................................................................................5
3.2PANEL-MOUNT PRINTER 37000-145......................................................................6
3.357145 MINIPRINTER .............................................................................................6
4INSTALLATION .................................................................................................6
4.1UNPACKING &PRELIMINARY CHECK ......................................................................6
4.2NOTES ON THE INSTRUCTION MANUAL ....................................................................7
4.3ASSEMBLING THE EQUIPMENT................................................................................7
4.3.1ASSEMBLING &POSITIONING THE ENCLOSURE 7
4.3.2HOW TO HANDLE THE PLANTAR TEST 7
4.4ENVIRONMENT ......................................................................................................8
4.5BEFORE APPLYING POWER....................................................................................8
4.5.1FUSE HOLDER 8
4.5.2MAIN SWITCH 8
4.5.3MAINS CORD 9
4.6CONNECTIONS ......................................................................................................9
4.6.1BACK PANEL 9
4.7INSTALLATION OF CUB SOFTWARE ......................................................................10
4.8INTENDED USE ....................................................................................................10
4.9GENERAL SAFETY INSTRUCTIONS.........................................................................10
4.10ADDITIONAL SAFETY CONSIDERATION ..................................................................10
5OPERATION ....................................................................................................11
5.1SWITCHING ON...................................................................................................11
5.2DISPLAY CONTRAST ............................................................................................11
5.3SETTING I.R. INTENSITY.......................................................................................11
5.4CARRYING OUT THE TEST ON THE ANIMAL.............................................................12
5.4.1NOTE ON WET SURFACES 12
5.5DURHAM HOLDERS FOR OROFACIAL STIMULATION ................................................12

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37370 Instruction Manual (Rev. 0)
5.6HOW TO SET A CUSTOMIZED EXPERIMENTAL CONFIGURATION............................... 12
5.6.1SEQUENCE NUMBER 13
5.6.2EXPERIMENT NUMBER 14
5.6.3ANIMAL GENDER 14
5.6.4CUT-OFF TIME 14
5.7CONFIGURATION FILE ......................................................................................... 14
5.7.1FROM THE MEMORY KEY TO THE INSTRUMENT 15
5.7.2UPLOADING 15
5.7.3DOWNLOADING 16
5.7.4FROM MEMORY KEY TO PC 16
5.7.5FROM PC TO INSTRUMENT 16
6OPERATING WITH MINI PRINTER AND PC.................................................. 16
6.1HOW TO SET THE DATA OUTPUT TO PC ................................................................ 16
6.2HOW TO SET THE DATA OUTPUT TO MINIPRINTER.................................................. 16
6.3DATUM FORMAT ................................................................................................. 17
6.4QUICK SETTING OF THE 37370 PLANTAR TEST ..................................................... 18
7MEMORY MANAGEMENT .............................................................................. 19
7.1MEMORY RESET ................................................................................................. 20
7.1.1“MEMORY FULL”MESSAGE 20
7.2SETTING TIME &DATE ........................................................................................ 21
8REPRODUCIBILITY OF THE TEST ................................................................ 21
9CALIBRATION ................................................................................................ 21
9.1RADIOMETER...................................................................................................... 22
9.2CALIBRATION VIA THE 37300 I.R. RADIOMETER.................................................... 22
9.3CALIBRATION WITHOUT I.R. RADIOMETER ............................................................ 23
10MAINTENANCE............................................................................................... 23
10.1ELECTRICAL....................................................................................................... 24
10.1.1BATTERY REPLACEMENT 24
10.2REPLACING THE BULB ........................................................................................ 25
10.3CLEANING THE OPTICS THOROUGHLY .................................................................. 25
10.4CLEANING THE GLASS PANE............................................................................... 26
10.5REPLACING THE GLASS PANE.............................................................................. 26
10.6LONG INACTIVITY................................................................................................ 26
10.7CUSTOMER SUPPORT ......................................................................................... 26
11INSTRUMENT SPECIFICATIONS................................................................... 27
12ORDERING INFORMATION............................................................................ 28

12.1SPARE PARTS .....................................................................................................28
12.2OPTIONAL ..........................................................................................................28
12.3THERMAL MINIPRINTER .......................................................................................29
13BIBLIOGRAPHY ..............................................................................................29
13.1METHOD PAPER..................................................................................................29
13.2PAPERS MENTIONING UGO BASILE PLANTAR TEST ...............................................29
13.3DURHAM HOLDERS FOR OROFACIAL STIMULATION ................................................30
14APPENDIX .........................................................................................................1
FIGURE INDEX
Figure 1 “I.R. Source”......................................................................................................................2
Figure 2 “Panel-Mount Printer and USB Pen-Drive” .......................................................................6
Figure 3 “Power Module”.................................................................................................................8
Figure 4 “Back Panel”......................................................................................................................9
Figure 5 “Acquired Data Window”.................................................................................................17

Page 1
37370 Instruction Manual (Rev. 0)
Plantar Test (Hargreaves Apparatus)
Cat. 3 7 3 7 0
1 GENERAL
The Hargreaves’ Method (see paragraph 12-BIBLIOGRAPHY) enables the researcher
to discern a peripherally mediated response to thermal stimulation caused by drugs in the
unrestrained rat.
The Basile Plantar Test has been designed to perform this high sensitivity in-vivo test on
the experimental animal under optimum conditions.
The Plantar Test represents a remarkable advance in methodology, as it combines the
best features of all other methods of measuring pain sensitivity:
it allows bilateral testing, like the Randall-Selitto (paw pressure) method.
the behavioral end point is detected automatically, as with the Tail Flick method.
it stimulates the glabrous normal skin of the foot, as opposed to the unique tissue
of the tail, as does the Hot Plate method.
repeated testing does not result in sensitization, an advantage shared only with
the Tail Flick method.
orofacial stimulation, by optional holders, see paragraph 5.5.
Unique to the Plantar Test, the animal is unrestrained and unhandled during experi-
ments, which enables other more subjective measures of behaviour to be recorded.
These measures, unavailable with any other method of analgesia testing, provide addi-
tional information about the effect of the drug and the state of the animal.
1.1 Principle of Operation
The Plantar Test basically consists of a movable I.R. (infrared) generator placed below a
glass pane upon which the operator deposits the rat, see picture. A Perspex enclosure
defines the space within which the animal is unrestrained. It is divided in three compart-
ments, which help the operator to carry out a rapid “screening” work: up to 12 animals
can be tested with no appreciable delay in between.
After the acclimation period, the I.R. source placed under the glass floor (see the picture)
is positioned by the operator directly beneath the hind paw. A trial is commenced by de-
pressing a key, positioned on the stimulating unit. An additional start key is located on the
front panel of the Controller.

Page 2
37370 Instruction Manual (Rev. 0)
When the rat feels pain and withdraws its paw, the I.R. source switches off and the reac-
tion time counter stops. The withdrawal latency to the nearest 0.1s is automatically de-
termined.
2 INSTRUMENT DESCRIPTION
The Instrument consists of:
1) a movable I.R. Source, see paragraph 2.1
2) a Platform/Glass-Pane/Animal-Enclosure Assembly, see paragraph 2.2
3) a Controller lodged in a cabinet of original design, incorporating a Co-
mand/Display Module, see paragraph 2.3.
2.1 The Movable I.R. Source (Heat Stimulator)
The I.R. Source 37370-002 is lodged into a cy-
lindrical vessel of aluminum, fitted with a side
handle, which the operator glides over the sur-
face of the base platform (see figure 1).
At both sides of the handle (1), a START key is
provided, to help both left (2) and right (3)
handed operators.
The cylinder contains a halogen bulb coupled to
an I.R. filter, which cuts off the visible part of the
spectrum. The bulb is cooled by a quietly oper-
ating fan.
When the rat feels pain and withdraws its paw,
a suitable electronic circuitry switches off the
bulb and to stop the second counter, thus de-
termining the withdrawal latency to the nearest
0.1 second.
Figure 1 “I.R. Source”
2.2 The Platform/Glass-Pane/Enclosure Assembly
The I.R. Vessel glides on the large base platform 37000-003. The Glass Pane 37370-
005 is inserted into a consistent frame, whose external dimensions are 86(w)x35(d)cm,
held in position by its own weight on the top of the four supporting columns 37370-327,
fitted to the platform.
3 2
1

Page 3
37370 Instruction Manual (Rev. 0)
2.2.1 Animal Enclosure
A modular enclosure 37000-006 of
original design, combines 3 animal
cages.
Removable partitions enable the us-
er to divide each of the 3 cages into
2 or 4 spaces, thus obtaining up to
12 spaces.
The enclosure is duly held in position
on the framed glass pane, by the
spacers provided.
Six separate lids make it easier to de-
posit/remove the rats individually.
The cage dimensions are approx.
20(d)x62(w)x14(h)cm.
Each of the 3 compartment is hence
dimensioned 20x20x14(h)cm.
2.3 Controller
The controller front panel encompasses the COMMAND-DISPLAY MODULE, see para-
graph 2.3.1 and the slot for the memory key, see paragraph 3.1-Data Portability.
The back panel, see paragraph 4.6.1, embodies the power module and the connection
module, see also paragraph 4.6.
2.3.1 Command Display Module
This module of original design is located on the instrument front panel, and comprises a
graphic display and a command keyboard.
The multifunction liquid-crystal graphic-display monitors the withdrawal latency to the
nearest 0.1 second; latency time is the time elapsing from the moment the START key is
activated to the moment the animal withdraws its paw.
Removable
partitions
Separate
Lids